Very Late Stent Thrombosis in a Drug-Eluting Stent due to Interruption of Anti-Platelet Agents in Patients With Acute Myocardial Infarction and Thrombocytosis

Stent thrombosis is a fatal complication in patients who have undergone percutaneous coronary intervention, and discontinuation of anti-platelet agent is a major risk factor of stent thrombosis. We report a rare case of very late stent thrombosis (VLST) following discontinuation of anti-platelet agents in a patient who experienced acute myocardial infarction and essential thrombocytosis. She had undergone implantation of a drug eluting stent (DES) and a bare metal stent (BMS) two and half years prior to her presentation. VLST developed in DES, not in BMS, following interruption of anti-platelet therapy

Roles of Arrest-Defective Protein 1225 and Hypoxia-Inducible Factor 1α in Tumor Growth and Metastasis

Background Vascular endothelial growth factor A (VEGFA), a critical mediator of tumor angiogenesis, is a well-characterized target of hypoxia-inducible factor 1 (HIF-1). Murine arrest-defective protein 1A (mARD1A225) acetylates HIF-1??, triggering its degradation, and thus may play a role in decreased expression of VEGFA.Methods We generated ApcMin/+/mARD1A225 transgenic mice and quantified growth of intestinal polyps. Human gastric MKN74 and murine melanoma B16F10 cells overexpressing mARD1A225 were injected into mice, and tumor growth and metastasis were measured. VEGFA expression and microvessel density in tumors were assessed using immunohistochemistry. To evaluate the role of mARD1A 225 acetylation of Lys532 in HIF-1??, we injected B16F10-mARD1A225 cell lines stably expressing mutant HIF-1??/K532R into mice and measured metastasis. All statistical tests were two-sided, and P values less than. 05 were considered statistically significant.Results ApcMin/+/mARD1A225 transgenic mice (n = 25) had statistically significantly fewer intestinal polyps than Apc Min/+ mice (n = 21) (number of intestinal polyps per mouse: Apc Min/+ mice vs ApcMin/+/mARD1A225 transgenic mice, mean = 83.4 vs 38.0 polyps, difference = 45.4 polyps, 95% confidence interval [CI] = 41.8 to 48.6; P <. 001). The growth and metastases of transplanted tumors were also statistically significantly reduced in mice injected with mARD1A225-overexpressing cells than in mice injected with control cells (P <. 01). Moreover, overexpression of mARD1A 225 decreased VEGFA expression and microvessel density in tumor xenografts (P <. 04) and ApcMin/+ intestinal polyps (P =. 001). Mutation of lysine 532 of HIF-1?? in B16F10-mARD1A225 cells prevented HIF-1?? degradation and inhibited the antimetastatic effect of mARD1A225 (P <. 001).Conclusion mARD1A225 may be a novel upstream target that blocks VEGFA expression and tumor-related angiogenesis

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

One-Pot Fabrication of Nanocomposites Composed of Carbon Nanotubes and Alumina Powder Using a Rotatable Chemical Vapor Deposition System

The fabrication of multi-dimensional nanocomposites has been extensively attempted to achieve synergistic performance through the uniform mixing of functional constituents. Herein, we report a one-pot fabrication of nanocomposites composed of carbon nanotubes (CNTs) and Al2O3 powder. Our strategy involves a synthesis of CNTs on the entire Al2O3 surface using a rotatable chemical vapor deposition system (RCVD). Ehylene and ferritin-induced nanoparticles were used as the carbon source and wet catalyst, respectively. The RCVD was composed of a quartz reaction tube, 5.08 cm in diameter and 150 cm in length, with a rotation speed controller. Ferritin dissolved in deionized water was uniformly dispersed on the Al2O3 surface and calcinated to obtain iron nanoparticles. The synthesis temperature, time, and rotation speed of the chamber were the main parameters used to investigate the growth behavior of CNTs. We found that the CNTs can be grown at least around 600 °C, and the number of tubes increases with increasing growth time. A faster rotation of the chamber allows for the uniform growth of CNT by the tip-growth mechanism. Our results are preliminary at present but show that the RCVD process is sufficient for the fabrication of powder-based nanocomposites

IMECE2002-39267 LOCALLY HEATED LOW TEMPERATURE WAFER LEVEL MEMS PACKAGING WITH CLOSED-LOOP AuSn SOLDER-LINES

ABSTRACT In this paper, locally heated closed-loop AuSn solder-line bonding method was proposed and evaluated for a lowtemperature, high strength, and hermetic MEMS packaging. We fabricated two different test specimens including substrateheated specimen and locally heated specimen in order to verify the performance of locally heated method. In air tightness test, the substrate-heated specimen and locally heated specimen show the maximum leak rate of 13.5±9.8x10 mbar-l/s with the same internal volume of 6.89±0.2x10 -6 l, respectively. In the critical pressure test, any fracture was not found in the bonded specimens at applied pressure of 10±2bar. From these results, we approximately extracted the bonding strength of the proposed bonding process of 3.53±0.07MPa. By EDS(Energy Dispersive X-ray Spectrometer) analysis at bonded interface, we found that bonded interface(between AuSn solder and Ti/Au layer) of substrate-heated specimen was stronger than that of locally heated specimen

Validation of a high performance liquid chromatography method for quantitation of foot-and-mouth disease virus antigen in vaccines and vaccine manufacturing

Foot-and-mouth disease (FMD) is an infectious viral disease that affects the main meat and dairy production animals, including cattle, sheep, goats and swine. It is readily transmissible and countries where the disease is present suffer harsh international trade restrictions on livestock products and serious economic losses. Vaccines are important tools to contain outbreaks and maintain the status of free with or without vaccination, as defined by the World Organization for Animal Health (OIE). The efficacy of vaccines is reliant on the content and integrity of inactivated virus particles. The long-established method to quantify the viral content of vaccines along the manufacturing process and in the final product is the 140S sucrose density gradient analysis. This method has been a valuable tool for many decades. However, it requires gradient preparation for each sample, a lengthy ultracentrifugation and a manual UV reading of the gradient, rendering it highly operator dependent and almost impossible to automate. We present a method to quantify FMDV particles in vaccines and intermediate process samples that is based on separation of components by size exclusion high performance liquid chromatography (SE-HPLC) and measurement of virus by absorption at 254 nm. The method has been extensively validated; it is accurate, precise and linear. It is applicable to all FMDV strains and sample materials and has a good concordance with the 140S test. The proposed method uses off the shelf HPLC equipment and columns. It is easily automated for high throughput operation, affording a useful process analytical technology and a novel tool for control of final product by manufacturers and regulatory agencies.Fil: Spitteler, Marcelo A.. Biogénesis Bagó; ArgentinaFil: Romo, Ana. Biogénesis Bagó; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Magi, Nicolás. Biogénesis Bagó; ArgentinaFil: Seo, Min Goo. Animal and Plant Quarantine Agency; Corea del SurFil: Yun, Seon-Jong. Animal and Plant Quarantine Agency; Corea del SurFil: Barroumeres, Fernando. Biogénesis Bagó; ArgentinaFil: Régulier, Emmanuel G.. Biogénesis Bagó; ArgentinaFil: Bellinzoni, Rodolfo. Biogénesis Bagó; Argentin

Prevalence of Coxiella burnetii in cattle at South Korean national breeding stock farms.

This is the first study to evaluate the serologic and molecular prevalence of Coxiella burnetii in cattle at national breeding stock farms in South Korea. These government farms have well-organized biosecurity and management systems to prevent livestock diseases. Of the 736 cattle in this study, 77 tested positive for antibodies against C. burnetii antigens (10.5%, 95% CI: 8.3-12.7) and 11 were positive for a C. burnetti infection on PCR analysis (1.5%, 95% CI: 0.6-2.4). Since the 16S rRNA sequences of C. burnetii from all 11 PCR-positive samples were identical, three representative samples (C-CN-3 from the southern region, C-JJ-9 from Jeju Island, and C-CB-37 from the central region) are described in this paper. These three sequences had 99.3-100% identity to those of C. burnetii deposited in GenBank. These sequences clustered with those from USA, Japan, and Greenland, underscoring the sequence similarity among C. burnetii isolates in these countries. Because C. burnetii was detected in cattle at well-managed national breeding stock farms, cattle at non-government operated farms may be more likely to be exposed to C. burnetii in South Korea. Thus, continuous surveillance and control strategies in animals and humans are required to prevent the transmission of C. burnetii to humans

Which Treatment is More Effective for Functional Ankle Instability: Strengthening or Combined Muscle Strengthening and Proprioceptive Exercises?

Assistimos surgir no Brasil uma nova cultura de adoção que busca famílias para crianças e não crianças para famílias. Essa proposta comporta um novo projeto de família, de maternidade e paternidade, atribuindo novos sentidos ao ser pai e mãe, distinto do tradicional modelo associado à consangüinidade. A adoção tardia insere-se nesse novo contexto. Para compreender os processos de construção de maternidade e paternidade nessas circunstâncias, é importante investigar as significações produzidas pelo casal ao tornar-se pai/mãe. Este artigo foca a produção discursiva de um casal durante entrevistas domiciliares, realizadas no decorrer do processo de adoção tardia de duas irmãs (4 e 5 anos). São apresentados os sentidos produzidos na conversação, em resposta à pergunta sobre como era ser pai ou ser mãe naquele momento. A análise aponta especificidades do tornar-se pai/mãe por adoção tardia, a difícil e frágil construção de vínculos e a necessidade de acompanhamentos pós-adotivos