Experimental Investigation of Particulate Deposition on a Simulated Film-Cooled Turbine Vane Pressure Surface in a High Pressure Combustion Facility

Use of coal syngas for Integrated Gasification Combined Cycle (IGCC) industrial gas turbines introduces contaminants into the flow that can deposit onto the components of the first stage of the turbine. These deposit structures may create alterations in the cooling scheme and can erode or react with thermal barrier coatings (TBC). A study was performed to examine the evolution and contributing factors to the growth of deposit structures in a simulated gas turbine environment. Tests were performed in the high pressure/temperature aerothermal facility at the Department of Energy\u27s (DoE) National Energy Technology Laboratory (NETL) in Morgantown, WV. The facility was operated at a pressure of 45 psig and temperatures ranging from 1950°F to 2350°F. Two test article geometries with four film cooling holes non-dimensionally matched to a large-scale industrial gas turbine were created to simulate the pressure side of a first stage vane. A high pressure seeding system was developed to inject coal fly-ash to simulate the build-up of particulate matter experienced by industrial gas turbines into the high pressure facility to perform accelerated deposition of the fly-ash onto the test articles. A method was developed to process the fly-ash to match the theoretical size distribution and particle flow dynamics representative of an industrial gas turbine scaled. Analyses were performed to determine whether the particles reached thermal equilibrium before impacting the test article and to estimate the penetration depth of the particles from the injection tube into the mainstream flow of the facility cross flow. Five independent variable effects were studied; impaction angle, freestream temperature, blowing ratio, surface (TBC or no TBC), and increases in simulated operating hours. In studying the effects of surface impaction angle, deposition increased as the face angle of the test article increased from 10° to 20°. Variation of the freestream temperature showed that the deposition was dependent on a theoretical sticking freestream temperature of 2315oF. Deposition resisted forming at temperatures below the theoretical sticking temperature. In studying the effects of blowing ratio, deposition formation increased as the blowing ratio (mass flux of cooling flow/mass flux of the mainstream flow) decreased from M=1.0, 0.25 and finally to 0.0 (no cooling). The study of the effects of the surface coating on deposition showed that TBC\u27s increased the rate of deposition over the exact same test article that was not coated. Instead of forming new deposits with twice the run time, the deposits started forming on top of other deposits showing that even at high particulate loadings the deposition did not affect the film cooling downstream of the cooling holes. Post test surface roughness scans were planned and performed on the test articles that didn\u27t have deposits break off as they were removed from the facility. Most test articles were not scanned due to the flyash deposits breaking or sluffing off as the test article cooled after the conclusion of the test. In contrast, the flyash deposits that formed on the interior (non-TBC coated) walls of the test section did not display any sluffing during cool down. This research is valuable to gas turbine manufacturers and operators to understand the variables that promote deposition so appropriate mitigations can be put in place to prevent engine downtime and component failures

Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli

The Escherichia coli uhp T gene encodes an active transport system for sugar phosphates. When the uhp T gene was carried on a multicopy plasmid, amplified levels of transport activity occurred, and growth of these strains was inhibited upon the addition of various sugar phosphates. Two different mechanisms for this growth inhibition were distinguished. Exposure to glucose-6-phosphate, fructose-6-phosphate or mannose-6-phosphate, which enter directly into the glycolytic pathway, resulted in cessation of growth and substantial loss of viability. Cell killing was correlated with the production of the toxic metabolite, methylglyoxal. In contrast, addition of 2-deoxyglucose-6-phosphate, galactose-6-phosphate, glucosamine-6-phosphate or arabinose-5-phosphate, which do not directly enter the glycolytic pathway, resulted in growth inhibition without engendering methylglyoxal production or cell death. Inhibition of growth could result from excessive accumulation of organophosphates in the cell or depletion of inorganic phosphate pools as a result of the sugar-P/Pi exchange process catalysed by UhpT. The phosphate-dependent uptake of glycerol-3-phosphate by the GlpT antiporter was strongly inhibited under conditions of elevated sugar-phosphate transport. There are thus two separate toxic effects of elevated sugar-phosphate transport, one of which was lethal and related to increased flux through glycolysis. It is likely that the control of uhpT transcription by catabolite repression exists to limit the level of UhpT transport activity and thereby prevent the toxic events that result from elevated uptake of its substrates

Exchange biased anomalous Hall effect driven by frustration in a magnetic kagome lattice.

Co[Formula: see text]Sn[Formula: see text]S[Formula: see text] is a ferromagnetic Weyl semimetal that has been the subject of intense scientific interest due to its large anomalous Hall effect. We show that the coupling of this material's topological properties to its magnetic texture leads to a strongly exchange biased anomalous Hall effect. We argue that this is likely caused by the coexistence of ferromagnetism and geometric frustration intrinsic to the kagome network of magnetic ions, giving rise to spin-glass behavior and an exchange bias

Maximum Independent Sets in Subcubic Graphs: New Results

The maximum independent set problem is known to be NP-hard in the class of subcubic graphs, i.e. graphs of vertex degree at most 3. We present a polynomial-time solution in a subclass of subcubic graphs generalizing several previously known results

High Frequency of Extra-Pair Paternity in Eastern Kingbirds

Genetic parentage in the socially monogamous and territorial Eastern Kingbird( Tyrannust tyrannus) was examined in a central New York population by multilocus DNA fingerprinting. Extra-pair young were identified in 60% (12 of 20) of nests. Of the 64 nestlings profiled, 42% were sired by extra-pair males, but no cases of conspecific brood parasitism were detected. These results are markedly different from a previous electrophoretic study of the same species in a Michigan population, which reported 39% of nestlings were unrelated to one (typically the mother, quasiparasitismo)r both (conspecificb roodp arasitism) of the putative parents. In the New York population, extra-pairp aternityw as most common among females that returned to breed on a former territory. Among females that were new to a breeding territory, extrapair paternity increased directly with breeding density. Although the power of the tests was low, neither breeding synchrony nor male experience with a breeding territory appeared to be associated with the occurrence of extra-pair young

The UVES Spectral Quasar Absorption Database (SQUAD) Data Release 1: The first 10 million seconds

We present the first data release (DR1) of the UVES Spectral Quasar Absorption Database (SQUAD), comprising 467 fully reduced, continuum-fitted high-resolution quasar spectra from the Ultraviolet and Visual Echelle Spectrograph (UVES) on the European Southern Observatory's Very Large Telescope. The quasars have redshifts $z=0$-5, and a total exposure time of 10 million seconds provides continuum-to-noise ratios of 4-342 (median 20) per 2.5-km/s pixel at 5500 \AA. The SQUAD spectra are fully reproducible from the raw, archival UVES exposures with open-source software, including our UVES_popler tool for combining multiple extracted echelle exposures which we document here. All processing steps are completely transparent and can be improved upon or modified for specific applications. A primary goal of SQUAD is to enable statistical studies of large quasar and absorber samples, and we provide tools and basic information to assist three broad scientific uses: studies of damped Lyman-$\alpha$ systems (DLAs), absorption-line surveys and time-variable absorption lines. For example, we provide a catalogue of 155 DLAs whose Lyman-$\alpha$ lines are covered by the DR1 spectra, 18 of which are reported for the first time. The HI column densities of these new DLAs are measured from the DR1 spectra. DR1 is publicly available and includes all reduced data and information to reproduce the final spectra.Comment: 21 pages, 18 figures. Accepted by MNRAS. All final quasar spectra, reduced contributing exposures, and supplementary material available via https://github.com/MTMurphy77/UVES_SQUAD_DR

Accurate Determination of Phenotypic Information from Historic Thoroughbred Horses by Single Base Extension

Historic DNA have the potential to identify phenotypic information otherwise invisible in the historical, archaeological and palaeontological record. In order to determine whether a single nucleotide polymorphism typing protocol based on single based extension (SNaPshot™) could produce reliable phenotypic data from historic samples, we genotyped three coat colour markers for a sample of historic Thoroughbred horses for which both phenotypic and correct geotypic information were known from pedigree information in the General Stud Book. Experimental results were consistent with the pedigrees in all cases. Thus we demonstrate that historic DNA techniques can produce reliable phenotypic information from museum specimens.© 2010 Campana et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

An electrophysiological signal that precisely tracks the emergence of error awareness

Recent electrophysiological research has sought to elucidate the neural mechanisms necessary for the conscious awareness of action errors. Much of this work has focused on the error positivity (Pe), a neural signal that is specifically elicited by errors that have been consciously perceived. While awareness appears to be an essential prerequisite for eliciting the Pe, the precise functional role of this component has not been identified. Twenty-nine participants performed a novel variant of the Go/No-go Error Awareness Task (EAT) in which awareness of commission errors was indicated via a separate speeded manual response. Independent component analysis (ICA) was used to isolate the Pe from other stimulus- and response-evoked signals. Single-trial analysis revealed that Pe peak latency was highly correlated with the latency at which awareness was indicated. Furthermore, the Pe was more closely related to the timing of awareness than it was to the initial erroneous response. This finding was confirmed in a separate study which derived IC weights from a control condition in which no indication of awareness was required, thus ruling out motor confounds. A receiver-operating-characteristic (ROC) curve analysis showed that the Pe could reliably predict whether an error would be consciously perceived up to 400 ms before the average awareness response. Finally, Pe latency and amplitude were found to be significantly correlated with overall error awareness levels between subjects. Our data show for the first time that the temporal dynamics of the Pe trace the emergence of error awareness. These findings have important implications for interpreting the results of clinical EEG studies of error processing