Instructions for laboratory training in General Microbiology & Immunology for students of medical faculty

МИКРОБИОЛОГИЯАЛЛЕРГОЛОГИЯ И ИММУНОЛОГИЯЛАБОРАТОРНЫЕ РАБОТЫИНОСТРАННЫЕ СТУДЕНТЫПРАКТИЧЕСКИЕ НАВЫКИПРАКТИЧЕСКИЕ ЗАНЯТИЯИНСТРУКЦИИИММУНОЛОГИЯВ издании содержатся план, программа практических занятий и основные практические навыки по общей микробиологии и иммунологии

GAS CHROMATOGRAPHY TECHNIQUE OPTIMIZATION FOR CHECKING RESIDUAL ETHANOL IN THE PREPARATION OF HETEROLOGOUS ANTI-RABIES IMMUNOGLOBULIN

The article presents the results of determination of residual ethanol in the preparation of heterologous antirabies immunoglobulin, using portable photoionization gas chromatograph PGC-1. The advantages of the method are as follows: accuracy, sensitivity, promptness. It does not require sample preparation; the results can be processed automatically

Experimental Substantiation of Cultural Technologies Introduction into Manufacturing of Anti-Rabies Immunoglobulin

The review provides information on the major outcomes of research and development work, performed within the frames of the Federal Target Program “National system of chemical and biological safety” (2009-2014), aimed at elaboration and introduction of cultural techniques into the manufacturing of anti-rabies immunoglobulin. Described are the key phases in methodology engineering, deployed for the large-scale cultivation of fixed rabies virus, concentration of cultural liquid, quantitation of rabies virus using PCR, and immunization of producers. Obtained pilot batches of the enhanced anti-rabies immunoglobulin, complying with normative requirements to commercial formulations, testify to the effectiveness of the developed biotechnological and methodological procedures, as well as of the designed engineering-manufacturing project documentation

Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture

/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for the obtainment of culture liquid with rabies virus titer - 1:256 - 1:512, if assayed in ELISA. This cultural virus is recommended as a basis for immunization material obtainment with a view to produce anti-rabies immunoglobulin from equine blood serum

Classical and cubic Rashba effect in the presence of in-plane 4f magnetism at the iridium silicide surface of the antiferromagnet GdIr2Si2

We present a combined experimental and theoretical study of the two-dimensional electron states at the iridium-silicide surface of the antiferromagnet GdIr2Si2 above and below the Ned temperature. Using angle-resolved photoemission spectroscopy (ARPES) we find a significant spin-orbit splitting of the surface states in the paramagnetic phase. By means of ab initio density-functional-theory (DFT) calculations we establish that the surface electron states that reside in the projected band gap around the (M) over bar point exhibit very different spin structures which are governed by the conventional and the cubic Rashba effect. The latter is reflected in a triple spin winding, i.e., the surface electron spin reveals three complete rotations upon moving once around the constant energy contours. Below the Ned temperature, our ARPES measurements show an intricate photoemission intensity picture characteristic of a complex magnetic domain structure. The orientation of the domains, however, can be clarified from a comparative analysis of the ARPES data and their DFT modeling. To characterize a single magnetic domain picture, we resort to the calculations and scrutinize the interplay of the Rashba spin-orbit coupling field with the in-plane exchange field, provided by the ferromagnetically ordered 4f moments of the near-surface Gd layer

Экспериментальные исследования по изучению эффективности действия препарата «Тарзан, ВЭ» на представителей Blattoptera

The purpose of the research is studying the efficacy of "Tarzan, VE" against representatives of the Blattoptera order, Blattella germanica.Materials and methods. Experimental studies to study the efficacy of "Tarzan, VE" on representatives of the Blattoptera order were performed for two weeks at the premises of the Skryabin MVA (Moscow) and the KemSMU (Kemerovo). The study object was the red German cockroach B. germanica, a laboratory culture of which was grown in the MVA insectarium. Experiments 1 and 2 consisted of three tests of three sets each: a test to study the efficacy of the drug against cockroaches by the topical application; and a test to study the efficacy of "Tarzan, VE" against cockroaches by the forced exposure of arthropods to test surfaces, namely, plywood or glass previously treated with the drug in different concentrations. Dead insects were counted after a day.Results and discussion. We established the efficacy of "Tarzan, VE" in the form of an active substance in different dilutions against cockroaches B. germanica of both sexes using the topical application. Its efficiency decreases to 97% when diluted 1000 times (0.001N). With forced exposure to treated test surface (plywood), the efficacy of “Tarzan, VE” depended on the substance diluted: 90% at 0.01N, 83.3% at 0.005N, and 50% at 0.001N. The maximum effect of "Tarzan, VE" was observed when using the method of forced exposure of cockroaches to the treated test surface, glass. The efficacy of the drug in this case was 100% regardless of the sex and development stage of cockroaches. It was found that different concentrations of the insecticide had a toxic effect on the imago of both sexes and larval stages of cockroach development. The insecticidal nature of the drug is ensured by the use of zeta-cypermethrin as an active ingredient. "Tarzan, VE" insecticide can be recommended to control and prevent the distribution of B. germanica. Цель исследований: изучить эффективность действия препарата «Тарзан, ВЭ» на представителей отряда Таракановых (Blattoptera) – Blattella germanica.Материалы и методы. Экспериментальные исследования по изучению эффективности действия препарата «Тарзан, ВЭ» на представителей отряда Таракановых (Blattoptera) проводили в течение двух недель на базе МВА имени К. И. Скрябина (г. Москва) и на базе КемГМУ (г. Кемерово). В качестве объекта был взят рыжий таракан прусак B. germanica, лабораторная культура которого была выращена в инсектарии МВА. Эксперименты № 1 и 2 состояли из трех опытов по три серии в каждом: опыт по изучению эффективности препарата в отношении тараканов методом топикального нанесения; опыт по изучению эффективности препарата «Тарзан, ВЭ» на тараканов методом принудительных контактов членистоногих с тест-поверхностями – фанерой и стеклом, предварительно обработанных препаратом в разной концентрации. Учет гибели насекомых проводили по истечении суток.Результаты и обсуждение. Установлена эффективность использования «Тарзана, ВЭ» в виде субстанции разной степени разведения против тараканов B. germanica обоего пола при использовании метода топикального нанесения. Эффективность его снижается до 97% при разведении в 1000 раз (0,001N). При принудительном контакте с обработанной тест-поверхностью (фанерой) эффективность «Тарзана, ВЭ» зависела от разведения субстанции: 0,01N – 90%, 0,005N – 83,3, 0,001N – 50%. Максимальный эффект «Тарзана, ВЭ» наблюдали при использовании метода принудительного контакта тараканов с обработанной тест-поверхностью - стеклом. Эффективность препарата в этом случае составила 100% независимо от пола и стадии развития тараканов. Установлено, что разные концентрации инсектицида оказывают токсическое действие на имаго обоего пола и ларвальные стадии развития тараканов. Инсектицидность препарата обеспечивается использованием в качестве действующего вещества зета-циперметрина. Инсектицид «Тарзан, ВЭ» можно рекомендовать для борьбы с B. germanica и профилактики их распространения

Divalent EuRh2Si2 as a reference for the Luttinger theorem and antiferromagnetism in trivalent heavy-fermion YbRh2Si2

Application of the Luttinger theorem to the Kondo lattice YbRh2Si2 suggests that its large 4f-derived Fermi surface (FS) in the paramagnetic (PM) regime should be similar in shape and volume to that of the divalent local-moment antiferromagnet (AFM) EuRh2Si2 in its PM regime. Here we show by angle-resolved photoemission spectroscopy that paramagnetic EuRh2Si2 has a large FS essentially similar to the one seen in YbRh2Si2 down to 1 K. In EuRh2Si2 the onset of AFM order below 24.5 K induces an extensive fragmentation of the FS due to Brillouin zone folding, intersection and resulting hybridization of the Fermi-surface sheets. Our results on EuRh2Si2 indicate that the formation of the AFM state in YbRh2Si2 is very likely also connected with similar changes in the FS, which have to be taken into account in the controversial analysis and discussion of anomalies observed at the quantum critical point in this system

Cultural Antigen in the Technology for Anti-Rabies Immunoglobulin Obtainment from Equine Blood Serum

week after immunization (specific activity is identified using neutralization reaction on the model of white mice and dot-blot immunoassay). This level of activity is sufficient for the fractioning of immune serum and extraction of anti-rabies immunoglobulin. Physicochemical and biological properties of the anti-rabies immunoglobulin, obtained with the help of cultural antigen technique, meet the requirements stated in the normative documentation on anti-rabies immunoglobulins extracted from equine blood serum. Specific activity level of experimental batches of anti-rabies immunoglobulin, obtained with the help of cultural technologies, corresponds to 242 and 214 IU/ml

Production of Rabbit Anti-Rabies Immunoglobulin Using Cultural Antigen

Justified is the possibility of application of fixed rabies virus Moscow 3253, reproduced on the cell culture Vero, as an antigen for heterologous anti-rabies immunoglobulin production. Application of the adjuvant - aluminium hydrate - is determined to be effective. Herein, by the day 73 post immunization, specific antibodies titer is ≥ 1:500 (wherein specific activity has been identified by means of neutralization test, carried out on white mice, and dot-blot immunoassay). The level of specific activity in experimental samples of anti-rabies immunoglobulin, isolated from rabbit immune serum, corresponds to 332 and 347 ME/ml. Physicochemical and biological properties of anti-rabies immunoglobulin, produced with the help of cultural antigen, fully comply with regulatory requirements specified for commercial preparation of heterologous anti-rabies immunoglobulin

ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN «MOSCOW 3253», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS

Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay