Evaluation of tomato varieties for their use by small organic farmers in Buenos Aires, Argentina

A significant increase in the domestic organic market, especially in Buenos Aires city can be observed in Argentina. Horticultural products represent a large percentage of the total organic volume traded. Within these, increasingly evident interest in heritage type products, niche type or heirloom can be observed. As the development of these products is almost incipient, varieties of tomatoes were rescued to characterize its production profile for possible adoption by organic horticultural producers in the area of Buenos Aires. A greenhouse trial with 14 varieties of tomatoes was developed. They were: PeaceVein, Ildi Red and Yellow, Black Plum, Chadwick, Saint Pierre, Thessaloniki, TSW10, Platense Gentile, Money maker, Mars, Uco Plata and the hybrid Koyi (from TheRuiterSeeds) and Elpida (Syngenta). Phenological and reproductive characteristics and agronomic profile of each variety were evaluated. The results concluded that some varieties are suitable to be incorporated to the horticultural trade as a complement of organic vegetables in the Horticultural Greenbelt in Buenos Aires. The most important varieties were the Ildis ones, with attractive color and piriformis; Black Plum, an old mahogany ovate tomato variety; Chadwick, also known as very productive variety of tasty fruit. Those who need further research include tomato Platense Gentile and Uco Plata

Intervención psicológica con corredores de maratón : características y valoración del programa aplicado en el maratón de Madrid

12 psicólogos del deporte participaron en un programa de intervención psicológica con los corredores del Maratón de Madrid. En los días anteriores al maratón, 1457 corredores recibieron asesoramiento a través de un folleto informativo con recomendaciones prácticas para preparar su participación en la carrera (incluyendo establecimiento de objetivos, planificación de la carrera, anticipación de dificultades, control de pensamientos negativos y control de la activación), información relacionada con sus puntuaciones en el cuestionario CSAI-2 (ansiedad somática, ansiedad cognitiva y autoconfianza) y atención de demandas concretas. Después de la carrera, los psicólogos proporcionaron apoyo psicológico a corredores que acudieron al servicio de Fisioterapia. Así mismo, antes de la prueba, el personal sanitario (fisioterapeutas y enfermeros) recibió algunas recomendaciones prácticas para optimizar su trabajo con los corredores. Unas semanas más tarde, 452 corredores contestaron a una encuesta valorando la eficacia de esta intervención psicológica, recogiéndose, también, los informes globales de los psicólogos participantes y de un reducido número de fisioterapeutas. Los datos senalan que el asesoramiento realizado antes de la carrera fue considerado útil por la mayoría de los corredores y bastante útil para un considerable número de ellos, sobre todo para aquellos que lograron el objetivo deportivo que habían establecido para este maratón, destacando la información recibida para el control de pensamientos negativos y en relación con la autoconfianza. Las valoraciones globales de corredores, psicólogos y fisioterapeutas también avalan la utilidad del apoyo psicológico proporcionado después de la carrera. En el artículo se explican las características de este programa de intervención psicológica con corredores populares de maratón, y se discute su aportación a la Psicología del Deporte, al papel de los psicólogos del deporte en equipos interdisciplinares y a la contribución de los psicólogos en los maratones populares.12 sport psychologists conducted a programme of psychological intervention with the participants at the Marathon of Madrid (Spain). In the days prior the marathon 1457 recreational runners were helped with psychological counselling using both a handout with practical recommendations to prepare for the race (including goal setting, planning for the race, anticipating main difficulties, preparing to control negative thoughts, and arousal control), information related to their scores in the CSAI-2 (somatic anxiety, cognitive anxiety and self-confidence), and attention to specific demands. After the race, psychologists provided psychological support to runners who attended the service of Physiotherapy to receive massage. Likewise, before the race, the medical personnel involved in te marathon (physiotherapists and nurses) received from sport psychologists practical guidelines to improve their work with the runners. Few weeks after the marathon, 452 runners answered to a survey to assess this programme. Data were also obtained through global reports from the sport psychologists and a reduced number of physiotherapists. Most runners found useful, and many of them very useful, the counselling provided before the race, especially runners who achieved their goals for the marathon, highlighting the information to control negative thoughts and related to selfconfidence. Global reports of runners, sport psychologists and physiotherapists, also supported the utility of psychological intervention after the race, especially to help runners to relax, to tolerate pain while receiving massage, to show their feelings and to gain control over the experience. The article explains all the characteristics of this programme and pinpoints its contribution to Sport Psychology, the role of sport psychologists within multidisciplinary teams, and the contribution of sport psychologists in recreational marathons

Effect of GnRH or Estradiol Benzoate on Reproductive Traits during a Heatsynch Protocol in Dairy Cows

Background: Milk production of cows is closely correlated with its reproductive efficiency. One of the several factors influencing the dairy efficiency is the decline in fertility of the cows/heifers. Use of hormonal protocol are often used to improve the fertility of the recipients. Several programs are available to inseminate dairy cows, with variation in the use of different types of hormones, time of use and range of duration period, as well as the time of AI. The objective of this study was to evaluate the efficiency of follicular wave emergence induction of estradiol benzoate in comparison to GnRH in a Heatsynch protocol and its influence on reproductive parameters.Materials, Methods & Results: In this study were used primiparous and multiparous Holstein cows (n = 325). Animals were divided randomly into two different treatments according with the hormone used to induce follicular wave emergence (D0). The GnRH group (n = 167), was given 25 mg, while in the EB group (n = 158) was used 2 mg. At same day, were inserted, a controlled internal drug-releasing containing 1.9 g of progesterone CIDR®. On day 7, CIDR was removed and cows were given 25 mg luteinizing hormone followed by an injection of 1 mg Inducer Ovulation at day 8. Cows were then observed for signs of estrus for at least two hours (twice daily at 7 am and 6 pm) for three days following CIDR® removal. Cows detected in estrus were inseminated 12 h later the estrus detection. Pregnancy status was determined by ultrasonography at 30 ± 5 d and 60 ± 5 d after AI. A single technician was responsible for the estrus detection and inseminations. A single technician was responsible for the estrus detection and inseminations. Ultrassonographic examinations with ultrasound, transrectal linear probe of 5 MHz, were performed for the diagnosis of pregnancy at 30 and 60 days after artificial insemination. Binomially distributed data, such as, estrous response, conception rate, pregnancy rate and pregnancy losses, were analyzed by Chi-square test. Pregnancy rates at 30-35 days and 60-65 days as well as pregnancy losses were considered in the model for the effect of parity (primiparous vs. multiparous) and the group of estrus behavior during the Heatsynch protocol. Conception rate was similar between GnRH (36.1%, 57/158) and EB (37.7%, 63/167) treatments. Cows showing estrus signs after CIDR removed were 71.9% in GnRH and 62% in EB group (P = 0.05). Embryonic losses were different (P = 0.03) according to groups of cows showing estrus signals or not.Discussion: Our results demonstrate that conception rates are not affected by using either EB or GnRH at the beginning of the Heatsynch protocol. Therefore, the EB can be an alternative choice to GnRH at Heatsynch beginning. Similar results were found by autor in dairy cows, once it was also observed that there is no difference in conception rate in two TAI protocols using either GnRH or EB. In Heatsynch protocols the increase in estrus detection are a key factor to increase fertility in lactating dairy cows. It can be explained once animals in regular estrous cycle have a palpable corpus luteum and circulating progesterone concentration higher than 1 ng/mL which leads than to enhance pregnancy ratios. The use of BE at the time of CIDR insertion in TAI protocols for dairy cattle proved to be an alternative to GnRH considering conception rates. Therefore, the use of BE at the time of CIDR insertion in a Heatsynch protocol in dairy cattle proved to be an alternative to GnRH which can be an important tool to increase the TAI utilization by farmers. Furthermore, in Heatsynch protocol cows showing estrus have pregnancy rates improved and lower pregnancy losses

VLTI status update: a decade of operations and beyond

We present the latest update of the European Southern Observatory's Very Large Telescope interferometer (VLTI). The operations of VLTI have greatly improved in the past years: reduction of the execution time; better offering of telescopes configurations; improvements on AMBER limiting magnitudes; study of polarization effects and control for single mode fibres; fringe tracking real time data, etc. We present some of these improvements and also quantify the operational improvements using a performance metric. We take the opportunity of the first decade of operations to reflect on the VLTI community which is analyzed quantitatively and qualitatively. Finally, we present briefly the preparatory work for the arrival of the second generation instruments GRAVITY and MATISSE.Comment: 10 pages, 7 figures, Proceedings of the SPIE, 9146-1

Proper Motions and Orbits of Distant Local Group Dwarf Galaxies from a combination of Gaia and Hubble Data

We have determined the proper motions (PMs) of 12 dwarf galaxies in the Local Group (LG), ranging from the outer Milky Way (MW) halo to the edge of the LG. We used HST as the first and Gaia as the second epoch using the GaiaHub software. For Leo A and Sag DIG we also used multi-epoch HST measurements relative to background galaxies. Orbital histories derived using these PMs show that two-thirds of the galaxies in our sample are on first infall with $>$90\% certainty. The observed star formation histories (SFHs) of these first-infall dwarfs are generally consistent with infalling dwarfs in simulations. The remaining four galaxies have crossed the virial radius of either the MW or M31. When we compare their star formation (SF) and orbital histories we find tentative agreement between the inferred pattern of SF with the timing of dynamical events in the orbital histories. For Leo~I, SF activity rises as the dwarf crosses the MW's virial radius, culminating in a burst of SF shortly before pericenter ($\approx1.7$~Gyr ago). The SF then declines after pericenter, but with some smaller bursts before its recent quenching ($\approx0.3$~Gyr ago). This shows that even small dwarfs like Leo~I can hold on to gas reservoirs and avoid quenching for several Gyrs after falling into their host, which is longer than generally found in simulations. Leo~II, NGC~6822, and IC~10 are also qualitatively consistent with this SF pattern in relation to their orbit, but more tentatively due to larger uncertainties.Comment: 27 pages, 9 Figures, 8 Tables, Submitted to Ap

Delineating Human B Cell Precursor Development With Genetically Identified PID Cases as a Model

B-cell precursors (BCP) arise from hematopoietic stem cells in bone marrow (BM). Identification and characterization of the different BCP subsets has contributed to the understanding of normal B-cell development. BCP first rearrange their immunoglobulin (Ig) heavy chain (IGH) genes to form the pre-B-cell receptor (pre-BCR) complex together with surrogate light chains. Appropriate signaling via this pre-BCR complex is followed by rearrangement of the Ig light chain genes, resulting in the formation, and selection of functional BCR molecules. Consecutive production, expression, and functional selection of the pre-BCR and BCR complexes guide the BCP differentiation process that coincides with corresponding immunophenotypic changes. We studied BCP differentiation in human BM samples from healthy controls and patients with a known genetic defect in V(D)J recombination or pre-BCR signaling to unravel normal immunophenotypic changes and to determine the effect of differentiation blocks caused by the specific genetic defects. Accordingly, we designed a 10-color antibody panel to study human BCP development in BM by flow cytometry, which allows identification of classical preB-I, preB-II, and mature B-cells as defined via BCR-related markers with further characterization by additional markers. We observed heterogeneous phenotypes associated with more than one B-cell maturation pathway, particularly for the preB-I and preB-II stages in which V(D)J recombination takes place, with asynchronous marker expression patterns. Next Generation Sequencing of complete IGH gene rearrangements in sorted BCP subsets unraveled their rearrangement status, indicating that BCP differentiation does not follow a single linear pathway. In conclusion, B-cell development in human BM is not a linear process, but a rather complex network of parallel pathways dictated by V(D)J-recombination-driven checkpoints and pre-BCR/BCR mediated-signaling occurring during B-cell production and selection. It can also be described as asynchronous, because precursor B-cells do not differentiate as full population between the different stages, but rather transit as a continuum, which seems influenced (in part) by V-D-J recombination-driven checkpoints

Dissection of the pre-germinal center B-cell maturation pathway in common variable immunodeficiency based on standardized flow cytometric EuroFlow tools

Copyright © 2021 del Pino-Molina, López-Granados, Lecrevisse, Torres Canizales, Pérez-Andrés, Blanco, Wentink, Bonroy, Nechvatalova, Milota, Kienzler, Philippé, Sousa, van der Burg, Kalina, van Dongen and Orfao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Introduction: Common Variable Immunodeficiency (CVID) is characterized by defective antibody production and hypogammaglobulinemia. Flow cytometry immunophenotyping of blood lymphocytes has become of great relevance for the diagnosis and classification of CVID, due to an impaired differentiation of mature post-germinal-center (GC) class-switched memory B-cells (MBC) and severely decreased plasmablast/plasma cell (Pb) counts. Here, we investigated in detail the pre-GC B-cell maturation compartment in blood of CVID patients. Methods: In this collaborative multicentric study the EuroFlow PID 8-color Pre-GC B-cell tube, standardized sample preparation procedures (SOPs) and innovative data analysis tools, were used to characterize the maturation profile of pre-GC B-cells in 100 CVID patients, vs 62 age-matched healthy donors (HD). Results: The Pre-GC B-cell tube allowed identification within pre-GC B-cells of three subsets of maturation associated immature B-cells and three subpopulations of mature naïve B-lymphocytes. CVID patients showed overall reduced median absolute counts (vs HD) of the two more advanced stages of maturation of both CD5+ CD38+/++ CD21het CD24++ (2.7 vs 5.6 cells/µl, p=0.0004) and CD5+ CD38het CD21+ CD24+ (6.5 vs 17 cells/µl, p1 (CD38, CD5, CD19, CD21, CD24, and/or smIgM) phenotypic marker (57/88 patients; 65%) for a total of 3 distinct CVID patient profiles (group 1: 42/88 patients, 48%; group 2: 8/88, 9%; and group 3: 7/88, 8%) and ii) CVID patients with a clearly altered pre-GC B cell maturation pathway in blood (group 4: 31/88 cases, 35%). Conclusion: Our results show that maturation of pre-GC B-cells in blood of CVID is systematically altered with up to four distinctly altered maturation profiles. Further studies, are necessary to better understand the impact of such alterations on the post-GC defects and the clinical heterogeneity of CVID.The coordination and innovation processes of this study were supported by the EuroFlow Consortium (Chairmen: MB and AO). LP-M was supported by FIS PI16/01605 and JTC by FIS PI13/02296 (Fondo de Investigación Sanitaria Instituto de Salud Carlos III, Madrid, Spain). The work was partially supported by grant PI20/01712-FEDER (Fondo de Investigación Sanitaria Instituto de Salud Carlos III, Madrid, Spain) and a grant from Fundación Mutua Madrileña (MMA, Madrid, Spain).info:eu-repo/semantics/publishedVersio