Role of non-coding RNAs in non-aging-related neurological disorders

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOProtein coding sequences represent only 2% of the human genome. Recent advances have demonstrated that a significant portion of the genome is actively transcribed as non-coding RNA molecules. These non-coding RNAs are emerging as key players in the regula518CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOsem informaçãosem informação2016/22447-52011/506802013/07559-

Lipase And Esterase - To What Extent Can This Classification Be Applied Accurately? [lipases E Esterases: Como Definir E Classificar?]

Enzyme technology is an ever-growing field of knowledge and, in recent years, this technology has raised renewed interest, due to the search for new paradigms in several productive processes. Lipases, esterases and cutinases are enzymes used in a wide range of processes involving synthesis and hydrolysis reactions. The objective of this work was to investigate and compare the specific lipase and esterase activities of five enzymes - four already classified as lipases and one classified as cutinase - in the presence of natural and synthetic substrates. All tested enzymes presented both esterase and lipase specific activities. The highest specific esterase activity was observed for Aspergillus 1068 lipase in natural substrate and for F. oxysporum cutinase in synthetic substrate, while the highest specific lipase activity was observed for Geotrichum sp. lipase in natural substrate and for F. oxysporum cutinase in synthetic substrate. These results display some interface-independent lipolytic activity for all lipases tested. 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(Eds.), Braunschweig: GBF MonographsEttinger, W.F., Thukral, S.K., Kolattukudy, P.E., Structure of cutinase gene, cDNA, and the derived amino acid sequence from phytopathogenic fungi (1987) Biochemistry, 26, pp. 7883-7892. , http://dx.doi.org/10.1021/bi00398a052Ghamgui, H., Karra-Chaâbouni, M., Gargouri, Y., 1-Butyl oleate synthesis by immobilized lipase from n-hexane and solvent-free system (2004) Enzyme and Microbial Technology, 35 (4), pp. 355-363. , http://dx.doi.org/10.1016/j.enzmictec.2004.06.002Helistö, P., Korpela, T., Effects of detergents on activity of microbial lipases as measured by the paranitrophenyl alkanoate esters method (1998) Enzyme and Microbial Technology, 23 (1-2), pp. 113-117. , http://dx.doi.org/10.1016/S0141-0229(98)00024-6Holmquist, M., Alpha Beta-Hydrolase Fold Enzymes Structures, Functions and Mechanisms (2000) Current Protein and Peptide Science, 1 (2), pp. 209-235. , http://dx.doi.org/10.2174/1389203003381405, PMid:12369917(2007) Esters Database, , www.ensam.inra.fr/, INLAND NORTHWEST RESEARCH ALLIANCE-INRA, Montpellier: INRA. Disponível emKim, G.S., Screening, production and properties of a stereospecific esterase from Pseudomonas sp. S34 with high selectivity to (S)-ketoprofen ethyl ester (2002) Journal of Molecular Catalysis B: Enzymatic, 17 (1), pp. 29-38. , http://dx.doi.org/10.1016/S1381-1177(01)00077-7Kolattukudy, P.E., Cutinases from fungi and pollen (1984) Lipases, pp. 471-504. , BORGSTROM, B.BROCKMAN, H. (Eds.), Amsterdam: ElsevierKulkarni, N., Gadre, R.V., Production and properties of alkaline, thermophilic lipase from Pseudomonas fluorenscens NS2W (2002) Journal of Industrial Microbiology and Biotechnology, 28 (6), pp. 344-348. , http://dx.doi.org/10.1038/sj.jim.7000254, PMid:12032808Labuschagne, R.B., van Tonder, A., Litthauer, D., Flavobacterium odoratum lipase: Isolation and characterization (1997) Enzyme Microbial Technology, 21 (1), pp. 52-58http://dx.doi.org/10.1016/S0141-0229(96)00226-8Lin, Y.C., Wu, J.Y., Chen, T.L., Production of Acinetobacter radioresistens lipase with repeated batch culture in presence of nonwoven fabric (2001) Biotechnology and Bioengineering, 76 (3), pp. 214-218. , http://dx.doi.org/10.1002/bit.1185, PMid:11668456Longhi, S., Cambillau, C., Structure-activity of cutinase, a small lipolytic enzyme (1999) Biochimica Et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1441 (2-3), pp. 185-196Macedo, G.A., Produção, purificação, caracterização bioquímica e aplicações de lipase de Geotrichum sp (1995) Dissertação, 121, p. 1995. , (Mestrado em Tecnologia dos Alimentos)-Universidade Estadual de Campinas, CampinasMacedo, G.A., Park, Y.K., Pastore, G.M., Partial purification and characterization of an extracellular lipase from a newly isolated strain of Geotrichum sp (1997) Brazilian Journal of Microbiology, 39, pp. 687-692Macedo, G.A., Pio, T.F., A rapid screening method for cutinase producing microorganisms (2005) Brazilian Journal of Microbiology, 36 (4), pp. 388-394. , http://dx.doi.org/10.1590/S1517-83822005000400016Mahadik, N.D., (2002) Production of Acidic Lipase By Aspergillus Niger In Solid State Fermentation. 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Estimating additive and dominance variances for complex traits in pigs combining genomic and pedigree information

Knowledge of dominance effects should improve ge-netic evaluations, provide the accurate selection of purebred animals, and enable better breeding strategies, including the exploitation of het-erosis in crossbreeds. In this study, we combined genomic and pedi-gree data to study the relative importance of additive and dominance genetic variation in growth and carcass traits in an F2 pig population. Two GBLUP models were used, a model without a polygenic effect (ADM) and a model with a polygenic effect (ADMP). Additive effects played a greater role in the control of growth and carcass traits than did dominance effects. However, dominance effects were important for all traits, particularly in backfat thickness. The narrow-sense and broad-sense heritability estimates for growth (0.06 to 0.42, and 0.10 to 0.51, respectively) and carcass traits (0.07 to 0.37, and 0.10 to 0.76, respec-tively) exhibited a wide variation. The inclusion of a polygenic effect in the ADMP model changed the broad-sense heritability estimates only for birth weight and weight at 21 days of age

One Europe, one neurologist?

Eur J Neurol. 2007 Mar;14(3):241-7. One Europe, one neurologist? Grisold W, Galvin R, Lisnic V, Lopes Lima J, Mueller E, Oberndorfer S, Vodusek DB; UEMS-EBN and EFNS Education Committee. Department of Neurology and Ludwig Boltzmann Institute for Neurooncology , Kaiser Franz Josef Hospital of the City of Vienna, Vienna, Austria. [email protected] Abstract In recent years, there has been a major shift in emphasis within neurology from being a largely diagnostic discipline to one much more actively involved in treating disease. There have been major scientific advances leading to new and effective treatments. There is also a much greater awareness of the burden of neurological disease (Olesen J, Leonardi M. European Journal of Neurology 2003; 10: 471) and informed sufferers are requesting specific intervention. There is wide variation in the delivery of neurological services throughout Europe. This is reflected in manpower levels, the place of neurology related to other medical specialties and different mixes of hospital and private office practice. These differences have been thrown into sharper focus by the recent expansion of the European Union (EU). Initial training in neurology is given to undergraduate/pre-graduate students. Post-graduate education is delivered within a residency program leading to specialist qualification and certification. We now recognize that this is only the beginning of a life long program of continuous education and development (CME/CPD). National and international exchange programs facilitate the growth of knowledge and promote professional harmony and cooperation. The free migration of medical specialists has been an aspiration but remains limited by cultural, linguistic, personal, professional, political and economic factors. Two bodies, the European Board of Neurology (EBN-UEMS) http://www.uems-neuroboard.org (Union Européenne des Médecins Spécialistes) and the European Federation of Neurological Societies (EFNS) http://www.efns.org are actively involved in harmonising and developing neurology at the European level. PMID: 17355542 [PubMed - indexed for MEDLIN

Electron impact ionization of 1-propanol

Experimental measurements of the cations created in electron impact ionization have been undertaken for the primary isomer of propanol using a Hidden Quadrupole Mass Spectrometer (EPIC 300), with a mass resolution of 1 amu. The mass spectra recorded at an incident electron energy of 70 eV reveals the relative probability of forming 50 different cations, by either direct ionization or dissociative ionization. Individual partial ionization cross sections (PICS) for 31 different cations were measured for the first time in this work, for the incident electron energy range from 10 to 100eV. Also, appearance energies (AEs) and Wannier exponents for the 16 most intense cations formed in electron collisions with 1-propanol are reported. Where possible, those results are compared to those from an earlier investigation. Total Ionization Cross Sections (TICS) were also obtained from the sum of the measured PICS, for nearly all cations measured, and are compared to relevant data reported in the literature. In addition, as a part of this study, theoretical TICS were calculated using the Binary-encounter Bethe (BEB) and independent atom model with screening corrected additivity rule (IAM - SCAR) methods. Good agreement between current measured and calculated TICSs and corresponding earlier results was typically found

Intermediate-energy differential and integral cross sections for vibrational excitation in α-tetrahydrofurfuryl alcohol

9 pags.; 5 figs.; 6 tabs.Differential and integral cross section measurements, for incident electron energies in the 20-50 eV range, are reported for excitation of several composite vibrational modes in α-tetrahydrofurfuryl alcohol (THFA). Optimisation and frequency calculations, using GAUSSIAN 09 at the B3LYP/aug-cc-pVDZ level, were also undertaken for the two most abundant conformers of THFA, with results being reported for their respective mode classifications and excitation energies. Those calculations assisted us in the experimental assignments of the composite features observed in our measured energy loss spectra. There are, to the best of our knowledge, no other experimental or theoretical data currently available in the literature against which we can compare the present results. © 2014 AIP Publishing LLC.Spanish (Ministerio de Economia y Competitividad under Project FIS2012-31230) funding agencies who financially supported various aspects of this work.Peer Reviewe

Novel Perspectives in Management of Angiogenesis and Cancer Therapy

Funding: The project was funded by IPOLFG EPE and by iNOVA4Health (UID/Multi/04462/2019) a program financially supported by Fundação para a Ciência e Tecnologia (FCT)/Ministério da Educação e Ciência, through national funds. We also acknowledge funding from FCT-MCTES through the project DREAM—PTDC/MEC-ONC/29327/2017. FL-C PhD fellowship was funded by FCT (PD/BD/128337/2017).The activation of endothelial cells (ECs) is a crucial step on the road map of tumor angiogenesis and expanding evidence indicates that a pro-oxidant tumor microenvironment, conditioned by cancer metabolic rewiring, is a relevant controller of this process. Herein, we investigated the contribution of oxidative stress-induced ferroptosis to ECs activation. Moreover, we also addressed the anti-angiogenic effect of Propranolol. We observed that a ferroptosis-like mechanism, induced by xCT inhibition with Erastin, at a non-lethal level, promoted features of ECs activation, such as proliferation, migration and vessel-like structures formation, concomitantly with the depletion of reduced glutathione (GSH) and increased levels of oxidative stress and lipid peroxides. Additionally, this ferroptosis-like mechanism promoted vascular endothelial cadherin (VE-cadherin) junctional gaps and potentiated cancer cell adhesion to ECs and transendothelial migration. Propranolol was able to revert Erastin-dependent activation of ECs and increased levels of hydrogen sulfide (H2S) underlie the mechanism of action of Propranolol. Furthermore, we tested a dual-effect therapy by promoting ECs stability with Propranolol and boosting oxidative stress to induce cancer cell death with a nanoformulation comprising selenium-containing chrysin (SeChry) encapsulated in a fourth generation polyurea dendrimer (SeChry@PUREG4). Our data showed that novel developments in cancer treatment may rely on multi-targeting strategies focusing on nanoformulations for a safer induction of cancer cell death, taking advantage of tumor vasculature stabilization.publishersversionpublishe