Variability in the interpretation of probability phrases used in Dutch news articles — a risk for miscommunication

Analysis and Stochastic

An overview of activity-based probes for glycosidases

As the scope of modern genomics technologies increases, so does the need for informative chemical tools to study functional biology. Activity-based probes (ABPs) provide a powerful suite of reagents to probe the biochemistry of living organisms. These probes, featuring a specificity motif, a reactive chemical group and a reporter tag, are opening-up large swathes of protein chemistry to investigation in vitro, as well as in cellular extracts, cells and living organisms in vivo. Glycoside hydrolases, by virtue of their prominent biological and applied roles, provide a broad canvas on which ABPs may illustrate their functions. Here we provide an overview of glycosidase ABP mechanisms, and review recent ABP work in the glycoside hydrolase field, encompassing their use in medical diagnosis, their application for generating chemical genetic disease models, their fine-tuning through conformational and reactivity insight, their use for high-throughput inhibitor discovery, and their deployment for enzyme discovery and dynamic characterization.Medical BiochemistryBio-organic Synthesi

De vorming en stabiliteit van L.C.P. vezels in een thermoplastische matrix

Bij dit onderzoek is geprobeerd meer duidelijkheid te krijgen omtrent de stabiliteit van L.C.P. vezels die kunnen ontstaan tijdens verwerking van L. C. P. /thermoplast mengsels. Daarnaast is onderzocht hoe de morfologie van deze mengsels tijdens extrusie ontstaat. Hierbij is vooral de rol van afschuiving bekeken. Er is gewerkt met Vectra A900 -een thermotroop L.C.P.- en SEBS Kraton G-1650 -een thermoplastische rubber-.Applied SciencesTechnologie van Macromolekulaire Stoffe

A multiplexing activity-based protein profiling platform for dissection of a native bacterial xyloglucan-degrading system

Bacteria and yeasts grow on biomass polysaccharides by expressing and excreting a complex array of glycoside hydrolase (GH) enzymes. Identification and annotation of such GH pools, which are valuable commodities for sustainable energy and chemistries, by conventional means (genomics, proteomics) is complicated, as primary sequence or secondary structure alignment with known active enzymes is not always predictive for new ones. Here we report a “low-tech”, easy-to-use and sensitive, multiplexing activity-based protein profiling platform to characterize the xyloglucan-degrading GH system excreted by the soil saprophyte, Cellvibrio japonicus when grown on xyloglucan. A suite of activity-based probes bearing orthogonal fluorophores allows for the visualization of accessory exo-acting glycosidases, which are then identified using biotin-bearing probes. Substrate specificity of xyloglucanases is directly revealed by imbuing xyloglucan structural elements into bespoke activity-based probes. Our ABPP platform provides a highly useful tool to dissect xyloglucan-degrading systems from various sources and to rapidly select potentially useful ones. The observed specificity of the probes moreover bodes well for the study of other biomass polysaccharide degrading systems, by modelling probe structures to those of desired substrates

Energy restriction and Roux-en-Y gastric bypass reduce postprandial alpha-dicarbonyl stress in obese women with type 2 diabetes

Conclusions/interpretation This study shows that type 2 diabetes is characterised by increased fasting and postprandial plasma alpha-dicarbonyl stress, which can be reduced by improving glucose metabolism through a VLCD or RYGB. These data highlight the potential to reduce reactive alpha-dicarbonyls in obese individuals with type 2 diabetes. Trial registration: ClinicalTrials.gov NCT01167959Functional Genomics of Systemic Disorder

Playing Tetris to counteract the development of intrusive memories of trauma - A large sample multisite trial

Independent multi-site replication of Holmes et al. (2009) on the effects of playing Tetris on the development of intrusive memories of a traumafilm