Artesanía, una producción local para mercados globales. El caso de Amatenango del Valle, Chiapas México

En este trabajo se examina el caso de la alfarería artesanal de Amatenango del Valle, Chiapas con el objetivo de plantear posibilidades para alentar la producción de artesanía, entendida como oficio comunitario y local, hacia mercados globales

New VME indicator species (excluding corals and sponges) and some potential VME elements of the NAFO Regulatory Area

A review of over 500 taxa known to occur in the NRA revealed three additional faunal groups (additional to NAFO 2008a) that meet the criteria for a VME indicator based on traits related to functional significance, fragility, and the life-history traits of component species that produce a slow recovery to disturbance. These are crinoids, erect bryozoans and large sea squirts. For each group it is the dense aggregations (beds/fields) that are considered to be VME in order to establish functional significance. Although each group is present in the NRA, data to date have not revealed any concentrations of note with the exception of one catch of the stalked tunicate Boltenia ovifera (large sea squirt). Black corals were considered to be VME indicators by NAFO based on what was then known of their distribution. These were included based on the uniqueness/rarity criterion of habitats of rare, threatened or endangered species that occur only in discrete areas, however evaluation of their distribution using trawl survey, rock dredge and underwater video has indicated that they have widespread occurrence at low densities in the NRA and along the continental slopes off Labrador. However, because they are thought to be extremely long-lived and therefore ‘iconic’ if not rare, we have identified where the highest frequency of occurrence is for this taxon. Lastly, based on NEREIDA multibeam bathymetry we have identified more canyon heads, steep flanks and new seamounts in the NRA as possible VME elements

Transformación vía Agrobacterium tumefaciens para inducir tolerancia a la podredumbre blanca del aguacate

Comunicación presentada en el VII World Avocado Congress, celebrado en Cairns (Australia) del 5 al 9 de septiembre de 2011.[EN] One of the most important limiting factors for avocado production in Spain is the disease caused by the fungus Rosellinia necatrix . Genetic manipulation could be useful for the introduction of fungal resistance traits into this crop. A n efficient Agrobacterium - mediated transformation protocol for avocado using AGL1 Agrobacterium strain and somatic embryos as the target material has been established by our group, although embryo conversion rate into plants needs to be improved. For that reason, we are using the strawberry, another Rosellinia necatrix ́s host, as model species to test the effect of several transgenes (two of fungal origin, chit 42 chitinase and β - 1,3 - glucanase from Trichoderma harzianum , and one of plant origin, At NPR1), on inducing tolerance to this fungus. Strawberry transformation with the β - 1,3 - glucanase gene has allowed the selection of two lines, β6 and β10, with enhanced tolerance to R. necatrix while no positive results were obtained following transformation with the chit - 42 gene. In relation to the At NPR1 gene more than 30 independent transgenic lines have been obtained whose tolerance to R. necatrix is currently under evaluation. Concerning avocado transformation, more than 10 independent transgenic lines (derive d from an embryogenic line of an immature Duke7 zygotic embryo) have been obtained with At NPR1 gene. Plants have been recovered from one line and efforts are underway to recover plants from other lines following micrografting of the transgenic sprouted sho ots onto in vitro germinated seedlings.[ES] Uno de los factores limitantes de la producción de aguacate en España es la enfermedad causada por el hongo R. necatrix. La manipulación genética podría ser de utilidad para introducir caracteres de resistencia en este cultivo. Se ha establecido un sistema eficiente de transformación en aguacate usando la cepa de Agrobacterium AGL1 y células embriogénicas como diana, sin embargo, la conversión en plantas de los embriones transgénicos necesita ser mejorada. Por esta razón, estamos utilizando la fresa, otro huésped de R. necatrix, como especie modelo para testar el efecto de varios transgenes (2 de origen fúngico, la quitinasa chit-42 y la β-1,3-glucanasa de Trichoderma harzianum, y uno derivado de plantas, AtNPR1), en la inducción de tolerancia a este patógeno tras la transformación de esta especie. La transformación de fresa con el gen de β -1,3-glucanasa ha permitido la selección de dos líneas, β6 y β10, con mayor tolerancia a R. necatrix, mientras que no se han obtenido resultados positivos con el gen chit-42. En relación con el gen AtNPR1, se han obtenido más de 30 líneas transgénicas independientes, cuya tolerancia frente a R. necatrix se está evaluando en la actualidad. En relación con la transformación de aguacate, más de 10 líneas transgénicas independientes (derivadas de una línea embriogénica obtenida a partir de un embrión zigótico inmaduro del cv. Duke 7) se han obtenido con el gen AtNPR1. Se han recuperado plantas de una línea y actualmente se está intentando recuperar plantas de otras líneas mediante microinjerto de los embriones transgénicos germinados.Este trabajo se ha realizado en el marco del proyecto AGL2008 - 05453 - C02 - 01/AGR.Peer Reviewe

Transformación genética de olivo (Olea europaea L.) con un gen de Medicago truncatula que codifica para un proteína tipo FT

En el olivo (Olea europaea L.), la modificación de la arquitectura de la planta y la reducción del periodo juvenil son caracteres de interés en la mejora. Las proteínas codificadas por genes tipo FT (FLOWERING LOCUS T), además de actuar como componente principal de la señal sistémica inductora de floración conocida como florígeno, participan en la regulación de otros procesos del desarrollo en plantas, entre ellos, la determinación de la arquitectura o la dormancia. El objetivo de este trabajo es abordar la transformación genética de olivo con el gen MtFTa1 de Medicago truncatula, para estudiar su aplicación en la mejora. La transformación genética se llevó a cabo utilizando embriones somáticos, derivados de radícula de embrión zigótico, siguiendo el protocolo previamente establecido en nuestro laboratorio (Torreblanca et al. 2010, PCTOC 103:61-69). Se utilizaron la cepa de Agrobacterium AGL-1 y el vector binario Pro35S:MtFTA portando el gen nptII como gen de selección y el gen MtFTa1 bajo el control del promotor constitutivo CaMV35S. La regeneración de plantas se realizó siguiendo el protocolo previamente desarrollado en nuestro grupo de trabajo (Cerezo et al. 2011, PCTOC 106:337-344). Se obtuvo una tasa de transformación del 2,5%, recuperándose quince líneas transgénicas independientes. La expresión del transgén se analizó mediante qRT-PCR. En tres de las seis líneas con mayores niveles de expresión se observó floración precoz in vitro, mientras que las otras tres líneas transgénicas florecieron en invernadero de confinamiento, transcurridos 18-36 meses desde su aclimatación. Las flores obtenidas presentaron morfología irregular y no produjeron polen viable. Además, las plantas mostraron alteraciones en el crecimiento, como pérdida de dominancia apical y desarrollo continuo de yemas laterales. Por otro lado, en plantas aclimatadas que no florecieron tan precozmente, también se observó un mayor grado de ramificación en el eje principal en relación a las plantas control, con una menor longitud de entrenudos y mayor porcentaje de yemas axilares brotadas en ramos laterales de primer orden. Los resultados de este trabajo ponen de manifiesto el papel del gen FT en la regulación de la floración y arquitectura de las plantas de olivo.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Proyecto de Excelencia Junta de Andalucía P11-AGR-7992

Isogenic GAA-KO Murine Muscle Cell Lines Mimicking Severe Pompe Mutations as Preclinical Models for the Screening of Potential Gene Therapy Strategies

Pompe disease (PD) is a rare disorder caused by mutations in the acid alpha-glucosidase (GAA) gene. Most gene therapies (GT) partially rely on the cross-correction of unmodified cells through the uptake of the GAA enzyme secreted by corrected cells. In the present study, we generated isogenic murine GAA-KO cell lines resembling severe mutations from Pompe patients. All of the generated GAA-KO cells lacked GAA activity and presented an increased autophagy and increased glycogen content by means of myotube differentiation as well as the downregulation of mannose 6-phosphate receptors (CI-MPRs), validating them as models for PD. Additionally, different chimeric murine GAA proteins (IFG, IFLG and 2G) were designed with the aim to improve their therapeutic activity. Phenotypic rescue analyses using lentiviral vectors point to IFG chimera as the best candidate in restoring GAA activity, normalising the autophagic marker p62 and surface levels of CI-MPRs. Interestingly, in vivo administration of liver-directed AAVs expressing the chimeras further confirmed the good behaviour of IFG, achieving cross-correction in heart tissue. In summary, we generated different isogenic murine muscle cell lines mimicking the severe PD phenotype, as well as validating their applicability as preclinical models in order to reduce animal experimentation.Fundacion Poco Frecuente (Almeria)Asociacion Espanola de Enfermos de Glucogenosis (AEEG)Asociacion Espanola de Enfermos de Pompe (AEEP

A first approach to assess the impact of bottom trawling over Vulnerable Marine Ecosystems on the High Seas of the Southwest Atlantic

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The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray.

Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited mayor que50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy

Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study.

The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens

Informe de la Campaña ARGELIA0203-DP para la evaluación de recursos demersales profundos en las costas de Argelia (Mediterráneo Sud-Occidental)

RESUMEN: Del 5 al 24 de marzo de 2003 se llevó a cabo la campaña de prospección pesquera con arrastre de fondo ARGELIA0203-DP en aguas de Argelia, a bordo del B/O Vizconde de Eza. Se realizaron 55 pescas (3 nulas) entre 200 y 800 m de profundidad. Se presentan los resultados de las capturas realizadas en esta campaña y se analizan los índices de abundancia y biomasa de las especies más importantes, por sector y área geográficos y estrato batimétrico, así como su distribución de tallas por sector geográfico y estrato batimétrico. Se comparan los rendimientos y las tallas obtenidos con los de otras campañas realizadas en el Mediterráneo (MEDITS y BALAR)RÉSUME: Rapport de la campagne ARGELIA0203-DP portant evaluation des ressources demersales des côtes Algeriennes (Mediterrane sud-occidental).- Du 05 au 24 Mars 2003, s’est réalisée une campagne de prospection par chalutage du fond ARGELIA0203-DP des côtes Algériennes, abord du B/O Vizconde de Eza. 55 traits de pêche (3 annulées) ont été effectue entre 200 et 800 m de profondeur. Les résultats des captures réalisées durant cette campagne et l’analyse des indices d’abondance et biomasse des espèces les plus importantes sont présentés, par secteur, par aire géographique et strate bathymétrique, ainsi que leurs distributions de tailles par secteur géographique et strate bathymétrique. Les rendements et les tailles obtenus ont fait l’objet d’une comparaison avec les autres campagnes réalisées dans la Méditerranée (MEDITS et BALAR)ABSTRACT: Report of the ARGELIA0203-DP survey for the assessment of the deep-sea demersal resources off Algerian coasts (southwestern Mediterranean).- From March 5th to 24th 2003 the bottom-trawl survey ARGELIA0203-DP was developed in waters off Argelia, on board R/V Vizconde de Eza. Fifty-five hauls (3 null) were carried out between 200 and 800 m depth. The results of the captures obtained during this survey are shown and abundance and biomass indexes of the most important species are analyzed by geographic sector and area, and bathymetric stratum, as their size distribution by geographic sector and bathymetric stratum. Yields and sizes obtained are compared to those from other surveys carried out in the Mediterranean (MEDITS and BALAR)

Prediction of poor outcome in clostridioides difficile infection: A multicentre external validation of the toxin B amplification cycle

Producción CientíficaClassification of patients according to their risk of poor outcomes in Clostridioides difficile infection (CDI) would enable implementation of costly new treatment options in a subset of patients at higher risk of poor outcome. In a previous study, we found that low toxin B amplification cycle thresholds (Ct) were independently associated with poor outcome CDI. Our objective was to perform a multicentre external validation of a PCR-toxin B Ct as a marker of poor outcome CDI. We carried out a multicentre study (14 hospitals) in which the characteristics and outcome of patients with CDI were evaluated. A subanalysis of the results of the amplification curve of real-time PCR gene toxin B (XpertTM C. difficile) was performed. A total of 223 patients were included. The median age was 73.0 years, 50.2% were female, and the median Charlson index was 3.0. The comparison of poor outcome and non–poor outcome CDI episodes revealed, respectively, the following results: median age (years), 77.0 vs 72.0 (p = 0.009); patients from nursing homes, 24.4% vs 10.8% (p = 0.039); median leukocytes (cells/μl), 10,740.0 vs 8795.0 (p = 0.026); and median PCR-toxin B Ct, 23.3 vs 25.4 (p = 0.004). Multivariate analysis showed that a PCR-toxin B Ct cut-off <23.5 was significantly and independently associated with poor outcome CDI (p = 0.002; OR, 3.371; 95%CI, 1.565–7.264). This variable correctly classified 68.5% of patients. The use of this microbiological marker could facilitate early selection of patients who are at higher risk of poor outcome and are more likely to benefit from newer and more costly therapeutic options