Presence of structural homologs of ubiquitin in haloalkaliphilic Archaea

Ubiquitin, a protein widely conserved in eukaryotes, is involved in many cellular processes, including proteolysis. While sequences encoding ubiquitin-like proteins have not been identified in prokaryotic genomes sequenced so far, they have revealed the presence of structural and functional homologs of ubiquitin in Bacteria and Archaea. This work describes the amplification and proteomic analysis of a 400-bp DNA fragment from the haloalkaliphilic archaeon Natrialba magadii. The encoded polypeptide, P400, displayed structural homology to ubiquitin-like proteins such as those of the ThiS family and Urm1. Expression of the P400 DNA sequence in Escherichia coli cells yielded a recombinant polypeptide that reacted with anti-ubiquitin antibodies. In addition, a putative open reading frame encoding P400 was identified in the recently sequenced genome of N. magadii. Together, these results evidence the presence in Archaea of structural homologs of ubiquitin- related proteins. [Int Microbiol 2009; 12(3):167-173

Vida e morte: a educação com arte. Um projeto do PIBID de Psicologia no Ensino Médio

O artigo em questão é resultado das atividades desenvolvidas na disciplina eletiva de Filosofia em uma escola estadual de Goiás intitulada Vida e Morte: o que a arte tem a nos dizer sobre isso? Essa disciplina fez parte das atividades desenvolvidas pelo subprojeto de Psicologia do Programa Institucional de Bolsas de Iniciação à Docência (PIBID) da Universidade Federal de Goiás (UFG). Esse subprojeto tem como objetivo discutir e refletir temas da Psicologia com os alunos do Ensino Médio, a fim de contribuir com uma formação mais reflexiva e crítica dos mesmos. Essa disciplina visava fomentar discussões acerca de temas angulares da vida humana com apoio nas bases da Psicanálise e da expressão artística. Buscou-se demonstrar como a utilização da arte enquanto metodologia e recurso didático foram de fundamental importância para o ensino de Psicologia para adolescentes. Além de destacar a importância de ser reservado um espaço na escola para se falar sobre vida, morte e afetos e sobre como a Psicologia se insere nesse campo

Vida e morte: a educação com arte. Um projeto do PIBID de Psicologia no Ensino Médio

O artigo em questão é resultado das atividades desenvolvidas na disciplina eletiva de Filosofia em uma escola estadual de Goiás intitulada Vida e Morte: o que a arte tem a nos dizer sobre isso? Essa disciplina fez parte das atividades desenvolvidas pelo subprojeto de Psicologia do Programa Institucional de Bolsas de Iniciação à Docência (PIBID) da Universidade Federal de Goiás (UFG). Esse subprojeto tem como objetivo discutir e refletir temas da Psicologia com os alunos do Ensino Médio, a fim de contribuir com uma formação mais reflexiva e crítica dos mesmos. Essa disciplina visava fomentar discussões acerca de temas angulares da vida humana com apoio nas bases da Psicanálise e da expressão artística. Buscou-se demonstrar como a utilização da arte enquanto metodologia e recurso didático foram de fundamental importância para o ensino de Psicologia para adolescentes. Além de destacar a importância de ser reservado um espaço na escola para se falar sobre vida, morte e afetos e sobre como a Psicologia se insere nesse campo

Relations between research and clinical care in co-management studies with mental health care users

O artigo parte da experiência de pesquisar com usuários de saúde mental (e não sobre eles, ou para eles), narrando cenas de um estudo no qual se colocaram em jogo modos distintos de articulação entre pesquisa e clínica, problematizando suas fronteiras e as questões éticas implicadas. Situa o campo da clínica e da pesquisa de que se trata com o aporte de autores que, desde a análise institucional, propõem a ideia de pesquisaintervenção; e, no contexto da saúde coletiva, resgatam o conceito de clínica ampliada. Fundamenta- se a articulação entre esses dois termos – pesquisa- intervenção e clínica ampliada – desde a noção de subjetividade que opera no âmbito da saúde coletiva e que culmina na ideia de autonomia. Finalmente, propõe-se a cogestão como estratégia a partir da qual os diferentes atores implicados na condução da pesquisa e no exercício da clínica constroem coletivamente uma direção de trabalho, ao mesmo tempo terapêutica e ética.This paper is derived from the experience of conducting research with mental health users (not about them, nor for them), analyzing aspects of a study in which different ways of structuring the relationship between clinical practice and research were put into play, thereby questioning the boundaries and ethical issues involved. The clinical practice and research fields that are dealt with are studied with the input of authors who, on the basis of institutional analysis, propose the idea of interventional research, and in the context of public health, revert to the concept of broadened clinical care. The relationship between these two terms – interventional research and broadened clinical care – is based on the notion of subjectivity that operates within the scope of public health and which culminates in the concept of autonomy. Lastly, co-management is proposed as a strategy based on which the different actors involved in conducting research and exercising clinical care can collectively build working principles that are both therapeutic and ethical

Autonomia e cogestão na prática em saúde mental: o dispositivo da gestão autônoma da medicação (GAM)

O artigo aborda a articulação entre autonomia e cogestão nas práticas em saúde mental no Brasil, baseado em estudo multicêntrico. Tal estudo objetivou a elaboração do Guia Brasileiro da Gestão Autônoma da Medicação (Guia GAM-BR), com base na tradução e adaptação do Guia GAM desenvolvido no Quebec – instrumento dirigido a pessoas com transtornos mentais graves. Uma primeira versão do Guia GAM traduzida e adaptada ao contexto brasileiro foi utilizada em Grupos de Intervenção (GI) com usuários de serviços de saúde mental nos campos da pesquisa. A construção da versão final do Guia GAM brasileiro incluiu as modificações propostas pelos GI em cada campo, debatidas em reuniões multicêntricas com a participação de pesquisadores, trabalhadores e usuários integrantes dos GI. No curso da pesquisa, a estratégia GAM assumiu o desafio de propor-se como prática cogestiva, compatibilizando exercício da autonomia, direito e protagonismo dos usuários com o funcionamento e cultura organizacional das instituições de saúde mental

Autonomy and co-management in mental health practice : the autonomous medication management device (AMM)

O artigo aborda a articulação entre autonomia e cogestão nas práticas em saúde mental no Brasil, baseado em estudo multicêntrico. Tal estudo objetivou a elaboração do Guia Brasileiro da Gestão Autônoma da Medicação (Guia GAM-BR), com base na tradução e adaptação do Guia GAM desenvolvido no Quebec – instrumento dirigido a pessoas com transtornos mentais graves. Uma primeira versão do Guia GAM traduzida e adaptada ao contexto brasileiro foi utilizada em Grupos de Intervenção (GI) com usuários de serviços de saúde mental nos campos da pesquisa. A construção da versão final do Guia GAM brasileiro incluiu as modificações propostas pelos GI em cada campo, debatidas em reuniões multicêntricas com a participação de pesquisadores, trabalhadores e usuários integrantes dos GI. No curso da pesquisa, a estratégia GAM assumiu o desafio de propor-se como prática cogestiva, compatibilizando exercício da autonomia, direito e protagonismo dos usuários com o funcionamento e cultura organizacional das instituições de saúde mental.The present paper discusses the relation of autonomy and co-management on Brazil´s mental health practices following a previous multicenter approach. That approach aimed at translating, adapting, and testing in Brazil the Autonomous Medication Management Guide (AMMG), a Canadian tool directed for people with severe mental disorders. A first version of the AMMG, translated and adapted to the Brazilian context, was applied through Intervention Groups (IGs) of mental health users at each research center. Resulting on a final version of the Brazilian AMMG including modifications discussed in multicenter meetings where researchers, workers, and users of the IGs attended. The AMM strategy has therefore taken the challenge to propose itself as a co-management mental health practice, combining exercise of autonomy, rights and role of the users with the operation and organizational culture of mental health institutions

Retrospective study of clinical cases in ruminants at the UFRGS veterinary teaching hospital

Background: The pillars of animal production are sanity, genetics and nutrition. Sanitary control of the herd is important to reduce production costs and maintain health. The Veterinary Clinics Hospital (HCV) of the Federal University of Rio Grande do Sul (UFRGS), located in Porto Alegre - RS, is the place of greatest casuistry of the state, with 20.000 annual attendances, between small and large animals. In view of this scenario, the present study aimed to determine the frequency and characterize the ruminants attended at HCV, in order to help in the future, in the construction of control and prevention strategies of diseases found. Materials, Methods & Results: The documents of ruminant care between January 2007 and May 2018 were searched in the archives of the Hospital of Veterinary Clinics of UFRGS. Data on species, race, sex, age and diagnosis were collected. Diagnoses were classified as conclusive and inconclusive and the cases with conclusive diagnosis were classified according to etiology: infectious and parasitic diseases, metabolic and nutritional diseases, reproductive and obstetric diseases, toxic diseases, traumatic diseases. The prevalence of diseases and characteristics of ruminants attended (species, sex, category) was calculated. During the study period, between January 2007 and May 2018, 341 ruminants were attended, with emphasis on sheep (42%), goats (39%) and cattle (18%). In addition, a camel with foreign body obstruction, a sambar deer with fracture of the first thoracic vertebra and a buffalo with ruminal impaction were attended. The care profile was mapped, with predominance of females (57%) and adults (59%). Most of the animals did not present a defined breed, but among the breeds stands out Texel, of cutting aptitude, in sheeps and Saanen and Holstein, of dairy aptitude, in goats and cattle, respectively. In the retrospective study, the diseases with infectious and parasitic etiology presented the highest prevalence with 27.5% of the attendances, followed by reproductive (17.5%), traumatic (13.5%), metabolic (10%), others (10%) and toxic (2.5%). Sixty-six animals had inconclusive diagnosis (19.5%). Discussion: The highest prevalences were infectious and parasitic diseases, with emphasis on myiasis and verminosis. So, it is perceived that management corrections are sufficient to reduce the number of occurrences of diseases and prophylactic measures such as vaccination protocol, vermifugation and adequate nutritional management are allied in this walk. Among the reproductive diseases, dystocia (42.85%), which is one of the main causes of mortality in the peripartum period, has been highlighted. Dog attack was the major cause of traumas in ruminants and urolithiasis was highlighted in metabolic diseases. In toxic diseases, copper intoxication was the most important. Sheep are extremely sensitive to this intoxication, as they tend to accumulate copper in the organism. The retrospective study made it possible to visualize the panorama of HCV UFRGS visits to ruminants in the last years, mapping the profile and determining the casuistry of the diseases. Studies of hospital veterinary casuistry are rare, mainly involving ruminants. At the end, it is concluded that studies referring to casuistry are important to know the predominant diseases in a specific area and its risk factors considering differential diagnosis and future prevention programs

Retrospective Study of Clinical Cases in Ruminants at the UFRGS Veterinary Teaching Hospital

Background: The pillars of animal production are sanity, genetics and nutrition. Sanitary control of the herd is important to reduce production costs and maintain health. The Veterinary Clinics Hospital (HCV) of the Federal University of Rio Grande do Sul (UFRGS), located in Porto Alegre - RS, is the place of greatest casuistry of the state, with 20.000 annual attendances, between small and large animals. In view of this scenario, the present study aimed to determine the frequency and characterize the ruminants attended at HCV, in order to help in the future, in the construction of control and prevention strategies of diseases found. Materials, Methods & Results: The documents of ruminant care between January 2007 and May 2018 were searched in the archives of the Hospital of Veterinary Clinics of UFRGS. Data on species, race, sex, age and diagnosis were collected. Diagnoses were classified as conclusive and inconclusive and the cases with conclusive diagnosis were classified according to etiology: infectious and parasitic diseases, metabolic and nutritional diseases, reproductive and obstetric diseases, toxic diseases, traumatic diseases. The prevalence of diseases and characteristics of ruminants attended (species, sex, category) was calculated. During the study period, between January 2007 and May 2018, 341 ruminants were attended, with emphasis on sheep (42%), goats (39%) and cattle (18%). In addition, a camel with foreign body obstruction, a sambar deer with fracture of the first thoracic vertebra and a buffalo with ruminal impaction were attended. The care profile was mapped, with predominance of females (57%) and adults (59%). Most of the animals did not present a defined breed, but among the breeds stands out Texel, of cutting aptitude, in sheeps and Saanen and Holstein, of dairy aptitude, in goats and cattle, respectively. In the retrospective study, the diseases with infectious and parasitic etiology presented the highest prevalence with 27.5% of the attendances, followed by reproductive (17.5%), traumatic (13.5%), metabolic (10%), others (10%) and toxic (2.5%). Sixty-six animals had inconclusive diagnosis (19.5%). Discussion: The highest prevalences were infectious and parasitic diseases, with emphasis on myiasis and verminosis. So, it is perceived that management corrections are sufficient to reduce the number of occurrences of diseases and prophylactic measures such as vaccination protocol, vermifugation and adequate nutritional management are allied in this walk. Among the reproductive diseases, dystocia (42.85%), which is one of the main causes of mortality in the peripartum period, has been highlighted. Dog attack was the major cause of traumas in ruminants and urolithiasis was highlighted in metabolic diseases. In toxic diseases, copper intoxication was the most important. Sheep are extremely sensitive to this intoxication, as they tend to accumulate copper in the organism. The retrospective study made it possible to visualize the panorama of HCV UFRGS visits to ruminants in the last years, mapping the profile and determining the casuistry of the diseases. Studies of hospital veterinary casuistry are rare, mainly involving ruminants. At the end, it is concluded that studies referring to casuistry are important to know the predominant diseases in a specific area and its risk factors considering differential diagnosis and future prevention programs