Dinámica familiar ante la revelación de la orientación homosexual de los hijos/as

Esta investigación tuvo por objeto averiguar cómo se ve influida la dinámica familiar cuando los hijos/as revelan su orientación homosexual. Para ello, se realizó una entrevista semiestructurada a padres y madres cuyos hijos/as son gays y/o lesbianas. Esta entrevista contenía una serie de preguntas relacionadas con la reacción y actuación en el seno familiar ante la manifestación de la homosexualidad de sus hijos. Los resultados apuntan que cada familia procede ante la homosexualidad de sus hijos de forma diversa, si bien es cierto que coinciden en pensamientos, sentimientos y actuaciones. La característica más destacada es que en todas las familias, al principio se reacciona de forma negativa y se manifiesta un rechazo abierto y un ocultamiento de esta realidad. Con el paso del tiempo, se da una acomodación y las relaciones familiares mejoran, aunque los padres siguen teniendo dificultades para integrar y aceptar con normalidad la nueva situación y continúan ocultando socialmente la orientación sexual de sus hijos.This research was to find out how it looks influenced family dynamics when the children reveal their homosexual orientation. To do so, did a semi-structured interview to parents whose children are gay or lesbian. This interview contained a series of questions related to the reaction and action within the family before the manifestation of homosexuality from their children. The results suggest that each one of the families comes to homosexuality from their children in a diverse way, while it is true that coincide in thoughts, feelings and actions. The most striking feature is that in all families, initially will react negatively and manifests an open rejection and concealment of this fact. With the passage of time the situation and family relations improve although parents continue to have difficulties to integrate and accept as normal and continue hiding socially the sexual orientation of their children

Controle da podridão cinzenta da maçã por produtos naturais biologicamente ativos

Indexación: Web of Science; ScieloBiorend SC (chitosan), BC-1000 EC (grapefruit extract plus bioflavonoids) and ECO-100 SC (bioflavonoids plus organic acids, citric phytoalexins, fatty acids, glycerides and sugars), respectively, suppressed grey rot of apple caused by B. cinerea by 80.1%, 79.0% and 76.5% when used as post-harvest treatments under controlled conditions. When applied as combined pre- and post-harvest treatments Biorend SC inhibited fruit rot by 49.9 %, while BC-1000 EC and ECO-100 SC were ineffective. None of the products inhibited fruit rot when applied as pre-harvest treatments under controlled conditions or as post-harvest treatments under commercial conditions. The algal polysaccharide ulvan used in post-harvest treatments suppressed grey rot by 56.0% under controlled conditions, but had no inhibitory effect on combined pre- and post-harvest treatments. The inability of products to activate defense mechanisms (chitinase and peroxidase) of fruits was consistent with the unsuccessful control of rot by pre-harvest treatment. The results suggest that the natural products used have potential for use in integrated management of Botrytis rot when applied after harvest.Biorend SC (quitosana), BC-1000 EC (extrato de toranja mais flavonóides), e ECO-100 SC (bioflavonóides mais ácidos orgânicos, fitoalexinas cítricas, ácidos graxos glicerídeos e açúcares) inibiram em 80,1%, 79,0% e 76,5%, respectivamente, a podridão causada por Botrytis cinerea quando utilizados no tratamento pós-colheita de frutos de maçã sob condições controladas.Tratamento combinado de Biorend SC, com aplicação tanto em pré como no pós-colheita, proporcionou 49,9% de inibição da podridão, enquanto BC-1000 e ECO-100 EC não foram efetivos. Nenhum desses produtos inibiu a podridão cinzenta, quando utilizados em tratamento de pré-colheita em condições controladas ou em tratamento de pós-colheita em condições comerciais. O polissacarídeo algal ulvana, utilizado nos tratamentos de pós-colheita, reduziu em 56% a podridão cinzenta das maçãs em condições controladas, mas não teve efeito inibitório nos tratamentos combinados de pré e pós-colheita. A incapacidade dos produtos em ativar mecanismos de defesa (quitinases e peroxidases) nos frutos, após o tratamento em pré-colheita, foi consistente com a falta de controle da podridão nesse tipo de ensaio. Pelos resultados, sugere-se que os produtos naturais utilizados apresentam potencial para a utilização no manejo integrado da podridão de Botrytis quando aplicados em pós-colheita.http://ref.scielo.org/kcxb9

Involvement of etfA gene during CaCO3 precipitation in Bacillus subtilis biofilm

The eftA gene in Bacillus subtilis has been suggested to be involved in the oxidation/reduction reactions during fatty acid metabolism. Interestingly etfA deletion in B. subtilis results in impairment in CaCO3 precipitation on the biofilm. Comparisons between the wild type B. subtilis 168 and its etfA mutant during in vitro CaCO3 crystal precipitation (calcite) revealed changes in phospholipids membrane composition with accumulation of up to 10% of anteiso-C17:0 and 11% iso-C17:0 long fatty acids. Ca2+ nucleation sites such as dipicolinic acid and teichoic acids seem to contribute to the CaCO3 precipitation. etfA mutant strain showed up to 40% less dipicolinic acid accumulation compared with B. subtilis 168, while a B. subtilis mutant impaired in teichoic acids synthesis was unable to precipitate CaCO3. In addition, B. subtilis etfA mutant exhibited acidity production leading to atypical flagella formation and inducing extensive lateral growth on the biofilm when grown on 1.4% agar. From the ecological point of view, this study shows a number of physiological aspects that are involved in CaCO3 organomineralization on biofilms

Dispersal of human and plant pathogens biofilms via nitric oxide donors at 4°C

Recent studies suggest that nitric oxide donors capable of manipulating nitric oxide-mediated signaling in bacteria could induce dispersal of biofilms. Encased in extracellular polymeric substances, human and plant pathogens within biofilms are significantly more resistant to sanitizers. This is particularly a problem in refrigerated environments where food is processed. In an exercise aimed to study the potential of nitric oxide donors as biofilm dispersal in refrigerated conditions, we compared the ability of different nitric oxide donors (SNAP, NO-aspirin and Noc-5) to dislodge biofilms formed by foodborne, human and plant pathogens treated at 4 °C. The donors SNAP and Noc-5 were efficient in dispersing biofilms formed by Salmonella enterica, pathogenic Escherichia coli and Listeria innocua. The biomasses were decreased up to 30 % when compared with the untreated controls. When the plant pathogens Pectobacterium sp. and Xanthomonas sp. were tested the dispersion was mainly limited to Pectobacterium carotovorum biofilms, decreasing up to 15 % after exposure to molsidomine. Finally, the association of selected nitric oxide donors with sanitizers (DiQuat, H2O2, peracetic acid and PhenoTek II) was effective in dispersing biofilms. The best dispersal was achieved by pre-treating P. carotovorum with molsidomine and then peracetic acid. The synergistic effect was estimated up to ~35 % in dispersal when compared with peracetic acid alone. The association of nitric oxide donors with sanitizers could provide a foundation for an improved sanitization procedure for cleaning refrigerate environments

Viscothermal Losses in Double-Negative Acoustic Metamaterials

[EN] The influence of losses in double-negative metamaterial slabs recently introduced by Graciá-Salgado et al. [Phys. Rev. B 88, 224305 (2013)] is comprehensively studied. Viscous and thermal losses are considered in the linearized Navier-Stokes equations with no flow. Despite the extremely low thicknesses of boundary layers associated with each type of losses, the double-negative behavior is totally suppressed for the rigid structures under analysis. In other words, almost 100% of the energy transmitted into the slab is dissipated by viscothermal effects, in agreement with experimental data. Simulations undertaken for larger structures, using scale factors of up to 20 times, show that double-negative behavior is never recovered. The huge dissipation obtained by these structures leads us to propose them as interesting alternatives to conventional absorbers for specific situations, e.g., when treating low frequencies or when the excitation is narrow banded.V. M. G.-C. and J. S.-D. acknowledge the support from the Spanish Ministerio de Economia y Competitividad (MINECO), and the European Union Fondo Europeo de Desarrollo Regional (FEDER) through Project No. TEC 2014-53088-C3-1-R.Cutanda-Henriquez, V.; Garcia Chocano, VM.; Sánchez-Dehesa Moreno-Cid, J. (2017). Viscothermal Losses in Double-Negative Acoustic Metamaterials. Physical Review Applied. 8(1):014029-1-014029-12. doi:10.1103/PhysRevApplied.8.014029S014029-1014029-128

Some paradigms for visualizing parallel execution of logic programs

This paper addresses the design of visual paradigms for observing the parallel execution of logic programs. First, an intuitive method is proposed for arriving at the design of a paradigm and its implementation as a tool for a given model of parallelism. This method is based on stepwise reñnement starting from the deñnition of basic notions such as events and observables and some precedence relationships among events which hold for the given model of parallelism. The method is then applied to several types of parallel execution models for logic programs (Orparallelism, Determinate Dependent And parallelism, Restricted and-parallelism) for which visualization paradigms are designed. Finally, VisAndOr, a tool which implements all of these paradigms is presented, together with a discussion of its usefulness through examples

Neuronal Density in Primary Visual Cortex (17 Visual Area), in Two Species of Octodon

Indexación: ScieloEstudios experimentales demuestran que modificaciones medioambientales pueden producir alteraciones en el desarrollo normal de la corteza cerebral visual y sus conexiones. Por otra parte, es posible que en condiciones naturales, las especies animales hayan desarrollado adaptaciones genéticas a las distintas condiciones de luminosidad en que realizan su actividad. Recientemente, se han observado variaciones significativas en la densidad neuronal cortical del área 17 (área visual primaria), en roedores silvestres con diferentes períodos diarios de actividad y relación filogenética distante (Abrothrix olivaceus y Phyllotis darwini), pero aún no se ha determinado la naturaleza genética o plástica de dichas diferencias. En este trabajo se compararon especies con una mayor cercanía filogenética, para disminuir al máximo la variable taxonómica. Se estudió la corteza visual primaria (área 17), de roedores silvestres nativos, de las especies Octodon degus (n=5) y Octodon bridgesi (n=3), pertenecientes a la Familia Octodontidae, con el propósito de evidenciar cambios a través de la medición de la densidad neuronal, mediante la técnica del disector óptico, en cortes de 40 µm, incluidos en celoidina y teñidos con Nissl. Complementariamente, se realizó una cuantificación de la densidad neuronal de la corteza motora de las especies en estudio. O. degus, que presenta un período de actividad diurna, evidenció una densidad neuronal menor en la corteza visual (34,32 ± 2,51 x 104 neuronas/mm3), que la observada en O. bridgesi (39,55 ± 0,64 x 104 neuronas/mm3), especie de período de actividad nocturna; lo cual fue estadísticamente significativo (t=3,44; p<0,05). Las diferencias encontradas se podrían relacionar con el tipo de condiciones de luminosidad en que se desenvuelven dichas especies, aunque no se puede descartar la influencia de otros factores. SUMMARY: Studies show that environmental modifications can produce profound alterations in the normal development of the visual cortex and its connectivity. For the other hand it is possible that in natural conditions, animal species have developed genetic adaptations to the different conditions of luminance in which they normally behave. Recently have observed significant changes in cortical neuronal density of area 17 (primary visual area), in two sympatric Chilean rodents with different daily activity (Phyllotis darwini and Abrothrix olivaceus), but have not yet determined the genetic nature or plastic such differences. In this paper we compared species with a closer phylogenetic relation so as to minimize the taxonomic variable. We studied the primary visual cortex (area 17) of wild rodents native of the species Octodon degus (n=5) and Octodon bridgesi (n=3), belonging to the Octodontidae family, in order to show changes in the neuronal density, using celloidin-embedded, 40µm-thickness Nissl sections, with the aid of an optical dissector. In addition, we performed a quantification of the neuronal density of the motor cortex of the species under study. O. degus, bearing a crepuscular-diurnal activity pattern, showed a lower neuronal density in the visual cortex (34.32 ± 2.51 x104 neuron/mm3) than that observed in O. bridgesi (39.55 ± 0.64 x104 neuron/mm3), a species that exhibits a nocturnal phase preference, which was statistically significant (t=3.44; p<0.05). These differences might be related to differences in daily activity in two species, but we cannot discount the influence of other factors

SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000

In recent years, multidrug resistance of Shigella strains associated with genetic elements like pathogenicity islands, have become a public health problem. The Shigella resistance locus pathogenicity island (SRL PAI) of S. flexneri 2a harbors a 16Kbp region that contributes to the multidrug resistance phenotype. However, there is not much information about other functions such as metabolic, physiologic or ecological ones. For that, wild type S. flexneri YSH6000 strain, and its spontaneous SRL PAI mutant, 1363, were used to study the contribution of the island in different growth conditions. Interestingly, when both strains were compared by the Phenotype Microarrays, the ability to metabolize D-aspartic acid as a carbon source was detected in the wild type strain but not in the mutant. When D-aspartate was added to minimal medium with other carbon sources such as mannose or mannitol, the SRL PAI-positive strain was able to metabolize it, while the SRL PAI-negative strain did not. In order to identify the genetic elements responsible for this phenotype, a bioinformatic analysis was performed and two genes belonging to SRL PAI were found: orf8, coding for a putative aspartate racemase, and orf9, coding for a transporter. Thus, it was possible to measure, by an indirect analysis of racemization activity in minimal medium supplemented only with D-aspartate, that YSH6000 strain was able to transform the D-form into L-, while the mutant was impaired to do it. When the orf8-orf9 region from SRL island was transformed into S. flexneri and S. sonnei SRL PAI-negative strains, the phenotype was restored. Also, when single genes were cloned into plasmids, no complementation was observed. Our results strongly suggest that the aspartate racemase and the transporter encoded in the SRL pathogenicity island are important for bacterial survival in environments rich in D-aspartate

Conocimiento previo en física de estudiantes de ingeniería

Se presentan resultados de un estudio de conocimiento previo en física, con estudiantes de primer año de ingeniería de la Universidad de La Frontera, Chile, en el marco de la teoría de campos conceptuales de Vergnaud, desarrollada para el estudio de aprendizaje de conceptos científicos. El propósito del estudio fue determinar niveles de comprensión de conceptos de física que describan la estructura del conocimiento previo de los estudiantes en un curso inicial de física, a partir del uso de los conceptos a) En situaciones de contexto cotidiano, b) En la comprensión de un texto de divulgación científica, y c) En situaciones y problemas de contexto académico. Los resultados obtenidos confirman bajos niveles de compresión de los estudiantes al enfrentar situaciones y lecturas que demandan una conceptualización científica aceptable. Se observa un predominio de aspectos procedimentales de las operaciones y escaso uso de significados de mayor riqueza conceptual.Some research results of a study on previous knowledge in Physics, carried out with first year engineering students at the University of The Frontera, Chile are presented. Vergnaud's Conceptual Fields Theory is used to discover the way to learn scientific concepts. The purpose of the study was to determine comprehension levels of concepts of physics, which describe the structure of the previous knowledge students had in an initial course of Physics, from the use of concepts: a) In situations of daily context, b) In the comprehension of a text of scientific dissemination, and c) In situations and problems of academic context. The obtained results confirm low levels of understanding of the students when facing situations and readings which require a scientific acceptable conceptualization. A predominance of procedural aspects of the operations and limited use of meanings of major conceptual richness is observed