Trace-metaldynamics in response of increase CO₂ and iron availability in a coastal mesocosm experiment

A mesocosm experiment was performed in the Raunefjord (Norway) to study changes in dissolved Cu (dCu) and Fe (dFe), and in the elemental composition of particles during an Emiliania huxleyi dominated bloom. The CO2 treatments consisted of present (LC; 390 ppmV) and predicted levels (HC; 900 ppmV) and iron conditions were created with the addition of the siderophore desferoxamine B (DFB). Our results showed the DFB addition enhanced the solubility of Fe in this fjord environment. Initially, dFe was comparable among treatments but after the addition, the HC and/or +DFB treatments presented higher levels and finally, the only ones maintaining high dFe were the +DFB treatments. Unlike dCu presented indistinguishable levels in all mesocosms over time. Particulate metals were normalised to P and Al to evaluate the relative influence of biotic and abiotic sources. The Fe:P ratios decreased with time and compared to published phytoplankton ratios suggest Fe storage. On the other hand, Fe:Al ratios were relatively closer to the crustal ratios suggesting that the abiotic source was more important for this metal. Trends for other metals will be discussed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

The effects of ocean acidification and siderophore additions on trace-metal dynamics during an Emiliania Huxleyi- dominated bloom in a coastal mesocosm experiment

The combined impacts of high CO2 and changes in trace metal availability on plankton remain largely unknown. A 22 days mesocosm experiment was carried out in the Raunefjord, off Bergen, Norway (June 2012) to investigate the interactive effects of increased CO2 and changes in iron availability in the pelagic community. Twelve mesocosms covered by PAR and UVR transparent lids were used. The CO2 treatments consisted of present (LC; 390 ppmV) and predicted levels for year 2100 (HC; 900 ppmV). On day 7, half of the mesocoms were amended with the siderophore desferoxamine B (DFB) at 70 nM (final concentration). We studied changes in dissolved Cu (dCu) and Fe (dFe), and in the elemental composition of particles. Dissolved Fe and dCu were measured by chemiluminescence flow injection analysis, while particulate metals were determined by high-resolution inductively coupled mass spectrometry. The metals were normalised to P and Al to evaluate the relative influence of biotic and abiotic sources. Samples were taken throughout a bloom of the coccolithophore E. huxleyi. Further details will be discussed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Efficient metallic spintronic emitters of ultrabroadband terahertz radiation

Terahertz electromagnetic radiation is extremely useful for numerous applications such as imaging and spectroscopy. Therefore, it is highly desirable to have an efficient table-top emitter covering the 1-to-30-THz window whilst being driven by a low-cost, low-power femtosecond laser oscillator. So far, all solid-state emitters solely exploit physics related to the electron charge and deliver emission spectra with substantial gaps. Here, we take advantage of the electron spin to realize a conceptually new terahertz source which relies on tailored fundamental spintronic and photonic phenomena in magnetic metal multilayers: ultrafast photo-induced spin currents, the inverse spin-Hall effect and a broadband Fabry-P\'erot resonance. Guided by an analytical model, such spintronic route offers unique possibilities for systematic optimization. We find that a 5.8-nm-thick W/CoFeB/Pt trilayer generates ultrashort pulses fully covering the 1-to-30-THz range. Our novel source outperforms laser-oscillator-driven emitters such as ZnTe(110) crystals in terms of bandwidth, terahertz-field amplitude, flexibility, scalability and cost.Comment: 18 pages, 10 figure

Remote Sensing Temporal Reconstruction of the Flooded Area in "Tablas de Daimiel" Inland Wetland 2000-2021

[EN] Tablas de Daimiel National Park (TDNP) is a unique inland wetland located in the Mancha plain (Spain). It is recognized at the international level, and it is protected by different figures, such as Biosphere Reserve. However, this ecosystem is endangered due to aquifer overexploitation, and it is at risk of losing its protection figures. The objective of our study is to analyze the evolution of the flooded area between the year 2000 and 2021 by Landsat (5, 7 and 8) and Sentinel-2 images, and to assess the TDNP state through an anomaly analysis of the total water body surface. Several water indices were tested, but the NDWI index for Sentinel-2 (threshold -0.20), the MNDWI for Landsat-5 (threshold -0.15), and the MNDWI for Landsat-8 (threshold -0.25) showed the highest accuracy to calculate the flooded surface inside the protected area's limits. During the period 2015-2021, we compared the performance of Landsat-8 and Sentinel-2 and an R2 value of 0.87 was obtained for this analysis, indicating a high correspondence between both sensors. Our results indicate a high variability of the flooded areas during the analyzed period with significant peaks, the most notorious in the second quarter of 2010. Minimum flooded areas were observed with negative precipitation index anomalies since fourth quarter of 2004 to fourth quarter of 2009. This period corresponds to a severe drought that affected this region and caused important deterioration. No significant correlation was observed between water surface anomalies and precipitation anomalies, and the significant correlation with flow and piezometric anomalies was moderate. This can be explained because of the complexity of water uses in this wetland, which includes illegal wells and the geological heterogeneity.Pena-Regueiro, J.; Estornell Cremades, J.; Aguilar-Maldonado, J.; Sebastiá-Frasquet, M. (2023). Remote Sensing Temporal Reconstruction of the Flooded Area in "Tablas de Daimiel" Inland Wetland 2000-2021. Sensors. 23(8). https://doi.org/10.3390/s2308409623

Surface Morphology and Electrical Resistivity in Polycrystalline Au/Cu/Si(100) System

This work describes the analysis of morphology and electrical resistivity (ρ) obtained in the Au/Cu/Si system. The Au/Cu bilayers were deposited by thermal evaporation technique with thicknesses from 50 to 250 nm on SiOx/Si(100) substrates. The Au : Cu concentration ratio of the samples was of 25 : 75 at%. The bilayers were annealed into a vacuum oven with argon atmosphere at 660 K for one hour. The crystalline structures of AuCu and CuSi alloys were confirmed by X-ray diffraction analysis. The scanning electron microscopy (SEM), the atomic force microscopy (AFM), and the energy dispersive spectroscopy (EDS) were used to study the morphology, final thickness, and the atomic concentration of the alloys formed, respectively. The four-point probe technique was used to measure the electrical resistivity (ρ) in the prepared alloys as a function of thickness. The ρ value was measured and it was numerically compared with the Fuchs–Sondheimer (FS) and the Mayadas–Shatzkes (MS) models of resistivity. Results show values of electrical resistivity between 0.9 and 1.9 μΩ-cm. These values are four times smaller than the values of the AuCu systems reported in literature

Using 67Cu to Study the Biogeochemical Cycling of Copper in the Northeast Subarctic Pacific Ocean

Microbial copper (Cu) nutrition and dissolved Cu speciation were surveyed along Line P, a coastal to open ocean transect that extends from the coast of British Columbia, Canada, to the high-nutrient-low-chlorophyll (HNLC) zone of the northeast subarctic Pacific Ocean. Steady-state size fractionated Cu uptake rates and Cu:C assimilation ratios were determined at in situ Cu concentrations and speciation using a 67Cu tracer method. The cellular Cu:C ratios that we measured (~30 µmol Cu mol C-1) are similar to recent estimates using synchrotron x-ray fluorescence (SXRF), suggesting that the 67Cu method can determine in situ metabolic Cu demands. We examined how environmental changes along the Line P transect influenced Cu metabolism in the sub-microplankton community. Cellular Cu:C assimilation ratios and uptake rates were compared with net primary productivity, bacterial abundance and productivity, total dissolved Cu, Cu speciation, and a suite of other chemical and biological parameters. Total dissolved Cu concentrations ([Cu]d) were within a narrow range (1.46 to 2.79 nM), and Cu was bound to a ~5-fold excess of strong ligands with conditional stability constants ( ) of ~1014. Free Cu2+ concentrations were low (pCu 14.4 to 15.1), and total and size fractionated net primary productivity (NPPV; µg C L-1 d-1) were negatively correlated with inorganic Cu concentrations ([Cu′]). We suggest this is due to greater Cu′ drawdown by faster growing phytoplankton populations. Using the relationship between [Cu′] drawdown and NPPV, we calculated a regional photosynthetic Cu:C drawdown export ratio between 1.5 and 15 µmol Cu mol C-1, and a mixed layer residence time (2.5 to 8 years) that is similar to other independent estimates (2-12 years). Total particulate Cu uptake rates were between 22 and 125 times faster than estimates of Cu export; this is possibly mediated by rapid cellular Cu uptake and efflux by phytoplankton and bacteria or the effects of grazers and bacterial remineralization on dissolved Cu. These results provide a more detailed understanding of the interactions between Cu speciation and microorganisms in seawater, and present evidence that marine phytoplankton modify Cu speciation in the open ocean

Calpain restrains the stem cells compartment in breast cancer

CAPNS1 is essential for the stability and function of ubiquitous CAPN1 and CAPN2. Calpain modulates by proteolytic cleavage many cellular substrates and its activity is often deregulated in cancer cells, therefore calpain inhibition has been proposed as a therapeutical strategy for a number of malignancies. Here we show that CAPNS1 depletion is coupled to impairment of MCF7 and MCF10AT cell lines growth on plate and defective architecture of mammary acini derived from MCF10A cells. In soft agar CAPNS1 depletion leads to cell growth increase in MCF7, and decrease in MCF10AT cells. In both MCF7 and MCF10AT, CAPNS1 depletion leads to the enlargement of the stem cell compartment, as demonstrated by mammosphere formation assays and evaluation of stem cell markers by means of FACS and western blot analysis. Accordingly, activation of calpain by thapsigargin treatment leads to a decrease in the stem cell reservoir. The expansion of the cancer stem cell population in CAPNS1 depleted cells is coupled to a defective shift from symmetric to asymmetric division during mammosphere growth coupled to a decrease in NUMB protein level