2,363 research outputs found
Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ
A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow, have prompted several groups to develop freeware systems to perform certain steps of gel analysis. Unfortunately, to the best of our knowledge none of them offer a package that performs all the steps envisaged in a 2D-GE gel analysis. Here we describe an ImageJ-based procedure, able to manage all the steps of a 2D-GE gel analysis. ImageJ is a free available image processing and analysis application developed by National Institutes of Health (NIH) and widely used in different life sciences fields as medical imaging, microscopy, western blotting and PAGE. Nevertheless no one has yet developed a procedure enabled to compare spots on 2D-GE gels. We collected all used ImageJ tools in a plug-in that allows us to perform the whole 2D-GE analysis. To test it, we performed a set of 2D-GE experiments on plasma samples from 9 patients victims of acute myocardial infarction and 8 controls, and we compared the results obtained by our procedure to those obtained using a widely diffuse commercial package, finding similar performance
More on microstate geometries of 4d black holes
We construct explicit examples of microstate geometries of four-dimensional
black holes that lift to smooth horizon-free geometries in five dimensions.
Solutions consist of half-BPS D-brane atoms distributed in .
Charges and positions of the D-brane centers are constrained by the bubble
equations and boundary conditions ensuring the regularity of the metric and the
match with the black hole geometry. In the case of three centers, we find that
the moduli spaces of solutions includes disjoint one-dimensional components of
(generically) finite volume.Comment: 25 page
Is the team leading surgeon criminally liable for his collaborators’ errors? judges confirm responsibility and condemn an otorhinolaryngologist
In current healthcare, delivery of medical and surgical treatment takes place in a multidisciplinary manner. This raises the problem of distinguishing the conditions under which the person who has properly carried out his duties, respecting the related leges artis, can be held responsible for damages materially caused by another member of the medical team. Jurisprudence has developed the so-called “principle of trust” for which every member of the team can rely on the fact that other members are acting in compliance with the leges artis of their specialisation. The Supreme Court has limited the application of this principle. The authors examine the jurisprudence on responsibility of the team in otolaryngology and conclude that individual liability should be limited to the specific expertise of the individual specialist
An atypical case of trigeminal trophic syndrome: a legal medicine perspective in medical responsibility
BACKGROUND: Trigeminal trophic syndrome is a rare complication of peripheral or central damage to the trigeminal nerve characterized by anesthesia, paresthesia and a secondary persistent facial ulceration. METHODS: We describe the case of a 40-year-old woman with previous history of Le Fort I osteotomy for a class III malocclusion who developed trigeminal trophic syndrome. Atypically, the cutaneous symptoms appeared bilaterally and 8 years after surgery. RESULTS: Differential diagnosis was based on clinical history, tissue biopsy and serologic evaluation. Atypical findings could be linked to the surgical burdens of Le Fort I osteotomy, a procedure characterized by a bilateral incision on the maxillofacial bones with a reasonable probability of causing a bilateral injury of the peripheral branches of the trigeminal nerve. CONCLUSION: Although the long delay between trigeminal trophic syndrome onset and surgery and the absence of adequate medical evidence cannot confirm a link with previous surgery in this case, the increasing number of maxillofacial surgery cases suggests that this complication may be more frequent in the next decades, and thus, involved specialists should be aware of this condition as a possible complication of maxillofacial surgery procedures
Per il Dante di Fernando Bandini
Nella poesia di Fernando Bandini la memoria di Dante ha una lunga durata, dagli anni Sessanta fino alle Quattordici poesie pubblicate nel 2010. Il presente intervento esamina nel suo complesso, per la prima volta, il dantismo di Bandini, attraverso alcuni significativi campioni che si presentano come recuperi di situazioni dantesche, espliciti tasselli intertestuali o riscritture di scene pi\uf9 ampie. Il ruolo giocato dalla Commedia appare come quello di una miniera di materiali liberamente citabili e manipolabili, senza che l\u2019io poetico subisca alcuna \u201cangoscia dell\u2019influenza\u201d da parte del modello
Il muro di Sereni: una lettura
L’articolo intende offrire una lettura de Il muro analizzandone il profilo stilistico e tematico, mettendo il testo in relazione con altri momenti significativi della poesia di Sereni – per esempio da Frontiera – e con alcuni riscontri intertestuali importanti per la sua elaborazione (da Montale, Sbarbaro, ecc.). Imperniato su una sorta di generale principio della ripetizione e sull’unione fertile fra memoria letteraria e esperienza del soggetto, Il muro si mostra centrale nel percorso sereniano per il riproporsi del tema classico dell’apparizione del defunto, qui nelle forme dell’incontro fra pater e puer: una ‘scena primaria’ che viene ricondotta al modello di Rilke, Dante e Baudelaire, fino alla ridefinizione della funzione paterna come figura di ‘senso’, ben oltre i confini del meccanismo edipico freudiano
Olfactory Delusional Syndrome and Intracranial Meningioma
We report the case of a 37-year-old female in which the removal of a suprasellar neoplasm was accompanied by the disappearance of a longstanding olfactory delusion syndrome. In primary care the patient condition was exclusively thought to be psychic in origin, neglecting the possible, not infrequent, organic contribution. The delayed diagnosis produced neurological impairment, only partially recovered after surgical therapy. This case might help to improve the patient management via multi-specialist cooperation and to broaden the knowledge about somatic mechanisms of psychic disturbances, are not often taken into account
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