Powertrain Component Topology Optimisation

This paper aims to describe how a topology optimisation process has been implemented in Jaguar Land Rover’s Powertrain Research Design Team through the delivery of an example component, the cam-carrier of an in-line 6 cylinders petrol engine. It first focuses on optimisation in general, describing its base principles as well as more detailed concepts, before to explain how the optimisation has been run on the example component. Then, this paper evaluates how to create and implement a self-contained topology optimisation process within a design team, without disrupting the already existing processes.This paper aims to describe how a topology optimisation process has been implemented in Jaguar Land Rover’s Powertrain Research Design Team through the delivery of an example component, the cam-carrier of an in-line 6 cylinders petrol engine. It first focuses on optimisation in general, describing its base principles as well as more detailed concepts, before to explain how the optimisation has been run on the example component. Then, this paper evaluates how to create and implement a self-contained topology optimisation process within a design team, without disrupting the already existing processes

Single Bead Labeling Method for Combining Confocal Fluorescence On-Bead Screening and Solution Validation of Tagged One-Bead One-Compound Libraries

SummaryScreening of one-bead one-compound libraries by incubating beads with fluorescently labeled target protein requires isolation and structure elucidation of a large number of primary hit beads. However, the potency of the identified ligands is only revealed after time consuming and expensive larger scale resynthesis and testing in solution. Often, many of the resynthesized compounds turn out to be weak target binders in solution due to large differences between surface and solution binding affinities. For an industry style high-throughput screening (HTS) process a high false positive rate is detrimental. We have therefore combined single bead and single molecule/single cell techniques into an integrated HTS process in which the picomole amount of substance contained on one isolated hit bead is sufficient for quality control, structure determination, and precise affinity determination to the target protein in solution

Caching Using Software-Defined Networking in LTE Networks

Research ReportThe data consumption is increasing rapidly in mobile net- works. The cost of network infrastructure is increasing, which will lead to an ”end of profit” within next few years. Thus, mobile operators require technology that allows in- creasing the network capacity within low network costs. There- fore, using caching is the most evident solution to be used in their backhaul networks. However, the current architec- ture of LTE network does not provide sucient flexibility to place the caches in the most optimal locations. The current work on Software-Defined Networking in Evolved Packet Core virtualization has enabled us to integrate dy- namically the caching functionality in a LTE network and improve the caching system performance. In this paper, we present the solution we designed for this aim based on Soft- ware Defined Networking technology. Moreover, we developa testbed for the proof-of-concept and we present perfor- mance analysis of this solution

Synthesis of Natural and Unnatural Cyclooligomeric Depsipeptides Enabled by Flow Chemistry.

Flow chemistry has been successfully integrated into the synthesis of a series of cyclooligomeric depsipeptides of three different ring sizes including the natural products beauvericin (1 a), bassianolide (2 b) and enniatin C (1 b). A reliable flow chemistry protocol was established for the coupling and macrocyclisation to form challenging N-methylated amides. This flexible approach has allowed the rapid synthesis of both natural and unnatural depsipeptides in high yields, enabling further exploration of their promising biological activity.The authors gratefully acknowledge the EPSRC for financial support (grants EP/K009494/1 and EP/K039520/1).  This is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1002/chem.20150445

Shifting Native Chemical Ligation into Reverse through N→S Acyl Transfer

Peptide thioester synthesis by N→S acyl transfer is being intensively explored by many research groups the world over. Reasons for this likely include the often straightforward method of precursor assembly using Fmoc-based chemistry and the fundamentally interesting acyl migration process. In this review we introduce recent advances in this exciting area and discuss, in more detail, our own efforts towards the synthesis of peptide thioesters through N→S acyl transfer in native peptide sequences. We have found that several peptide thioesters can be readily prepared and, what’s more, there appears to be ample opportunity for further development and discovery

Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels–Alder Reactions

Bio-ort hogona llabellin gschemes based on inverse-elec tron- deman dDiels–Ald er (IEDDA) cycloa ddition have attracted much attention in chem ical biology recently .The appeal ing features of this reactio n, such as the fast reactio nkinetics, fully bio-ort hogonal nature and high selectiv ity,have helped chem i- cal biologists gain deeper understandi ng of biochemic al pro- cesses at the molecular level.Listing the compo nents and dis- cussing the possib ilities andlimitations of thesereagent s, we provid earecent snapshot of the field of IEDDA -based biomo- lecular manipulatio nwith special focus on fluores cent modula- tion approaches throug hthe use of bio-orthogon alized build- ing blocks. At the end, we discuss challenges that need to be addres sed for further develop ments in order to overcome recent limita tions and to enabl eresearchers to answer biomo - lecular quest ions in more detail