Correction of destructive changes in connective tissues of different organs during chronic nitrate and fluoride intoxication by nanosized silica oxide

Akimov O. Ye., Kovalova I. O., Kostenko V. O. Correction of destructive changes in connective tissues of different organs during chronic nitrate and fluoride intoxication by nanosized silica oxide. Journal of Education, Health and Sport. 2019;9(5):547-555. eISNN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.3238594 http://ojs.ukw.edu.pl/index.php/johs/article/view/6997 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26/01/2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Authors 2019; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 29.04.2019. Revised: 15.05.2019. Accepted: 24.05.2019. "XVIII V. V. Podvysotskiy Readings" May, 21-22, Odessa Materials of conference CORRECTION OF DESTRUCTIVE CHANGES IN CONNECTIVE TISSUES OF DIFFERENT ORGANS DURING CHRONIC NITRATE AND FLUORIDE INTOXICATION BY NANOSIZED SILICA OXIDE O. Ye. Akimov, I. O. Kovalova, V. O. Kostenko Ukrainian Medical Stomatological Academy, Poltava, Ukraine Abstract This experiment carried on 35 white Wistar rats aimed at studying the markers of connective tissue destruction during chronic nitrate-fluoride intoxication and its correction by nanosized silica oxide. It has been found out that co-administration of sodium fluoride (10 mg/kg body weight) and sodium nitrate (500 mg/kg of body weight) for 30 days increases the total content of glycosaminoglycans (GAG), heparin/heparan and keratan/dermatan fractions, as well as content of chondroitin fraction and concentration of L-oxyproline in the gastric mucosa. The content of N-acetylneuraminic acid (NANA), free L-oxyproline and gexuronic acids are increased in femoral bone and lumbar vertebrae. These changes point to depolymerization of componets of organic connective tissues matrix (collagen, glycoproteins, proteoglycans). Application of nanosized silica oxide suspension under conditions of chronic nitrate-fluoride intoxication reduces the total GAG content, keratan / dermatane fraction ratio, chondroitin fraction and L-oxyproline concentration in the gastric mucosa. We also observed a decrease in free L-oxyproline concentration, gexuronic acids and NANA content in femoral bone and vertebrae. The results allowed us to conclude that chronic nitrate-fluoride intoxication leads to the destruction of connective tissue components in different organs (gastric mucous membrane, femoral bones and vertebrae). Usage of nanosized silica oxide under conditions of chronic nitrate-fluoride intoxication reduces the destruction of connective tissue in these structures. Key words: nitrates, fluorides, nanosized silica oxide, chronic intoxication, gastric mucosa, bone

Основні напрямки удосконалення діяльності служби дільничних інспекторів міліції щодо попередження насильства в сім’ї

Ковальова, О. В. Основні напрямки удосконалення діяльності служби дільничних інспекторів міліції щодо попередження насильства в сім’ї / О. В. Ковальова // Вісник Харківського національного університету внутрішніх справ. - 2007. – Вип. 38. - С. 206-215Розглянуто основні напрямки удосконалення діяльності служби дільничних інспекторів міліції щодо попередження насильства в сім’ї. Підкреслено, що дії різних органів, установ та організацій, уповноважених здійснювати попередження насильства в сім’ї, доволі часто не скоординовані, спрямовуються переважно на реалізацію специфічних, суто відомчих завдань, що не дає відчутних результатів. Рассмотрены основные направления совершенствования деятельности службы участковых инспекторов милиции по предупреждению насилия в семье. Подчеркнуто, что действия различных органов, учреждений и организаций, уполномоченных осуществлять предупреждении насилия в семье, зачастую не скоординированы, направляются преимущественно на реализацию специфических, сугубо ведомственных задач, не дает ощутимых результатов. The main directions of improving the activities of the service of district police inspectors for the prevention of domestic violence are considered. It is emphasized that the actions of various bodies, institutions and organizations authorized to prevent domestic violence are often not coordinated, they are aimed primarily at the implementation of specific, purely departmental tasks, and do not produce tangible results

Determination of the chromosome aberrations frequency and the dynamics of acrocentric chromosomes associations in children with type I diabetes

Abstract To date, it’s known that in Ukraine there are about 1 million patients with diabetes, but their real number is 2–3 times more, since this disease is usually diagnosed at the stage of already manifested pathological process. Unfortunately, diabetes mellitus remains an incurable disease, but it is possible to prolong life of patients or improve its quality by preventing or delaying its complications. Objective. To study quantitative and qualitative cytogenetic indices in peripheral blood lymphocytes of children with type 1 diabetes mellitus. Materials and methods. Cytogenetic study was performed in 30 children with type 1 diabetes aged from 5 to 17 years and 40 practically healthy peers. Associations of acrocentric chromosomes (AAX) were evaluated using criteria developed by K. D. Zang and E. Back (1968). Metaphase plate analysis was performed using a Leica Galen III binocular microscope (Austria), an eyepiece of ×15, a lens of ×100, a binocular nozzle of ×1.25. The statistical processing of the results was carried out using Excel application packages, SPSS Statistica17.0. Results: Changes in the somatic cells genome stability of children with type 1 diabetes have been revealed. Thus, the spontaneous level of chromosomal aberrations in the main and control groups was within 3.66 % and 0.96 %, respectively, and significantly exceeded the control indices (P < 0.005). In children, genomic abnormalities in the form of polyploidy and hypoploidy, as well as a decrease in CL0+2, were found, which indicates a decrease in the immunoreactivity of the sick children organism and allows to consider them as persons with secondary immunodeficiency. Conclusions: The spontaneous level of chromosomal aberrations in peripheral blood lymphocytes in children with type 1 diabetes significantly exceeded the control indices. Cytogenetic examination of children with type 1 diabetes has revealed genomic disorders in the form of polyploid cells, which have not been detected in the control group. Changes in the genome stability in the form of hypoploid aneuploidy have also been found in the examined group. In children with type 1 diabetes a decrease in the acrocentric chromosomes ability to join associations has been identified, which makes it possible to consider these children as persons with secondary immunodeficiency

Особистісні фактори психологічного благополуччя в умовах соціально-економічної нестабільності

The relevance of the topic is in the peculiarities of the formation of psychological well-being under the influence of destructive phenomena of the socioeconomic environment. The modern geopolitical space and economic trends encourage the solution of problems not only at the macroeconomic level and stabilization of the economy but also at the level of the individual, which is the prerogative of the state social policy. The factors of psychological well-being formation may differ in their nature and direction, which necessitates a methodical analysis of the available factors. The issue of using pedagogical, spiritual, and cultural practices to ensure the formation of psychological well-being is a key task in the implementation of the mechanism for creating a positive psychological climate. The psychological climate in which a person lives is an important factor in the development of psychological well-being. The purpose of the article is to study the personal factors of psychological well-being in the context of socioeconomic instability and global geopolitical challenges. The task of the research is to outline the key prospects for the development of psychological well-being and its possible formation following the conditions of socioeconomic space. The research methodology involves the implementation of a series of scientific research methods, as well as the use of correlation analysis to identify the main advantages of psychological well-being in conditions of socioeconomic instability. The results obtained can improve social policy and psychological practices of assisting people with low levels of psychological well-being. The practical significance of the article lies in the possibility of using the obtained results to improve the individual development of personal factors of psychological well-being. In the context of global geopolitical challenges, it is worth directing human cognitive activity to the analysis of the elimination of destructive phenomena in psychological well-being. The article investigates the peculiarities of the development along with the formation of characteristic features of psychological well-being and identifies the key directions of development of practices for their improvement and optimization.Актуальність теми полягає в особливостях формування психологічного благополуччя під впливом деструктивних явищ соціально-економічного середовища. Сучасний геополітичний простір та економічні тенденції спонукають до вирішення проблем не тільки на макроекономічному рівні і стабілізації економіки, а й на рівні особи, яка є прерогативою державної соціальної політики. Фактори психологічного благополуччя формування можуть відрізнятися за своїм характером і спрямованістю, що зумовлює необхідність аналізу наявних факторів. Питання використання педагогічних, духовних, культурних практик забезпечення формування психологічного благополуччя є ключовим завданням в реалізації механізму створення позитивного психологічного клімату. Психологічний клімат, в якому живе людина, є важливим фактором розвитку психологічного благополуччя. Метою статті є вивчення особистісних чинників психологічного благополуччя в контексті соціоекономічної нестабільності і глобальних геополітичних викликів. Завдання дослідження окреслити ключові перспективи розвитку психологічного благополуччя та його можливі формування відповідно до умов соціально-економічного простору. Методологія дослідження передбачає використання кореляційного аналізу для виявлення основних переваг психологічного благополуччя в умовах соціально-економічної нестабільності. Отримані результати можуть удосконалити соціальну політику та психологічні практики допомоги людям з низьким рівнем психологічного благополуччя. Практичне значення статті полягає в можливості використання отриманих результатів для вдосконалення індивідуального розвитку особисті фактори психологічного благополуччя. У контексті глобальних геополітичних викликів, варто спрямувати пізнавальну діяльність людини на аналіз, усунення деструктивних явищ у психологічному самопочутті. Стаття досліджує особливості розвитку разом із формуванням характерних рис психологічного благополуччя та визначає практики їх удосконалення та оптимізації

Therapeutic strategy in patients with long-lasting essential hypertension with comorbid type 2 diabetes mellitus and obesity

Background: Presently the level of blood pressure (BP) control is extremely unsatisfactory in hypertensive patients throughout the world. The aim of our study was to find the optimal drug therapy for patients with hard-to-treat essential hypertension (EH) associated with type 2 diabetes mellitus (T2DM) and obesity, namely the comparison of strategies of fixed and non-fixed combination. Material and methods: Eighty-seven patients with EH, T2DM and obesity were enrolled into the study. Two groups were formed: the 1st group — 41 patients received antihypertensive therapy in the form of unfixed combination of drugs (“multi-pill”) perindopril, indapamide and amlodipine; the 2nd — 46 patients, who received the same drugs, but in a fixed-dose combination (“single pill”). Results: A favorable treatment result was found for fixed-dose combination of antihypertensive drugs, with significant reduction in the frequency of visits to the doctor: relative risk (RR) — 1.27 (95% CI: 1.01‒1.61), p = 0.045, and odds ratio (OR) — 3.10 (95% CI: 1.05‒9.13), p = 0.04. That indicates that patients on fixed-dose combination were less likely to visit a doctor with complaints. Patients on single-pill therapy were less likely to get to progression (worsening) group in contrast to multi-pill non-fixed combination: RR — 1.37 (95% CI: 1.02‒1.84), p = 0.03; OR — 2.91 (95% CI: 1.12‒7.59), p = 0.03. Conclusion: The single-pill triple combination has significant advantage compared to multi-pill regimen in hard-totreat hypertensive patients with comorbid T2DM and obesity. Fixed-dose triple combination leads to significantly faster achievement of blood pressure control

The use of augmented reality technologies in the development of emotional intelligence of future specialists of socionomic professions under the conditions of adaptive learning.

In modern conditions, innovative augmented reality technologies are actively developing, which are widespread in many areas of human activity. Intro-duction of advanced developments in the process of professional training of future specialists of socionomic professions in the conditions of adaptive training, contributes to the implementation of the principles of a personalized approach and increase the overall level of competitiveness. The relevant sci-entific article is devoted to the theoretical and empirical analysis of the fea-tures of the implementation of augmented reality technologies in the con-struct of traditional psychological and pedagogical support aimed at the de-velopment of emotional intelligence of the future specialist. The interdisci-plinary approach was used while carrying out the researchwork at the ex-pense of the general fund of the state budget: "Adaptive system for individu-alization and personalization of professional training of future specialists in the conditions of blended learning". A comprehensive study of the imple-mentation of traditional psychological-pedagogical and innovative augment-ed reality technologies was conducted in the framework of scientific cooper-ation of STEAM-Laboratory, Laboratory of Psychophysiological Research and Laboratory of Psychology of Health in Bogdan Khmelnytsky Melitopol State Pedagogical University. The theoretical analysis considers the structural model of emotional intelligence of the future specialist of socionomic pro-fessions, which is represented by two structural components: intrapersonal construct of emotional intelligence (understanding and analysis of emotions; use of emotions to improve thinking and activity) and interpersonal construct of emotional intelligence (perception, evaluation and expression). emotions; conscious management of emotions). Each component mediates the inherent emotional intelligence of interpretive, regulatory, adaptive, stress-protective and activating functions. The algorithm of the empirical block of research is presented by two stages: ascertaining and forming research. According to the results of the statement, low indicators were found on most scales, reflecting the general level of emotional intelligence development of future specialists, actualizing the need to find and implement effective measures for the devel-opment of emotional intelligence components in modern higher education and taking into account information development and digitalization. As part of the formative stage of the research implementation, a comprehensive pro-gram "Development of emotional intelligence of future professionals" was tested, which integrated traditional psychological and pedagogical technolo-gies and innovative augmented reality technologies. This program is designed for 24 hours, 6 thematic classes of 4 hours. According to the results of a comprehensive ascertaining and shaping research, the effectiveness of the influence of augmented reality technologies on the general index of emo-tional intelligence is proved. The stepby- step model of integration of aug-mented reality components influencing the ability to analyze, understand and regulate emotional states into a complex program of emotional intelligence development is demonstrated. According to the results of the formative study, there is a dominance of high indicators of the following components: intrapersonal (50%), interpersonal (53.3%). Thus, we can say that in-trapersonal and interpersonal emotional intelligence together involve the ac-tualization of various cognitive processes and skills, and are related to each other. Empirical data were obtained as a result of conducting a psychodiag-nostic study on an innovative computer complex -psychotest

Effect of antitumor drugs in low concentrations on the biological, immunophenotypic and cytogenetic characteristics of human colon cancer cells in vitro

Objective: To estimate the impact of the low-dose anticancer drugs (ACD) with the different mechanisms of action and human interferon (IFN) alpha 2b on the biological properties, immunophenotypic and cytogenetic characteristics of colon cancer cells in vitro. Materials and Methods: The study was performed on human colon cancer cell lines COLO 205, HT-29 and 3C-P treated with ACD and IFN in subtoxic concentrations. Expression of CD44, N-cadherin, vimentin, β-catenin, ERCC1 and Slug was assessed by immunocytochemical method. Using cytogenetic analysis, the numbers of mitoses, cells with micronuclei, apoptotic cells and cells with nuclear protrusions were studied. Results: The prolonged exposure (up to 30 days) of colon cancer cells to low-dose ACD (0.2–0.5 µg/ml cisplatin and 0.1–0.2 µg/ml irinotecan) in combination with IFN (500–1000 IU/ml) led to 37-fold decreased colony-forming activity of these cell and 10-fold reduction of the number of cells expressing mesenchymal protein markers (N-cadherin, vimentin). Also, in COLO 205 cells treated with ACD and IFN the number of SLUG- and CD44-positive cells decreased by 92 and by 85%, respectively. Long-term cultivation of HT-29 cells in the presence of cisplatin and IFN resulted in 5-fold suppression of ERCC1 expression. The cytogenetic analysis has shown that the ACD, IFN and their combinations in subtoxic concentrations caused significant genotoxic effect, suppression of cell proliferation and accumulation of cells with micronuclei. The sensitivity of colon cancer cells to ACD in standard cytotoxic concentrations did not change after prolonged low-dose exposure. Conclusion: The data showed that the prolonged action of the low doses of ACD on human colon cancer cells resulted in the suppression of cell proliferation, colony-forming activity in soft agar, expression of epithelialmesenchymal transition-associated markers and significant cytogenetic changes

Подбор имплантатов для замены слуховых косточек человека на основе модели связанных контуров

У роботі, на основі моделі зв’язаних контурів, вирішується актуальна у пластичній хірургії ЛОР-органів проблема індивідуального підбору матеріалу для заміщення слухових кісточок середнього вуха людини.In this work, based on the model of coupled circuits, decided actual plastic surgery ENT problem of individual selection of material to replace the auditory ossicles of the middle ear of man.В работе, на основе модели связанных контуров, решается актуальная в пластической хирургии ЛОР-органов проблема индивидуального подбора материала для замещения слуховых косточек среднего уха человека

Features of implementation of modern AR technologies in the process of psychological and pedagogical support of children with autism spectrum disorders

The article deals with the actual issue of the specificity and algorithm of the introduction of innovative AR technologies in the process of psychological and pedagogical support of children with autism spectrum disorders (ASD). An innovative element of theoretical and methodological analysis of the problem and empirical research is the detection of vectors of a constructive combination of traditional psycho-correctional and psycho-diagnostic approaches with modern AR technologies. The analysis of publications on the role and possibilities of using AR technologies in the process of support children with ASD (autism spectrum disorder) and inclusive environment was generally conducted by surfing on the Internet platforms containing the theoretical bases for data publications of scientific journals and patents. The article also analyzes the priorities and potential outcomes of using AR technologies in psycho-correction and educational work with autistic children. According to the results of the analysis of scientific researches, Unified clinical protocol of primary, secondary (specialized), tertiary (highly specialized) medical care and medical rehabilitation “Autism spectrum disorders (disorders of general development)”, approaches for correction, development and education of children with ASD, AR technologies were selected for further implementation in a comprehensive program of psychological and pedagogical support for children with ASD. The purpose of the empirical study is the search, analysis and implementation of multifunctional AR technologies in the psycho-correctional construct of psychological and pedagogical support of children with ASD. According to the results of the pilot study, the priorities and effectiveness of using AR technologies in the development of communicative, cognitive, emotional-volitional, mnemonic abilities of children and actualization of adaptive potential and adaptive, socially accepted behaviors are made. The possibilities and perspectives of using AR technologies as an element of inclusive environment, with regard to nosology and phenomenology, need further investigation

Основные методы детекции инцидентов с безопасностью пациентов в медицине

Цель исследования. Изучить таксономию понятия «инцидент с безопасностью пациента», предоставить современную классификацию и сравнительную характеристику существующих методов обнаружения таких инцидентов в медицине. Материалы и методы. Контент-анализ специальной медицинской литературы и материалов электронных интернет-ресурсов на предмет современных технологий и инструментов идентификации проблем с безопасностью пациентов, их классификации и ключевых характеристик. Результаты. Показано, что весь спектр возможных инцидентов, связанных с безопасностью пациентов, разделяется в две категории: медицинская ошибка и неблагоприятное событие. Раскрыта и детализирована таксономия каждого из этих понятий. Выбраны 4 критерия для стратификации всех существующих методов идентификации инцидентов, связанных с безопасностью пациентов, на группы. Представлена характеристика основных. Заключение. Универсального метода детекции инцидентов, связанных с безопасностью пациентов, не существует. Только комплексный подход к проблеме идентификации неблагоприятных событий в медицине может обеспечить получение целостной картины; Objective. The aim of the research is to explore the taxonomy of the concept of «patient safety incident», to provide a modern classification and comparative characteristics of existing methods of incidents detection in medicine. Materials and methods. Content analysis of special medical literature and electronic Internet resources on the subject of modern technologies and tools for identifying problems with patient safety, their classification and key characteristics. Results. It has been shown that the full spectrum of possible incidents with patient safety falls into two categories – a medical error and an adverse event. The taxonomy of each of them is disclosed and detailed. Four criteria for stratifying all existing methods of patient safety incidents identification are chosen. The characteristics of the main ones are given. Conclusion. There is no universal method for detecting patient safety incidents. Only an integrated approach to the problem of identifying adverse events in medicine can provide a relatively holistic picture