Elucidating the role of a novel DNA-binding protein identified in the diatom Thalassiosira pseudonana for coping with nutrient and temperature stress

Diatoms are associated with nutrient rich waters with high biomass that are commonly found at high latitudes, in coastal waters, in upwelling zones or during seasonal blooms.They display an opportunistic growth described as a ’bloom and bust’ life cyce and a remarkable diversity. These caracteristics are considered to be responsible for their success in aquatic environments and the reason why they contribute about 20% of global primary production and allow them to quickly dominate phytoplankton communities when environmental conditions become favourable. The processes that enable this impressive plasticity of diatoms in response to environmental variability are mostly unknown. A novel conserved DNA binding protein (BIG1) was recently identified and found to differentially regulate genes involved in progression through the cell cycle in centric diatoms as well as about 30% of genes found in natural centric diatom blooms. In our study we show that the BIG1 protein is linked to the regulation of initiation of fast growth upon nutrient addition. Overexpression of BIG1 in the model centric diatom Thalassiosira pseudonana enabled fast recovery and growth after a period of nitrogen stravation and exposure to low and high temperature as well as fostered growth under suboptimal temperatures. We present evidence that BIG1 has an impact on photosynthesis and suggest its involvement in directing the photosynthetic electron flow around Photosystem I. Our results may provide insights into regulatory processes that govern diatoms’ oportunistic growth

Mapping Nearshore Bathymetry with Spaceborne Data Fusion and State Space Modeling

Despite numerous techniques for measuring and estimating water depth, bathymetry in the nearshore zone is notoriously difficult to map. Dangerous sea states, noisy environmental conditions, and expensive survey operations, particularly in remote areas, contribute to the difficulties of obtaining data along the coast. Global datasets, derived mainly from satellite altimetry methods, do exist, but they have significant limitations nearshore. Numerous high-resolution datasets, conventionally acquired with acoustic and lidar techniques, also exist, but they cover only a small percentage of the world's coasts. Spaceborne data fusion employing multispectral satellite derived bathymetry (SDB) offers the potential to significantly reduce the global lack of nearshore bathymetry, coined the "white ribbon" by the hydrographic community, referring to the alongshore data gap on many nautical charts. A broad term, multispectral SDB spans a diverse spectrum of methods that have been used extensively in specific case studies, but the application of multispectral SDB on a global or regional scale is significantly limited by the availability of in situ reference depths needed to tune derived values. Additionally, many existing approaches only use a single multispectral image, which can result in significant errors or missing data if the image contains environmental or sensor noise, such as clouds, sediment plumes, or detector-edge artifacts. This dissertation presents two spaceborne empirical multispectral SDB methods to address shortcomings of existing SDB approaches and reduce the global shortage of nearshore bathymetry – (1) active/passive spaceborne data fusion combining MABEL/ICESat-2 and multispectral data and (2) state space modeling of Sentinel-2 and Landsat 8 multispectral data to generate gap-free models of relative SDB (rSDB) with corresponding uncertainty estimates. The recently launched ICESat-2 mission offers an opportunity for a completely spaceborne active-passive data fusion approach to nearshore bathymetry by potentially providing a global source of nearshore reference depths to tune empirical multispectral SDB algorithms. The main objectives of the ICESat-2 mission are to measure ice-sheet elevations, sea-ice thickness, and global biomass, but ICESat-2’s 532-nm wavelength photon-counting Advanced Topographic Laser Altimeter System (ATLAS) was first posited, then demonstrated capable of detecting bathymetry in certain nearshore environments. Presented in two studies conducted prior to ICESat-2’s launch, the active-passive approach is demonstrated with data from MABEL, NASA’s high-altitude ATLAS simulator system. The first study assessed the ability to derive bathymetry from MABEL and then evaluated the accuracy and reliability of MABEL bathymetry using data acquired in Keweenaw Bay, Lake Superior. The study also developed and verified a baseline model to predict numbers of bottom returns as a function of water depth. The second study completed the demonstration of the spaceborne active/passive data fusion method by synergistically fusing MABEL-derived bathymetry and Landsat 8 multispectral Operational Land Imager (OLI) imagery over the entire Keweenaw Bay study site using the Stumpf band-ratio algorithm. The study also assessed the spatiotemporal viability of the data fusion approach by characterizing the variability of global coastal water clarity as interpreted from Visible Infrared Imaging Radiometer Suite (VIIRS) Kd(490) data. The calculated SDB agreed with a high-resolution topobathymetric lidar dataset to within an RMSE of 0.7 m, and the spatiotemporal viability analysis indicated that the spaceborne active-passive data fusion approach may be viable over many regions of the globe throughout the course of a year. State space modeling of empirical multitemporal SDB overcomes limitations of single-image SDB by leveraging the bathymetric signal in multispectral time series to create gap-free models of relative SDB (rSDB) for an arbitrary date, enabling SDB for dates with noisy or no data. State space models (SSMs) are well established in many applications but are absent in empirical SDB literature. Consisting of a state equation, which relates consecutive state vectors, and an observation equation, which relates observations to the state vector, SSMs are typically solved using Kalman filtering techniques, which provide estimates of uncertainties along with state estimates. SSMs also provide a mechanism for data fusion by allowing an observation equation for multiple observed time series. The third study demonstrates a state space approach to empirical multispectral SDB by applying local level SSMs to Landsat 8 OLI and Sentinel-2 MSI rSDB time series, both separately and fused. A representative single-sensor SSM (Landsat 8) was transformed to SDB that agreed with a high-resolution topobathymetric lidar dataset to within an RMSE of 0.29 m, which indicates the promising performance of the state space framework. Internally consistent fused-sensor SSMs verified that state space modeling also offers a data-fusion method capable of incorporating time series from a diverse suite of multispectral sensors

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a Gram-negative bacterium that represents the main cause of porcine pleuropneumonia in pigs, causing significant economic losses to the livestock industry worldwide. A. pleuropneumoniae, as the majority of Gram-negative bacteria, excrete vesicles from its outer membrane (OM), accordingly defined as outer membrane vesicles (OMVs). Thanks to their antigenic similarity to the OM, OMVs have emerged as a promising tool in vaccinology. In this study we describe the in vivo testing of several vaccine prototypes for the prevention of infection by all known A. pleuropneumoniae serotypes. Previously identified vaccine candidates, the recombinant proteins ApfA and VacJ, administered individually or in various combinations with the OMVs, were employed as vaccination strategies. Our data show that the addition of the OMVs in the vaccine formulations significantly increased the specific IgG titer against both ApfA and VacJ in the immunized animals, confirming the previously postulated potential of the OMVs as adjuvant. Unfortunately, the antibody response raised did not translate into an effective protection against A. pleuropneumoniae infection, as none of the immunized groups following challenge showed a significantly lower degree of lesions than the controls. Interestingly, quite the opposite was true, as the animals with the highest IgG titers were also the ones bearing the most extensive lesions in their lungs. These results shed new light on A. pleuropneumoniae pathogenicity, suggesting that antibody-mediated cytotoxicity from the host immune response may play a central role in the development of the lesions typically associated with A. pleuropneumoniae infections

Современные взгляды на ультразвуковую диагностику внутреннего эндометриоза

Представлен обзор данных о применении различных методик в диагностике эндометриоза. Показаны преимущества и высокая информативность трансвагинальной эхографии. Описаны эхографические критерии различных стадий эндометриоза. Отмечена связь эндометриоза с разными патологиями щитовидной и молочной желез.This paper reviews the data on the use of different techniques of endometriosis diagnosis. The advantages and high informativity of transvaginal ultrasonography are demonstrated. Ultrasonography criteria of different stages of endometriosis are described. Association of endometriosis and thyroid and breast pathology is demonstrated

Identification of autoantibodies to the I protein of the heterogeneous nuclear ribonucleoprotein complex in patients with systemic sclerosis

Objective. To assess the presence of autoantibodies to the I protein (polypyrimidine-tract binding protein) of the heterogeneous nuclear RNPs (hnRNP) in different connective tissue diseases. Antibodies to other hnRNP proteins (A1, A2, and B) have been previously found in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and mixed connective tissue disease (MCTD). Methods. Sera from 101 patients with various connective tissue diseases and 25 normal controls were investigated by enzyme-linked immunosorbent assay and immunoblotting, for their reactivity to highly purified recombinant hnRNP I. Moreover, reactivity to cellular hnRNP I protein was investigated by immunoblotting using a partially purified preparation of hnRNP proteins (including A1, A2, B, and I), and by indirect immunofluorescence. For the analysis of the fluorescence pattern, affinity-purified antibodies to hnRNP I, obtained from a selected patient, were tested on HEp-2 cells. Results. By immunoblotting, antibodies reacting to recombinant hnRNP I were found in 22 of 40 patients with systemic sclerosis (SSc), 3 of 32 with RA, 0 of 23 with SLE, and 0 of 6 with MCTD. Antibodies to recombinant hnRNP I were more frequently found in patients with pre-SSc or limited SSc (15 of 24) than in those with intermediate or diffuse SSc (7 of 16). In indirect immunofluorescence studies, affinity-purified anti-hnRNP I autoantibodies gave a diffuse nucleoplasmic staining. Using an hnRNP preparation from nuclear extracts, anti-hnRNP I reactivity was detectable in SSc sera, while it was not detectable in RA, SLE, and MCTD sera reacting with hnRNP A/B proteins. Conclusion. Human autoimmune sera show distinct patterns of anti-hnRNP reactivity, i.e., anti-A/B in SLE and RA sera, and anti-I in SSc sera. This suggests that A/B proteins and the I protein may be involved in different dynamic hnRNP complexes that elicit different autoimmune responses. From a clinical perspective, anti-hnRNP I antibodies are frequently associated with pre-SSc features, suggesting an early appearance of these antibodies during the course of the disease