Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance

Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic resistance genes (ARGs) is essential to develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing to analyse ARGs in 757 sewage samples from 243 cities in 101 countries, collected from 2016 to 2019. We find regional patterns in resistomes, and these differ between subsets corresponding to drug classes and are partly driven by taxonomic variation. The genetic environments of 49 common ARGs are highly diverse, with most common ARGs carried by multiple distinct genomic contexts globally and sometimes on plasmids. Analysis of flanking sequence revealed ARG-specific patterns of dispersal limitation and global transmission. Our data furthermore suggest certain geographies are more prone to transmission events and should receive additional attention.Peer reviewe

Global nitrogen and phosphate in urban wastewater for the period 1970 to 2050

This paper presents estimates for global N and P emissions from sewage for the period 1970-2050 for the four Millennium Ecosystem Assessment scenarios. Using country-specific projections for population and economic growth, urbanization, development of sewage systems, and wastewater treatment installations, a rapid increase in global sewage emissions is predicted, from 6.4 Tg of N and 1.3 Tg of P per year in 2000 to 12.0-15.5 Tg of N and 2.4-3.1 Tg of P per year in 2050. While North America (strong increase), Oceania (moderate increase), Europe (decrease), and North Asia (decrease) show contrasting developments, in the developing countries, sewage N and P discharge will likely increase by a factor of 2.5 to 3.5 between 2000 and 2050. This is a combined effect of increasing population, urbanization, and development of sewage systems. Even in optimistic scenarios for the development of wastewater treatment systems, global N and P flows are not likely to declin

Capability of the Invasive Tree Prosopis glandulosa Torr. to Remediate Soil Treated with Sewage Sludge

Sewage sludge improves agricultural soil and plant growth, but there are hazards associated with its use, including high metal(loid) contents. An experimental study was conducted under greenhouse conditions to examine the effects of sewage sludge on growth of the invasive tree Prosopis glandulosa, as well as to determine its phytoremediation capacity. Plants were established and grown for seven months along a gradient of sewage sludge content. Plant traits, soil properties, and plant and soil concentrations of N, P, K, Cd, Pb, Cu, Ni, Zn, Cr, Co, As, and Fe were recorded. The addition of sewage sludge led to a significant decrease in soil pH, and Ni, Co, and As concentrations, as well as an increase in soil organic matter and the concentrations of N, P, Cu, Zn, and Cr. Increasing sewage sludge content in the growth medium raised the total uptake of most metals by P. glandulosa plants due to higher biomass accumulation (taller plants with more leaves) and higher metal concentrations in the plant tissues. P. glandulosa concentrated more Cd, Pb, Cu, Zn, and Fe in its below-ground biomass (BGB) than in its above-ground biomass (AGB). P. glandulosa concentrated Ni, Co, and As in both BGB and AGB. P. glandulosa has potential as a biotool for the phytoremediation of sewage sludges and sewage-amended soils in arid and semi-arid environments, with a potential accumulation capability for As in plant leaves

Comparison of molecular markers to detect fresh sewage in environmental waters

Human-specific Bacteroides HF183 (HS-HF183), human-specific Enterococci faecium esp (HS-esp), human-specific adenoviruses (HS-AVs) and human-specific polyomaviruses (HS-PVs) assays were evaluated in freshwater, seawater and distilled water to detect fresh sewage. The sewage spiked water samples were also tested for the concentrations of traditional fecal indicators (i.e., Escherichia coli, enterococci and Clostridium perfringens) and enteric viruses such as enteroviruses (EVs), sapoviruses (SVs), and torquetenoviruses (TVs). The overall host-specificity of the HS-HF183 marker to differentiate between humans and other animals was 98%. However, the HS-esp, HS-AVs and HS-PVs showed 100% hostspecificity. All the human-specific markers showed >97% sensitivity to detect human fecal pollution. E. coli, enterococci and, C. perfringens were detected up to dilutions of sewage 10_5, 10_4 and 10_3 respectively.HS-esp, HS-AVs, HS-PVs, SVs and TVs were detected up to dilution of sewage 10_4 whilst EVs were detected up to dilution 10_5. The ability of the HS-HF183 marker to detect freshsewagewas3–4 orders ofmagnitude higher than that of the HS-esp and viral markers. The ability to detect fresh sewage in freshwater, seawater and distilled water matrices was similar for human-specific bacterial and viral marker. Based on our data, it appears that human-specific molecular markers are sensitive measures of fresh sewage pollution, and the HS-HF183 marker appears to be the most sensitive among these markers in terms of detecting fresh sewage. However, the presence of the HS-HF183 marker in environmental waters may not necessarily indicate the presence of enteric viruses due to their high abundance in sewage compared to enteric viruses. More research is required on the persistency of these markers in environmental water samples in relation to traditional fecal indicators and enteric pathogens

Will improving wastewater treatment impact shorebirds? Effects of sewage discharges on estuarine invertebrates and birds

Human communities often discharge wastewaters into estuaries, influencing their organic and pollutant loading, benthic community and trophic structure. The implementation of the Water Framework Directive has encouraged the treatment of wastewater discharges across European estuaries, but the implications for invertebrate and waterbird communities are poorly understood. We explore the effects of untreated sewage discharges on the distribution and abundance of foraging black-tailed godwits Limosa limosa and their main benthic prey (bivalves and polychaetes) on the Tejo estuary in Portugal, a major European Special Protection Area with ongoing wastewater improvements. Patches of mudflat in close proximity to sewage streams (70 m), and godwits foraging in these areas can attain the highest intake rates recorded for the estuary. However, high intake rates can also be attained on bivalve prey, and bivalve biomass and density increase slightly with distance from sewage streams. As the organic input from sewage outfalls influences invertebrate abundance and godwit foraging rates over relatively small areas, the ongoing implementation of a sewage treatment network on the Tejo estuary seems likely to have only a limited impact on the wintering godwit population. The localized effect of untreated sewage discharges on benthic communities suggests that the implications for predatory birds are relatively minor where alternative prey are available, but may be more severe in locations with more depauperate, polychaete-dominated invertebrate communities

Heavy metal content of vegetables irrigated with mixtures of wastewater and sewage sludge in Zimbabwe: Implications for human health

There is growing public concern in Zimbabwe over the illegal cultivation of vegetables on soils amended with sewage sludge or irrigated with admixtures of sewage and sewage sludge. Excessive accumulation of heavy metals in agricultural soils may not only result in environmental contamination, but lead to elevated heavy metal uptake by crops, which may affect food quality and safety. The work reported here studied heavy metal concentrations in crops irrigated with sewage sludge and sewage/sewage sludge admixtures at Firle Municipal Farm in Harare. The crops analysed in this study are heavily contaminated with the four regulated elements Cd, Cu, Pb and Zn. This contamination is at its highest in two of the staple dietary crops maize and tsunga. Tsunga leaves contained 3.68 mg kg-1 Cd, over 18 times the permissible level by the EU standards (0.2 mg kg-1); Cu concentrations were 111 mg kg-1, 5 times the EU Standard (20 mg kg-1); concentrations of Pb were 6.77 mg kg-1, over 22 times the permissible levels allowed by both EU standards and UK guidelines (0.3 mg kg-1); Zn concentrations were 221 mg kg-1, over 4 times the guideline value (50 mg kg-1). The other plants (beans, maize, peppers and sugarcane) also contained concentrations of heavy metals above the permissible levels. Furthermore the concentrations observed in this study were higher than those reported by other workers who have examined vegetation from other contaminated sites. This study highlights the potential risks involved in the cultivation and consumption of vegetables on plots irrigated with sewage sludge, a practice which may place at risk the health of the urban population who consume these vegetables

Sewage analyses for antibiotic resistance within fecal E. coli isolates : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University

This investigation was undertaken to explore possible surveillance methods which might be applied in surveys of the incidence of acquired antibiotic resistance in fecal bacteria being shed by an urban population; the Palmerston North City sewage system served as a sampling device. Fecal E. coli was used as an indicator organism by virtue of its inherent sensitivity to several relevant antibiotics and, further, by virtue of the fact that antibiotic resistance in this microorganism can, in general, be attributed to plasmids coding for the resistance character(s) In the course of these exploratory studies it was observed that fecal E. coli accounted for 6 to 14% of the total coliforms present in sewage samples; the number of fecal E. coli in any given sewage sample was affected by the flow rate of the sewage and the rainfall

A note on sewage sludge - risk assessments and fertilization value

A number of recent studies of risk related to agricultural use of sewage sludge are reviewed, as a contribution to the discussion of potential for use in organic agriculture. Furthermore a very tentative assessment of the fertilization value of sewage sludge and its derived products is developed

Treatment and removal strategies for estrogens from wastewater

Natural and synthetic steroidal estrogens (estrone, 17β-estradiol and 17α-ethinylestradiol) are endocrine disrupters, that are discharged consistently from the sewage treatment works into surface waters, thereby causing endocrine disrupting effects to aquatic organisms at trace concentrations (nanogram per litre). Several years of research have been focused on their fate, behaviour and removal in the environment but primarily in the sewage treatment works which acts as a sink for these compounds. This review attempts to summarize the factors involved in the removal of these chemicals from the sewage treatment works. Biological processes, and to a limited extent physio-chemical properties, play a vital role in the endocrinal deactivation of which these compounds. The efficiency of these processes is highly dependent on operating parameters (such as sludge retention time, redox potential, etc) that govern the secondary treatment process of a functional sewage treatment works. Although advanced treatment technologies are available, cost and operational considerations do not make them a sustainable solution

Sustainable recycling of compost products in plant production

The project aims at improving the quality of composts made of municipal biowaste and sewage sludge and at developing the quality control of composts. The most important objective is to study and verify the effects of the use of biowaste and sewage sludge composts in plant production under Finnish conditions