Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria

A nuclear encoded mitochondrial heat-shock protein hsp60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. hsp60 is a member of the 'chaperonin' class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplast

Accounting Induced Distortions in Public Enterprise Pricing

Certain government enterprises not subject to competition (e.g., municipal utilities, irrigation agencies) commonly aim to set prices at average cost. In periods of inflation, unadjusted accounting data understate the economic costs of fixed assets and, thus, generate inefficiently low prices and high consumption rates for outputs of these enterprises. Since dividends and taxes are not involved, the primary function of accounting is cost determination rather than profit measurement. We measure the nature and extent of undercosting and underpricing for a group of government owned water utilities in the arid western United States. Economic costs appear to be two to three times their accounting counterparts for the cases studied.

Trace gas measurements from whole air samples collected over the Antarctic continent

Whole air samples collected aboard the NASA DC-8 and ER-2 aircraft as part of the Airborne Antarctic Ozone Experiment (AAOE) were analyzed in a field laboratory set up at Punta Arenas, Chile, in August and September, 1987. Mixing ratios obtained from gas chromatographic analyses of these samples are presented for N2O, CFCl3, CFCl2, C2F3Cl3, CH3CCl3, CH4, and CO. Variations in the mixing ratios of these gases along the individual flight paths of the aircraft are used as tracers to indicate the history of air masses over and near the Antarctic continent

hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases

This work was supported by a National Health and Medical Research Council project grant [1066550], an Australian Research Council project grant [DP 120103099] and by a Queensland Health Senior Clinical Research Fellowship awarded to K.J.O. This work was also supported by the Wellcome Trust [094476/Z/10/Z], which funded the purchase of the TripleTOF 5600 mass spectrometer at the BSRC Mass Spectrometry and Proteomics Facility, University of St Andrews. NWA was supported by a scholarship awarded by Cancer Council Queensland. E.B. is supported by an Advance Queensland Research Fellowship.The maintenance of genomic stability is essential for cellular viability and the prevention of diseases such as cancer. Human single-stranded DNA-binding protein 1 (hSSB1) is a protein with roles in the stabilisation and restart of stalled DNA replication forks, as well as in the repair of oxidative DNA lesions and double-strand DNA breaks. In the latter process, phosphorylation of threonine 117 by the ATM kinase is required for hSSB1 stability and efficient DNA repair. The regulation of hSSB1 in other DNA repair pathways has however remained unclear. Here we report that hSSB1 is also directly phosphorylated by DNA-PK at serine residue 134. While this modification is largely suppressed in undamaged cells by PPP-family protein phosphatases, S134 phosphorylation is enhanced following the disruption of replication forks and promotes cellular survival. Together, these data thereby represent a novel mechanism for hSSB1 regulation following the inhibition of replication.Publisher PDFPeer reviewe

Historical Perspective

In Re: Amundsen P. Cerebral angiography via the femoral artery with particular reference to cerebrovascular disease

Viral CpG Deficiency Provides No Evidence That Dogs Were Intermediate Hosts for SARS-CoV-2.

Due to the scope and impact of the COVID-19 pandemic there exists a strong desire to understand where the SARS-CoV-2 virus came from and how it jumped species boundaries to humans. Molecular evolutionary analyses can trace viral origins by establishing relatedness and divergence times of viruses and identifying past selective pressures. However, we must uphold rigorous standards of inference and interpretation on this topic because of the ramifications of being wrong. Here, we dispute the conclusions of Xia (2020. Extreme genomic CpG deficiency in SARS-CoV-2 and evasion of host antiviral defense. Mol Biol Evol. doi:10.1093/molbev/masa095) that dogs are a likely intermediate host of a SARS-CoV-2 ancestor. We highlight major flaws in Xia's inference process and his analysis of CpG deficiencies, and conclude that there is no direct evidence for the role of dogs as intermediate hosts. Bats and pangolins currently have the greatest support as ancestral hosts of SARS-CoV-2, with the strong caveat that sampling of wildlife species for coronaviruses has been limited

Evolution of the Chandra CCD Spectra of SNR 1987A: Probing the Reflected-Shock Picture

We continue to explore the validity of the reflected shock structure (RSS) picture in SNR 1987A that was proposed in our previous analyses of the X-ray emission from this object. We used an improved version of our RSS model in a global analysis of 14 CCD spectra from the monitoring program with Chandra. In the framework of the RSS picture, we are able to match both the expansion velocity curve deduced from the analysis of the X-ray images and light curve. Using a simplified analysis, we also show that the X-rays and the non-thermal radio emission may originate from the same shock structure (the blast wave). We believe that using the RSS model in the analysis of grating data from the Chandra monitoring program of SNR 1987A that cover a long enough time interval, will allow us to build a more realistic physical picture and model of SNR 1987A.Comment: 14 pages, 1 Table, 8 figures, accepted for publication in MNRA

Captive reptile mortality rates in the home and implications for the wildlife trade

The trade in wildlife and keeping of exotic pets is subject to varying levels of national and international regulation and is a topic often attracting controversy. Reptiles are popular exotic pets and comprise a substantial component of the live animal trade. High mortality of traded animals raises welfare concerns, and also has implications for conservation if collection from the wild is required to meet demand. Mortality of reptiles can occur at any stage of the trade chain from collector to consumer. However, there is limited information on mortality rates of reptiles across trade chains, particularly amongst final consumers in the home. We investigated mortality rates of reptiles amongst consumers using a specialised technique for asking sensitive questions, additive Randomised Response Technique (aRRT), as well as direct questioning (DQ). Overall, 3.6% of snakes, chelonians and lizards died within one year of acquisition. Boas and pythons had the lowest reported mortality rates of 1.9% and chameleons had the highest at 28.2%. More than 97% of snakes, 87% of lizards and 69% of chelonians acquired by respondents over five years were reported to be captive bred and results suggest that mortality rates may be lowest for captive bred individuals. Estimates of mortality from aRRT and DQ did not differ significantly which is in line with our findings that respondents did not find questions about reptile mortality to be sensitive. This research suggests that captive reptile mortality in the home is rather low, and identifies those taxa where further effort could be made to reduce mortality rate

Isospin breaking corrections to nucleon form factors in the constituent quark model

We examine isospin breaking in the nucleon wave functions due to the $u - d$ quark mass difference and the Coulomb interaction among the quarks, and their consequences on the nucleon electroweak form factors in a nonrelativistic constituent quark model. The mechanically induced isospin breaking in the nucleon wave functions and electroweak form factors are exactly evaluated in this model. We calculate the electromagnetically induced isospin admixtures by using first-order perturbation theory, including the lowest-lying resonance with nucleon quantum numbers but isospin 3/2. We find a small ($\leq 1\%$), but finite correction to the anomalous magnetic moments of the nucleon stemming almost entirely from the quark mass difference, while the static nucleon axial coupling remains uncorrected. Corrections of the same order of magnitude appear in charge, magnetic, and axial radii of the nucleon. The correction to the charge radius in this model is primarily isoscalar, and may be of some significance for the extraction of the strangeness radius from e.g. elastic forward angle parity violating electron-proton asymmetries, or elastic ${}^4He({\vec e},e')$ experiments.Comment: 15 pp(22 as preprint), revtex, 3 uuencoded figs at end of this fil