New insights into muscle function during pivot feeding in seahorses

Seahorses, pipefish and their syngnathiform relatives are considered unique amongst fishes in using elastic recoil of post-cranial tendons to pivot the head extremely quickly towards small crustacean prey. It is known that pipefish activate the epaxial muscles for a considerable time before striking, at which rotations of the head and the hyoid are temporarily prevented to allow energy storage in the epaxial tendons. Here, we studied the motor control of this system in seahorses using electromyographic recordings of the epaxial muscles and the sternohyoideus-hypaxial muscles with simultaneous high-speed video recordings of prey capture. In addition we present the results from a stimulation experiment including the muscle hypothesised to be responsible for the locking and triggering of pivot feeding in seahorses (m. adductor arcus palatini). Our data confirmed that the epaxial pre-activation pattern observed previously for pipefish also occurs in seahorses. Similar to the epaxials, the sternohyoideus-hypaxial muscle complex shows prolonged anticipatory activity. Although a considerable variation in displacements of the mouth via head rotation could be observed, it could not be demonstrated that seahorses have control over strike distance. In addition, we could not identify the source of the kinematic variability in the activation patterns of the associated muscles. Finally, the stimulation experiment supported the previously hypothesized role of the m. adductor arcus palatini as the trigger in this elastic recoil system. Our results show that pre-stressing of both the head elevators and the hyoid retractors is taking place. As pre-activation of the main muscles involved in pivot feeding has now been demonstrated for both seahorses and pipefish, this is probably a generalized trait of Syngnathidae

Viral Reservoirs in Lymph Nodes of FIV-Infected Progressor and Long-Term Non-Progressor Cats during the Asymptomatic Phase.

BackgroundExamination of a cohort of cats experimentally infected with feline immunodeficiency virus (FIV) for 5.75 years revealed detectable proviral DNA in peripheral blood mononuclear cells (PBMCs) harvested during the asymptomatic phase, undetectable plasma viral RNA (FIV gag), and rarely detectable cell-associated viral RNA. Despite apparent viral latency in peripheral CD4+ T cells, circulating CD4+ T cell numbers progressively declined in progressor animals. The aim of this study was to explore this dichotomy of peripheral blood viral latency in the face of progressive immunopathology. The viral replication status, cellular immunophenotypes, and histopathologic features were compared between popliteal lymph nodes (PLNs) and peripheral blood. Also, we identified and further characterized one of the FIV-infected cats identified as a long-term non-progressor (LTNP).ResultsPLN-derived leukocytes from FIV-infected cats during the chronic asymptomatic phase demonstrated active viral gag transcription and FIV protein translation as determined by real-time RT-PCR, Western blot and in situ immunohistochemistry, whereas viral RNA in blood leukocytes was either undetectable or intermittently detectable and viral protein was not detected. Active transcription of viral RNA was detectable in PLN-derived CD4+ and CD21+ leukocytes. Replication competent provirus was reactivated ex vivo from PLN-derived leukocytes from three of four FIV-infected cats. Progressor cats showed a persistent and dramatically decreased proportion and absolute count of CD4+ T cells in blood, and a decreased proportion of CD4+ T cells in PLNs. A single long-term non-progressor (LTNP) cat persistently demonstrated an absolute peripheral blood CD4+ T cell count indistinguishable from uninfected animals, a lower proviral load in unfractionated blood and PLN leukocytes, and very low amounts of viral RNA in the PLN.ConclusionCollectively our data indicates that PLNs harbor important reservoirs of ongoing viral replication during the asymptomatic phase of infection, in spite of undetectable viral activity in peripheral blood. A thorough understanding of tissue-based lentiviral reservoirs is fundamental to medical interventions to eliminate virus or prolong the asymptomatic phase of FIV infection

The Epitheliome: agent-based modelling of the social behaviour of cells

We have developed a new computational modelling paradigm for predicting the emergent behaviour resulting from the interaction of cells in epithelial tissue. As proof-of-concept, an agent-based model, in which there is a one-to-one correspondence between biological cells and software agents, has been coupled to a simple physical model. Behaviour of the computational model is compared with the growth characteristics of epithelial cells in monolayer culture, using growth media with low and physiological calcium concentrations. Results show a qualitative fit between the growth characteristics produced by the simulation and the in vitro cell models

Elusive AGN in the XMM-Newton bright serendipitous survey

AIMS: We investigate here the nature of all the sources (35 in total) in the XBS survey (which is 86% optically identified) showing an optical spectrum dominated by the light from the host galaxy with no evidence (or little evidence) for the presence of an AGN. METHODS: We use the X-ray spectral analysis to assess the presence of an AGN in these sources and to characterize its properties. RESULTS: We detect AGN activity in 33 out of 35 sources. The remaining 2 sources are the ones with the lowest X-ray luminosity in the sample (L[2-10keV]<10^41 erg s^-1) and their X-ray emission could be produced within the host galaxy. We find that the ``recognition problem'' for AGN is very critical in the low-luminosity regime (at least 60% of the AGN with L[2-10keV]<10^43 erg s^-1 are elusive) becoming negligible for high X-ray luminosities (~1.5% of elusive AGN with L[2-10keV]>10^44 erg s^-1). This problem affects mostly absorbed AGN (~40% of type~2 AGN in the survey are elusive) but also a significant fraction of unabsorbed AGN (8%). CONCLUSIONS: We find that the simplest explanations of why these 33 (or most of them) AGNs are elusive are two: at low X-ray luminosities (<10^43 erg s^-1) the most important reason is the intrinsically low AGN/galaxy contrast (optical dilution) while at high luminosities (>10^44 erg s^-1) it is due to the optical absorption (in the Compton-thin regime, i.e. NH<10^24 cm^-2). Alternative hypotheses, like the presence of Compton-thick sources, BL Lac objects or ``non-standard'' AGN (e.g. with alpha_OX<1 or with weak/absorbed Narrow Line Region) are not supported by the data although we cannot exclude the presence in the sample of a few sources of these types.Comment: accepted for publication in A&A, 17 pages, 9 figure

Paving the way for the JWST: witnessing globular cluster formation at z>3

We report on five compact, extremely young (<10Myr) and blue (\beta_UV<-2.5, F_\lambda =\lambda^\beta) objects observed with VLT/MUSE at redshift 3.1169, 3.235, in addition to three objects at z=6.145. These sources are magnified by the Hubble Frontier Field galaxy clusters MACS~J0416 and AS1063. Their de-lensed half light radii (Re) are between 16 to 140pc, the stellar masses are ~1-20 X 10^6 Msun, the magnitudes are m_uv=28.8 - 31.4 (-17<Muv<-15) and specific star formation rates can be as large as ~800Gyr^-1. Multiple images of these systems are widely separated in the sky (up to 50'') and individually magnified by factors 3-40. Remarkably, the inferred physical properties of two objects are similar to those expected in some globular cluster formation scenarios, representing the best candidate proto-globular clusters (proto-GC) discovered so far. Rest-frame optical high dispersion spectroscopy of one of them at z=3.1169 yields a velocity dispersion \sigma_v~20km/s, implying a dynamical mass dominated by the stellar mass. Another object at z=6.145, with de-lensed Muv ~ -15.3 (m_uv ~ 31.4), shows a stellar mass and a star-formation rate surface density consistent with the values expected from popular GC formation scenarios. An additional star-forming region at z=6.145, with de-lensed m_uv ~ 32, a stellar mass of 0.5 X 10^6 Msun and a star formation rate of 0.06 Msun/yr is also identified. These objects currently represent the faintest spectroscopically confirmed star-forming systems at z>3, elusive even in the deepest blank fields. We discuss how proto-GCs might contribute to the ionization budget of the universe and augment Lya visibility during reionization. This work underlines the crucial role of JWST in characterizing the rest-frame optical and near-infrared properties of such low-luminosity high-z objects.Comment: 19 pages, 9 figures, 2 tables. MNRAS, version accepted by the refere

Seeking Quantum Speedup Through Spin Glasses: The Good, the Bad, and the Ugly

There has been considerable progress in the design and construction of quantum annealing devices. However, a conclusive detection of quantum speedup over traditional silicon-based machines remains elusive, despite multiple careful studies. In this work we outline strategies to design hard tunable benchmark instances based on insights from the study of spin glasses - the archetypal random benchmark problem for novel algorithms and optimization devices. We propose to complement head-to-head scaling studies that compare quantum annealing machines to state-of-the-art classical codes with an approach that compares the performance of different algorithms and/or computing architectures on different classes of computationally hard tunable spin-glass instances. The advantage of such an approach lies in having to only compare the performance hit felt by a given algorithm and/or architecture when the instance complexity is increased. Furthermore, we propose a methodology that might not directly translate into the detection of quantum speedup, but might elucidate whether quantum annealing has a "`quantum advantage" over corresponding classical algorithms like simulated annealing. Our results on a 496 qubit D-Wave Two quantum annealing device are compared to recently-used state-of-the-art thermal simulated annealing codes.Comment: 14 pages, 8 figures, 3 tables, way too many reference

A Systemic Receptor Network Triggered by Human cytomegalovirus Entry

Virus entry is a multistep process that triggers a variety of cellular pathways interconnecting into a complex network, yet the molecular complexity of this network remains largely unsolved. Here, by employing systems biology approach, we reveal a systemic virus-entry network initiated by human cytomegalovirus (HCMV), a widespread opportunistic pathogen. This network contains all known interactions and functional modules (i.e. groups of proteins) coordinately responding to HCMV entry. The number of both genes and functional modules activated in this network dramatically declines shortly, within 25 min post-infection. While modules annotated as receptor system, ion transport, and immune response are continuously activated during the entire process of HCMV entry, those for cell adhesion and skeletal movement are specifically activated during viral early attachment, and those for immune response during virus entry. HCMV entry requires a complex receptor network involving different cellular components, comprising not only cell surface receptors, but also pathway components in signal transduction, skeletal development, immune response, endocytosis, ion transport, macromolecule metabolism and chromatin remodeling. Interestingly, genes that function in chromatin remodeling are the most abundant in this receptor system, suggesting that global modulation of transcriptions is one of the most important events in HCMV entry. Results of in silico knock out further reveal that this entire receptor network is primarily controlled by multiple elements, such as EGFR (Epidermal Growth Factor) and SLC10A1 (sodium/bile acid cotransporter family, member 1). Thus, our results demonstrate that a complex systemic network, in which components coordinating efficiently in time and space contributes to virus entry.Comment: 26 page