High-Resolution Vertical Habitat Mapping of a Deep-Sea Cliff offshore Greenland

Recent advances in deep-sea exploration with underwater vehicles have led to the discovery of vertical environments inhabited by a diverse sessile fauna. However, despite their ecological importance, vertical habitats remain poorly characterized by conventional downward-looking survey techniques. Here we present a high-resolution 3-dimensional habitat map of a vertical cliff hosting a suspension-feeding community at the flank of an underwater glacial trough in the Greenland waters of the Labrador Sea. Using a forward-looking set-up on a Remotely Operated Vehicle (ROV), a high-resolution multibeam echosounder was used to map out the topography of the deep-sea terrain, including, for the first time, the backscatter intensity. Navigational accuracy was improved through a combination of the USBL and the DVL navigation of the ROV. Multi-scale terrain descriptors were derived and assigned to the 3D point cloud of the terrain. Following an unsupervised habitat mapping approach, the application of a K-means clustering revealed four potential habitat types, driven by geomorphology, backscatter and fine-scale features. Using groundtruthing seabed images, the ecological significance of the four habitat clusters was assessed in order to evaluate the benefit of unsupervised habitat mapping for further fine-scale ecological studies of vertical environments. This study demonstrates the importance of a priori knowledge of the terrain around habitats that are rarely explored for ecological investigations. It also emphasizes the importance of remote characterization of habitat distribution for assessing the representativeness of benthic faunal studies often constrained by time-limited sampling activities. This case study further identifies current limitations (e.g., navigation accuracy, irregular terrain acquisition difficulties) that can potentially limit the use of deep-sea terrain models for fine-scale investigations

Benthic habitat mapping in coastal waters of south–east Australia

The Victorian Marine Mapping Project will improve knowledge on the location, spatial distribution, condition and extent of marine habitats and associated biodiversity in Victorian State waters. This information will guide informed decision making, enable priority setting, and assist in targeted natural resource management planning. This project entails benthic habitat mapping over 500 square kilometers of Victorian State waters using multibeam sonar, towed video and image classification techniques. Information collected includes seafloor topography, seafloor softness and hardness (reflectivity), and information on geology and benthic flora and fauna assemblages collectively comprising habitat. Computerized semi-automated classification techniques are also being developed to provide a cost effective approach to rapid mapping and assessment of coastal habitats.Habitat mapping is important for understanding and communicating the distribution of natural values within the marine environment. The coastal fringe of Victoria encompasses a rich and diverse ecosystem representative of coastal waters of South-east Australia. To date, extensive knowledge of these systems is limited due to the lack of available data. Knowledge of the distribution and extent of habitat is required to target management activities most effectively, and provide the basis to monitor and report on their status in the future.<br /

Core-scale geophysical and hydromechanical analysis of seabed sediments affected by CO2 venting

Safe offshore Carbon Capture Utilization and Storage (CCUS) includes monitoring of the subseafloor, to identify and assess potential CO2 leaks from the geological reservoir through seal bypass structures. We simulated CO2-leaking through shallow marine sediments of the North Sea, using two gravity core samples from ∼1 and ∼2.1 m below seafloor. Both samples were subjected to brine−CO2 flow-through, with continuous monitoring of their transport, elastic and mechanical properties, using electrical resistivity, permeability, P-wave velocity and attenuation, and axial strains. We used the collected geophysical data to calibrate a resistivity-saturation model based on Archie’s law extended for clay content, and a rock physics for the elastic properties. The P-wave attributes detected the presence of CO2 in the sediment, but failed in providing accurate estimates of the CO2 saturation. Our results estimate porosities of 0.44 and 0.54, a background permeability of ∼10−15 and ∼10-17 m2, and maximum CO2 saturation of 18 % and 10 % (±5 %), for the sandier (shallower) and muddier (deeper) sample, respectively. The finer-grained sample likely suffered some degree of gas-induced fracturing, exhibiting an effective CO2 permeability increase sharper than the coarser-grained sample. Our core-scale multidisciplinary experiment contributes to improve the general interpretation of shallow sub-seafloor gas distribution and migration patterns

A new methodology for quantifying bubble flow rates in deep water using splitbeam echosounders: Examples from the Arctic offshore NW-Svalbard

Quantifying marine methane fluxes of free gas (bubbles) from the seafloor into the water column is of importance for climate related studies, for example, in the Arctic, reliable methodologies are also of interest for studying man-made gas and oil leakage systems at hydrocarbon production sites. Hydroacoustic surveys with singlebeam and nowadays also multibeam systems have been proven to be a successful approach to detect bubble release from the seabed. A number of publications used singlebeam echosounder data to indirectly quantify free gas fluxes via empirical correlations between gas fluxes observed at the seafloor and the hydroacoustic response. Others utilize the hydroacoustic information in an inverse modeling approach to derive bubble fluxes. Here, we present an advanced methodology using data from splitbeam echosounder systems for analyzing gas release water depth (> 100m). We introduce a new MATLAB-based software for processing and interactively editing data and we present how bubble-size distribution, bubble rising speed and the model used for calculating the backscatter response of single bubbles influence the final gas flow rate calculations. As a result, we highlight the need for further investigations on how large, wobbly bubbles, bubble clouds, and multi-scattering influence target strength. The results emphasize that detailed studies of bubble-size distributions and rising speeds need to be performed in parallel to hydroacoustic surveys to achieve realistic mediated methane flow rate and flux quantifications

Implementing a reference backscatter calibration technique on a multi-sector multibeam echosounder

Increasingly, national hydrographic agencies are committing to routine acquisition of seabed backscatter strength estimates from multibeam echosounders (MBES) as part of national programs for seabed characterization. As part of their bathymetric survey mandate, these agencies have a long history of sounding quality control utilizing absolute and relative calibration (reference surfaces and crossover comparisons). Equivalent quality control is, however, not yet in place for managing seabed backscatter strength measurements, as the majority of the collected data is not absolutely referenced.Herein, a new technique for cross-calibrating a MBES with a reference calibrated split beam echosounder (SBES) was implemented. Broadband reference bottom backscatter strength (45-450 kHz) from areas with different seafloor types, derived from data obtained with Simrad EK80 SBES, is used to adjust the received acoustic intensities acquired from the same areas with several multi-sector MBES (Kongsberg Maritime EM2040P, EM710 and EM712), thereby enabling the routine collection of absolutely referenced bottom backscatter strength measurements. Previous efforts to implement a similar cross-calibration only considered a simplified vertically referenced ensonification geometry, ignoring the dynamic variations due to vessel rotations and active stabilization. As a result, neither the rotation of the beam pattern with respect to the vertical reference nor the compensation due to active beam stabilization were accounted for. Furthermore, this method properly accounts for modern MBES which have multiple transmit sectors over multiple swaths with the associated changes in frequency and signal modulation. The main output of this research is a set of two-dimensional arrays of correctors, derived for each transmit sector - the correction heatmap - providing estimates of the necessary calibration, as a function of across- and along-track sonar referenced angles. To test the repeatability of the proposed technique, correction heatmaps derived for the same system (using the same settings), but with data from different reference areas, were compared, resulting in differences generally within ± ~2 dB. Finally, a pre-calibrated MBES was used to survey a different location and establish a reference area, enabling the subsequent calibration of sonars that use the same frequencies

High-Resolution Vertical Habitat Mapping of a Deep-Sea Cliff Offshore Greenland

Recent advances in deep-sea exploration with underwater vehicles have led to the discovery of vertical environments inhabited by a diverse sessile fauna. However, despite their ecological importance, vertical habitats remain poorly characterized by conventional downward-looking survey techniques. Here we present a high-resolution 3-dimensional habitat map of a vertical cliff hosting a suspension-feeding community at the flank of an underwater glacial trough in the Greenland waters of the Labrador Sea. Using a forward-looking set-up on a Remotely Operated Vehicle (ROV), a high-resolution multibeam echosounder was used to map out the topography of the deep-sea terrain, including, for the first time, the backscatter intensity. Navigational accuracy was improved through a combination of the USBL and the DVL navigation of the ROV. Multi-scale terrain descriptors were derived and assigned to the 3D point cloud of the terrain. Following an unsupervised habitat mapping approach, the application of a K-means clustering revealed four potential habitat types, driven by geomorphology, backscatter and fine-scale features. Using groundtruthing seabed images, the ecological significance of the four habitat clusters was assessed in order to evaluate the benefit of unsupervised habitat mapping for further fine-scale ecological studies of vertical environments. This study demonstrates the importance of a priori knowledge of the terrain around habitats that are rarely explored for ecological investigations. It also emphasizes the importance of remote characterization of habitat distribution for assessing the representativeness of benthic faunal studies often constrained by time-limited sampling activities. This case study further identifies current limitations (e.g., navigation accuracy, irregular terrain acquisition difficulties) that can potentially limit the use of deep-sea terrain models for fine-scale investigations

Seafloor change detection using multibeam echosounder backscatter: case study on the Belgian part of the North Sea

To characterize seafloor substrate type, seabed mapping and particularly multibeam echosounding are increasingly used. Yet, the utilisation of repetitive MBES-borne backscatter surveys to monitor the environmental status of the seafloor remains limited. Often methodological frameworks are missing, and should comprise of a suite of change detection procedures, similarly to those developed in the terrestrial sciences. In this study, pre-, ensemble and post-classification approaches were tested on an eight km2 study site within a Habitat Directive Area in the Belgian part of the North Sea. In this area, gravel beds with epifaunal assemblages were observed. Flourishing of the fauna is constrained by overtopping with sand or increased turbidity levels, which could result from anthropogenic activities. Monitoring of the gravel to sand ratio was hence put forward as an indicator of good environmental status. Seven acoustic surveys were undertaken from 2004 to 2015. The methods allowed quantifying temporal trends and patterns of change of the main substrate classes identified in the study area; namely fine to medium homogenous sand, medium sand with bioclastic detritus and medium to coarse sand with gravel. Results indicated that by considering the entire study area and the entire time series, the gravel to sand ratio fluctuated, but was overall stable. Nonetheless, when only the biodiversity hotspots were considered, net losses and a gradual trend, indicative of potential smothering, was captured by ensemble and post-classification approaches respectively. Additionally, a two-dimensional morphological analysis, based on the bathymetric data, suggested a loss of profile complexity from 2004 to 2015. Causal relationships with natural and anthropogenic stressors are yet to be established. The methodologies presented and discussed are repeatable and can be applied to broad-scale geographical extents given that broad-scale time series datasets become available

The cold-water coral province of the eastern Ligurian Sea (NW Mediterranean Sea): historical and novel evidences

Several bathyal cold-water coral provinces, characterized by a lush growth of habitat-forming scleractinians, have been recognized in the Mediterranean Sea. However, the search for this biogenic habitat only marginally targeted the Italian coast of the Ligurian Sea (NW Mediterranean basin) despite historical and a few recent local studies in the region reporting the presence of corals. This study used bathymetry maps, side-scan sonar profiles, historical charts, and trawling routes to identify sites that could potentially host coral habitats in the eastern sector of the Ligurian Sea. Remotely operated vehicle video footage from various projects (2015-2021, 20 dives) was then used to characterize four sub-areas (Genoa Plateau, Portofino, Deiva Marina, and Monterosso) where corals were detected between 450 m and 750 m depth. Radiocarbon dating was used to trace back the geological history of the coral structures. A small coral mound, impacted by trawling activities, was found on the Genoa Plateau, while four massive coral structures were found in the other sub-areas, mainly located in a morphologically complex and highly energetic canyon region. High levels of megafaunal biodiversity, including rarely reported alcyonaceans as Placogorgia coronata, were observed together with moderate fishing impact. Overall, the identified coral areas potentially account for 9 km(2) of both subfossil mounds (as old as 13300 years BP), dominated by Desmophyllum pertusum, and living reefs, dominated by Madrepora oculata, the latter representing up to 23% of the substrate coverage. The few living colonies of D. pertusum in the area represent the first documented records for the Ligurian Sea. These data support the presence of a distinct eastern Ligurian cold-water coral province

Deep-reef fish communities of the Great Barrier Reef shelf-break: trophic structure and habitat associations

The ecology of habitats along the Great Barrier Reef (GBR) shelf-break has rarely been investigated. Thus, there is little understanding of how associated fishes interact with deeper environments. We examined relationships between deep-reef fish communities and benthic habitat structure. We sampled 48 sites over a large depth gradient (54–260 m) in the central GBR using Baited Remote Underwater Video Stations and multibeam sonar. Fish community composition differed both among multiple shelf-break reefs and habitats within reefs. Epibenthic cover decreased with depth. Deep epibenthic cover included sponges, corals, and macro-algae, with macro-algae present to 194 m. Structural complexity decreased with depth, with more calcified reef, boulders, and bedrock in shallower depths. Deeper sites were flatter and more homogeneous with softer substratum. Habitats were variable within depth strata and were reflected in different fish assemblages among sites and among locations. Overall, fish trophic groups changed with depth and included generalist and benthic carnivores, piscivores, and planktivores while herbivores were rare below 50 m. While depth influenced where trophic groups occurred, site orientation and habitat morphology determined the composition of trophic groups within depths. Future conservation strategies will need to consider the vulnerability of taxa with narrow distributions and habitat requirements in unique shelf-break environments

ESTIMATION OF MEASUREMENT UNCERTAINTY OF SEAFLOOR ACOUSTIC BACKSCATTER

In the last three decades, Multibeam echo sounders (MBES) have become the tool of choice to study the seafloor. MBES collects two distinct types of data: bathymetry that provides topographic details of the seafloor and backscatter that has the potential to characterize the seafloor. While the uncertainty associated with MBE bathymetry has been well studied, the uncertainty in MBES backscatter measurement has received relatively little attention, hindering the improvements in quantitative analysis of backscatter data. Both acquisition and processing stages can introduce uncertainty in the final seafloor backscatter products. Application of well-established uncertainty quantification principles to seafloor backscatter data is challenging for several reasons: the uncertainty sources are not well known, they vary on a case-by-case basis, and standards do not exist for acquisition and processing. This dissertation focuses on assessing uncertainty in backscatter measurements and is comprised of four separate but related studies that identify and address the challenges of uncertainty quantification of backscatter measurements. The first study (Lucieer et al., 2018) which is presented as background, describes an end users’ survey identifying key uses and challenges of backscatter data acquisition and processing. The study identified that consistency and repeatability of backscatter measurements is a major constraint in the use and re-use of backscatter. The second study (Malik et al., 2018), identified the sources of uncertainty and categorized them as significant or insignificant based on various use cases. The most significant sources of uncertainty were found to be inherent statistical fluctuations in the backscatter measurement, calibration uncertainty, seafloor slope and water column absorption estimation. While calibration uncertainty remains the main issue in advancing the quantitative use of the backscatter, the other sources were also shown to cause large uncertainties. These include non-standardized methods used to account for seafloor slope and absorption, and data interpretation errors due to missing background information about the processing procedures. With a comprehensive list of uncertainty sources established, two uncertainty sources, seafloor slope and processing errors, were examined further in the third (Malik, 2019) and the fourth (Malik et al., submitted) study respectively. Seafloor slope corrections are important to correct for both the area insonified and the incidence angle. Both of these corrections are adversely affected if seafloor slope corrections are not applied. Even in cases where the seafloor slope is used, further uncertainty can occur if the highest resolution bathymetry is not used. The results from this study showed that for the purpose of accurate slope corrections, the spatial scale of backscatter data should be selected based on the best available bathymetry. The majority of end users depend on third-party software solutions to process the backscatter data. The fourth study evaluated the output of three commonly used software packages after inputting the same data set and found that there were significant differences in the outputs. This issue was addressed by working closely with software developers to explore options to make the processing chain more transparent. Two intermediate processing stages were proposed and implemented in three commonly used software tools. However, due to proprietary restrictions, it was not possible to know the full details of the software processing packages. Differing outputs likely result, in part, from the different approaches used by the various software packages to read the raw data. Quality assessment and uncertainty quantification of MBES backscatter measurements is still at an early stage and further work is required to develop data acquisition and processing standards to improve consistency in the backscatter acquisition and processing. Publications: Lucieer, V.; Roche, M.; Degrendele, K.; Malik, M.; Dolan, M.; Lamarche, G. User expectations for multibeam echo sounders backscatter strength data-looking back into the future. Mar. Geophys. Res. 2018, 39, 23–40. doi:10.1007/s11001-017-9316-5. Malik, M.; Lurton, X.; Mayer, L. A framework to quantify uncertainties of seafloor backscatter from swath mapping echosounders. Mar. Geophys. Res. 2018, 39, 151–168. doi.org/10.1007/s11001-018-9346-7. Malik, M. Sources and Impacts of Bottom Slope Uncertainty on Estimation of Seafloor Backscatter from Swath Sonars. Geosciences 2019, 9, 183. doi: 10.3390/geosciences9040183. Malik, M.; Schimel, A.; Masetti, G.; Roche, M.; Deunf, J.L.; Dolan, M.; Beaudoin, J.; Augustin, J.M.; Hamilton, T.; Parnum, I. Results from the first phase of the Backscatter Software Inter-comparison Project. Geosciences. Submitted