Patron Driven Acquisition: Wie wird die Ebook Library (EBL) an der Universität Leipzig genutzt?

In this study, an in-depth analysis of usage data covering one year of patron-driven acquisition on the ebook platform Ebook Library (EBL) is presented. A special focus lies on the evaluation of use and re-use of short term loans. As a result, relevant licensing parameters can be adjusted according to existing usage patterns and a more efficient operation of PDA can be achieve

Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga \u3ci\u3eNannochloropsis oceanica\u3c/i\u3e CCMP1779

Unicellular marine algae have promise for providing sustainable and scalable biofuel feedstocks, although no single species has emerged as a preferred organism. Moreover, adequate molecular and genetic resources prerequisite for the rational engineering of marine algal feedstocks are lacking for most candidate species. Heterokonts of the genus Nannochloropsis naturally have high cellular oil content and are already in use for industrial production of high-value lipid products. First success in applying reverse genetics by targeted gene replacement makes Nannochloropsis oceanica an attractive model to investigate the cell and molecular biology and biochemistry of this fascinating organism group. Here we present the assembly of the 28.7 Mb genome of N. oceanica CCMP1779. RNA sequencing data from nitrogen-replete and nitrogendepleted growth conditions support a total of 11,973 genes, of which in addition to automatic annotation some were manually inspected to predict the biochemical repertoire for this organism. Among others, more than 100 genes putatively related to lipid metabolism, 114 predicted transcription factors, and 109 transcriptional regulators were annotated. Comparison of the N. oceanica CCMP1779 gene repertoire with the recently published N. gaditana genome identified 2,649 genes likely specific to N. oceanica CCMP1779. Many of these N. oceanica–specific genes have putative orthologs in other species or are supported by transcriptional evidence. However, because similarity-based annotations are limited, functions of most of these species-specific genes remain unknown. Aside from the genome sequence and its analysis, protocols for the transformation of N. oceanica CCMP1779 are provided. The availability of genomic and transcriptomic data for Nannochloropsis oceanica CCMP1779, along with efficient transformation protocols, provides a blueprint for future detailed gene functional analysis and genetic engineering of Nannochloropsis species by a growing academic community focused on this genus

Patron Driven Acquisition: Wie wird die Ebook Library (EBL) an der Universität Leipzig genutzt?

In this study, an in-depth analysis of usage data covering one year of patron-driven acquisition on the ebook platform Ebook Library (EBL) is presented. A special focus lies on the evaluation of use and re-use of short term loans. As a result, relevant licensing parameters can be adjusted according to existing usage patterns and a more efficient operation of PDA can be achieve

Patron Driven Acquisition: Wie wird die Ebook Library (EBL) an der Universität Leipzig genutzt?

In this study, an in-depth analysis of usage data covering one year of patron-driven acquisition on the ebook platform Ebook Library (EBL) is presented. A special focus lies on the evaluation of use and re-use of short term loans. As a result, relevant licensing parameters can be adjusted according to existing usage patterns and a more efficient operation of PDA can be achieve

Legislative Documents

Also, variously referred to as: House bills; House documents; House legislative documents; legislative documents; General Court documents

„Sind Sie ein Mensch?“: Chat und Weblog an der UB Leipzig

Wenn sich Bibliotheksmitarbeiterin und Bibliotheksnutzer nicht von Angesicht zu Angesicht gegenüber stehen, ist diese Frage berechtigt: Sind Sie ein Mensch? Oder sind Sie ein automatisierter Chatroboter – vergleichbar mit telefonischen Informationshotlines, die wohl jeder aus leidvoller Erfahrung kennt? Gestellt wurde uns diese Frage tatsächlich – und mehrfach – von Bibliotheksnutzerinnen und -nutzern, die ihr Glück kaum fassen konnten, wie einfach es sein kann, eine Antwort direkt online zu bekommen. Es muss nicht immer die Auskunftstheke sein. Virtuell hilft auch

A Lipid Droplet Protein of Nannochloropsis with Functions Partially Analogous to Plant Oleosins1[W][OA]

As our understanding of the dynamics of lipid droplets (LDs) in animal, plant, and fungal cells is rapidly evolving, still little is known about the formation and turnover of these organelles in microalgae. Yet with the growing importance of algal feedstock for the production of biofuels and high-value lipids, there is a need to understand the mechanisms of LD dynamics in microalgae. Thus, we investigated the proteins associated with LDs of the emerging heterokont model alga Nannochloropsis sp. and discovered an abundant hydrophobic lipid droplet surface protein (LDSP) with unique primary sequence but structural similarities to other LD proteins. LDSP abundance in Nannochloropsis cells closely tracked the amount of triacylglycerols during conditions of oil accumulation and degradation. Functional characterization of LDSP in an Arabidopsis (Arabidopsis thaliana) OLEOSIN1-deficient mutant allowed a separation of its physical and structural properties in its interaction with LDs from its physiological or biochemical activities. Although LDSP presence in Arabidopsis predictably affected LD size, it could not reverse the physiological impact of OLEOSIN deficiency on triacylglycerol hydrolysis during germination

Remodeling of Membrane Lipids in Iron-starved Chlamydomonas *

Chlamydomonas reinhardtii cells exposed to abiotic stresses (e.g. nitrogen, zinc, or phosphorus deficiency) accumulate triacylglycerols (TAG), which are stored in lipid droplets. Here, we report that iron starvation leads to formation of lipid droplets and accumulation of TAGs. This occurs between 12 and 24 h after the switch to iron-starvation medium. C. reinhardtii cells deprived of iron have more saturated fatty acid (FA), possibly due to the loss of function of FA desaturases, which are iron-requiring enzymes with diiron centers. The abundance of a plastid acyl-ACP desaturase (FAB2) is decreased to the same degree as ferredoxin. Ferredoxin is a substrate of the desaturases and has been previously shown to be a major target of the iron deficiency response. The increase in saturated FA (C16:0 and C18:0) is concomitant with the decrease in unsaturated FA (C16:4, C18:3, or C18:4). This change was gradual for diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) and digalactosyldiacylglycerol (DGDG), whereas the monogalactosyldiacylglycerol (MGDG) FA profile remained stable during the first 12 h, whereas MGDG levels were decreasing over the same period of time. These changes were detectable after only 2 h of iron starvation. On the other hand, DGTS and DGDG contents gradually decreased until a minimum was reached after 24-48 h. RNA-Seq analysis of iron-starved C. reinhardtii cells revealed notable changes in many transcripts coding for enzymes involved in FA metabolism. The mRNA abundances of genes coding for components involved in TAG accumulation (diacylglycerol acyltransferases or major lipid droplet protein) were increased. A more dramatic increase at the transcript level has been observed for many lipases, suggesting that major remodeling of lipid membranes occurs during iron starvation in C. reinhardtii