Metodología para evaluar la relación entre la ecoeficiencia empresarial y el desarrollo local sostenible

El objetivo del trabajo fue diseñar una metodología para evaluar bidireccionalmente la relación entre la ecoeficiencia empresarial y el desarrollo local sostenible. Se definieron las etapas del procedimiento propuesto: primero, evaluación de la contribución de la ecoeficiencia empresarial al desarrollo local sostenible; segundo, evaluación de la contribución de los actores locales a la ecoeficiencia empresarial; y tercero, diseño del curso de acciones encaminadas al perfeccionamiento de la relación ecoeficiencia empresarial-desarrollo local sostenible

La reforma tributaria cubana: evolución, peculiaridades y perspectivas

Se analiza la reforma tributaria cubana considerando su evolución histórica, con énfasis en sus peculiaridades y transformaciones estructurales actuales. En el sistema tributario cubano, por ser parte del sistema socialista, coexisten formas de gestión estatal y no estatal, con predominio de la primera, y además existe la peculiaridad de que todos los ciudadanos se benefician por igual de todos los servicios públicos. Su principal función: la recaudación de recursos financieros, se complementa con la política educativa de que toda la población contribuya al equilibrio de las cuentas fiscales. El principal reto es que, además de generar eficiencia en las empresas estatales, deberá ser un potente mecanismo para la redistribución de ingresos en la población, paleando la desigualdad social. Una mejor comprensión de la importancia y funcionamiento del sistema tributario producirá un efecto positivo y un resultado significativo para lasociedad en su conjunto

Patrón fatiga-recuperación en una competición de alta densidad competitiva en Baloncesto Femenino Junior

El objetivo principal del trabajo fue analizar la fatiga y recuperación, mediante dos métodos de aproximación diferentes pero relacionados entre ellos; la carga interna y la carga externa. Los sujetos estudiados fueron el equipo Junior del C.B. Conquero, conformado éste por 12 jugadoras con edades comprendidas entre 16 y 18 años (M=17.08; SD=.67). Los datos se recolectaron durante el Campeonato de Andalucía Base de Baloncesto Femenino, celebrado en la temporada 2015–2016. Se estudiaron diferentes variables en cuanto carga interna como percepción subjetiva del esfuerzo, calidad de la recuperación, bienestar percibido y la valoración subjetiva del rendimiento post-partido. En cuanto a las variables de carga externa, se estudió la cantidad de minutos de juego de cada jugadora y la altura de salto. Los resultados principales indicaron que el bienestar percibido podría ser explicado por la calidad de la recuperación. Por otra parte, la valoración del rendimiento post partido podía ser explicada por el tiempo jugado y por la altura alcanzada en el test de salto. Se podría decir que las herramientas utilizadas, son válidas para monitorizar la fatiga y recuperación en un periodo de alta densidad competitiva en jugadoras juveniles.

Relación entre la complejidad técnico-táctica en la sesión de entrenamiento y la carga interna en baloncesto femenino

The objective of this work was to analyze the rating scale load-Tactical Technical (Coque, 2009) Training and Its Relationship with internal load parameters. Currently, a planning model characterized by concern to improve the interpretation that the subject does what happens around them during the competition (Martin Steel, Seirul-lo Vargas, Lake Peñas, and Novoa Lalin arises, 2013), to modify the response and in turn, the drive scheme. The study involved the players formed Women's League team Basketball Club Conquero, composed of 12 players, aged between 17 and 33 years (M = 21.91, SD = 4.81). Data were collected during the last two months of competition in February and March. Par obtain the data necessary the following scales of subjective assessment of the burden of technical and tactical training (Coque, 2009), perceived exertion (Borg, 1970; 1998; Borg & Kaijser,2006) and quality of recovery (Kentta & Hassmen, 1998) were used. The results showed that only the density of training is the only marker that could explain significantly (91.4%) perceived effort of the players. According to data from the perceived exertion, it could be used for monitoring training regarding technical and tactical complexity.El objetivo de este trabajo fue analizar la Escala de Valoración de la Carga del entrenamiento Técnico-Táctico (Coque, 2009) y su relación con parámetros de carga interna. En la actualidad, se plantea un modelo de planificación que se caracteriza por la preocupación de mejorar la interpretación que el sujeto hace de lo que sucede a su alrededor durante la competición (Martín Acero, Seirul-lo Vargas, Lago Peñas, y Novoa Lalín, 2013), para así modificar la respuesta y a su vez, el esquema motriz. El estudio contó con las jugadoras del equipo de Liga Femenina del Club Baloncesto Conquero, compuesto por 12 jugadoras, con edades comprendidas entre 17 y 33 años (M = 21.91; SD = 4.81). Los datos fueron recogidos durante los dos últimos meses de competición que se corresponden con los meses de febrero y marzo de 2016. Para obtener los datos necesarios se utilizaron la escala de valoración subjetiva de la carga de entrenamiento Técnico-Táctico  (Coque, 2009), la percepción subjetiva del esfuerzo (Borg, 1970; 1998; Borg & Kaijser,2006) y la calidad de la recuperación  (Kentta & Hassmen, 1998). Los resultados mostraron que sólo la densidad del entrenamiento es el único marcador que podría explicar, de forma importante (91.4%), el esfuerzo percibido de las jugadoras. Según los datos obtenidos la percepción subjetiva del esfuerzo podría ser utilizada para la monitorización del entrenamiento en cuanto a la complejidad técnico-táctica del mismo

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. One hypothesis is that the extracellular compounds produced by the fungi associated with grapevine trunk diseases are responsible for pathogenicity. In the present work, we used Vitis vinifera cv. Chardonnay cells to test the aggressiveness of total extracellular compounds produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with botryosphaeria dieback. Additionally, the toxicity of purified mellein, a characteristic toxin present in the extracellular compounds of Botryosphaeriaceae, was assessed. Our results show that the total extracellular compounds produced by N. parvum induce more necrosis on Chardonnay calli and induce a different defence gene expression pattern than those of D. seriata. Mellein was produced by both fungi in amounts proportional to its aggressiveness. However, when purified mellein was added to the culture medium of calli, only a delayed necrosis and a lower-level expression of defence genes were observed. Extracellular compounds seem to be involved in the pathogenicity of the fungi associated with botryosphaeria dieback. However, the doses of mellein used in this study are 100 times higher than those found in the liquid fungal cultures: therefore, the possible function of this toxin is discussed

“Determinantes de la incorporación precoz de alimentos en niños menores de 6 meses de edad que asisten a los Centros de Atención Primaria de la Salud de Zona Sanitaria I Central, 2012”.

La necesidad de una adecuada alimentación complementaria en la infancia conduce a evaluar posibles factores determinantes de la incorporación precoz dealimentos en niños menores de 6 meses de edad y su estado nutricional, de todos los Centros de Atención Primaria de la Salud de la Zona Sanitaria I Central, 2012. El trabajopropuso caracterizar población, contexto familiar y contexto socio-cultural; determinar tipo y calidad de información de las madres y evaluar nutricionalmente a niños menoresde 12 meses de edad. Se realizaron encuestas a madres en edad fértil que asisten periódicamente a los CAPS de la Zona Sanitaria I Central. Valoración del estado nutricional a los respectivos hijos menores de 12 meses de edad

Collaborating around digital tabletops: children’s physical strategies from the UK, India and Finland

We present a study of children collaborating around interactive tabletops in three different countries: the United Kingdom, India and Finland. Our data highlights the key distinctive physical strategies used by children when performing collaborative tasks during this study. Children in the UK tend to prefer static positioning with minimal physical contact and simultaneous object movement. Children in India employed dynamic positioning with frequent physical contact and simultaneous object movement. Children in Finland used a mixture of dynamic and static positioning with minimal physical contact and object movement. Our findings indicate the importance of understanding collaboration strategies and behaviours when designing and deploying interactive tabletops in heterogeneous educational environments. We conclude with a discussion on how designers of tabletops for schools can provide opportunities for children in different countries to define and shape their own collaboration strategies for small group learning that take into account their different classroom practices

A protein functionalization platform based on selective reactions at methionine residues.

Nature has a remarkable ability to carry out site-selective post-translational modification of proteins, therefore enabling a marked increase in their functional diversity1. Inspired by this, chemical tools have been developed for the synthetic manipulation of protein structure and function, and have become essential to the continued advancement of chemical biology, molecular biology and medicine. However, the number of chemical transformations that are suitable for effective protein functionalization is limited, because the stringent demands inherent to biological systems preclude the applicability of many potential processes2. These chemical transformations often need to be selective at a single site on a protein, proceed with very fast reaction rates, operate under biologically ambient conditions and should provide homogeneous products with near-perfect conversion2-7. Although many bioconjugation methods exist at cysteine, lysine and tyrosine, a method targeting a less-explored amino acid would considerably expand the protein functionalization toolbox. Here we report the development of a multifaceted approach to protein functionalization based on chemoselective labelling at methionine residues. By exploiting the electrophilic reactivity of a bespoke hypervalent iodine reagent, the S-Me group in the side chain of methionine can be targeted. The bioconjugation reaction is fast, selective, operates at low-micromolar concentrations and is complementary to existing bioconjugation strategies. Moreover, it produces a protein conjugate that is itself a high-energy intermediate with reactive properties and can serve as a platform for the development of secondary, visible-light-mediated bioorthogonal protein functionalization processes. The merger of these approaches provides a versatile platform for the development of distinct transformations that deliver information-rich protein conjugates directly from the native biomacromolecules

Survivorship and Clinical Outcome of the Minimally Invasive Uniglide Medial Fixed Bearing, All-polyethylene Tibia, Unicompartmental Knee Arthroplasty at a Mean Follow-up of 7.3 Years

Background: Medial UKA performed in England and Wales represents 7 to 11% of all knee arthroplasty procedures, and is most commonly performed using mobile-bearing designs. Fixed bearing eliminates the risk of bearing dislocation, however some studies have shown higher revision rates for all-polyethylene tibial components compared to those that utilize metal-backed implants. The aim of the study is to analyse survivorship and maximum 8-year clinical outcome of medial fixed bearing, Uniglide unicompartmental knee arthroplasty performed using an all-polyethylene tibial component with a minimal invasive approach. Methods: Between 2002 and 2009, 270 medial fixed UKAs were performed in our unit. Patients were reviewed pre-operatively, 5 and 8 years post-operatively. Clinical and radiographic reviews were carried out. Patients’ outcome scores (Oxford, WOMAC and American Knee Score) were documented in our database and analysed. Results: Survival and clinical outcome data of 236 knees with a mean 7.3 years follow-up are reported. Every patient with less than 4.93 years follow-up underwent a revision. The patients’ average age at the time of surgery was 69.5 years. The American Knee Society Pain and Function scores, the Oxford Knee Score and the WOMAC score all improved significantly. The 5 years survival rate was 94.1% with implant revision surgery as an end point. The estimated 10 years survival rate is 91.3%. 14 patients were revised before the 5 year follow-up. Conclusion: Fixed bearing Uniglide UKA with an all-polyethylene tibial component is a valuable tool in the management of a medial compartment osteoarthritis, affording good short term survivorship