Selection for Self-Fertility and Self-Fertility in Alfalfa as a Tool for Breeding Strategy Assessment

Cultivar development in alfalfa relies on heterosis, that has been mainly exploited by intercrossing selected clones from diverse gemplasm sources; an alternative way was proposed, based on developing double cross cultivars obtained by crossing clones selected for self-sterility, propagated by somatic seed. A different breeding strategy was recently emphasized based on population improvement through inbreeding and recurrent selection; in this case, self-sterility would be selected against. If self-sterility is determined by genetic load, the second strategy should be preferred, and selecting for self-fertility may bring about positive correlated responses for forage yield. Divergent selection for self-fertility was applied in a central Italian alfalfa landrace to test this hypothesis. Ten self-fertile and ten self-sterile plants were selected and hand-crossed without emasculation in a n(n-1) diallel; seeds of reciprocal crosses were pooled, obtaining 45 full-sib families per fertility group. Ten plants per family were evaluated for self-fertility. Selection was effective for self-fertility (h2 R=0.52), but not for self-sterility, a result expected if self-sterility is determined by genetic load. The forage yield of self-fertile progenies was 135% of the unselected control and 123% of the self-sterile progenies in a dense stand trial (first cut, seedling year), indicating that selection for self-fertility could be a tool for population improvement

Spitzer-MIPS survey of the young stellar content in the Vela Molecular Cloud-D

A new, unbiased Spitzer-MIPS imaging survey (~1.8 square degs) of the young stellar content of the Vela Molecular Cloud-D is presented. The survey is complete down to 5mJy and 250mJy at 24micron (mu) and 70mu, respectively. 849 sources are detected at 24mu and 52 of them also have a 70mu counterpart. The VMR-D region is one that we have already partially mapped in dust and gas millimeter emission, and we discuss the correlation between the Spitzer compact sources and the mm contours. About half of the 24mu sources are located inside the region delimited by the 12CO(1-0) contours (corresponding to only one third of the full area mapped with MIPS) with a consequent density increase of about 100% of the 24mu sources [four times for 70mu ones] moving from outside to inside the CO contours. About 400 sources have a 2MASS counterpart. So we have constructed a Ks vs. Ks-[24] diagram and identified the protostellar population. We find an excess of Class I sources in VMR-D in comparison with other star forming regions. This result is reasonably biased by the sensitivity limits, or, alternatively, may reflect a very short lifetime (<=10^6yr) of the protostellar content in this cloud. The MIPS images have identified embedded cool objects in most of the previously identified starless cores; in addition, there are 6 very young, possibly Class 0 objects identified. Finally we report finding of the driving sources for a set of five out of six very compact protostellar jets previously discovered in near-infrared images.Comment: 29 pages, 14 figures. To appear in Ap.

Pre-Main Sequence variables in the VMR-D : identification of T Tauri-like accreting protostars through Spitzer-IRAC variability

We present a study of the infrared variability of young stellar objects by means of two Spitzer-IRAC images of the Vela Molecular Cloud D (VMR-D) obtained in observations separated in time by about six months. By using the same space-born IR instrumentation, this study eliminates all the unwanted effects usually unavoidable when comparing catalogs obtained from different instruments. The VMR-D map covers about 1.5 square deg. of a site where star formation is actively ongoing. We are interested in accreting pre-main sequence variables whose luminosity variations are due to intermittent events of disk accretion (i.e. active T Tauri stars and EXor type objects). The variable objects have been selected from a catalog of more than 170,000 sources detected at a S/N ratio > 5. We searched the sample of variables for ones whose photometric properties are close to those of known EXor's. These latter are monitored in a more systematic way than T Tauri stars and the mechanisms that regulate the observed phenomenology are exactly the same. Hence the modalities of the EXor behavior is adopted as driving criterium for selecting variables in general. We selected 19 bona fide candidates that constitute a well-defined sample of new variable targets for further investigation. Out of these, 10 sources present a Spitzer MIPS 24 micron counterpart, and have been classified as 3 Class I, 5 flat spectrum and 2 Class II objects, while the other 9 sources have spectral energy distribution compatible with phases older than Class I. This is consistent with what is known about the small sample of known EXor's, and suggests that the accretion flaring or EXor stage might come as a Class I/II transition. We present also new prescriptions that can be useful in future searches for accretion variables in large IR databases.Comment: 35 pages, 12 figures To appear in Ap

Near- and Far-Infrared Counterparts of Millimeter Dust Cores in the Vela Molecular Ridge Cloud D

The aim of this paper is to identify the young protostellar counterparts associated to dust millimeter cores of the Vela Molecular Ridge Cloud D through new IR observations (H_2 narrow-band at 2.12 micron and N broad band at 10.4 micron) along with an investigation performed on the existing IR catalogues. The association of mm continuum emission with infrared sources from catalogues (IRAS, MSX, 2MASS), JHK data from the literature and new observations, has been established according to spatial coincidence, infrared colours and spectral energy distributions. Only 7 out of 29 resolved mm cores (and 16 out of the 26 unresolved ones) do not exhibit signposts of star formation activity. The other ones are clearly associated with: far-IR sources, H_2 jets or near-IR objects showing a high intrinsic colour excess. The distribution of the spectral indices pertaining to the associated sources is peaked at values typical of Class I objects, while three objects are signalled as candidates Class 0 sources. We remark the high detection rate (30%) of H_2 jets driven by sources located inside the mm-cores. They appear not driven by the most luminous objects in the field, but rather by less luminous objects in young clusters, testifying the co-existence of both low- and intermediate-mass star formation. The presented results reliably describe the young population of VMR-D. However, the statistical evaluation of activity vs inactivity of the investigated cores, even in good agreement with results found for other star forming regions, seems to reflect the limiting sensitivity of the available facilities rather than any property intrinsic to the mm-condensations.Comment: 38 pages. To be published to Astronomy & Astrophysic

ISO observations of M8, the Lagoon nebula

In this paper, IRAS, ISO, and molecular line observations of the M8 and M8E sources in the Lagoon Nebula are reported

phytotherapeutic compound yhk exerts an inhibitory effect on early stage of experimentally induced neoplastic liver lesions

The aim of this study was to investigate the effects of the herbal compound YHK on hepatocarcinogenesis induced by diethylntrosamine (DEN) in Sprague Dawley rats. Rats were randomly divided into 3 groups and followed up for 15 weeks. Groups 1 was given standard food and represented the healthy control. Liver preneoplastic foci were induced using the DEN method in groups 2 and 3 (20 rats each). However, group 3 was concomitantly given 50mg/kg/day of YHK. For quantitative assessment of liver preneoplastic foci, the placental form of glutathione-S-transferase (GST-P) positive foci were measured using immunohistochemical staining and image analysis. Treatment using DEN caused a significant decrease in body weight and increase in liver weight compared to the control group while concomitant supplementation with YHK prevented body weight loss and liver weight increase. As compared to DENonly treated rats, the group given YHK showed a significant decrease in the number, size and volume of GSTP-positive foci. Moreover, co-administration of YHK significantly reduced the incidence, number, size and volume of hepatocellular carcinoma. Anti-inflammatory, anti-fibrotic as well as antioxidative properties of this compound are mechanisms which are likely to be advocated for to explain its protective effect. It is concluded that herbal compound YHK by preventing hepatocarcinogenesis in DEN-induced liver preneoplastic lesions in rats has the potential to a large clinical application as a functional food

Spitzer-IRAC survey of molecular jets in Vela-D

We present a survey of H2 jets from young protostars in the Vela-D molecular cloud (VMR-D), based on Spitzer -IRAC data between 3.6 and 8.0 micron. Our search has led to the identification of 15 jets and about 70 well aligned knots within 1.2 squared degree. We compare the IRAC maps with observations of the H2 1-0 S(1) line at 2.12 micron, with a Spitzer-MIPS map at 24 and 70 micron, and with a map of the dust continuum emission at 1.2 mm. We find a association between molecular jets and dust peaks. The jet candidate exciting sources have been searched for in the published catalog of the Young Stellar Objects of VMR-D. We selected all the sources of Class II or earlier which are located close to the jet center and aligned with it.The association between jet and exciting source was validated by estimating the differential extinction between the jet opposite lobes. We are able to find a best-candidate exciting source in all but two jets. Four exciting sources are not (or very barely) observed at wavelengths shorter than 24 micron, suggesting they are very young protostars. Three of them are also associated with the most compact jets. The exciting source Spectral Energy Distributions have been modeled by means of the photometric data between 1.2 micron and 1.2 mm. From SEDs fits we derive the main source parameters, which indicate that most of them are low-mass protostars. A significant correlation is found between the projected jet length and the [24] - [70] color, which is consistent with an evolutionary scenario according to which shorter jets are associated with younger sources. A rough correlation is found between IRAC line cooling and exciting source bolometric luminosity, in agreement with the previous literature. The emerging trend suggests that mass loss and mass accretion are tightly related phenomena and that both decrease with time.Comment: Accepted by The Astrophysical Journa

The Spitzer-IRAC Point Source Catalog of the Vela-D Cloud

This paper presents the observations of the Cloud D in the Vela Molecular Ridge, obtained with the IRAC camera onboard the Spitzer Space Telescope at the wavelengths \lambda = 3.6, 4.5, 5.8, 8.0 {\mu}m. A photometric catalog of point sources, covering a field of approximately 1.2 square degrees, has been extracted and complemented with additional available observational data in the millimeter region. Previous observations of the same region, obtained with the Spitzer MIPS camera in the photometric bands at 24 {\mu}m and 70 {\mu}m, have also been reconsidered to allow an estimate of the spectral slope of the sources in a wider spectral range. A total of 170,299 point sources, detected at the 5-sigma sensitivity level in at least one of the IRAC bands, have been reported in the catalog. There were 8796 sources for which good quality photometry was obtained in all four IRAC bands. For this sample, a preliminary characterization of the young stellar population based on the determination of spectral slope is discussed; combining this with diagnostics in the color-magnitude and color-color diagrams, the relative population of young stellar objects in the different evolutionary classes has been estimated and a total of 637 candidate YSOs have been selected. The main differences in their relative abundances have been highlighted and a brief account for their spatial distribution is given. The star formation rate has been also estimated and compared with the values derived for other star forming regions. Finally, an analysis of the spatial distribution of the sources by means of the two-point correlation function shows that the younger population, constituted by the Class I and flat-spectrum sources, is significantly more clustered than the Class II and III sources.Comment: Accepted by Ap