Evaluation de la pratique de l'autogreffe de cellules souches hématopoiétiques dans le service d'hématologie de l'hôpital Nord

AIX-MARSEILLE2-BU Pharmacie (130552105) / SudocSudocFranceF

Insecticides cytotoxicity and CYP1A1 induction in primary human and rat hepatocytes cultures

With the increasing demand for insecticide products, the question of their safety has become one of the serious world public health concerns. The capability of compounds belonging to the major insecticide families [such as chlorinated hydrocarbons (DDT), carbamates (carbaryl: CBR), organophosphorus compounds (malathion, tetrachlorvinfos: MAL, TCV), pyrethroids (cypermethrin: CPR) and benzoylurea (diflubenzuron: DFU)] in inducing CYP1 Al in rat and human hepatocyte cultures has been tested. Cells were treated during 3 days with six non-toxic increasing doses of insecticides and CYP1A1 expression was assessed by ethoxyresorufin O-deethylase (EROD) activity and by Northern blots. A strong and dose-dependent induction was observed with TCV and DFU, both in human (approx. five- and sevenfold over control, respectively) and in rat hepatocytes (approx. sevenfold). However, EROD induction and CYP/A1 mRNA levels were correlated for DFU but not for TCV, suggesting different regulation mechanisms for PCYP1A1 gene expression by the two compounds. CBR and CPR exerted less induction in both cell types (approx. 2.5-fold induction compared with approximately 16-fold for 3-methylcholanthrene), whereas DDT and MAL showed no action on human hepatocytes but decreased EROD activity in rat cells. Finally, cytotoxicity studies performed using the MTT and the neutral red tests demonstrated significant differences between insecticides

Insecticide cytotoxicity and CYP1A1/2 induction in primary human and rat hepatocyte cultures

International audienc

Comparative study of CYP1A1 induction by 3-methylcholanthrene in various human hepatic and epidermal cell types

International audienc

Positive feedback between future climate change and the carbon cycle

International audienceFuture climate change due to increased atmospheric CO2 may affect land and ocean efficiency to absorb atmospheric CO2. Here, using climate and carbon three-dimensional models forced by a 1% per year increase in atmospheric CO•, we show that there is a positive feedback between the climate system and the carbon cycle. Climate change reduces land and ocean uptake of CO•, respectively by 54% and 35% at 4 x CO•. This negative impact implies that for prescribed anthropogenic COy. emissions, the atmospheric CO• would be higher than the level reached if climate change does not affect the carbon cycle. We estimate the gain of this climate-carbon cycle feedback to be 10% at 2 x CO2 and 20% at 4 x CO•. This translates into a 15% higher mean temperature increase

La Bretagne collectionne l'art de notre temps : Les vingt ans du FRAC Bretagne

This catalogue documents six exhibitions organized in the summer of 2001 to celebrate the 20th anniversary of the Brittany FRAC. The curators of six institutions affiliated with the FRAC elaborate on the development of their collection through themes that best represent them (space, landscape, history…). President of FRAC P. Le Treut states how the Brittany region, which holds collections including works by nearly 140 artists, is open to current art produced elsewhere while sharing its specific heritage. List of works. Texts in French with English translation booklet. 17 bibl. ref

Non-Small Cell Lung Cancer Patients Harboring HER2 Mutations: Clinical Characteristics and Management in a Real-Life Setting. Cohort HER2 EXPLORE GFPC 02–14

Background: Mutation of human receptor tyrosine kinase epidermal growth factor receptor-2 (HER2) is a rare event, found in approximately 1% non-small cell lung cancers (NSCLC). The objective was to investigate the clinical characteristics and management of HER2-mutated NSCLCs in a real-life setting. Methods: This multicenter study described NSCLCs harboring HER2 mutations diagnosed between January 2012 and December 2014, according their clinical characteristics, management, and outcomes: response rate (RR), progression-free survival (PFS), and overall survival (OS). Results: Thirty patients were included: 66.7% women; median age 65.2 ± 12 years; never or former smokers 73.3%. Of the stage IV patients (n = 23), 86% received first-line platin doublet chemotherapy: RR 61.5% and PFS 6.7 (95% CI 5.9-9.5) months; 52.1% received a second-line therapy: RR 18.2% and PFS 4.9 (95% CI 2.5-11.9) months. Median OS of stage IV was 10.7 months and 2-year OS was 27.2% (95% CI 11.7-63.2). All patients with stage I-III NSCLCs were alive at 2 years. Conclusion: The rarity of HER2-mutated NSCLCs requires specific studies. Enhanced digital features To view enhanced digital features for this article go to https://doi.org/10.6084/ m9.figshare.8138837