Efecto antibacteriano del aceite esencial de Rosmarinus Officinalis (Romero) sobre Streptococcus Pyogenes ATCC 19615 comparado con amoxicilina in vitro

El estudio busca determinar la actividad antibacteriana in vitro del aceite esencial de Rosmarinus officinalis sobre Streptococcus pyogenes comparado con amoxicilina, para lo cual se utilizó aceite esencial de Rosmarinus officinalis, tiras con amoxicilina y cepas de Streptococus Pyogenes. Mediante destilación por arrastre con vapor se logró obtener el aceite esencial, para luego con dimetilsulfoxido diluir a 25%, 50%, 75% y 100%, posteriormente añadir aceite esencial de Rosmarinus officinalis y amoxicilina a tiras de papel absorbente, con el objetivo de colocar en los cultivos de Streptococus Pyogenes y determinar el efecto antibacteriano mediante el método de difusión de discos de kirby y Bauer. Se obtuvo como resultado que el Streptococus Pyogenes, presento sensibilidad a todas las concentraciones del aceite esencial, evidenciándose que al 100% presenta mayor efecto antibacteriano, siendo incluso mayor al de amoxicilina. En conclusión, se determinó que el aceite esencia de Rosmarinus Officinalis tiene mayor efecto antibacteriano que la amoxicilina in vitr

UJI AKTIVITAS MADU SEULAWAH SEBAGAI ANTIBAKTERI DALAM MENGHAMBAT PERTUMBUHAN STREPTOCOCCUS PYOGENES

ABSTRAKStreptococcus pyogenes merupakan salah satu bakteri yang sering dijumpai pada berbagai macam infeksi termasuk pada faringitis. Streptococcus pyogenes termasuk salah satu patogen yang sulit diobati jika terjadi resisten. Resistensinya terhadap beberapa antibiotik seringkali menyebabkan kegagalan pengobatan. Pengobatan dari bahan alam dinilai memiliki efek samping dan risiko lebih kecil dari bahan kimia serta mengurangi terjadinya resistensi obat. Salah satu bahan alami yang memiliki kandungan antibakteri adalah madu, Untuk mengetahui aktivitas antibakteri madu Seulawah terhadap pertumbuhan Streptococus pyogenes digunakan metode difusi cakram. Penelitian ini merupakan penelitian eksperimental dengan metode rancangan acak lengkap yang terdiri dari enam kelompok perlakuan dan empat kali pengulangan. Kelompok perlakuan menggunakan aquades sebagai kontrol negatif, amoxicillin sebagai kontrol positif, dan madu Seulawah dengan konsentrasi 25%, 50%, 75%, 100%. Data penelitian dianalisis dengan menggunakan uji One-Way ANOVA. Hasil penelitian ini menunjukkan madu Seulawah dengan konsentrasi 25%, 50%, 75%, 100% memiliki rata-rata daya hambat pada masing-masing konsentrasi sebesar 6,1 mm, 6,5 mm, 7,25 mm, 8,81 mm dan menurut kriteria morales secara deskriptif daya hambat madu seulawah terhadap pertumbuhan Streptococcus pyogenes tergolong hambatan yang lemah serta hasil uji One-Way ANOVA dan dilanjutkan uji Duncan menunjukkan adanya perbedaan yang bermakna pada masing-masing konsentrasi sehingga semakin tinggi konsentrasi madu Seulawah maka semakin besar daya hambat pertumbuhan terhadap bakteri Steprococcus pyogenes.Kata Kunci: Madu Seulawah, Streptococcus pyogenes, AntibakteriABSTRACTStreptococcus pyogenes is a bacterium that is often found in a variety of infections including in the pharyngitis. Streptococcus pyogenes is one of the pathogens that is difficult to treat if resistant occurs. Its resistance to several antibiotics often results in treatment failure. Treatment from natural ingredients is considered to have less effects of chemicals. The use of herbal also reduce the incidence of drug resistance. One of the natural ingredients that has antibacterial content is honey. To find out the antibacterial activity of Seulawah honey on the growth of Streptococus pyogenes, the disc diffusion method was used. This research is an experimental research with a completely randomized design method consisting of six treatment groups and four repetitions. The treatment group used aquades as a negative control, amoxicillin as a positive control, and Seulawah honey with concentrations of 25%, 50%, 75%, 100%. Research data were analyzed using the One-Way ANOVA test. The results of this study show that Seulawah honey with concentrations of 25%, 50%, 75%, 100% has an average inhibition strength at each concentration of 6,1 mm, 6,5 mm, 7,25 mm, 8,81 mm and according to the morales criteria descriptively the inhibitory ability of seulawah honey to the growth of Streptococcus pyogenes was classified relatively weak and the results of the One-Way ANOVA test and continued by Duncan test showed a significant difference in each concentration so that the higher the concentration of Seulawah honey the greater the inhibition growth against Steprococcus pyogenes bacteria.Keywords: Seulawah honey, Streptococcus pyogenes, Antibacteria

Antimicrobial Activities of Some Nigerian Chewing Sticks

The antimicrobial activities of the ethanolic extracts of three Nigerian chewing sticks, namely, Terminalia glaucescens, Anogeissus leiocarpus and Pseudocedrela kotschyi were investigated. Results from this study showed that the antimicrobial activities of the tested chewing sticks vary and are target-microbe specific. Of the tested chewing sticks, A. leiocarpus showed a significantly higher antibacterial activity (P\u3c0.05) against Staphylococcus aureus and Streptococcus pyogenes; and this was closely followed by T. glaucescens, while P. kotschyi virtually had no activity against these two organisms. However, the activity of T. glaucescens against Streptococcus mutans was significantly higher (P\u3c0.05) than that exhibited by A. leiocarpus. The extracts of the three chewing sticks had no activity against Candida albicans. The antibacterial activities of these two potent chewing sticks made them suitable for better dental care

Antimicrobial activities of Malaysian medicinal plants / Amirah Raihan Abdul Ra'of

The aim of this study is to determine the antimicrobial activity of the six plants extracts from Rhizomes of Curcuma longa (turmeric/kunyit), leaves of Phyllanthus acidus (Malay gooseberry/cermai), leaves of Murraya paniculata (orange jessamine/kemuning), leaves of Cassia alata (candle bush/gelenggang), leaves of Psidium gujava (guava/jambu batu), leaves of Centella asiatica (Indian pennywort/pegaga). Traditionally, these plants play important role in the domestic uses such as treating skin infections such itching, ringworm, and acne. Antimicrobial activities measured by serial dilution and disc diffusion assay. The antimicrobial activities of these plant extracts were tested using microorganism such Staphylococus aureus, Streptococus pyogenes, Esherichia coli, Pseudomonas aureginosa and Canadida albicans (yeast). From all these six plants, the most effective plants to kill microorganisms tested is Cassia alata. The antimicrobial activity of Cassia alata ethanol extract on Pseudomonas aeruginosa and Streptococus pyogenes were detecte

Efecto antibacteriano in vitro del aceite esencial de Origanum Vulgare sobre Streptococcus Pyogenes

Objetivo: Determinar el efecto antibacteriano in vitro del aceite esencial de origanum vulgare sobre Streptococcus pyogenes. Material y métodos: Se llevó a cabo un estudio de diseño experimental de observación múltiple. La población de estudio estuvo conformada por el conjunto de placas inoculadas con las cepas de Streptococus Pyogenes de muestras in vitro, aplicándoseles aceite de Origanum vulgare (orégano) observando su efecto antibacteriano para dichas sepas. Resultados: En el presente trabajo se encontró que el aceite de Origanum vulgare tuvo acción antibacteriana sobre Streptococcus pyogenes al compararlo con la eficacia de la amoxicilina, pero a pesar de ello ésta tuvo mayor efecto antibiótico mostando un halo de inhibición de 22.9mm a diferencia de del aceite que tuvo 12.9mm a una concentración de 100%. Comprobándose tener la amoxicilina una eficacia mayor sobre la cepa de S. pyogenes frente al aceite etanólico de Origanum vulgare (orégano). Conclusiones: Demostramos que existe efecto antibacteriano del aceite esencial de Origanum vulgare sobre Streptococcus pyogenes, pero a pesar de ello la amoxicilina supero los 19 mm en todos los casos. Demostramos que la concentración mínima inhibitoria del aceite esencial de Origanum vulgare sobre Streptococcus pyogenes es 14.031 μg/ml según 4 repeticiones

THE ANTIBACTERIAL ACTIVITY OF CLAUSENA ANISATA HOOK, A SOUTH AFRICAN MEDICINAL PLANT

Background: Clausena anisata Hook also known as Iperepesi in Xhosa language is a medicinal plant used traditionally for the treatment of various ailments and some opportunistic infections associated with tuberculosis (TB). Patients in South Africa based on the phytotherapeutic information on this species in the Eastern Cape, use this medicinal plant. Hence, the antibacterial activity of various solvent extracts of the leaves and barks were respectively, evaluated using selected bacterial strains. Method: The leaves and stem bark were tested against 10 selected strains of Gram - positive and Gram - negative bacteria through the agar dilution method. Acetone, dichloromethane and water extracts were used for the extraction. MIC was determined at different concentrations (0.1mg/ml, 0.5mg/ml, 1mg/ml and 5mg/ml) and the results obtained were compared to that of standard antibiotics. Result: The acetone extract of the leaves were more active against both Gram-positive and Gram –negative bacteria with MIC ranging from 0.1 mg/ml - 0.5 mg / ml. The dichloromethane extract of the bark showed appreciable activities against Staphylococcus aureus (ATCC 6538) (MIC: 0.1mg /ml) Escherichia coli and, Streptococcus pyogenes with an MIC of 5mg/ml respectively. On the other hand, the aqueous extract of the leaves showed no activity against the tested organisms with the exception of the aqueous bark extract which inhibited Staphylococcus aureus (MIC: 0.5mg/ml) and Pseudomonas aeruginosa (MIC: 5mg/ml). Conclusion: This study confirmed the antibacterial activities of acetone extract of the leaves of Clausena anisata. The capability of this extract to inhibit both Gram positive and negative bacteria is an indication that the extract is a potential broad spectrum antibacterial. The result of this study further justified its indigenous use for the treatment of bacteria commonly associated with TB especially among the people of Nkonkobe Municipality

Potential antibacterial effects of flaxseed and Nigella sativa extracts on 11 streptococcus pyogenes

Antibiotic resistance is a major global problem, associated with inadvertent drug usage. Herbal interventions are a therapeutic strategy that warrants greater research attention. Flaxseed and Nigella sativa are well recognized original super foods that have demonstrated potent anti-microbial and anti-biofilm activities. In the oral cavity, the bacterial population is a result of the dynamic relationship between pathogens and commensals Streptococcus pyogenes is an important global human Gram-positive pathogen that causes a wide variety of acute infections, it is highly virulent since it has the ability overcome the host defence system. This in vitro study aims to evaluate antimicrobial activity of flaxseed and Nigella sativa extract against S. pyogenes. Ethanolic extract of flaxseed and Nigella sativa extracts were prepared and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against S. pyogenes was estimated. The results of this study show that both extracts exhibited antibacterial activity against S. pyogenes. Present study demonstrated the bactericidal activity of both extracts which can be an adjunct to the future natural anti-bacterial therapy

Fascitis necrotitzant i miositis per streptococcus pyogenes

Existeixen tres tipus d'infeccions greus causades per Streptococcus del grup A (GAS): la fascitis necrotitzant (FN), la miositis i la síndrome de xoc tòxic estreptocòccica (SST). Es tracta d'un estudi retrospectiu en el qual es van revisar les històries clíniques de cinc casos de FN per Streptococcus pyogenes. Es descriuen dades epidemiològiques, característiques clíniques del pacient i de la infecció, paràmetres de laboratori, mètodes diagnòstics, tractaments rebuts i curs evolutiu dels pacients. Es va estimar la taxa de mortalitat de la cohort de pacients. El retard en el diagnòstic i tractament d'aquest tipus d'infeccions són les causes principals de mortalitat.Existen tres tipos de infecciones graves causadas por Streptococo del grupo A (GAS): la fascitis necrotizante (FN), la miositis y el síndrome de shock tóxico estreptocócico (SST). Se trata de un estudio retrospectivo en el que se revisaron las historias clínicas de cinco casos de FN por Streptococcus pyogenes. Se describen datos epidemiológicos, características clínicas del paciente y de la infección, parámetros de laboratorio, métodos diagnósticos, tratamientos recibidos y curso evolutivo de los pacientes. Se estimó la tasa de mortalidad de la cohorte de pacientes. El retraso en el diagnóstico y tratamiento de este tipo de infecciones son las causas principales de mortalidad

Anak Laki-laki Usia 3 Tahun Dengan Impetigo Krustosa Multipel

Latar Belakang. Salah satu bentuk pioderma adalah Impetigo, penyakit kulit yang menyerang lapisan epidermis kulit. Impetigo biasanya juga mengikuti trauma superficial dengan robekan kulit dan paling sering merupakan penyakit penyerta (secondary infection) dari pediculosis, skabies, infeksi jamur dan pada insect bites. Kasus. Pasien An. D, laki-laki usia 3 tahun datang dengan keluhan gatal dan kemerahan sejak 4 hari lalu. Kemerahan berubah menjadi gelembung yang berisi cairan, pecah dan menjadi krusta yang menebal. Status dermatologis tampak makula eritematosa miliar sampai lentikular, difus, anular, sirsinar, vesikel dan bula lentikular difus, pustule miliar sampai lentikular, krusta kuning kecoklatan, berlapis-lapis dan mudah diangkat. Simpulan. Telah ditegakkan diagnosis impetigo krustosa pada anak laki-laki usia 3 tahun berdasarkan anamnesis dan pemeriksaan fisik, serta telah di tatalaksana baik secara non-medikamentosa dan medikamentosa yang sesuai dengan evidence base medicine. [Medula Unila.2014;2(3):86-93

Antimicrobial Activity of Mushrooms against Skin Infection Causing Pathogens

Mushrooms are nutritionally functional food and a source of physiologically beneficial and nontoxic medicines for various diseased conditions. In the present study antimicrobial properties of crude extracts of three commercial edible mushrooms (Agaricus bisporus (J.E.Lange) Imbach , Pleurotus florida (Mont.) and Calocybe indica (P&A) were tested against bacteria and fungi that cause local dermatitis by disc diffusion method. Highest anti-microbial activity was obtained from petroleum ether extract of Agaricus bisporus, with the zone of inhibition 17mm (Streptococus pyogenes), 15mm (Staphylococus aureus), 14mm (Pseudomonas aeuroginosa) and 13mm (Candida albicans) from 100µg/ml concentration of mushroom extracts respectively, while minimal zone was obtained from the petroleum ether extract of Pleurotus florida and very least inhibition was observed in Calocybe indica. From the results it is inferred that crude extracts of commercially available mushrooms namely Agaricus bisporus and Pleurotus florida can be used to treat pathogenic microbes that cause skin irritations, bristles and acnes. This study gives scope for the investigations on active constituents of mushrooms for better understanding of the healing mechanism