Emociones y psicoterapia: caminos e intersecciones

En este libro, dirigido a profesionales y estudiantes del campo de la psicología, se reúnen diversas investigaciones centradas en las emociones desde las dimensiones psicológica, social y cultural y despliega diversas formas de trabajo, regulación, expresión y reconfiguración emocional en aras de un mayor bienestar psicológico. Como parte del alivio psicológico, las emociones son condición que favorece el trabajo psicoterapéutico para generar condiciones de bienestar en las personas y afrontar lo emocional desde la psicoterapia, remite al tema de la pertenencia y la identidad; por tanto, la comprensión del vínculo inquebrantable entre el cuerpo y las emociones resulta fundamental para su gestión.ITESO. A.C

The Structural Diversity of Carbohydrate Antigens of Selected Gram-Negative Marine Bacteria

Marine microorganisms have evolved for millions of years to survive in the environments characterized by one or more extreme physical or chemical parameters, e.g., high pressure, low temperature or high salinity. Marine bacteria have the ability to produce a range of biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents, and as a result, they have been a topic of research interest for many years. Among these biologically active molecules, the carbohydrate antigens, lipopolysaccharides (LPSs, O-antigens) found in cell walls of Gram-negative marine bacteria, show great potential as candidates in the development of drugs to prevent septic shock due to their low virulence. The structural diversity of LPSs is thought to be a reflection of the ability for these bacteria to adapt to an array of habitats, protecting the cell from being compromised by exposure to harsh environmental stress factors. Over the last few years, the variety of structures of core oligosaccharides and O-specific polysaccharides from LPSs of marine microrganisms has been discovered. In this review, we discuss the most recently encountered structures that have been identified from bacteria belonging to the genera Aeromonas, Alteromonas, Idiomarina, Microbulbifer, Pseudoalteromonas, Plesiomonas and Shewanella of the Gammaproteobacteria phylum; Sulfitobacter and Loktanella of the Alphaproteobactera phylum and to the genera Arenibacter, Cellulophaga, Chryseobacterium, Flavobacterium, Flexibacter of the Cytophaga-Flavobacterium-Bacteroides phylum. Particular attention is paid to the particular chemical features of the LPSs, such as the monosaccharide type, non-sugar substituents and phosphate groups, together with some of the typifying traits of LPSs obtained from marine bacteria. A possible correlation is then made between such features and the environmental adaptations undertaken by marine bacteria

Oral abstracts of the 21st International AIDS Conference 18-22 July 2016, Durban, South Africa

The rate at which HIV-1 infected individuals progress to AIDS is highly variable and impacted by T cell immunity. CD8 T cell inhibitory molecules are up-regulated in HIV-1 infection and associate with immune dysfunction. We evaluated participants (n=122) recruited to the SPARTAC randomised clinical trial to determine whether CD8 T cell exhaustion markers PD-1, Lag-3 and Tim-3 were associated with immune activation and disease progression.Expression of PD-1, Tim-3, Lag-3 and CD38 on CD8 T cells from the closest pre-therapy time-point to seroconversion was measured by flow cytometry, and correlated with surrogate markers of HIV-1 disease (HIV-1 plasma viral load (pVL) and CD4 T cell count) and the trial endpoint (time to CD4 count <350 cells/μl or initiation of antiretroviral therapy). To explore the functional significance of these markers, co-expression of Eomes, T-bet and CD39 was assessed.Expression of PD-1 on CD8 and CD38 CD8 T cells correlated with pVL and CD4 count at baseline, and predicted time to the trial endpoint. Lag-3 expression was associated with pVL but not CD4 count. For all exhaustion markers, expression of CD38 on CD8 T cells increased the strength of associations. In Cox models, progression to the trial endpoint was most marked for PD-1/CD38 co-expressing cells, with evidence for a stronger effect within 12 weeks from confirmed diagnosis of PHI. The effect of PD-1 and Lag-3 expression on CD8 T cells retained statistical significance in Cox proportional hazards models including antiretroviral therapy and CD4 count, but not pVL as co-variants.Expression of ‘exhaustion’ or ‘immune checkpoint’ markers in early HIV-1 infection is associated with clinical progression and is impacted by immune activation and the duration of infection. New markers to identify exhausted T cells and novel interventions to reverse exhaustion may inform the development of novel immunotherapeutic approaches

Learning to represent exact numbers

This article focuses on how young children acquire concepts for exact, cardinal numbers (e.g., three, seven, two hundred, etc.). I believe that exact numbers are a conceptual structure that was invented by people, and that most children acquire gradually, over a period of months or years during early childhood. This article reviews studies that explore children’s number knowledge at various points during this acquisition process. Most of these studies were done in my own lab, and assume the theoretical framework proposed by Carey (2009). In this framework, the counting list (‘one,’ ‘two,’ ‘three,’ etc.) and the counting routine (i.e., reciting the list and pointing to objects, one at a time) form a placeholder structure. Over time, the placeholder structure is gradually filled in with meaning to become a conceptual structure that allows the child to represent exact numbers (e.g., There are 24 children in my class, so I need to bring 24 cupcakes for the party.) A number system is a socially shared, structured set of symbols that pose a learning challenge for children. But once children have acquired a number system, it allows them to represent information (i.e., large, exact cardinal values) that they had no way of representing before

Abstract B47: Neural cells labeling with intracerebral ferumoxytol iron oxide nanoparticles in a rat model.

Abstract Purpose: To investigate uptake of ferumoxytol iron oxide nanoparticles into rat neural cells for a clinically applicable magnetic resonance (MR) imaging technique. Materials and Methods: Long Evan rats were injected with intracerebral ferumoxytol (360 μg) iron oxide nanoparticles into the right hemisphere. A needle track in the left hemisphere was used as a sham control. Animals underwent T2-weighted fast spin-echo MRI sequences at 12T 48 h after nanoparticle inoculation. Immediately after MRI, single brain cell suspensions were produced from the right and left hemispheres, and neurospheres were stained for iron using Prussian blue histochemstry. Results: The ferumoxytol-injected right hemisphere showed decreased T2 signal compared to the sham injected left hemisphere. Positive iron staining was found in neural cells from the right cerebral hemisphere, although &amp;lt;5% of total cell number, while no positive iron staining cells were found in the left hemisphere. The decreasing T2 signal does not correlate the percentage of neural cells labeled with ferumoxytol iron oxide nanoparticles, indicating most of the nanoparticles were not cell-associated. The phenotypes of ferumoxytol-labeled neural cells will be characterized by immunohistochemistry for neural markers. No neural cell in vitro labeling was detected using ferumoxytol in combination with protamine sulfate and heparin. Conclusion: Iron oxide loading of neural cells in the brain parenchyma can be achieved with the clinically approved agent ferumoxytol. Iron labeling may be utilized to monitor neuro-inflammation and brain tumors after chemotherapy for non-invasive MRI. Citation Format: Yingjen Jeffrey Wu, Leslie L. Muldoon, Edward A. Neuwelt. Neural cells labeling with intracerebral ferumoxytol iron oxide nanoparticles in a rat model. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology: Multidisciplinary Science Driving Basic and Clinical Advances; Dec 2-5, 2012; Miami, FL. Philadelphia (PA): AACR; Cancer Res 2013;73(1 Suppl):Abstract nr B47.</jats:p

Abstract 4477: Safety and efficacy of intracarotid administration of temozolomide in a rat model of intracerebral metastasis.

Abstract Purpose: Temozolomide (Temodar; Merck) is an alkylating chemotherapeutic agent used to treat glioblastoma and other brain malignancies. After oral administration, the average brain interstitium to plasma area under the drug concentration-time curve ratio for temozolomide has been reported as approximately 0.2 in animal models and patients. We hypothesized that intra-arterial (IA) drug administration into the internal carotid artery would improve temozolomide delivery to brain tissue, with subsequent increased anti-tumor efficacy. Methods: Athymic nude rats received intracerebral implantation of LX-1 human small cell lung carcinoma cells as a model of chemosensitive brain metastasis, and were studied 10 days after cell implantation when tumors measured 10-30 mm3 in volume. To assess drug delivery, rats received 25 μCi 14C-temozolomide by oral, intravenous (IV), or IA/osmotic blood-brain barrier disruption (BBBD) route of administration (n = 5-7 per group). Radiolabeled drug in tumor and brain was measured 10 min after drug administration by quantitative autoradiography. To assess efficacy, rats received either no treatment or a single treatment with 20 mg/kg temozolomide (∼150 mg/m2) by oral, IV, or IA route of administration (n = 8 per group) and rats were followed for survival. Results: The ratio of drug delivery in tumor compared to normal left hemisphere was equivalent for the oral (2.51 ± 25) and IV (2.56 ± 0.87) routes (P = 0.90). After IA/BBBD, the tumor to brain ratio was 4.11 ± 1.12 and tumor-specific 14C-radioactivity was 85.4 ± 29.9 nCi/gram tissue, compared to 38.1 ± 19.9 nCi/gram tissue after IV administration (P = 0.019 by Student t-test). Median survival in untreated control rats was 17.5 days. A single temozolomide dose was effective at increasing survival when given by oral (median survival 25.5 days), IV (25.5 days), or IA (33 days) route of administration (overall P&amp;lt;0.0001 by the Log Rank test). Survival times in the oral and IV groups were significantly longer than control (P&amp;lt;0.001) but did not differ from each other, while survival time in the IA group was significantly longer than all other groups (P&amp;lt;0.01 for all comparisons). Temozolomide could not be given with BBBD in the efficacy study due to toxicities likely related to the diluent; there was no evidence of neurotoxicity in rats given IA temozolomide. Conclusions: Infusion of temozolomide into the internal carotid artery improved drug delivery and safely increased efficacy in a rat intracerebral tumor model. The improved efficacy suggests that a clinical trial of IA temozolomide is warranted in refractory glioblastoma. Citation Format: Leslie L. Muldoon, Michael A. Pagel, Edward A. Neuwelt. Safety and efficacy of intracarotid administration of temozolomide in a rat model of intracerebral metastasis. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4477. doi:10.1158/1538-7445.AM2013-4477</jats:p

Inhibition of SUR1 Decreases the Vascular Permeability of Cerebral Metastases

AbstractInhibition of sulfonylurea receptor 1 (SUR1) by glyburide has been shown to decrease edema after subarachnoid hemorrhage. We investigated if inhibiting SUR1 reduces cerebral edema due to metastases, the most common brain tumor, and explored the putative association of SUR1 and the endothelial tight junction protein, zona occludens-1 (ZO-1). Nude rats were intracerebrally implanted with small cell lung carcinoma (SCLC) LX1 or A2058 melanoma cells (n = 36). Rats were administered vehicle, glyburide (4.8 µg twice, orally), or dexamethasone (0.35 mg, intravenous). Blood-tumor barrier (BTB) permeability (Ktrans) was evaluated before and after treatment using dynamic contrast-enhanced magnetic resonance imaging. SUR1 and ZO-1 expression was evaluated using immunofluorescence and Western blots. In both models, SUR1 expression was significantly increased (P < .05) in tumors. In animals with SCLC, control mean Ktrans (percent change ± standard error) was 101.8 ± 36.6%, and both glyburide (-21.4 ± 14.2%, P < .01) and dexamethasone (-14.2 ± 13.1%, P < .01) decreased BTB permeability. In animals with melanoma, compared to controls (117.1 ± 43.4%), glyburide lowered BTB permeability increase (3.2 ± 15.4%, P < .05), while dexamethasone modestly lowered BTB permeability increase (63.1 ± 22.1%, P > .05). Both glyburide (P < .001) and dexamethasone (P < .01) decreased ZO-1 gap formation. By decreasing ZO-1 gaps, glyburide was at least as effective as dexamethasone at halting increased BTB permeability caused by SCLC and melanoma. Glyburide is a safe, inexpensive, and efficacious alternative to dexamethasone for the treatment of cerebral metastasis-related vasogenic edema

Improved Perfusion MR Imaging Assessment of Intracerebral Tumor Blood Volume and Antiangiogenic Therapy Efficacy in a Rat Model with Ferumoxytol

PURPOSE: To evaluate the consistency of tumor blood volume measurements and antiangiogenic therapy efficacy assessments with a low-molecular-weight gadolinium-based contrast agent (GBCA, gadodiamide) versus an iron oxide nanoparticle (ferumoxytol) in the presence or absence of a loading dose of contrast agent before perfusion magnetic resonance (MR) imaging (preload method). MATERIALS AND METHODS: The protocol was approved by the institutional animal care and use committee. U87MG tumor cells were implanted intracerebrally in 13 rats. All 13 rats underwent 11.75-T MR imaging with gadodiamide (60 μL) 13 days after tumor implantation. The next day, nine rats underwent MR imaging with ferumoxytol (60 μL). Immediately after ferumoxytol imaging, six rats received bevacizumab (45 mg/kg). MR imaging was repeated 48 hours after bevacizumab treatment with gadodiamide and 72 hours after treatment with ferumoxytol. Each study included three consecutive dynamic susceptibility-weighted contrast material–enhanced (DSC) MR acquisitions, which were performed without preload, with single-dose preload, and with double-dose preload. Tumor relative cerebral blood volume (rCBV) was estimated from each DSC MR acquisition. Two-way repeated measures analysis of variance was performed to test for differences between groups with both contrast agents. RESULTS: DSC MR imaging with gadodiamide and without preload showed low rCBV (≤1.75) in nine of the 13 tumors; estimated rCBV increased progressively with both single- and double-dose preloads (P < .001). Conversely, rCBVs obtained with ferumoxytol were high (>1.75) and remained constant with all three acquisitions. The magnitude of rCBV decrease after bevacizumab administration was dependent on the dose of gadodiamide preload, whereas the magnitude of rCBV decrease with ferumoxytol was constant regardless of whether contrast agent preload was used. CONCLUSION: With GBCA, tumor rCBV can be underestimated without preload and becomes dose dependent with preload correction. Conversely, ferumoxytol provides consistent assessment of tumor rCBV and antiangiogenic therapy efficacy. © RSNA, 201