Control of Ralstonia Solanacearum Infection in Tomato, Brinjal and Capsicum by Antibiotic Sensitivity Test

Ralstonia solanacearum is one of the important dreaded soil's borne bacterial phytopathogen which causes enormous losses in the crop plants in tropical, subtropical and temperate region of the world. In India, the disease is highly prevalent and active throughout the year where the soil is acidic in the Eastern Plateau and Hill Region. Once the disease is established in the field, it cannot be controlled by chemical means. Keeping these in view, screening of potential antibiotics for the management of R. solanacearum was done. In the present study, three strains of R. solanacearum were isolated from Brinjal, Tomato and Capsicum. Against these three strains of R. solanacearum, four antibiotics were screened through food poison techniques viz. Kasugamycin, Streptomycin, Ceftriaxone and Gentamicin. The different strains of R. solanacearum and antibiotic sensitivity showed varied response. Among the screened antibiotics, Gentamicin showed strong antibacterial efficacy which inhibited 100% Colony-forming Unit (cfu) at very low concentration (1 ppm) followed by Ceftriaxone which inhibited >50% cfu at 1 ppm against all three strains. However, Streptomycin also showed antibacterial efficacy and inhibited >50% cfu at 3 ppm, but Kasugamycin was found less antibacterial as compared to other tested antibiotics and inhibited >50% cfu at 4 ppm

Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test

Ralstonia solanacearum is one of the important dreaded soil’s borne bacterial phytopathogen which causes enormous losses in the crop plants in tropical, subtropical and temperate region of the world. In India, the disease is highly prevalent and active throughout the year where the soil is acidic in the Eastern Plateau and Hill Region. Once the disease is established in the field, it cannot be controlled by chemical means. Keeping these in view, screening of potential antibiotics for the management of R. solanacearum was done. In the present study, three strains of R. solanacearum were isolated from Brinjal, Tomato and Capsicum. Against these three strains of R. solanacearum, four antibiotics were screened through food poison techniques viz. Kasugamycin, Streptomycin, Ceftriaxone and Gentamicin. The different strains of R. solanacearum and antibiotic sensitivity showed varied response. Among the screened antibiotics, Gentamicin showed strong antibacterial efficacy which inhibited 100% Colony-forming Unit (cfu) at very low concentration (1 ppm) followed by Ceftriaxone which inhibited >50% cfu at 1 ppm against all three strains. However, Streptomycin also showed antibacterial efficacy and inhibited >50% cfu at 3 ppm, but Kasugamycin was found less antibacterial as compared to other tested antibiotics and inhibited >50% cfu at 4 ppm

Trichoderma asperellum, a potential fungal biocontrol agent against Aspergillus niger

Trichoderma asperellum is free-living, ubiquitous fungus which is very common in the habitat of soil and root ecosystem, is known as a potent opportunistic, avirulent plant symbionts and it parasitizes several soilborne phytopathogens. Aspergillus niger is well known black mold which causes several storage diseases. Among the storage diseases, black mould disease of onion is an important disease which is caused by A. niger. Antagonsitic potential of T. asperellum was assayed against three isolates of A. niger [RC1, RC2 (isolated from soil samples of Farm 1 and 2) and RC3 isolated from diseases onion]. Antagonistic efficacy of T. asperellum of A. niger almost similar against all the test isolates. Percentage inhibition of radial growth (PIRG) of A. niger by T. asperellum inhibited 55.17% within five days, 77.20% within 7 days and 92.06% in 12 days. Antagonistic efficacy of T. asperellum can be exploited in the management of black mould disease of onion

Metabolomics-Driven Mining of Metabolite Resources:Applications and Prospects for Improving Vegetable Crops

Vegetable crops possess a prominent nutri-metabolite pool that not only contributes to the crop performance in the fields, but also offers nutritional security for humans. In the pursuit of identifying, quantifying and functionally characterizing the cellular metabolome pool, biomolecule separation technologies, data acquisition platforms, chemical libraries, bioinformatics tools, databases and visualization techniques have come to play significant role. High-throughput metabolomics unravels structurally diverse nutrition-rich metabolites and their entangled interactions in vegetable plants. It has helped to link identified phytometabolites with unique phenotypic traits, nutri-functional characters, defense mechanisms and crop productivity. In this study, we explore mining diverse metabolites, localizing cellular metabolic pathways, classifying functional biomolecules and establishing linkages between metabolic fluxes and genomic regulations, using comprehensive metabolomics deciphers of the plant’s performance in the environment. We discuss exemplary reports covering the implications of metabolomics, addressing metabolic changes in vegetable plants during crop domestication, stage-dependent growth, fruit development, nutri-metabolic capabilities, climatic impacts, plant-microbe-pest interactions and anthropogenic activities. Efforts leading to identify biomarker metabolites, candidate proteins and the genes responsible for plant health, defense mechanisms and nutri-rich crop produce are documented. With the insights on metabolite-QTL (mQTL) driven genetic architecture, molecular breeding in vegetable crops can be revolutionized for developing better nutritional capabilities, improved tolerance against diseases/pests and enhanced climate resilience in plants

Measurement of the Higgs boson production rate in association with top quarks in final states with electrons, muons, and hadronically decaying tau leptons at s√=13TeV

The rate for Higgs (H) bosons production in association with either one (tH) or two (tt¯H) top quarks is measured in final states containing multiple electrons, muons, or tau leptons decaying to hadrons and a neutrino, using proton–proton collisions recorded at a center-of-mass energy of 13TeV by the CMS experiment. The analyzed data correspond to an integrated luminosity of 137fb−1. The analysis is aimed at events that contain H→WW, H→ττ, or H→ZZ decays and each of the top quark(s) decays either to lepton+jets or all-jet channels. Sensitivity to signal is maximized by including ten signatures in the analysis, depending on the lepton multiplicity. The separation among tH, tt¯H, and the backgrounds is enhanced through machine-learning techniques and matrix-element methods. The measured production rates for the tt¯H and tH signals correspond to 0.92±0.19(stat)+0.17−0.13(syst) and 5.7±2.7(stat)±3.0(syst) of their respective standard model (SM) expectations. The corresponding observed (expected) significance amounts to 4.7 (5.2) standard deviations for tt¯H, and to 1.4 (0.3) for tH production. Assuming that the Higgs boson coupling to the tau lepton is equal in strength to its expectation in the SM, the coupling yt of the Higgs boson to the top quark divided by its SM expectation, κt=yt/ySMt, is constrained to be within −0.9<κt<−0.7 or 0.7<κt<1.1, at 95% confidence level. This result is the most sensitive measurement of the tt¯H production rate to date.SCOAP

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Not AvailableA natural red dye which is produced by the tiny insects Kerria lacca while feeding on host trees is popularly known as lac dye. Lac dye is a mixture of at least five closely related pure compounds all being anthraquinone derivatives designated as laccaic acid A, B, C, D and E. Anthraquinones isolated from different natural sources and reported to have potent antimicrobial activity. The lac dye, which is also a mixture of anthraquinone derivatives, is expected to exhibit antifungal and antibacterial activity. Lac dye cannot be used as antibacterial and antifungal agent due to its low water solubility and high polarity. Therefore, it is modified into its methyl derivative to enhance its bio-efficacy. Methylated lac dye is characterized with the help of TLC, UV–Vis spectroscopy and FT-IR, NMR analysis. An in vitro spore germination assay was carried out to evaluate the antifungal efficacy of methylated lac dye against some phytopathogenic fungi which commonly caused a various foliar diseases in crop plants viz., Alternaria solani, Curvularia lunata, Erysiphe pisi, Helminthosporium oryzae and Verticillium sp. Among the tested fungi, Verticillum sp. showed highest sensitivity, which showed 100% inhibition at 750 and 1000 lg/ml as compared to control. However, E. pisi an obligate parasite also showed varied sensitivity but at 1000 lg/ml showed 100% spore germination as compared to control. Methylated lac dye also showed strong antibacterial properties against Ralstonia solanacearum at very low concentration (40 and 50 lg/ml). Hence, lac dye may serve as potent antifungal and antibacterial agent in plant disease management.Not Availabl

The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens

Abstract The antagonistic potential of Trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of Sclerotium rolfsii, the causal agent of chickpea collar rot. Trichoderma harzianum-1432 (42.2%) and Trichoderma atroviride (40.3%) were found to be strong antagonists. To enhance their antagonistic potential, mutagenesis of these two selected strains was performed. Two mutants, Th-m1 and T. atroviride m1, were found to be more effective than their parent strains. The enzymatic activities of the selected parent and mutant strains were assayed, and although both mutants were found to have enhanced enzymatic activities compared to their respective parent strains, Th-m1 possessed the maximum cellulase (5.69 U/mL) and &#946;-1,3-glucanase activity (61.9 U/mL). Th-m1 also showed high competitive saprophytic ability (CSA) among all of the selected parent and mutant strains, and during field experiments, Th-m1 was found to successfully possess enhanced disease control (82.9%)

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Not AvailableLitchi stink bug, LSB (Tessaratoma javanica) is a major insect pest of litchi in India and insecticidal control of this pest is non effective. Therefore, in present study, exploration of natural enemies of litchi stink bug and their effect on pest population was investigated for two years during 2012 and 2013. The LSB females were found to start laying eggs from February and peak activity was recorded in the month of March to April during both years. Three egg parasitoids of litchi stink bug were recorded, two were from the family Eupelmidae and one was from the family Encyrtidae of order Hymenoptera. Among parasitoids recorded, only two were identified up to species level namely Anastatus bangalorensis and Anastatus acherontiae. We are reporting these two parasitoids as new record from litchi stink bug (T. javanica). Maximum parasitized eggs were found in the month of March with 42.54% and 46.12%, respectively in insecticides free litchi orchard during 2012 and 2013 with major parasitization by A. bangalorensis. The egg parasitoids of litchi stink bug would be beneficial in the integrated management of the pest if mass reared and released in the litchi orchards.Not Availabl

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Not AvailableThe aim of the present study was to identify the cultivable gut bacteria associated with peach fruit fly, Bactrocera zonata, and evaluate their potential to attract adults of B. zonata. Based on culture-dependent characterization methods and 16S rRNA gene sequence analysis, bacteria were identified as members of family Enterobacteriaceae (BZM1, Klebsiella oxytoca), Microbacteriacea (BZM2, Microbacterium spp.) and Nocardiaceae (BZM4, Rhodococcus spp.). Molecular phylogeny placed Klebsiella oxytoca within gram negative γ-proteobacteria whereas, Microbacterium spp. and Rhodococcus spp. were clustered under gram positive Actinobacteria group in family Microbacteriacea and Nocardiaceae, respectively. 16S rRNA gene sequence comparison with the available NCBI database sequences further confirmed the characterizations of bacterial symbionts. Population of these bacterial species increased significantly up to the 11th day after emergence of adults and thereafter it remains constant. Among 3 bacterial symbionts, metabolites produced from K. oxytoca had the highest attraction to the B. zonata adult females over metabolites produced from other bacteria and their combinations in field bioassay. The B. zonata adult male flies attracted to metabolites produced from each bacterial symbionts alone and their combinations were less in number with comparison to the B. zonata adult females. The present study provides the first description of the attractancy potential of metabolites produced by gut microbial community of B. zonata in open field condition. This study results may prompt the development of a female-targeted population control strategy for this fly.Not Availabl