Protein Pattern Formation

Protein pattern formation is essential for the spatial organization of many intracellular processes like cell division, flagellum positioning, and chemotaxis. A prominent example of intracellular patterns are the oscillatory pole-to-pole oscillations of Min proteins in \textit{E. coli} whose biological function is to ensure precise cell division. Cell polarization, a prerequisite for processes such as stem cell differentiation and cell polarity in yeast, is also mediated by a diffusion-reaction process. More generally, these functional modules of cells serve as model systems for self-organization, one of the core principles of life. Under which conditions spatio-temporal patterns emerge, and how these patterns are regulated by biochemical and geometrical factors are major aspects of current research. Here we review recent theoretical and experimental advances in the field of intracellular pattern formation, focusing on general design principles and fundamental physical mechanisms.Comment: 17 pages, 14 figures, review articl

Engineering simulations for cancer systems biology

Computer simulation can be used to inform in vivo and in vitro experimentation, enabling rapid, low-cost hypothesis generation and directing experimental design in order to test those hypotheses. In this way, in silico models become a scientific instrument for investigation, and so should be developed to high standards, be carefully calibrated and their findings presented in such that they may be reproduced. Here, we outline a framework that supports developing simulations as scientific instruments, and we select cancer systems biology as an exemplar domain, with a particular focus on cellular signalling models. We consider the challenges of lack of data, incomplete knowledge and modelling in the context of a rapidly changing knowledge base. Our framework comprises a process to clearly separate scientific and engineering concerns in model and simulation development, and an argumentation approach to documenting models for rigorous way of recording assumptions and knowledge gaps. We propose interactive, dynamic visualisation tools to enable the biological community to interact with cellular signalling models directly for experimental design. There is a mismatch in scale between these cellular models and tissue structures that are affected by tumours, and bridging this gap requires substantial computational resource. We present concurrent programming as a technology to link scales without losing important details through model simplification. We discuss the value of combining this technology, interactive visualisation, argumentation and model separation to support development of multi-scale models that represent biologically plausible cells arranged in biologically plausible structures that model cell behaviour, interactions and response to therapeutic interventions

Smartphone picture organization: a hierarchical approach

We live in a society where the large majority of the population has a camera-equipped smartphone. In addition, hard drives and cloud storage are getting cheaper and cheaper, leading to a tremendous growth in stored personal photos. Unlike photo collections captured by a digital camera, which typically are pre-processed by the user who organizes them into event-related folders, smartphone pictures are automatically stored in the cloud. As a consequence, photo collections captured by a smartphone are highly unstructured and because smartphones are ubiquitous, they present a larger variability compared to pictures captured by a digital camera. To solve the need of organizing large smartphone photo collections automatically, we propose here a new methodology for hierarchical photo organization into topics and topic-related categories. Our approach successfully estimates latent topics in the pictures by applying probabilistic Latent Semantic Analysis, and automatically assigns a name to each topic by relying on a lexical database. Topic-related categories are then estimated by using a set of topic-specific Convolutional Neuronal Networks. To validate our approach, we ensemble and make public a large dataset of more than 8,000 smartphone pictures from 40 persons. Experimental results demonstrate major user satisfaction with respect to state of the art solutions in terms of organization.Peer ReviewedPreprin

Data modelling for emergency response

Emergency response is one of the most demanding phases in disaster management. The fire brigade, paramedics, police and municipality are the organisations involved in the first response to the incident. They coordinate their work based on welldefined policies and procedures, but they also need the most complete and up-todate information about the incident, which would allow a reliable decision-making.\ud There is a variety of systems answering the needs of different emergency responders, but they have many drawbacks: the systems are developed for a specific sector; it is difficult to exchange information between systems; the systems offer too much or little information, etc. Several systems have been developed to share information during emergencies but usually they maintain the nformation that is coming from field operations in an unstructured way.\ud This report presents a data model for organisation of dynamic data (operational and situational data) for emergency response. The model is developed within the RGI-239 project ‘Geographical Data Infrastructure for Disaster Management’ (GDI4DM)

Graph-based Facial Affect Analysis: A Review of Methods, Applications and Challenges

Facial affect analysis (FAA) using visual signals is important in human-computer interaction. Early methods focus on extracting appearance and geometry features associated with human affects, while ignoring the latent semantic information among individual facial changes, leading to limited performance and generalization. Recent work attempts to establish a graph-based representation to model these semantic relationships and develop frameworks to leverage them for various FAA tasks. In this paper, we provide a comprehensive review of graph-based FAA, including the evolution of algorithms and their applications. First, the FAA background knowledge is introduced, especially on the role of the graph. We then discuss approaches that are widely used for graph-based affective representation in literature and show a trend towards graph construction. For the relational reasoning in graph-based FAA, existing studies are categorized according to their usage of traditional methods or deep models, with a special emphasis on the latest graph neural networks. Performance comparisons of the state-of-the-art graph-based FAA methods are also summarized. Finally, we discuss the challenges and potential directions. As far as we know, this is the first survey of graph-based FAA methods. Our findings can serve as a reference for future research in this field.Comment: 20 pages, 12 figures, 5 table

Efficient processing of large-scale spatio-temporal data

Millionen Geräte, wie z.B. Mobiltelefone, Autos und Umweltsensoren senden ihre Positionen zusammen mit einem Zeitstempel und weiteren Nutzdaten an einen Server zu verschiedenen Analysezwecken. Die Positionsinformationen und übertragenen Ereignisinformationen werden als Punkte oder Polygone dargestellt. Eine weitere Art räumlicher Daten sind Rasterdaten, die zum Beispiel von Kameras und Sensoren produziert werden. Diese großen räumlich-zeitlichen Datenmengen können nur auf skalierbaren Plattformen wie Hadoop und Apache Spark verarbeitet werden, die jedoch z.B. die Nachbarschaftsinformation nicht ausnutzen können - was die Ausführung bestimmter Anfragen praktisch unmöglich macht. Die wiederholten Ausführungen der Analyseprogramme während ihrer Entwicklung und durch verschiedene Nutzer resultieren in langen Ausführungszeiten und hohen Kosten für gemietete Ressourcen, die durch die Wiederverwendung von Zwischenergebnissen reduziert werden können. Diese Arbeit beschäftigt sich mit den beiden oben beschriebenen Herausforderungen. Wir präsentieren zunächst das STARK Framework für die Verarbeitung räumlich-zeitlicher Vektor- und Rasterdaten in Apache Spark. Wir identifizieren verschiedene Algorithmen für Operatoren und analysieren, wie diese von den Eigenschaften der zugrundeliegenden Plattform profitieren können. Weiterhin wird untersucht, wie Indexe in der verteilten und parallelen Umgebung realisiert werden können. Außerdem vergleichen wir Partitionierungsmethoden, die unterschiedlich gut mit ungleichmäßiger Datenverteilung und der Größe der Datenmenge umgehen können und präsentieren einen Ansatz um die auf Operatorebene zu verarbeitende Datenmenge frühzeitig zu reduzieren. Um die Ausführungszeit von Programmen zu verkürzen, stellen wir einen Ansatz zur transparenten Materialisierung von Zwischenergebnissen vor. Dieser Ansatz benutzt ein Entscheidungsmodell, welches auf den tatsächlichen Operatorkosten basiert. In der Evaluierung vergleichen wir die verschiedenen Implementierungs- sowie Konfigurationsmöglichkeiten in STARK und identifizieren Szenarien wann Partitionierung und Indexierung eingesetzt werden sollten. Außerdem vergleichen wir STARK mit verwandten Systemen. Im zweiten Teil der Evaluierung zeigen wir, dass die transparente Wiederverwendung der materialisierten Zwischenergebnisse die Ausführungszeit der Programme signifikant verringern kann.Millions of location-aware devices, such as mobile phones, cars, and environmental sensors constantly report their positions often in combination with a timestamp to a server for different kinds of analyses. While the location information of the devices and reported events is represented as points and polygons, raster data is another type of spatial data, which is for example produced by cameras and sensors. This Big spatio-temporal Data needs to be processed on scalable platforms, such as Hadoop and Apache Spark, which, however, are unaware of, e.g., spatial neighborhood, what makes them practically impossible to use for this kind of data. The repeated executions of the programs during development and by different users result in long execution times and potentially high costs in rented clusters, which can be reduced by reusing commonly computed intermediate results. Within this thesis, we tackle the two challenges described above. First, we present the STARK framework for processing spatio-temporal vector and raster data on the Apache Spark stack. For operators, we identify several possible algorithms and study how they can benefit from the underlying platform's properties. We further investigate how indexes can be realized in the distributed and parallel architecture of Big Data processing engines and compare methods for data partitioning, which perform differently well with respect to data skew and data set size. Furthermore, an approach to reduce the amount of data to process at operator level is presented. In order to reduce the execution times, we introduce an approach to transparently recycle intermediate results of dataflow programs, based on operator costs. To compute the costs, we instrument the programs with profiling code to gather the execution time and result size of the operators. In the evaluation, we first compare the various implementation and configuration possibilities in STARK and identify scenarios when and how partitioning and indexing should be applied. We further compare STARK to related systems and show that we can achieve significantly better execution times, not only when exploiting existing partitioning information. In the second part of the evaluation, we show that with the transparent cost-based materialization and recycling of intermediate results, the execution times of programs can be reduced significantly