Perbedaan Efektivitas Minyak Atsiri Buah Zaitun (Olea europaea L.) Dan Minyak Atsiri Cengkeh (Syzygium aromaticum L.) Terhadap Pertumbuhan Bakteri Aggregatibacter actinomycetemcomitans

Latar Belakang: Periodontitis adalah penyakit yang mempengaruhi struktur gigi pada jutaan orang di dunia dan merupakan penyakit multifaktorial yang disebabkan oleh bakteri yang tumbuh padabiofilm di margin gingiva. Aggregatibacter actinomycetecomitans adalah salah satu bakteri yang paling sering ditemukan dalam penyakit periodontitis. Pengobatan yang paling efektif adalah dengan menggunakan bahan antibakteri. Diantara bahan antibakteri alami adalah minyak cengkeh (Syzygium aromaticum L.)dan minyak zaitun (Olea europaea L.) . Tujuan: Untuk mengetahui perbedaan efektivitas minyak atsiri buah zaitun (Olea europaea L.) dan minyak atsiri cengkeh (Syzygium aromaticum L.) terhadap pertumbuhan Aggregatibacter actinomycetemcomitans. Metode: Penelitian ini merupakan penelitian eksperimental murni laboratoris dengan metode post-test control group design. Subyek penelitian ini adalah minyak atsiri buah zaitun (Olea europaea L.) dan minyak atsiri cengkeh (Syzygium aromaticum L.) yang diperoleh dari isolat murni Laboratorium Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gajah Mada. Daya antibakteri minyak atsiri buah zaitun (Olea europaea L.) konsentrasi 0%, 50% dan 100% sedangkan minyak atsiri cengkeh (Syzygium aromaticum L.) konsentrasi 0%, 50% dan 100%. Diuji dengan menggunakan metode difusi sumuran. Media yang digunakan adalah Mueller Hinton Agar yang kemudian dibuat sumuran diameter 6mm kemudian ditetesi minyak atsiri buah zaitun (Olea europaea L.) dan minyak atsiri cengkeh (Syzygium aromaticum L.) masing-masingdengankonsentrasi 0%, 50% dan 100%. Kemudiandiinkubasi selama 24 jam dengan suhu 37oC. Zona radikal yang terbentuk dihitung menggunakan vernier calipers. Hasil: Minyak atsiri cengkeh (Syzygium aromaticum L.) memiliki daya antibakteri yang lebih efektif daripada minyak atsiri buah Zaitun (Olea europaea L.) dengan rata-rata diameter zona radikal terbesar yang terbentuk 19,98 mm (minyak atsiri cengkeh) dan 15,54 (minyak atsiri buah zaitun). Konsentrasi optimum yang dapat menghambat pertumbuhan bakteri Aggregatibacter actinomycetemcomitans adalah konsentrasi 100% dengan kemampuan sebesar 80.02% untuk minyak atsiri buah zaitun (Olea europaea L.) dan sebesar 84.46% untuk minyak atsiri cengkeh (Syzygium aromaticum L.)

Incidence of dairy wastewater on morphological and physiological comportment of Chemlali and Chetoui olive

In the present study the analysis of the physico-chemical parameters of the collected TWW shows the BOD, COD, COT, Cl-, NO3, NO2, suspended matter, organic matter, turbidity and conductivity were in accordance with the required Tunisian legislations. In the same occurrence, the ICP-MS and the UPLC-MS/MS analysis show that TWW were devoid of different toxic metals and antibiotics, respectively. We opted to reuse of the TWW in the irrigation of young olive trees of two varieties: Olea europaea L. cv. Chetoui and Olea europaea L. cv. Chemlali which receiving 1 L/week of TWW during five months. Results show that dry roots weight and the content of chlorophyll a in 'Chetoui' variety increased significantly (p < 0.05) when compared to the control group. On the same way, significantly increase of leaf area, dry roots and leaves weights was observed in 'Chemlali' variety treated with TWW. Keywords: Dairy wastewater, Reuse wastewater, Olea europaea L., ICP-MS, UPLC-MS/M

The role of Olea Europaea L. Fruit on A2780, A172 and HFFF2 proliferation

Olea europaea L. commonly known as olive has been traditionally used for the prevention and treatment of many diseases since ancient times. Olive has been reported to possess a broad spectrum of pharmacological properties. In the present study, we investigated the activity of aqueous extract of Olea europaea L. fruit at various concentrations on A2780, A172, and HFFF2 cell lines proliferation by MTT assay. Aqueous extract of olive significantly increased cell proliferation in a dose dependent manner in the cell lines. It has been previously reported that olive has chemoproventive and anti-tumor effects. These disagreements can be explained by differences in cell line properties, type of olive and different solvents in the extracts. However, further investigation is needed to clarify the exact role of olive in cell proliferation and cancer. In this study fruit extract of Olea europaea L. showed more activatory effects on A2780 cell line in comparison with A172 and HFFF2. These differences in the activatory effects may be related to the activation of different signaling pathways in different cell lines. © 2016, Iranian Association of Pharmaceutical Scientists. All rights reserved

Nutritive Value of Three-Stage Olive Cake (Olea europaea L.) for Growing Rabbit

Nutritive Value of Three-Stage Olive Cake (Olea europaea L.) for Growing Rabbit. 4. International Conference on Animal nutrition and disease

A Review Article: Potential Anti-cholesterol of Extract Olive Oil (Olea europaea L.)

Dyslipidemia is a risk factor that triggers the emergence of cardiovascular disease, resulting in many deaths worldwide. This review article aims to examine the anti-cholesterol potential of olive oil extract (Olea europaea L.). This study is a literature review to explore the anti-cholesterol potential of olive oil extract (Olea europaea L.). The results of this literature review show several papers that discuss the pattern of fat consumption much higher than others where subjects experience an increase in HDL levels caused by olive oil extract. In addition, increasing HDL cholesterol levels can reduce LDL and total cholesterol levels in the blood. Therefore, using Olive oil can be recommended as an ingredient to reduce the problem of dyslipidemia

Heavy metal bioaccumulation by the important food plant, olea europaea L., in an ancient metalliferous polluted area of Cyprus

Aspects of the bioaccumulation of heavy metals are reviewed and possible evidence of homeostasis is highlighted. Examination and analysis of olive (Olea europaea L.) trees growing in close proximity to a copper dominated spoil tip dating from at least 2000 years BP, on the island of Cyprus, revealed both bioaccumulation and partitioning of copper, lead and zinc in various parts of the tree. A factor to quantify the degree of accumulation is illustrated and a possible seed protective mechanism suggested

Potential Protective Role Exerted by Secoiridoids from Olea europaea L. in Cancer, Cardiovascular, Neurodegenerative, Aging-Related, and Immunoinflammatory Diseases

Iridoids, which have beneficial health properties, include a wide group of cyclopentane [c] pyran monoterpenoids present in plants and insects. The cleavage of the cyclopentane ring leads to secoiridoids. Mainly, secoiridoids have shown a variety of pharmacological effects including anti-diabetic, antioxidant, anti-inflammatory, immunosuppressive, neuroprotective, anti-cancer, and anti-obesity, which increase the interest of studying these types of bioactive compounds in depth. Secoiridoids are thoroughly distributed in several families of plants such as Oleaceae, Valerianaceae, Gentianaceae and Pedialaceae, among others. Specifically, Olea europaea L. (Oleaceae) is rich in oleuropein (OL), dimethyl-OL, and ligstroside secoiridoids, and their hydrolysis derivatives are mostly OL-aglycone, oleocanthal (OLE), oleacein (OLA), elenolate, oleoside-11-methyl ester, elenoic acid, hydroxytyrosol (HTy), and tyrosol (Ty). These compounds have proved their efficacy in the management of diabetes, cardiovascular and neurodegenerative disorders, cancer, and viral and microbial infections. Particularly, the antioxidant, anti-inflammatory, and immunomodulatory properties of secoiridoids from the olive tree (Olea europaea L. (Oleaceae)) have been suggested as a potential application in a large number of inflammatory and reactive oxygen species (ROS)-mediated diseases. Thus, the purpose of this review is to summarize recent advances in the protective role of secoiridoids derived from the olive tree (preclinical studies and clinical trials) in diseases with an important pathogenic contribution of oxidative and peroxidative stress and damage, focusing on their plausible mechanisms of the action involved.España Ministerio de Economía y Competitivida

Isolation And molecular characterization of xylella fastidiosa from different host plant species in Italy (Apulia region)

L'isolement en culture pure de la souche Xylella fastidiosa liée au syndrome de déclin rapide de l'olivier (OQDS), récemment observé dans les Pouilles (sud de l'Italie) a été tenté à partir des plantes symptomatiques naturellement infectée, principalement Olea europaea L., Polygala myrtifolia et Rosmarinus officinalis, en utilisant les méthodes d'impression et d'extraction de la sève. Avant l'isolement, RT-LAMP et qPCR ont été utilisés pour déterminer la présence de X. fastidiosa chez tous les hôtes. Des cultures bactériennes pures ont été obtenues à partir d'extraits d'Olea europaea L. et de Polygala myrtifolia étalés dans un milieu tamponné d'extrait de cystéine-levure (BCYE). Deux isolats d'olive ont ensuite été typés à l'aide du système de séquençage multilocus (MLST). Les résultats indiquent qu'Olea europaea a un grand potentiel d'isolement, et la méthode d'impression a présenté des résultats nettement meilleurs. MLST montre des points communs génétiques avec la souche De Donno (ST53) et confirme que nous avons toujours le même type de séquence ST53 dans la région.Pure culture isolation of the Xylella fastidiosa strain related to the olive quick declinesyndrome (OQDS), lately noticed in Apulia (southern Italy) was attempted fromnaturally infected symptomatic plants mainly Olea europaea L., Polygala myrtifolia and Rosmarinus officinalis using printing and sap extraction methods. Prior to isolation, RTLAMP and qPCR were used to determine the presence of Xylella fastidiosa in all hosts. Pure bacterial cultures were obtained from both Olea europaea L. and Polygala myrtifolia extracts plated in buffered cysteine-yeast extract (BCYE) media. Two olive isolates were subsequently typed using the Multilocus Sequencing System (MLST). Findings indicate that beside Olea europaea, Polygala myrtifolia seems to have a good potential for isolation, and printing method has presented significantly better results. MLST shows genetic commonality with De Donno (ST53) strain and ascertain that Apulia region still have the same sequence type ST53 in the region

Current Status of Biodiversity Assessment and Conservation of Wild Olive (Olea europaea L. subsp. europaea var. sylvestris)

Oleaster (Olea europaea L. subsp. europaea var. sylvestris) is the ancestor of cultivated olive (Olea europaea L. subsp. europaea var. europaea) and it is spread through the whole Mediterranean Basin, showing an overlapping distribution with cultivated olive trees. Climate change and new emerging diseases are expected to severely affect the cultivations of olive in the future. Oleaster presents a higher genetic variability compared to the cultivated olive and some wild trees were found adapted to particularly harsh conditions; therefore, the role of oleaster in the future of olive cultivation may be crucial. Despite the great potential, only recently the need to deeply characterize and adequately preserve the wild olive resources drew the attention of researchers. In this review, we summarized the most important morphological and genetic studies performed on oleaster trees collected in different countries of the Mediterranean Basin. Moreover, we reviewed the strategies introduced so far to preserve and manage the oleaster germplasm collections, giving a future per-spective on their role in facing the future agricultural challenges posed by climatic changes and new emerging diseases