High speed spectral domain optical coherence tomography for retinal imaging at 500,000 A‑lines per second

We present a new development of ultrahigh speed spectral domain optical coherence tomography (SDOCT) for human retinal imaging at 850 nm central wavelength by employing two high-speed line scan CMOS cameras, each running at 250 kHz. Through precisely controlling the recording and reading time periods of the two cameras, the SDOCT system realizes an imaging speed at 500,000 A-lines per second, while maintaining both high axial resolution (~8 μm) and acceptable depth ranging (~2.5 mm). With this system, we propose two scanning protocols for human retinal imaging. The first is aimed to achieve isotropic dense sampling and fast scanning speed, enabling a 3D imaging within 0.72 sec for a region covering 4x4 mm2. In this case, the B-frame rate is 700 Hz and the isotropic dense sampling is 500 A-lines along both the fast and slow axes. This scanning protocol minimizes the motion artifacts, thus making it possible to perform two directional averaging so that the signal to noise ratio of the system is enhanced while the degradation of its resolution is minimized. The second protocol is designed to scan the retina in a large field of view, in which 1200 A-lines are captured along both the fast and slow axes, covering 10 mm2, to provide overall information about the retinal status. Because of relatively long imaging time (4 seconds for a 3D scan), the motion artifact is inevitable, making it difficult to interpret the 3D data set, particularly in a way of depth-resolved en-face fundus images. To mitigate this difficulty, we propose to use the relatively high reflecting retinal pigmented epithelium layer as the reference to flatten the original 3D data set along both the fast and slow axes. We show that the proposed system delivers superb performance for human retina imaging

Master slave en-face OCT/SLO

Master Slave optical coherence tomography (MS-OCT) is an OCT method that does not require resampling of data and can be used to deliver en-face images from several depths simultaneously. As the MS-OCT method requires important computational resources, the number of multiple depth en-face images that can be produced in real-time is limited. Here, we demonstrate progress in taking advantage of the parallel processing feature of the MS-OCT technology. Harnessing the capabilities of graphics processing units (GPU)s, information from 384 depth positions is acquired in one raster with real time display of up to 40 en-face OCT images. These exhibit comparable resolution and sensitivity to the images produced using the conventional Fourier domain based method. The GPU facilitates versatile real time selection of parameters, such as the depth positions of the 40 images out of the set of 384 depth locations, as well as their axial resolution. In each updated displayed frame, in parallel with the 40 en-face OCT images, a scanning laser ophthalmoscopy (SLO) lookalike image is presented together with two B-scan OCT images oriented along rectangular directions. The thickness of the SLO lookalike image is dynamically determined by the choice of number of en-face OCT images displayed in the frame and the choice of differential axial distance between them

Fibre imaging bundles for full-field optical coherence tomography

An imaging fibre bundle is incorporated into a full-field imaging OCT system, with the aim of eliminating the mechanical scanning currently required at the probe tip in endoscopic systems. Each fibre within the imaging bundle addresses a Fizeau interferometer formed between the bundle end and the sample, a configuration which ensures down lead insensitivity of the probe fibres, preventing variations in sensitivity due to polarization changes in the many thousand constituent fibres. The technique allows acquisition of information across a planar region with single-shot measurement, in the form of a 2D image detected using a digital CCD camera. Depth scanning components are now confined within a processing interferometer external to the completely passive endoscope probe. The technique has been evaluated in our laboratory for test samples, and images acquired using the bundle-based system are presented. Data are displayed either as en-face scans, parallel to the sample surface, or as slices through the depth of the sample, with a spatial resolution of about 30 ï ­m. The minimum detectable reflectivity at present is estimated to be about 10-3, which is satisfactory for many inorganic samples. Methods of improving the signal-to- noise ratio for imaging of lower reflectivity samples are discuss

An intelligent real time 3D vision system for robotic welding tasks

MARWIN is a top-level robot control system that has been designed for automatic robot welding tasks. It extracts welding parameters and calculates robot trajectories directly from CAD models which are then verified by real-time 3D scanning and registration. MARWIN's 3D computer vision provides a user-centred robot environment in which a task is specified by the user by simply confirming and/or adjusting suggested parameters and welding sequences. The focus of this paper is on describing a mathematical formulation for fast 3D reconstruction using structured light together with the mechanical design and testing of the 3D vision system and show how such technologies can be exploited in robot welding tasks

Multidimensional en-face OCT imaging of the retina.

Fast T-scanning (transverse scanning, en-face) was used to build B-scan or C-scan optical coherence tomography (OCT) images of the retina. Several unique signature patterns of en-face (coronal) are reviewed in conjunction with associated confocal images of the fundus and B-scan OCT images. Benefits in combining T-scan OCT with confocal imaging to generate pairs of OCT and confocal images similar to those generated by scanning laser ophthalmoscopy (SLO) are discussed in comparison with the spectral OCT systems. The multichannel potential of the OCT/SLO system is demonstrated with the addition of a third hardware channel which acquires and generates indocyanine green (ICG) fluorescence images. The OCT, confocal SLO and ICG fluorescence images are simultaneously presented in a two or a three screen format. A fourth channel which displays a live mix of frames of the ICG sequence superimposed on the corresponding coronal OCT slices for immediate multidimensional comparison, is also included. OSA ISP software is employed to illustrate the synergy between the simultaneously provided perspectives. This synergy promotes interpretation of information by enhancing diagnostic comparisons and facilitates internal correction of movement artifacts within C-scan and B-scan OCT images using information provided by the SLO channel

Non-invasive imaging of subsurface paint layers with optical coherence tomography

Optical coherence tomography (OCT) systems are fast scanning infrared Michelson interferometers designed for the non-invasive examination of the interiors of the eye and subsurface structures of biological tissues. OCT has recently been applied to the non-invasive examinations of the stratigraphy of paintings and museum artefacts. So far this is the only technique capable of imaging non-invasively the subsurface structure of paintings and painted objects. Unlike the traditional method of paint cross-section examination where sampling is required, the non-invasive and non-contact nature of the technique enables the examination of the paint cross-section anywhere on a painting, as there is no longer an issue with conservation ethics regarding the taking of samples from historical artefacts. A range of applications of the technique including the imaging of stratigraphy of paintings and painted artefacts, the imaging of underdrawings to the analysis of the optical properties of paint and varnish layers is presented. Future projects on the application of OCT to art conservation are discussed