123 research outputs found
Powertrain Component Topology Optimisation
This paper aims to describe how a topology optimisation process has been implemented in Jaguar Land Rover’s Powertrain Research Design Team through the delivery of an example component, the cam-carrier of an in-line 6 cylinders petrol engine. It first focuses on optimisation in general, describing its base principles as well as more detailed concepts, before to explain how the optimisation has been run on the example component. Then, this paper evaluates how to create and implement a self-contained topology optimisation process within a design team, without disrupting the already existing processes.This paper aims to describe how a topology optimisation process has been implemented in Jaguar Land Rover’s Powertrain Research Design Team through the delivery of an example component, the cam-carrier of an in-line 6 cylinders petrol engine. It first focuses on optimisation in general, describing its base principles as well as more detailed concepts, before to explain how the optimisation has been run on the example component. Then, this paper evaluates how to create and implement a self-contained topology optimisation process within a design team, without disrupting the already existing processes
Single Bead Labeling Method for Combining Confocal Fluorescence On-Bead Screening and Solution Validation of Tagged One-Bead One-Compound Libraries
SummaryScreening of one-bead one-compound libraries by incubating beads with fluorescently labeled target protein requires isolation and structure elucidation of a large number of primary hit beads. However, the potency of the identified ligands is only revealed after time consuming and expensive larger scale resynthesis and testing in solution. Often, many of the resynthesized compounds turn out to be weak target binders in solution due to large differences between surface and solution binding affinities. For an industry style high-throughput screening (HTS) process a high false positive rate is detrimental. We have therefore combined single bead and single molecule/single cell techniques into an integrated HTS process in which the picomole amount of substance contained on one isolated hit bead is sufficient for quality control, structure determination, and precise affinity determination to the target protein in solution
Caching Using Software-Defined Networking in LTE Networks
Research ReportThe data consumption is increasing rapidly in mobile net- works. The cost of network infrastructure is increasing, which will lead to an ”end of profit” within next few years. Thus, mobile operators require technology that allows in- creasing the network capacity within low network costs. There- fore, using caching is the most evident solution to be used in their backhaul networks. However, the current architec- ture of LTE network does not provide sucient flexibility to place the caches in the most optimal locations. The current work on Software-Defined Networking in Evolved Packet Core virtualization has enabled us to integrate dy- namically the caching functionality in a LTE network and improve the caching system performance. In this paper, we present the solution we designed for this aim based on Soft- ware Defined Networking technology. Moreover, we developa testbed for the proof-of-concept and we present perfor- mance analysis of this solution
Synthesis of Natural and Unnatural Cyclooligomeric Depsipeptides Enabled by Flow Chemistry.
Flow chemistry has been successfully integrated into the synthesis of a series of cyclooligomeric depsipeptides of three different ring sizes including the natural products beauvericin (1 a), bassianolide (2 b) and enniatin C (1 b). A reliable flow chemistry protocol was established for the coupling and macrocyclisation to form challenging N-methylated amides. This flexible approach has allowed the rapid synthesis of both natural and unnatural depsipeptides in high yields, enabling further exploration of their promising biological activity.The authors gratefully acknowledge the EPSRC for financial support (grants EP/K009494/1 and EP/K039520/1). This is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1002/chem.20150445
Shifting Native Chemical Ligation into Reverse through N→S Acyl Transfer
Peptide thioester synthesis by N→S acyl transfer is being intensively explored by many research groups the world over. Reasons for this likely include the often straightforward method of precursor assembly using Fmoc-based chemistry and the fundamentally interesting acyl migration process. In this review we introduce recent advances in this exciting area and discuss, in more detail, our own efforts towards the synthesis of peptide thioesters through N→S acyl transfer in native peptide sequences. We have found that several peptide thioesters can be readily prepared and, what’s more, there appears to be ample opportunity for further development and discovery
Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels–Alder Reactions
Bio-ort
hogona
llabellin
gschemes
based
on inverse-elec
tron-
deman
dDiels–Ald
er (IEDDA)
cycloa
ddition
have
attracted
much
attention
in chem
ical biology
recently
.The appeal
ing
features
of this reactio
n, such
as the fast reactio
nkinetics,
fully
bio-ort
hogonal
nature
and high
selectiv
ity,have helped
chem
i-
cal biologists
gain
deeper
understandi
ng of biochemic
al pro-
cesses
at the molecular
level.Listing
the compo
nents
and dis-
cussing
the possib
ilities
andlimitations
of thesereagent
s, we
provid
earecent
snapshot
of the field
of IEDDA
-based
biomo-
lecular
manipulatio
nwith special
focus
on fluores
cent
modula-
tion
approaches
throug
hthe use of bio-orthogon
alized
build-
ing blocks.
At the end,
we discuss
challenges
that
need
to be
addres
sed
for further
develop
ments
in order
to overcome
recent
limita
tions
and to enabl
eresearchers
to answer
biomo
-
lecular
quest
ions in more
detail
- …