A FORMAÇÃO DE EDUCADORES ATRAVÉS DE PERCURSOS ESTÉTICOS: TEATRO,MUSICA E NARRAÇÃO

Este trabalho apresenta e problematiza percursos de pesquisa-ação que estamos experimentando no 'Laboratorio di Epistemologia e pratiche dell'educazione' (LEPE) - Universidade Federico II de Nápoles (UNINA) e do Projeto de Extensão 'Educação Popular no LUAR' - Universidade Federal de Rio de Janeiro (UFRJ). Estamos criando percursos que partem da experiência biográfica e narrativa focalizando elementos relacionados com a educação através de uma analise sincrônica e comparativa sobre experiências sociais e políticas

Use of DPP4 inhibitors in Italy does not correlate with diabetes prevalence among COVID-19 deaths

In a nationwide study of 3,818 charts from patients with fatal COVID-19, we found that geographical differences in Dipeptidyl peptidase 4 (DPP4) inhibitors use did not correlate with diabetes prevalence among COVID-19 deaths, thus not supporting the hypothesis of a clinically relevant involvement of DPP4 in COVID-19 development and progression

In vitro effects of simulated microgravity on Sertoli cell function

With the advent of space flights questions concerning the effects of microgravity (0G) on human reproductive physiology have received great attention. The aim of this study was to evaluate the influence of 0G on Sertoli cells. A Sertoli cell line from mouse testis (42GPA9) was analyzed for cytoskeletal and Sex Hormone Binding Globilin (SHBG) changes by immunohistochemistry, for antioxidant content by RT-PCR and for culture medium lactate concentrations by protein chemistry. Cells were cultured for 6, 24 and 48 h on a three-dimensional Random Positioning Machine (3D-RPM); static controls (1G) were positioned on the supporting frame. At the end of each experiment, cultured cells were either fixed in paraformaldehyde or lysed and RNA-extracted or used for culture medium lactate measurements as needed. At 0G, Sertoli cytoskeleton became disorganized, microtubules fragmented and SHBG undetectable already after 24 h, with alterations worsening by 48 h. It was evident that various antioxidant systems appreciably increased during the first 24 h but significantly decreased at 48 h. No changes occurred in the 1G samples. Initially, 0G seemed to disturb antioxidant pro- tection strategies allowing the testes to support sperm production, thus generating an aging-like state of oxidative stress. Lactate pro- duction at 0G slightly decreased after 24 h. Further experiments are needed in space to investigate upon steroidogenesis and germ cell differentiation within the testis, to rule out male infertility as a possible consequence, which could be a problem, as life expectancy increases.With the advent of space flights questions concerning the effects of microgravity (0G) on human reproductive physiology have 13 received great attention. The aim of this study was to evaluate the influence of 0G on Sertoli cells. A Sertoli cell line from mouse testis 14 (42GPA9) was analyzed for cytoskeletal and Sex Hormone Binding Globilin (SHBG) changes by immunohistochemistry, for antioxidant 15 content by RT-PCR and for culture medium lactate concentrations by protein chemistry. Cells were cultured for 6, 24 and 48 h on a 16 three-dimensional Random Positioning Machine (3D-RPM); static controls (1G) were positioned on the supporting frame. At the 17 end of each experiment, cultured cells were either fixed in paraformaldehyde or lysed and RNA-extracted or used for culture medium 18 lactate measurements as needed. At 0G, Sertoli cytoskeleton became disorganized, microtubules fragmented and SHBG undetectable 19 already after 24 h, with alterations worsening by 48 h. It was evident that various antioxidant systems appreciably increased during the 20 first 24 h but significantly decreased at 48 h. No changes occurred in the 1G samples. Initially, 0G seemed to disturb antioxidant pro- 21 tection strategies allowing the testes to support sperm production, thus generating an aging-like state of oxidative stress. Lactate pro- 22 duction at 0G slightly decreased after 24 h. Further experiments are needed in space to investigate upon steroidogenesis and germ 23 cell differentiation within the testis, to rule out male infertility as a possible consequence, which could be a problem, as life expectancy 24 increase

Body composition and metabolic changes during a 520\u2011day mission simulation to Mars

Purpose The \u201cMars-500 project\u201d allowed to evaluate the changes in psychological/physiological adaptation over a prolonged confinement, in order to gather information for future missions. Here, we evaluated the impact of confinement and isolation on body composition, glucose metabolism/insulin resistance and adipokine levels. Methods The \u201cMars-500 project\u201d consisted of 520 consecutive days of confinement from June 3, 2010 to Nov 4, 2011. The crew was composed of six male subjects (three Russians, two Europeans, and one Chinese) with a median age of 31 years (range 27\u201338 years). Results During the 520-day confinement, total body mass and BMI progressively decreased, reaching a significant difference at the end (417 days) of the observation period ( 12 9.2 and 12 5.5%, respectively). Fat mass remained unchanged. A progressive and significant increase of fasting plasma glucose was observed between 249 and 417 days (+ 10/+ 17% vs baseline), with a further increase at the end of confinement (up to + 30%). Median plasma insulin showed a non-significant early increment (60 days; + 86%). Total adiponectin halved ( 12 47%) 60 days after hatch closure, remaining at this nadir ( 12 51%) level for a further 60 days. High molecular weight adiponectin remained significantly lower from 60 to 168 days. Conclusions Based on these data, countermeasures may be envisioned to balance the potentially harmful effects of prolonged confinement, including a better exercise program, with accurate monitoring of (1) the individual activity and (2) the relationship between body composition and metabolic derangement

Site effect assessment in Bishkek (Kyrgyzstan) using earthquake and noise recording data

Kyrgyzstan, which is located in the collision zone between the Eurasian and Indo-Australian lithosphere plates, is prone to large earthquakes as shown by its historical seismicity. Hence, an increase in the knowledge and awareness by local authorities and decision makers of the possible consequence of a large earthquake, based on improved seismic hazard assessments and realistic earthquake risk scenarios, is mandatory to mitigate the effects of an earthquake. To this regard, the Central Asia Cross-Border Natural Disaster Prevention (CASCADE) project aims to install a cross- border seismological and strong motion network in Central Asia and to support microzonation activities for the capitals of Kyrgyzstan, Uzbekistan, Kazakhstan, Tajikistan, and Turkmenistan. During the first phase of the project, a temporary seismological network of 19 stations was installed in the city of Bishkek, the capital of Kyrgyzstan. Moreover, single-station noise recordings were collected at nearly 200 sites. In this study, the site amplifications occurring in Bishkek are assessed by analyzing 56 earthquakes extracted from the data streams continuously acquired by the network, as well as from the single-station noise measurements. A broadband amplification (starting at ∼0:1 and 0.2 Hz), is shown by the standard spectral ratio (SSR) results of the stations located within the basin. The reliability of the observed low-frequency amplification was validated through a time–frequency analysis of denoised seismograms. Discrepancies between horizontal-to-vertical spectral ratio and SSR results are due to the large amplification of the vertical component of ground motion, probably due to the effect of converted waves. The single-station noise results, once their reliability was assessed by their comparison with the earthquake data, have been used to produce the first fundamental resonance frequency map for Bishkek, whose spatial variation shows a good agreement with the presence of an impedance contrast within the Tertiary sedimentary cover.Published3068-30824.1. Metodologie sismologiche per l'ingegneria sismicaJCR Journalope

Positive energy balance is associated with accelerated muscle atrophy and increased erythrocyte glutathione turnover during 5 wk of bed rest

Background: Physical inactivity is often associated with positive energy balance and fat gain. Objective: We aimed to assess whether energy intake in excess of requirement activates systemic inflammation and antioxidant defenses and accelerates muscle atrophy induced by inactivity. Design: Nineteen healthy male volunteers were studied before and at the end of 5 wk of bed rest. Subjects were allowed to spontaneously adapt to decreased energy requirement (study A, n = 10) or were provided with an activity-matched diet (study B, n = 9). Groups with higher (HEB) or lower (LEB) energy balance were identified according to median values of inactivity-induced changes in fat mass (\u394FM, assessed by bioelectrical impedance analysis). Results: In pooled subjects (n = 19; median \u394FM: 1.4 kg), bed rest-mediated decreases in fat-free mass (bioelectrical impedance analysis) and vastus lateralis thickness (ultrasound imaging) were significantly greater (P < 0.03) in HEBAB (-3.8 \ub1 0.4kg and -0.32 \ub1 0.04 cm, respectively) than in LEBab (-2.3 \ub1 0.5 kg and -0.09 \ub1 0.04 cm, respectively) subjects. In study A (median \u394FM: 1.8 kg), bed rest-mediated increases in plasma leptin, C-reactive protein, and myeloperoxidase were greater (P < 0.04) in HEBA than in LEBA subjects. Bed rest-mediated changes of glutathione synthesis rate in eythrocytes (L-[3,3-2H2]cysteine incorporation) were greater (P = 0.03) in HEBA (from 70 \ub1 19 to 164 \ub1 29%/d) than in LEBA (from 103 \ub1 23 to 84 \ub1 27%/d) subjects. Conclusions: Positive energy balance during inactivity is associated with greater muscle atrophy and with activation of systemic inflammation and of antioxidant defenses. Optimizing caloric intake may be a useful strategy for mitigating muscle loss during period of chronic inactivity

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Objective. This study examined the association between obstructive sleep apnea (OSA), daytime sleepiness, functional activity, and objective physical activity. Setting. Subjects (N = 37) being evaluated for OSA were recruited from a sleep clinic. Participants. The sample was balanced by gender (53% male), middle-aged, primarily White, and overweight or obese with a mean BMI of 33.98 (SD = 7.35; median BMI = 32.30). Over 40% reported subjective sleepiness (Epworth Sleepiness Scale (ESS) ≥10) and had OSA (78% with apnea + hypopnea index (AHI) ≥5/hr). Measurements. Evaluation included questionnaires to evaluate subjective sleepiness (ESS) and functional outcomes (Functional Outcomes of Sleep Questionnaire (FOSQ)), an activity monitor, and an overnight sleep study to determine OSA severity. Results. Increased subjective sleepiness was significantly associated with lower scores on the FOSQ but not with average number of steps walked per day. A multiple regression analysis showed that higher AHI values were significantly associated with lower average number of steps walked per day after controlling patient's age, sex, and ESS. Conclusion. Subjective sleepiness was associated with perceived difficulty in activity but not with objectively measured activity. However, OSA severity was associated with decreased objective physical activity in aging adults

Site effect assessment in Bishkek (Kyrgyzstan) using earthquake and noise recording data

Kyrgyzstan, which is located in the collision zone between the Eurasian and Indo-Australian lithosphere plates, is prone to large earthquakes as shown by its historical seismicity. Hence, an increase in the knowledge and awareness by local authorities and decision makers of the possible consequence of a large earthquake, based on improved seismic hazard assessments and realistic earthquake risk scenarios, is mandatory to mitigate the effects of an earthquake. To this regard, the Central Asia Cross-Border Natural Disaster Prevention (CASCADE) project aims to install a cross- border seismological and strong motion network in Central Asia and to support microzonation activities for the capitals of Kyrgyzstan, Uzbekistan, Kazakhstan, Tajikistan, and Turkmenistan. During the first phase of the project, a temporary seismological network of 19 stations was installed in the city of Bishkek, the capital of Kyrgyzstan. Moreover, single-station noise recordings were collected at nearly 200 sites. In this study, the site amplifications occurring in Bishkek are assessed by analyzing 56 earthquakes extracted from the data streams continuously acquired by the network, as well as from the single-station noise measurements. A broadband amplification (starting at ∼0:1 and 0.2 Hz), is shown by the standard spectral ratio (SSR) results of the stations located within the basin. The reliability of the observed low-frequency amplification was validated through a time–frequency analysis of denoised seismograms. Discrepancies between horizontal-to-vertical spectral ratio and SSR results are due to the large amplification of the vertical component of ground motion, probably due to the effect of converted waves. The single-station noise results, once their reliability was assessed by their comparison with the earthquake data, have been used to produce the first fundamental resonance frequency map for Bishkek, whose spatial variation shows a good agreement with the presence of an impedance contrast within the Tertiary sedimentary cover

Objective and subjective sleep disorders in automated peritoneal dialysis

Background: Automated peritoneal dialysis (APD) is one of the fastest growing dialysis modalities. It is unknown whether sleep and mood are disturbed while performing repeated overnight exchanges. Objectives: In this report, we aim to describe and compare the prevalence of sleep-disordered breathing (SDB), periodic limb movements (PLMS), poor sleep quality (SQ), and depression among APD patients compared with stages 3b-5 (estimated glomerular filtration rate ≤44 ml/min/1.73 m2) chronic kidney disease (CKD) and hemodialysis (HD) patients. Design: This is a cross-sectional, descriptive study. Setting: Study participants were recruited from outpatient nephrology clinics, local dialysis centers, and the Thomas E. Starzl Transplant Institute in Western Pennsylvania between April 2004 and July 2009. Patients: There were 186 participants in this study including 22 APD patients, 89 CKD patients, and 75 HD patients. Measurements: In-home polysomnography was performed and two questionnaires were completed, the Pittsburgh Sleep Quality Index (PSQI) and the Patient Health Questionnaire-9 (PHQ-9). Methods: SDB and PLMS were quantified by in-home unattended polysomnography; poor SQ was defined by a score >5 on the PSQI, and the presence of moderate to severe depression was defined by a score >5 on the PHQ-9. Results: The APD patients had a median age of 37.5 years, were predominantly female (72.7 %), and had a median body mass index (BMI) of 23.8 kg/m2. In univariate analyses, APD patients had significantly lower apnea-hypopnea index compared to HD patients by 12.2 points (likelihood ratio test p = 0.008) and revealed the least percent of TST with nocturnal hypoxemia compared to CKD patients by 2.7 points, respectively (likelihood ratio test p = 0.01). The APD group had also significantly greater stages 3 to 4 sleep compared to the CKD patients by 8.6 points (likelihood ratio test p = 0.009). In multivariate analyses and after adjustment for age, gender, race, and BMI, both APD and HD patients had higher average PSQI scores than CKD patients by 2.54 and 2.22 points, respectively (likelihood ratio test p = 0.005). No other comparisons of sleep parameters among groups reached statistical significance. Limitations: The limitations of this study are the small sample size of the APD population and the demographic and clinical differences among the three study groups. Conclusions: Despite differences in univariate analyses, after multivariate adjustment, APD patients had similar sleep parameters and sleep architecture and as poor SQ and symptoms of depression as HD patients. Future studies with larger APD cohorts are needed

Autoantibodies to posttranslational modifications in rheumatoid arthritis

Autoantibodies have been associated with human pathologies for a long time, particularly with autoimmune diseases (AIDs). Rheumatoid factor (RF) is known since the late 1930s to be associated with rheumatoid arthritis (RA). The discovery of anticitrullinated protein antibodies in the last century has changed this and other posttranslational modifications (PTM) relevant to RA have since been described. Such PTM introduce neoepitopes in proteins that can generate novel autoantibody specificities. The recent recognition of these novel specificities in RA provides a unique opportunity to understand human B-cell development in vivo. In this paper, we will review the three of the main classes of PTMs already associated with RA: citrullination, carbamylation, and oxidation. With the advancement of research methodologies it should be expected that other autoantibodies against PTM proteins could be discovered in patients with autoimmune diseases. Many of such autoantibodies may provide significant biomarker potential