Großherzogin Luise von Baden - eine politische Biographie(1838-1923)

Es folgt ein Werkstattbericht meines Promotionsprojektes "Großherzogin Luise von Baden - eine politische Biographie (1838 - 1923). Dargestellt werden die dynastischen Bezüge der Großherzogin im Kontext europäischer Herrschaftsverhältnisse des 19. Jahrhunderts, ihre Tätigkeitsbereiche Badischer Frauenverein und Rotes Kreuz, sowie die Quellenlage des Geheimen Kabinetts der Großherzogin Luise von Baden

Rezension: Birgit Bublies-Godau (Hg.) "Dass Frauen bessere Demokraten, geborene Demokraten seyen ..." Henriette Obermüller-Venedey. Tagebücher und Lebenserinnerungen 1817 - 11871. (Schriftenreihe des Stadtarchivs Karlsruhe, Bd.7) Karlsruhe 1999, Badenia Verlag, 280 Seiten, 22 Abbildungen.

Birgit Bublies-Godau präsentiert erstmals die autobiographischen Schriften der badischen Revolutionärin und frühen bürgerlichen „Politikerin“, sie sind vollständig transkribiert und profund kommentiert. Eine sensibel recherchierte biographische Einführung zu Henriette Obermüller-Venedey (1817 – 1893) eröffnet die vorliegende Edition. Sie stellt den wechselvollen Lebensweg der Autorin hin zur aktiven Demokratin, Republikanerin und Revolutionärin, Gattin, Mutter, Hausfrau sowie erfolgreichen Weinhändlerin, Pensionswirtin und Unternehmerin vor: In chronologischer Reihe ediert Bublies-Godau die Ego-Dokumente von Henriette Obermüller-Venedey: Ihre beiden Tagebücher und ihre Lebenserinnerungen, welche die Autorin im Zeitraum von Mai 1856 und Februar 1871 niedergeschrieben hatte. Entsprechend ihrer chronologischen Entstehung folgen „Notizen unserer Erlebnisse gesammelt im Jahr 1856 in Heidelberg“ und „Erlebnisse in Oberweiler. März 1869“, sowie die „Beschreibung meines Lebens“ nacheinander. Eine umfassende Literaturliste, Register und Kommentar runden die Edition ab und machen sie als Nachlagewerk nutzbar

Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kinetics

<p>Abstract</p> <p>Background</p> <p>There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually conducted in batch mode without active pH-control, but with high buffer concentrations to prevent excessive pH-drifts. These differences make it difficult to compare results from screening experiments and laboratory and technical scale cultivations and, thus, complicate rational process development. In particular, the pH-value plays an important role in fermentation processes due to the narrow physiological or optimal pH-range of microorganisms. To reduce the differences between the scales and to establish a pH-control in shake flasks, a newly developed easy to use polymer-based controlled-release system is presented in this paper. This system consists of bio-compatible silicone discs embedding the alkaline reagent Na₂CO₃. Since the sodium carbonate is gradually released from the discs in pre-determined kinetics, it will ultimately compensate the decrease in pH caused by the biological activity of microorganisms.</p> <p>Results</p> <p>The controlled-release discs presented here were successfully used to cultivate <it>E. coli </it>K12 and <it>E. coli </it>BL21 pRSET eYFP-IL6 in mineral media with glucose and glycerol as carbon (C) sources, respectively. With glucose as the C-source it was possible to reduce the required buffer concentration in shake flask cultures by 50%. Moreover, with glycerol as the C-source, no buffer was needed at all.</p> <p>Conclusions</p> <p>These novel polymer-based controlled-release discs allowed buffer concentrations in shake flask media to be substantially reduced or omitted, while the pH remains in the physiological range of the microorganisms during the whole cultivation time. Therefore, the controlled-release discs allow a better control of the pH, than merely using high buffer concentrations. The conditions applied here, i.e. with significantly reduced buffer concentrations, enhance the comparability of the culture conditions used in screening experiments and large-scale fermentation processes.</p

Magnetron deposition of TCO films using ion beam

Thin films of tin oxide (TO) were deposited on the glass substrates at room temperature using reactive magnetron sputtering at various oxygen partial pressures. After the deposition the films were irradiated with argon ions beam. The change of the optical and electrical properties of the films depending on the irradiation time was studied. Films optical properties in the range of 300-1100 nm were investigated by photometry as well as their structural properties were studied using X-ray diffraction. Diffractometric research showed that the films, deposited on a substrate, have a crystal structure, and after argon ions irradiation they become quasi-crystalline (amorphous). It was found that the transmission increases proportionally with the irradiation time, but the surface resistance -disproportionally

Process development in Hansenula polymorpha and Arxula adeninivorans, a re-assessment

A range of industrial H. polymorpha-based processes exist, most of them for the production of pharmaceuticals. The established industrial processes lean on the use of promoters derived from MOX and FMD, genes of the methanol metabolism pathway. In Hansenula polymorpha these promoters are de-repressed upon depletion of a range of carbon sources like glucose and glycerol instead of being induced by methanol as reported for other methylotrophs. Due to these characteristics screening and fermentation modes have been defined for strains harbouring such expression control elements that lean on a limited supplementation of glycerol or glucose to a culture medium. For fermentation of H. polymorpha a synthetic minimal medium (SYN6) has been developed. No industrial processes have been developed so far based on Arxula adeninivorans and only a limited range of strong promoter elements exists, suitable for heterologous gene expression. SYN6 originally designed for H. polymorpha provided a suitable basis for the initial definition of fermentation conditions for this dimorphic yeast. Characteristics like osmo- and thermotolerance can be addressed for the definition of culture conditions

Search for the Higgs boson in events with missing transverse energy and b quark jets produced in proton-antiproton collisions at s**(1/2)=1.96 TeV

We search for the standard model Higgs boson produced in association with an electroweak vector boson in events with no identified charged leptons, large imbalance in transverse momentum, and two jets where at least one contains a secondary vertex consistent with the decay of b hadrons. We use ~1 fb-1 integrated luminosity of proton-antiproton collisions at s**(1/2)=1.96 TeV recorded by the CDF II experiment at the Tevatron. We find 268 (16) single (double) b-tagged candidate events, where 248 +/- 43 (14.4 +/- 2.7) are expected from standard model background processes. We place 95% confidence level upper limits on the Higgs boson production cross section for several Higgs boson masses ranging from 110 GeV/c2 to 140 GeV/c2. For a mass of 115 GeV/c2 the observed (expected) limit is 20.4 (14.2) times the standard model prediction.Comment: 8 pages, 2 figures, submitted to Phys. Rev. Let

Measurement of the Dipion Mass Spectrum in X(3872) -> J/Psi Pi+ Pi- Decays

We measure the dipion mass spectrum in X(3872)--> J/Psi Pi+ Pi- decays using 360 pb-1 of pbar-p collisions at 1.96 TeV collected with the CDF II detector. The spectrum is fit with predictions for odd C-parity (3S1, 1P1, and 3DJ) charmonia decaying to J/Psi Pi+ Pi-, as well as even C-parity states in which the pions are from Rho0 decay. The latter case also encompasses exotic interpretations, such as a D0-D*0Bar molecule. Only the 3S1 and J/Psi Rho hypotheses are compatible with our data. Since 3S1 is untenable on other grounds, decay via J/Psi Rho is favored, which implies C=+1 for the X(3872). Models for different J/Psi-Rho angular momenta L are considered. Flexibility in the models, especially the introduction of Rho-Omega interference, enable good descriptions of our data for both L=0 and 1.Comment: 7 pages, 4 figures -- Submitted to Phys. Rev. Let