190 research outputs found

    Image Perceptual Similarity Metrics for the Assessment of Basal Cell Carcinoma.

    Get PDF
    Efficient management of basal cell carcinomas (BCC) requires reliable assessments of both tumors and post-treatment scars. We aimed to estimate image similarity metrics that account for BCC's perceptual color and texture deviation from perilesional skin. In total, 176 clinical photographs of BCC were assessed by six physicians using a visual deviation scale. Internal consistency and inter-rater agreement were estimated using Cronbach's α, weighted Gwet's AC2, and quadratic Cohen's kappa. The mean visual scores were used to validate a range of similarity metrics employing different color spaces, distances, and image embeddings from a pre-trained VGG16 neural network. The calculated similarities were transformed into discrete values using ordinal logistic regression models. The Bray-Curtis distance in the YIQ color model and rectified embeddings from the 'fc6' layer minimized the mean squared error and demonstrated strong performance in representing perceptual similarities. Box plot analysis and the Wilcoxon rank-sum test were used to visualize and compare the levels of agreement, conducted on a random validation round between the two groups: 'Human-System' and 'Human-Human.' The proposed metrics were comparable in terms of internal consistency and agreement with human raters. The findings suggest that the proposed metrics offer a robust and cost-effective approach to monitoring BCC treatment outcomes in clinical settings

    Quality Enhancement of 3D Models Reconstructed By RGB-D Camera Systems

    Get PDF
    Low-cost RGB-D cameras like Microsoft\u27s Kinect capture RGB data for each vertex while reconstructing 3D models from objects with obvious drawbacks of poor mesh and texture qualities due to their hardware limitations. In this thesis we propose a combined method that enhances geometrically and chromatically 3D models reconstructed by RGB-D camera systems. Our approach utilizes Butterfly Subdivision and Surface Fitting techniques to generate smoother triangle surface meshes, where sharp features can be well preserved or minimized by different Surface Fitting algorithms. Additionally the global contrast of mesh textures is enhanced by using a modified Histogram Equalization algorithm, in which the new intensity of each vertex is obtained by applying cumulative distribution function and calculating the accumulated normalized histogram of the texture. A number of experimental results and comparisons demonstrate that our method efficiently and effectively improves the geometric and chromatic quality of 3D models reconstructed from RGB-D cameras

    Notre-Dame de Paris as a validation case to improve fire safety modelling in historic buildings

    Get PDF
    The analysis of the thermal damages in Notre-Dame de Paris is necessary to estimate the impact of the dramatic 2019 fire on the remaining structure prior to reconstruction. In doing so, the large amount of data being generated creates a benchmark environment to test the relevance of numerical fire models in the unconventional configuration of a medieval roof. While being an uncontrolled and complex configuration, it can provide insights regarding the relevance of numerical tools for fire risk assessment in historic buildings. Analysing the thermal degradation of the Lutetian limestone in a vault of the choir, experimental techniques are developed to track the in-depth maximum temperature profile reached during the fire. Numerical simulations of the fire development in the roof space then aim at replicating the observations through the evaluation of the heat flux impinging the vaults during the fire. These simulations are carried out using Fire Dynamic Simulator, which requires a large range of assumptions prior to any simulation regarding materials, geometry, meshing and scale. These assumptions are described and pave the way to a future sensitivity analysis to confront the upcoming outcomes of the simulations with the experimental observations

    Lettuce growth stage identification based on phytomorphological variations using coupled color superpixels and multifold watershed transformation

    Get PDF
    Identifying the plant's developmental growth stages from seed leaf is crucial to understand plant science and cultivation management deeply. An efficient vision-based system for plant growth monitoring entails optimum segmentation and classification algorithms. This study presents coupled color-based superpixels and multifold watershed transformation in segmenting lettuce plant from complicated background taken from smart farm aquaponic system, and machine learning models used to classify lettuce plant growth as vegetative, head development and for harvest based on phytomorphological profile. Morphological computations were employed by feature extraction of the number of leaves, biomass area and perimeter, convex area, convex hull area and perimeter, major and minor axis lengths of the major axis length the dominant leaf, and length of plant skeleton. Phytomorphological variations of biomass compactness, convexity, solidity, plant skeleton, and perimeter ratio were included as inputs of the classification network. The extracted Lab color space information from the training image set undergoes superpixels overlaying with 1,000 superpixel regions employing K-means clustering on each pixel class. Six-level watershed transformation with distance transformation and minima imposition was employed to segment the lettuce plant from other pixel objects. The accuracy of correctly classifying the vegetative, head development, and harvest growth stages are 88.89%, 86.67%, and 79.63%, respectively. The experiment shows that the test accuracy rates of machine learning models were recorded as 60% for LDA, 85% for ANN, and 88.33% for QSVM. Comparative analysis showed that QSVM bested the performance of optimized LDA and ANN in classifying lettuce growth stages. This research developed a seamless model in segmenting vegetation pixels, and predicting lettuce growth stage is essential for plant computational phenotyping and agricultural practice optimization

    Thermal signatures of skin lesions using computational thermal modeling and medical infrared imaging

    Get PDF
    Infrared (IR) thermography is a valuable quantitative diagnostic tool that allows for non-invasive, accurate measurement of skin temperature variations in the presence of a lesion. Modeling the underlying thermal and physiological processes within the body offers excellent potential for improving the thermographic measurement system design and developing more exact, quantitative assessment criteria. Using computational modeling and infrared imaging experiments, this dissertation investigates the thermal signatures of lesions of varying geometrical and physiological characteristics. We first performed a comprehensive sensitivity analysis of the computed skin temperatures in order to understand the relationships between healthy skin temperatures and the underlying thermophysical processes and tissue properties. These functional relationships provide a foundation for interpreting steady state and transient thermal signatures of skin lesions. We developed a computational thermal model for a heel deep tissue injury (DTI) to allow for an early thermographic detection and assessment capability for DTIs. The DTI models were used to develop thermographic measurement strategies and quantitative staging criteria that can be employed in a clinical setting. We analyzed the infrared images of various vascular tumors and pigmented skin lesions acquired from patient studies, using the combined white light-infrared image processing approaches. Our quantitative thermal analysis of lesions of different physiological characteristics, sizes, locations and depths will facilitate quantitative assessment and interpretation of other skin lesion thermographic images. A better understanding of the thermal behavior of skin lesions, gained using computational modeling and infrared imaging experiments in this study, can contribute to the advanced use of quantitative infrared imaging in medical diagnostic applications

    Large scale phenotyping and molecular analysis in a germplasm collection of rocket salad (Eruca vesicaria) reveal a differentiation of the gene pool by geographical origin

    Full text link
    [EN] Cultivated rocket (Eruca vesicaria) is a leafy vegetable highly appreciated for its healthpromoting virtues and consumed both raw and cooked as ready-to-use vegetable. Despite Eruca being cultivated worldwide, only a few cultivars are available and limited breeding activities have been carried out so far. Therefore, the genetic resources available represent an unexploited potential source of variation for breeding. In the present study, 155 E. vesicaria accessions from 30 countries across Europe, Asia, Africa, and America have been characterized for 54 qualitative and quantitative morphological and quality traits. Conventional descriptors and automated tools for the determination of the quality, morphology, and colour of leaves have been used. Genetic diversity was assessed using 15 inter simple sequence repeat and simple sequence repeat markers. A high level of diversity was evidenced in the collection. Significant differences were found in most of the traits with the exception of five pseudo-qualitative descriptors. The first and second dimensions of the principal components analysis with phenotypic traits accounted for 25.69% of total variation showing a stratification of the genotypes according to the European and Asian origins. In total, 75% of the variation was contained in the first 15 components having eigenvalues higher than 1.0. Also, the population structure divided the collection into two main clusters separating European genotypes from the rest. Furthermore, hierarchical cluster analysis confirmed a geographical separation, grouping the accessions into three major clusters, which were differentiated by plant architecture, leaf and flower colour, leaf water status, leaf blade shape and hairiness of the leaves and stem. Our approach has broadened the knowledge of the diversity within the Eruca gene pool, thus contributing to identify sources of variation and to select the best candidates for cultivated rocket breeding programs, as well as to determine the genetic basis of plant and leaf traits in future genome-wide association studies.The work was supported by 'RGV-FAO' project funded by the Italian Ministry of Agriculture, Food and Forestry. C. Guijarro-Real thanks the Ministerio de Educacion, Cultura y Deporte of Spain (MECD) for its financial support by means of a predoctoral FPU Grant (FPU14/06798), and for the specific grant for mobility (EST17/00354) from the same Organization.Guijarro-Real, C.; Navarro, A.; Esposito, S.; Festa, G.; Macellaro, R.; Di Cesare, C.; Fita, A.... (2020). Large scale phenotyping and molecular analysis in a germplasm collection of rocket salad (Eruca vesicaria) reveal a differentiation of the gene pool by geographical origin. Euphytica. 216(3):1-20. https://doi.org/10.1007/s10681-020-02586-xS1202163Andrey P, Maurin Y (2005) Free-D: an integrated environment for three-dimensional reconstruction from serial sections. J Neurosci Methods 145:233–244Awada L, Phillips PWB, Smyth SJ (2018) The adoption of automated phenotyping by plant breeders. Euphytica 214:148. https://doi.org/10.1007/s10681-018-2226-zBarrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust J Biol Sci 15(3):413–428Bell L, Wagstaff C (2014) Glucosinolates, myrosinase hydrolysis products, and flavonols found in rocket (Eruca sativa and Diplotaxis tenuifolia). J Agric Food Chem 62:4481–4492Bell L, Oruna Concha MJ, Wagstaff C (2015) Identification and quantification of glucosinolate and flavonol compounds in rocket salad (Eruca sativa, Eruca vesicaria and Diplotaxis tenuifolia) by LC-MS: highlighting the potential for improving nutritional value of rocket crops. Food Chem 172:852–861Bell L, Methven L, Wagstaff C (2017) The influence of phytochemical composition and resulting sensory attributes on preference for salad rocket (Eruca sativa) accessions by consumers of varying TAS2R38 diplotype. Food Chem 222:6–17Bennett RN, Mellon FA, Botting NP, Eagles J, Rosa EAS, Williamson G (2002) Identification of the major glucosinolate (4-mercaptobutyl glucosinolate) in leaves of Eruca sativa L. (salad rocket). Phytochemistry 61:25–30Bozokalfa MK, Eşiyok D, Ilbi H, Kaygisiz AT (2010) Estimates of genetic variability and association studies in quantitative plant traits of Eruca spp. landraces. Genetika 42(3):501–512Bozokalfa MK, Eşiyok D, Ilbi H, Kavak S, Kaygisiz AT (2011) Evaluation of phenotypic diversity and geographical variation of cultivated (Eruca sativa L.) and wild (Diplotaxis tenuifolia L.) rocket plant. Plant Genet Resour C 9:454–563CBI Centre for the Promotion of Imports from developing countries (2019) https://www.cbi.eu/market-information/fresh-fruit-vegetables/fresh-herbs/europe/. Accessed 21 Sept 2019Cheruiyot EK, Mumera LM, Ngetich WK, Hassanali A, Wachira F (2007) Polyhenols as potential indicators for osmotic tolerance in tea (Camellia sinensis L.). Biosci Biotechnol Biochem 71:2190–2197. https://doi.org/10.1271/bbb.70156Curtin F, Schulz P (1998) Multiple correlations and Bonferroni’s correction. Biol Psychiatr 44:775–777D’Antuono LF, Elementi S, Neri R (2008) Glucosinolates in Diplotaxis and Eruca leaves: diversity, taxonomic relations and applied aspects. Phytochemistry 69:187–199Daayf F, El Hadrami A, El-Bebany AE, Henriquez MA, Yao Z, Derksen H, El-Hadrami I, Adam LR (2012) Phenolic compounds in plant defense and pathogen counter-defense mechanisms. Rec Adv Polyphen Res 3:191–208Dalin P, Agren J, Björkman C, Huttumen P, Kärkkäinen K (2008) Leaf trichome formation and plant resistance to herbivory. In: Schaller A (ed) Induced plant resistance to herbivory. Springer, Berlin, pp 89–105Earl DA, vonHoldt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361Egea-Gilabert C, Fernandez JA, Migliaro D, Martinez Sanchez JJ, Vicente MJ (2009) Genetic variability in wild vs. cultivated Eruca vesicaria populations as assessed by morphological, agronomical and molecular analyses. Sci Hortic 121:260–266Fine PVA (2015) Ecological and evolutionary drivers of geographic variation in species diversity. Annu Rev Ecol Evol Syst 46:369–392. https://doi.org/10.1146/annurev-ecolsys-112414-054102Gilardi G, Chen G, Garibaldi A, Zhiping C, Gullino ML (2007) Resistance of different rocket cultivars to wilt caused by strains of Fusarium oxysporum under artificial inoculation conditions. J Plant Pathol 89:113–117Gómez-Campo C (2003) Morphological characterization of Eruca vesicaria (Cruciferae) germplasm. Bocconea 16:615–624Guijarro-Real C, Prohens J, Rodriguez-Burruezo A, Adalid-Martínez AM, López-Gresa MP, Fita A (2019) Wild edible fool’s watercress: a potential crop with high nutraceutical properties. PeerJ 7:e6296Hall MKD, Jobling JJ, Rogers GS (2012) Some perspectives on rocket as a vegetable crop: a review. Veg Crop Res Bull 76:21–41Hanldey R, Ekbom B, Ågren J (2005) Variation in trichome density and resistance against a specialist insect herbivore in natural populations of Arabidopsis thaliana. Ecol Entomol 30:284–292Hauser MT (2014) Molecular basis of natural variation and environmental control of trichome patterning. Front Plant Sci 5:320Higdon JV, Delage B, Williams DE, Dashwood RH (2007) Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol Res 55:224–236IPGRI (1999) Descriptors for rocket (Eruca spp.). International Plant Genetic Resources Institute, RomeLenzi A, Tesi R (2000) Effect of some cultural factors on nitrate accumulation in rocket [Diplotaxis tenuifolia (L.) D.C. – Eruca sativa Mill.]. Riv Agronomia 34(4):419–424Mantel NA (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220Meyer RS, Purugganan MD (2013) Evolution of crop species: genetics of domestication and diversification. Nat Rev Genet 14:840–852Ninfali P, Mea G, Giorgini S, Rocchi M, Bacchiocca M (2005) Antioxidant capacity of vegetables spices and dressings relevant to nutrition. Br J Nutr 93:257–266Padulosi S, Pignone D (1996) Rocket: a mediterranean crop for the world. Report of a workshop. IPGRI International Plant Genetic Resources Institute, Bioversity International, Rome, p 101Pane C, Sigillo L, Caputo M, Serratore G, Zaccardelli M, Tripodi P (2017) Response of rocket salad germplasm (Eruca and Diplotaxis spp.) to major pathogens causing damping-off, wilting and leaf spot diseases. Arch Phytopathol Plant Protect 50:167–177Pariyar S, Eichert T, Goldbach HE, Hunsche M, Burkhardt J (2013) The exclusion of ambient aerosols changes the water relations of sunflower (Helianthus annuus) and bean (Vicia vaba) plants. Environ Exp Bot 88:43–52Pasini F, Verardo V, Caboni MF, D’Antuono LF (2012) Determination of glucosinolates and phenolic compounds in rocket salad by HPLC-DAD−MS: evaluation of Eruca sativa Mill. and Diplotaxis tenuifolia L. genetic resources. Food Chem 133:1025–1033Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The utility of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:225–238Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98:107–112Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959Singleton VL, Rossi JA Jr (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16:144–158Taiz L, Zeiger E (2006) Plant physiology, 4th edn. Sinauer Associates Inc., SunderlandTanentzap FM, Stempel A, Ryser P (2015) Reliability of leaf relative water content (RWC) measurements after storage: consequences for in situ measurements. Botany 93:535–541Taranto F, Francese G, Di Dato F, D’Alessandro A, Greco B, Onofaro Sanajà V, Pentangelo A, Mennella G, Tripodi P (2016) Leaf metabolic, genetic, and morphophysiological profiles of cultivated and wild rocket salad (Eruca and Diplotaxis spp.). J Agric Food Chem 64:5824–5836Thakur AK, Singh KH, Singh L, Nanjundan J, Khan YJ, Singh D (2018) SSR marker variations in Brassica species provide insight into the origin and evolution of Brassica amphidiploids. Hereditas 155:6. https://doi.org/10.1186/s41065-017-0041-5The Plant List (2019). http://www.theplantlist.org/tpl1.1/search?q=Eruca. Accessed 21 Sept 2019Tripodi P, Francese G, Mennella G (2017) Rocket salad: crop description, bioactive compounds and breeding perspectives. Adv Hortic Sci. https://doi.org/10.13128/ahs-21087Vavilov NI (1926) Centers of origin of cultivated plants. Bull Appl Bot Genet Plant Breed 16:1–248Wagner GJ (1991) Secreting glandular trichomes: more than just hairs. Plant Physiol 96:675–679Warwick SI, Gukel RK, Gomez-Campo C, James T (2007) Genetic variation in Eruca vesicaria (L.) Cav. Plant Genet Resour C 5:142–15

    THE EFFECTS WILD FIRES HAVE ON SKELETAL REMAINS

    Get PDF

    Following Darwin's footsteps using 'the most wonderful plants in the world': the ecophysiological responses of the carnivorous plant Drosera rotundifolia to nitrogen availability.

    Get PDF
    Nitrogen (N) is an essential element to plants for growth, maintenance and reproduction, however most N does not exist in a form that is biologically available to plants. In order to maximise the acquisition and retention of N, plants have evolved a variety of morphological and physiological adaptations and life history strategies, as well as the ability to respond plastically to changes in resource availability in ecological time. Determining the ecophysiological responses of plants to changes in root N availability is crucial to further understanding of the mechanisms underlying competitive interactions between plants, and between plants and other organisms, that ultimately contribute to community structure and ecosystem functioning. Carnivorous plants are ideal systems for investigating ecophysiological responses to N availability as:- (i) they share a unique adaptation for obtaining supplemental N from captured prey, therefore ecological stoichiometry and energetic cost/benefit models may be explored; (ii) the trait of botanical carnivory is widely considered to have independently co-evolved as a response to N-deficient, sunny and wet environments, therefore resource allocation trade-offs between plant investment in N and carbon (C) acquisition may be observed, and (iii) they are extremely sensitive to changes in root N availability in ecological time. In this research, the carnivorous plant Drosera rotundifolia (round-leaved sundew) was used to address several unanswered ecophysiological and evolutionary questions relating to patterns and processes of prey capture and the N nutrition of carnivorous plants. Furthermore, the potential for reducing uncertainty in the calculation of plant reliance on carnivory using a δ15N natural abundance multi-level linear mixing model was explored. A combined approach of in-situ and ex-situ studies was employed, using co-occurring non-carnivorous plants or carnivorous plant species with differing evolutionary lineages or prey capture mechanisms respectively to provide context. Results show that the adaptations of carnivory, high reproductive investment and a relatively short life span enable Drosera rotundifolia to survive and thrive in an extreme, N deficient environment. Phenotypically plastic responses by the plant to light and root N availability provide evidence of resource allocation trade-offs between investment in carnivory for N acquisition and in photosynthesis for C acquisition. Plants invested less heavily in prey capture (measured as the stickiness of leaf mucilage) as N availability increased or light availability decreased. These results show that the energetic costs associated with carnivory are avoided by the plant when less costly sources of N are available for uptake and that the production of carbon-rich mucilage is only made under nutrient-limited and well-lit conditions. Results obtained from the comparison of captured insect prey with background invertebrates of potential prey indicate that Drosera rotundifolia is a dietary generalist, where the quantity of prey captured per plant is positively correlated with leaf stickiness and total leaf area. Plant reliance on prey-derived N decreased with increasing root N availability, providing evidence that carnivory is only of net benefit to the plant in N-deficient and well-lit environments, as the photosynthetic costs of investment in the trait are not exceeded by the energetic gain from prey N uptake in shady or dry habitats. A more accurate and precise method for calculating plant reliance on botanical carnivory is presented which incorporates the insect diet of the plant. This method has wider significance for reducing uncertainty in the calculation of relative source contributions to a mixture for most natural abundance applications using a multi-level linear mixing model. To conclude, results from this research further understanding of the ecophysiological mechanisms underlying plant responses to changes in resource availability and the selective pressures driving the evolution of plant adaptations. These results therefore assist with predicting how plants and plant communities may respond to sustained N deposition inputs and future environmental scenarios

    Microbial Diversity as a Bioindicator of the Impact of Fires

    Get PDF
    Wildfires are a major problem in Galicia and frequency and severity is increasing due to climate change. Prescribed burning is widely used as a management tool to prevent high severity fire but information about the effect of repetitive burning on soil quality is scarce. The effectiveness of post-fire rehabilitation techniques on reducing soil erosion is evident, but its effect on soil properties is unknown. The aim of this study is to assess the impact of fires on soil, considering fire severity and recurrence, and to evaluate the use of microbial parameters as indicators of these changes. Moreover, it aims to analyse the effect of straw mulching and seeding on soil quality and vegetation recovery
    • …
    corecore