Age and sexual features of stomatologic incidence at patients of clinics of various forms of ownership

Preservation of stomatologic health at persons of able-bodied age extremely topical issue. Today the competition of the state and private odontology became objective reality. During research of the stomatologic status of inhabitants of the monotown of Chelyabinsk region it is confirmed that higher appealability of the patients preferring treatment in paid clinic, is not a consequence of their inferior stomatologic status. But it is accompanied by improvement of this status, at the expense of well-timed sanitation of a painful teeth.Сохранение стоматологического здоровья у лиц трудоспособного возраста чрезвычайно актуальный вопрос. Сегодня объективной реальностью стала конкуренция государственной и частной стоматологии. В ходе исследования стоматологического статуса жителей моногорода Челябинской области подтверждено, что более высокая обращаемость пациентов, предпочитающих лечение в платной клинике, не является следствием их худшего стоматологического статуса. Но сопровождается улучшением этого статуса, за счёт своевременной санации больных зубов

Evaluation of the morphonctional status and a series of biochemical parameters of thromboconcentrate during storage

The purpose of the study is to establish whether there are changes in the morphofunctional status and biochemical parameters during the storage of thromboconcentrate.Цель исследования – установить имеются ли изменения морфофункционального статуса и биохимических параметров при хранении тромбоконцентрата

Clinical and diagnostic significance of some laboratory analysis of pericardial fluid

The aim of the study – identify differential laboratory characteristic of pericardial fluid examination in exudative and metastatic pericarditis.Цель исследования – установить дифференциальные лабораторные признаки исследования ПЖ при воспалительном и метастатическом перикардите

Sociological assessment of the quality of medical care in the clinic of the municipal formation

Quality assurance of medical care is the most urgent problem of the national healthcare system. The aim of the work was the estimation of the quality of medical care in municipal polyclinic by questioning patients. Prepared the original form with 9 questions. On completing the substantial and the General part of the questionnaire can be noted that most of the patients were often visited by the medical institution and have an understanding of his work and a fully formed opinion about the quality of medical assistance it received. Conducted by the sociological survey, the analysis of its results were an important channel of information for managers of health care institutions allows a quick assessment of the activities of the organization; measure the ratio of patients to health workers, conditions of delivery and services provided. The changes taking place in the health care system at the present time, have a positive response among the population.Обеспечение качества оказания медицинской помощи является актуальнейшей проблемой отечественного здравоохранения. Целью работы явилась оценка качества медицинской помощи в муниципальной поликлинике путем анкетирования пациентов. Подготовлен оригинальный бланк с 9 вопросами. По заполнению констатирующей и общей части анкеты можно отметить, что основная часть пациентов достаточно часто посещают исследуемое медицинское учреждение и имеют представление о его работе и вполне сложившееся мнение о качестве медицинской помощи в ней получаемой. Проведенное социологическое исследование, анализ его результатов явились важным информационным каналом для руководителей учреждения здравоохранения позволяющий оперативно получить оценку деятельности организации; измерить отношение пациентов к медработникам, условиям предоставления и предоставляемым услугам. Изменения, происходящие в системе здравоохранения в настоящее время, имеют положительный отклик у обслуживаемого населения

Stratification of asthma phenotypes by airway proteomic signatures

© 2019 Background: Stratification by eosinophil and neutrophil counts increases our understanding of asthma and helps target therapy, but there is room for improvement in our accuracy in prediction of treatment responses and a need for better understanding of the underlying mechanisms. Objective: We sought to identify molecular subphenotypes of asthma defined by proteomic signatures for improved stratification. Methods: Unbiased label-free quantitative mass spectrometry and topological data analysis were used to analyze the proteomes of sputum supernatants from 246 participants (206 asthmatic patients) as a novel means of asthma stratification. Microarray analysis of sputum cells provided transcriptomics data additionally to inform on underlying mechanisms. Results: Analysis of the sputum proteome resulted in 10 clusters (ie, proteotypes) based on similarity in proteomic features, representing discrete molecular subphenotypes of asthma. Overlaying granulocyte counts onto the 10 clusters as metadata further defined 3 of these as highly eosinophilic, 3 as highly neutrophilic, and 2 as highly atopic with relatively low granulocytic inflammation. For each of these 3 phenotypes, logistic regression analysis identified candidate protein biomarkers, and matched transcriptomic data pointed to differentially activated underlying mechanisms. Conclusion: This study provides further stratification of asthma currently classified based on quantification of granulocytic inflammation and provided additional insight into their underlying mechanisms, which could become targets for novel therapies

Plasma proteins elevated in severe asthma despite oral steroid use and unrelated to Type-2 inflammation

Rationale Asthma phenotyping requires novel biomarker discovery. Objectives To identify plasma biomarkers associated with asthma phenotypes by application of a new proteomic panel to samples from two well-characterised cohorts of severe (SA) and mild-to-moderate (MMA) asthmatics, COPD subjects and healthy controls (HCs). Methods An antibody-based array targeting 177 proteins predominantly involved in pathways relevant to inflammation, lipid metabolism, signal transduction and extracellular matrix was applied to plasma from 525 asthmatics and HCs in the U-BIOPRED cohort, and 142 subjects with asthma and COPD from the validation cohort BIOAIR. Effects of oral corticosteroids (OCS) were determined by a 2-week, placebo-controlled OCS trial in BIOAIR, and confirmed by relation to objective OCS measures in U-BIOPRED. Results In U-BIOPRED, 110 proteins were significantly different, mostly elevated, in SA compared to MMA and HCs. 10 proteins were elevated in SA versus MMA in both U-BIOPRED and BIOAIR (alpha-1-antichymotrypsin, apolipoprotein-E, complement component 9, complement factor I, macrophage inflammatory protein-3, interleukin-6, sphingomyelin phosphodiesterase 3, TNF receptor superfamily member 11a, transforming growth factor-β and glutathione S-transferase). OCS treatment decreased most proteins, yet differences between SA and MMA remained following correction for OCS use. Consensus clustering of U-BIOPRED protein data yielded six clusters associated with asthma control, quality of life, blood neutrophils, high-sensitivity C-reactive protein and body mass index, but not Type-2 inflammatory biomarkers. The mast cell specific enzyme carboxypeptidase A3 was one major contributor to cluster differentiation. Conclusions The plasma proteomic panel revealed previously unexplored yet potentially useful Type-2independent biomarkers and validated several proteins with established involvement in the pathophysiology of SA

Epithelial IL-6 trans-signaling defines a new asthma phenotype with increased airway inflammation

Background: Although several studies link high levels of IL-6 and soluble IL-6 receptor (sIL-6R) to asthma severity and decreased lung function, the role of IL-6 trans-signaling (IL-6TS) in asthmatic patients is unclear. Objective: We sought to explore the association between epithelial IL-6TS pathway activation and molecular and clinical phenotypes in asthmatic patients. Methods: An IL-6TS gene signature obtained from air-liquid interface cultures of human bronchial epithelial cells stimulated with IL-6 and sIL-6R was used to stratify lung epithelial transcriptomic data (Unbiased Biomarkers in Prediction of Respiratory Disease Outcomes [U-BIOPRED] cohorts) by means of hierarchical clustering. IL-6TS-specific protein markers were used to stratify sputum biomarker data (Wessex cohort). Molecular phenotyping was based on transcriptional profiling of epithelial brushings, pathway analysis, and immunohistochemical analysis of bronchial biopsy specimens. Results: Activation of IL-6TS in air-liquid interface cultures reduced epithelial integrity and induced a specific gene signature enriched in genes associated with airway remodeling. The IL-6TS signature identified a subset of patients with IL-6TS-high asthma with increased epithelial expression of IL-6TS-inducible genes in the absence of systemic inflammation. The IL-6TS-high subset had an overrepresentation of frequent exacerbators, blood eosinophilia, and submucosal infiltration of T cells and macrophages. In bronchial brushings Toll-like receptor pathway genes were upregulated, whereas expression of cell junction genes was reduced. Sputum sIL-6R and IL-6 levels correlated with sputum markers of remodeling and innate immune activation, in particular YKL-40, matrix metalloproteinase 3, macrophage inflammatory protein 1 beta, IL-8, and IL-1 beta. Conclusions: Local lung epithelial IL-6TS activation in the absence of type 2 airway inflammation defines a novel subset of asthmatic patients and might drive airway inflammation and epithelial dysfunction in these patients.Peer reviewe

A computational framework for complex disease stratification from multiple large-scale datasets.

BACKGROUND: Multilevel data integration is becoming a major area of research in systems biology. Within this area, multi-'omics datasets on complex diseases are becoming more readily available and there is a need to set standards and good practices for integrated analysis of biological, clinical and environmental data. We present a framework to plan and generate single and multi-'omics signatures of disease states. METHODS: The framework is divided into four major steps: dataset subsetting, feature filtering, 'omics-based clustering and biomarker identification. RESULTS: We illustrate the usefulness of this framework by identifying potential patient clusters based on integrated multi-'omics signatures in a publicly available ovarian cystadenocarcinoma dataset. The analysis generated a higher number of stable and clinically relevant clusters than previously reported, and enabled the generation of predictive models of patient outcomes. CONCLUSIONS: This framework will help health researchers plan and perform multi-'omics big data analyses to generate hypotheses and make sense of their rich, diverse and ever growing datasets, to enable implementation of translational P4 medicine

Rare Case of Intraocular Dirofiliariosis. Surgical Removing Method

Dirofilariasis is a biohelminthiasis characterized by damage to the hypodermis, less often than internal organs, in about 50 % of cases localized under the eye membranes, adnexa or in the orbit; the clinical symptoms depend on the location of the parasite infestation. This is a serious disease that can cause dangerous somatic complications. Currently, there is an increase in the incidence of dirofilariasis and the “expansion” of its endemic zones to the north. Most often, the parasite develops at the site of a mosquito bite — subcutaneously, it determines the subsequent clinical manifestations. A lot of questions cause the development of the nematode in the internal structures of the eye, as well as the pathway of penetration of the parasite. The literature describes isolated cases of intraocular invasion of dirofilariae, for example, in Russia since 1972, only three such cases have been recorded. This article presents a clinical case of intravitreal dirofilariasis and presents a method for its surgical treatment. Currently, there is no single approach to removing dirofilaria from the vitreous cavity due to the rare occurrence of this pathology. The high probability of the inflammatory reaction associated with the presence of the xenoagent, its fragmentation during the manipulation, as well as the potential decrease in visual acuity, determined the purpose of this work — to develop a method for surgical removal of the worm from the eye cavity in intact form using a closed manual aspiration system via 2-port access 25G without vitrectomy for further identification in the parasitological department of the clinical diagnostic laboratory