The Method of Establishing Analogy Reservoir for Low Permeability Sandstone Reservoir

Analogy reservoir is accurate or not, related to the reliability of the SEC reserves evaluation. According to the characteristics of Jilin Oilfield low permeability sandstone reservoir, in accordance with SEC new rules and the principle of analogy reservoir establishment, we establish analogy reservoir of low permeability sandstone reservoir by taking the steps of selecting analogy reservoir, calculating geological reserves, evaluating recoverable reserves, and defining analogy recovery. With this method, we set up analogy reservoir sequence of low permeability sandstone reservoir and the sequence provides a reliable basis for SEC reserves evaluation. Key words: SEC rules; Geological reserves; Recoverable reserves; Analogy reservoir; Recovery efficienc

Muti-frequency extended sampling method for the inverse acoustic source problem

We consider the reconstruction of the compact support of an acoustic source given multiple frequency far field data. We propose a multi-frequency extended sampling method (MESM). The MESM computes the solutions of some ill-posed integral equations and constructs an indicator function to image the source. The behavior of the indicator function is justified. The method is fast and easy to implement. Various numerical examples are presented to show the effectiveness of the MESM for both frequency-independent and frequency-dependent sources

Forage Genomics Accelerate the Germplasm Resource Innovation

To achieve sustainability and food security we need expand the germplasm base and access novel genetic diversity to accelerate breeding. For developing new forage cultivars, the availability of a high-quality genome facilitates accurate characterization of new germplasm, and an understanding of the genetics underlying important traits. Here, we sequenced and assembled three high-quality chromosome-level forage genomes. The contig-level assembly of Cleistogenes songorica (2n = 4x = 40) comprised 540.12 Mb of the genome, with a contig N50 of 21.28 Mb. Complete assemblies of all telomeres, and of ten chromosomes were derived. The chromosome-scale genome size of elephant grass (2n = 4x = 28) was 1.97 Gb and heterozygosity rate was 1.5%. The chromosome-scale genome size of Melilotus albus (2n = 2x = 16) was 1.04 Gb, containing 71.42% repetitive elements. This study provides implementation pathways to study genome evolution, adaptation to stress and genetic basis of unique or complex traits in three species. The genomic resources that we developed in this study offer valuable information that will facilitate efficient germplasm exploration and genetic improvement of the three species for pasture uses

Nowhere to Go: Benchmarking Multi-robot Collaboration in Target Trapping Environment

Collaboration is one of the most important factors in multi-robot systems. Considering certain real-world applications and to further promote its development, we propose a new benchmark to evaluate multi-robot collaboration in Target Trapping Environment (T2E). In T2E, two kinds of robots (called captor robot and target robot) share the same space. The captors aim to catch the target collaboratively, while the target will try to escape from the trap. Both the trapping and escaping process can use the environment layout to help achieve the corresponding objective, which requires high collaboration between robots and the utilization of the environment. For the benchmark, we present and evaluate multiple learning-based baselines in T2E, and provide insights into regimes of multi-robot collaboration. We also make our benchmark publicly available and encourage researchers from related robotics disciplines to propose, evaluate, and compare their solutions in this benchmark. Our project is released at https://github.com/Dr-Xiaogaren/T2E

Incidence and fatality of serious suicide attempts in a predominantly rural population in Shandong, China: a public health surveillance study

Objectives To estimate the incidence of serious suicide attempts (SSAs, defined as suicide attempts resulting in either death or hospitalisation) and to examine factors associated with fatality among these attempters. Design A surveillance study of incidence and mortality. Linked data from two public health surveillance systems were analysed. Setting Three selected counties in Shandong, China. Participants All residents in the three selected counties. Outcome measures Incidence rate ( per 100 000 person-years) and case fatality rate (%). Methods Records of suicide deaths and hospitalisations that occurred among residents in selected counties during 2009–2011 (5 623 323 person-years) were extracted from electronic databases of the Disease Surveillance Points (DSP) system and the Injury Surveillance System (ISS) and were linked by name, sex, residence and time of suicide attempt. A multiple logistic regression model was developed to examine the factors associated with a higher or lower fatality rate. Results The incidence of SSAs was estimated to be 46 (95% CI 44 to 48) per 100 000 person-years, which was 1.5 times higher in rural versus urban areas, slightly higher among females, and increased with age. Among all SSAs, 51% were hospitalised and survived, 9% were hospitalised but later died and 40% died with no hospitalisation. Most suicide deaths (81%) were not hospitalised and most hospitalised SSAs (85%) survived. The fatality rate was 49% overall, but was significantly higher among attempters living in rural areas, who were male, older, with lower education or with a farming occupation. With regard to the method of suicide, fatality was lowest for non-pesticide poisons (7%) and highest for hanging (97%). Conclusions The incidence of serious suicide attempts is substantially higher in rural areas than in urban areas of China. The risk of death is influenced by the attempter’s sex, age, education level, occupation, method used and season of year

Bridging multiscale interfaces for developing ionically conductive high-voltage iron sulfate-containing sodium-based battery positive electrodes

Non-aqueous sodium-ion batteries (SiBs) are a viable electrochemical energy storage system for grid storage. However, the practical development of SiBs is hindered mainly by the sluggish kinetics and interfacial instability of positive-electrode active materials, such as polyanion-type iron-based sulfates, at high voltage. Here, to circumvent these issues, we proposed the multiscale interface engineering of Na$_{2.26}$Fe$_{1.87}$(SO$_4$)$_3$, where bulk heterostructure and exposed crystal plane were tuned to improve the Na-ion storage performance. Physicochemical characterizations and theoretical calculations suggested that the heterostructure of Na$_6$Fe(SO$_4$)$_4$ phase facilitated ionic kinetics by densifying Na-ion migration channels and lowering energy barriers. The (11-2) plane of Na$_{2.26}$Fe$_{1.87}$(SO$_4$)$_3$ promoted the adsorption of the electrolyte solution ClO4− anions and fluoroethylene carbonate molecules, which formed an inorganic-rich Na-ion conductive interphase at the positive electrode. When tested in combination with a presodiated FeS/carbon-based negative electrode in laboratory- scale single-layer pouch cell configuration, the Na$_{2.26}$Fe$_{1.87}$(SO$_4$)$_3$-based positive electrode enables an initial discharge capacity of about 83.9 mAh g$^{−1}$, an average cell discharge voltage of 2.35 V and a specific capacity retention of around 97% after 40 cycles at 24 mA g$^{−1}$ and 25 °C

A CRISPR/Cas12a-assisted rapid detection platform by biosensing the apxIVA of Actinobacillus pleuropneumoniae

Actinobacillus pleuropneumoniae is an important respiratory pig pathogen that causes substantial losses in the worldwide swine industry. Chronic or subclinical infection with no apparent clinical symptoms poses a challenge for preventing transmission between herds. Rapid diagnostics is important for the control of epidemic diseases. In this study, we formulated an A. pleuropneumoniae species-specific apxIVA-based CRISPR/Cas12a-assisted rapid detection platform (Card) that combines recombinase polymerase amplification (RPA) of target DNA and subsequent Cas12a ssDNase activation. Card has a detection limit of 10 CFUs of A. pleuropneumoniae, and there is no cross-reactivity with other common swine pathogens. The detection process can be completed in 1 h, and there was 100% agreement between the conventional apxIVA-based PCR and Card in detecting A. pleuropneumoniae in lung samples. Microplate fluorescence readout enables high-throughput use in diagnostic laboratories, and naked eye and lateral flow test readouts enable use at the point of care. We conclude that Card is a versatile, rapid, accurate molecular diagnostic platform suitable for use in both laboratory and low-resource settings

Cloning and Functional Analysis of the MADS-box CiMADS9 Gene from Carya illinoinensis

AbstractA MADS-box gene, CiMADS9, was cloned from the male flowers of Carya illinoinensis by rapid amplification of cDNA ends. The gene was 1 077bp with a 768bp open reading frame encoding 255 amino acids. Multiple sequence comparisons revealed that CiMADS9 is a typical MIKC-type MADS-box gene with a MADS-box domain and a K semi-conserved region. Phylogenetic analysis indicated that CiMADS9 belongs to the AGL15 group of the MADS-box gene family. Quantitative reverse transcription polymerase chain reaction analysis indicated that the expression levels in reproductive organs (i.e., flowers and young fruits) were considerably higher than in vegetative tissues (i.e., leaves and branches). The highest expression levels were observed in male flowers. An overexpression vector for CiMADS9 was constructed and the gene was inserted into the Arabidopsis thaliana genome. CiMADS9 expression was confirmed in all transgenic lines. Compared with wild-type plants, transgenic A. thaliana plants overexpressing CiMADS9 exhibited delayed flowering and an increased number of leaves

Understanding the Passivation Mechanisms and Opto-Electronic Spectral Response in Methylammonium Lead Halide Perovskite Single Crystals

Attaining control over the surface traps in halide perovskites is critical for the tunability of ultimate device characteristics. Here, we present a study on the modulation of photophysical properties, surface traps, and recombination in MAPbI(3) single crystals (MSCs) with methylamine (MA) vapor surface treatment. Transient photoluminescence spectroscopy in conjunction with density functional theory calculations reveals that nonradiative recombination related to Pb2+ becomes mitigated after MA vaporing while radiative recombination via bimolecular path tends to increase, which originates from the passivation of Pb ions with the Lewis base nitrogen in MA. In contrast to the broad photoresponse in the pristine MSC photodiodes, application of MA surface treatments leads to a spectral narrowing effect (SNE) in MSCs with the response peak width</p