Effect of Different Carbon Concentration on Yield of Extracellular Polymeric Substances (EPS) Produced by Pseudomonas Putida ATCC 11172

The extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different carbon concentration, where Luria broth used as medium growth with and without addition of 0.5% w/v (LBG 0.5%) and 1.0% w/v (LBG 1%) glucose. The Bound EPS were extracted by ethylene diamine tetra acetic acid (EDTA) methods and precipitated by cold ethanol 3:1; while free EPS extracted only by precipitate by cold ethanol 3:1. The study shows the clear effect of carbon concentration on the production of EPS. The amount of free EPS found 120.39±2.20, 111.16±1.75 and 100.38±2.0 mg g-1 dry cell for LB, LBG 0.5 and LBG 1 respectively. Where the yield of bound EPS shows fewer amounts from free EPS, the amount of bound EPS was 29.22±1.40, 21.18±0.90 and 17.10±1.10 mg g-1 dry cell for LB, LBG 0.5 and LBG 1 respectively

Characterization of Extracellular Polymeric Substances (Eps) Produced by \u3ci\u3eCloacibacterium normanense\u3c/i\u3e Isolated from Wastewater Sludge for Sludge Settling and Dewatering

In this study, extracellular polymeric substances (EPSs) producing strain was isolated from municipal wastewater sludge (MWWS). Growth profile and the EPS production by Cloacibacterium normanense using wastewater sludge as raw material in shake flask fermentation for 96 h were investigated. The highest concentration of S-EPS (13.0 ± 0.8 g/L) and C-EPS (0.3 ± 0.1 g/L) were attained at 48 h of fermentation. S-EPS revealed higher flocculation activity (94.2%) and dewaterability (59.9%) than other types of EPS in kaolin suspension. The dewaterability of MWWS with 2 g suspended solids (SS)/L was improved by 37.6% using 0.02 ± 0.01 g/L of S-EPS and 600 mg/L of Al2(SO4)3. The study showed a promising approach of new isolated strain to produce high concentration EPS in sludge with high flocculation activity as well as good settlin

Characterization of Extracellular Polymeric Substances (Eps) Produced by \u3ci\u3eCloacibacterium normanense\u3c/i\u3e Isolated from Wastewater Sludge for Sludge Settling and Dewatering

In this study, extracellular polymeric substances (EPSs) producing strain was isolated from municipal wastewater sludge (MWWS). Growth profile and the EPS production by Cloacibacterium normanense using wastewater sludge as raw material in shake flask fermentation for 96 h were investigated. The highest concentration of S-EPS (13.0 ± 0.8 g/L) and C-EPS (0.3 ± 0.1 g/L) were attained at 48 h of fermentation. S-EPS revealed higher flocculation activity (94.2%) and dewaterability (59.9%) than other types of EPS in kaolin suspension. The dewaterability of MWWS with 2 g suspended solids (SS)/L was improved by 37.6% using 0.02 ± 0.01 g/L of S-EPS and 600 mg/L of Al2(SO4)3. The study showed a promising approach of new isolated strain to produce high concentration EPS in sludge with high flocculation activity as well as good settlin

Infeksi Biofilm Bakterial

: Biofilm is the unity of microbial cell surface surrounded by a matrix of extracellular polymeric substances (EPS). Bacteria composing the biofilm are heterogeneous in space and time. This biofilm continues to grow influenced by internal and external processes. Moreover, biofilm can be found on the surface of medical devices, as well as in bacterial endocarditis and cystic fibrosis. Biofilm that is already formed can lead to antibiotic resistance

Effect of cytostatic drug presence on extracellular polymeric substances formation in municipal wastewater treated by membrane bioreactor

The effect of the cyclophosphamide and its mean metabolites on extracellular polymeric substances (EPS) formation was investigated. Two lab-scale membrane bioreactors were followed in parallel (one with the cytostatic drugs, the second without). Chromatographic and spectroscopic studies (UV–Vis spectroscopy and IR spectroscopy) showed that the presence of CPs induced an increase in EPS concentration in the biological sludge, especially of soluble substances, mainly polysaccharides and proteins. Size exclusion chromatography analysis revealed that in the presence of CPs, macromolecular EPS were formed (polysaccharides corresponding to about 6 KDa and proteins to about 18 KDa). The formation of EPS seemed to be a protection mechanism. More important membrane fouling in reactor with CPs seemed to be related to the retention of an increased amount of soluble substances

3-D Computational Investigation of Viscoelastic Biofilms using GPUs

A biofilm is a slimy colony of bacteria and the materials they secrete, collectively called “extracellular polymeric substances (EPS)”. The EPS consists mostly of bio-polymers, which cross link into a network that behave viscoelastically under deformation. We propose a single-fluid multi-component phase field model of biofilms that captures this behavior, then use numerical simulations on GPUs to investigate the biofilm’s growth and its hydrodynamics properties

Extracellular polymeric substances facilitate the adsorption and migration of Cu 2+ and Cd 2+ in saturated porous media

Heavy metal contamination in groundwater is a serious environmental problem. Many microorganisms that survive in subsurface porous media also produce extracellular polymeric substances (EPS), but little is known about the effect of these EPS on the fate and transport of heavy metals in aquifers. In this study, EPS extracted from soil with a steam method were used to study the adsorption behaviors of Cu2+ and Cd2+, employing quartz sand as a subsurface porous medium. The results showed that EPS had a good adsorption capacity for Cu2+ (13.5 mg/g) and Cd2+ (14.1 mg/g) that can be viewed using the Temkin and Freundlich models, respectively. At a pH value of 6.5 ± 0.1 and a temperature of 20 °C, EPS showed a greater affinity for Cu2+ than for Cd2+. The binding force between EPS and quartz sand was weak. The prior saturation of the sand media with EPS solution can significantly promote the migration of the Cu2+ and Cd2+ in sand columns by 8.8% and 32.1%, respectively. When treating both metals simultaneously, the migration of Cd2+ was found to be greater than that of Cu2+. This also demonstrated that EPS can promote the co-migration of Cu2+ and Cd2+ in saturated porous media

Pengaruh Penambahan Extracellular Polymeric Substance (EPS) Sebagai Biostimulan Terhadap Laju Degradasi Lumpur IPAL PT. Indofood CBP

PT. Indofood CBP as one of the food industry that produces ingredients as its product does not miss from sludge treatment issues. The sludge inflicts troubles on the environment and PT. Indofood CBP, since there is no effective handling and processing. Extracellular Polymeric Substances (EPS) as one of alternative sludge treatment. By varying volume of the sludge: 300 gr, 400 gr, and 450 gr, and mass of the EPS: 350 gr, 400 gr, and 450 gr DW, thus it could be indicated that the more EPS are given and the less amount of sludge volume then a greater degradation of sludge. Obtained data shows the reduction of COD is 65,16% in reactor 1, 69,15% in reactor 2, 81,78% in reactor 3, 53,19% in reactor 4, 57,85% in reactor 5, 65,82% in reactor 6, 44,55% in reactor 7, 45,88% in reactor 8, 47,21% in reactor 9, 36.57% in control reactor. And the reduction of BOD is 59,29% in reactor 1, 60,62% in reactor 2, 65,04% in reactor 3, 51,77% in reactor 4, 52,65% in reactor 5, 55,75% in reactor 6, 37,61% in reactor 7, 39,82% in reactor 8, 44.69% in reactor 9, 35,84% in control reactor