41 research outputs found
Widespread phytoplankton blooms triggered by 2019–2020 Australian wildfires
Droughts and climate-change-driven warming are leading to more frequent and intense wildfires1,2,3, arguably contributing to the severe 2019–2020 Australian wildfires4. The environmental and ecological impacts of the fires include loss of habitats and the emission of substantial amounts of atmospheric aerosols5,6,7. Aerosol emissions from wildfires can lead to the atmospheric transport of macronutrients and bio-essential trace metals such as nitrogen and iron, respectively8,9,10. It has been suggested that the oceanic deposition of wildfire aerosols can relieve nutrient limitations and, consequently, enhance marine productivity11,12, but direct observations are lacking. Here we use satellite and autonomous biogeochemical Argo float data to evaluate the effect of 2019–2020 Australian wildfire aerosol deposition on phytoplankton productivity. We find anomalously widespread phytoplankton blooms from December 2019 to March 2020 in the Southern Ocean downwind of Australia. Aerosol samples originating from the Australian wildfires contained a high iron content and atmospheric trajectories show that these aerosols were likely to be transported to the bloom regions, suggesting that the blooms resulted from the fertilization of the iron-limited waters of the Southern Ocean. Climate models project more frequent and severe wildfires in many regions1,2,3. A greater appreciation of the links between wildfires, pyrogenic aerosols13, nutrient cycling and marine photosynthesis could improve our understanding of the contemporary and glacial–interglacial cycling of atmospheric CO2 and the global climate system.Analyses of satellite aerosol observations used in this study were produced with the Giovanni online data system, developed and maintained by the NASA GES DISC. We thank SeaWiFS and MODIS mission scientists and associated NASA personnel for the production of the data used in this research effort. The BGC-Argo data were collected and made freely available by the International Argo Program and the national programs that contribute to it (http://www.argo.ucsd.edu, http://argo.jcommops.org). The Argo Program is part of the Global Ocean Observing System (https://doi.org/10.17882/42182). W.T. is supported by the Harry H. Hess Postdoctoral Fellowship from Princeton University. N.C. is supported by the “Laboratoire d’Excellence” LabexMER (ANR‐10‐LABX‐19) and co-funded by a grant from the French government under the program “Investissements d’Avenir”. S.B. acknowledges the AXA Research Fund for the support of the long-term research line on Sand and Dust Storms at the Barcelona Supercomputing Center (BSC) and CAMS Global Validation (CAMS-84). P.G.S., J.L., M.M.G.P. and A.R.B. are supported by the Australian Research Council Discovery Projects scheme (DP190103504). P.G.S. and J.W. are supported by the Australian Research Council Centre of Excellence for Climate Extremes (CLEX: CE170100023). J.L. is supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 754433. A.R.B. is supported by the Australian Research Council Future Fellowship scheme (FT130100037). R.M. is supported by the CSIRO Decadal Climate Forecasting Project. We thank M. Strzelec, M. East, T. Holmes, M. Corkill, S. Meyerink and the Wellington Park Management Trust for help with installation and sampling the Tasmanian aerosol time-series station; A. Townsend for iron aerosol analyses by ICPMS at the University of Tasmania; and A. Benedetti and S. Remy for providing insights on the validation of aerosol reanalysis.Peer Reviewed"Article signat per 15 autors/es: Weiyi Tang, Joan Llort, Jakob Weis, Morgane M. G. Perron, Sara Basart, Zuchuan Li, Shubha Sathyendranath, Thomas Jackson, Estrella Sanz Rodriguez, Bernadette C. Proemse, Andrew R. Bowie, Christina Schallenberg, Peter G. Strutton, Richard Matear & Nicolas Cassar"Postprint (author's final draft
Seasonality of downward carbon export in the Pacific Southern Ocean revealed by multi-year robotic observations
At high latitudes, the biological carbon pump, which exports organic matter from the surface ocean to the interior, has been attributed to the gravitational sinking of particulate organic carbon. Conspicuous deficits in ocean carbon budgets challenge this as a sole particle export pathway. Recent model estimates revealed that particle injection pumps have a comparable downward flux of particulate organic carbon to the biological gravitational pump, but with different seasonality. To date, logistical constraints have prevented concomitant and extensive observations of these mechanisms. Here, using year-round robotic observations and recent advances in bio-optical signal analysis, we concurrently investigated the functioning of two particle injection pumps, the mixed layer and eddy subduction pumps, and the gravitational pump in Southern Ocean waters. By comparing three annual cycles in contrasting physical and biogeochemical environments, we show how physical forcing, phytoplankton phenology and particle characteristics influence the magnitude and seasonality of these export pathways, with implications for carbon sequestration efficiency over the annual cycle
Open-Source Bioinformatic Pipeline to Improve PMS2 Genetic Testing Using Short-Read NGS Data
The molecular diagnosis of mismatch repair- de fi cient cancer syndromes is hampered by difficulties fi culties in sequencing the PMS2 gene, mainly owing to the PMS2CL pseudogene. Next-generation sequencing short reads cannot be mapped unambiguously by standard pipelines, compromising variant calling accuracy. This study aimed to provide a refined fi ned bioinformatic pipeline for PMS2 mutational analysis and explore PMS2 germline pathogenic variant prevalence in an unselected hereditary cancer (HC) cohort. PMS2 mutational analysis was optimized using two cohorts: 192 unselected HC patients for assessing the allelic ratio of paralogous sequence variants, and 13 samples enriched with PMS2 (likely) pathogenic variants screened previously by long-range genomic DNA PCR amplification. fi cation. Reads were forced to align with the PMS2 reference sequence, except those corresponding to exon 11, where only those intersecting gene-specific fi c invariant positions were considered. Afterward, the refined fi ned pipeline's accuracy was validated in a cohort of 40 patients and used to screen 5619 HC patients. Compared with our routine diagnostic pipeline, the PMS2_vaR pipeline showed increased technical sensitivity (0.853 to 0.956, respectively) in the validation cohort, identifying all previously PMS2 pathogenic variants found by long-range genomic DNA PCR amplification. fi cation. Fifteen HC cohort samples carried a pathogenic PMS2 variant (15 of 5619; 0.285%), doubling the estimated prevalence in the general population. The refined fi ned open-source approach improved PMS2 mutational analysis accuracy, allowing its inclusion in the routine next-generation sequencing pipeline streamlining PMS2 screening. (J Mol Diagn 2024, 26: 727-738; https://doi.org/10.1016/j.jmoldx.2024.05.005
Complete Loss of EPCAM Immunoexpression Identifies EPCAM Deletion Carriers in MSH2-Negative Colorectal Neoplasia
The use of epithelial cell adhesion molecule (EPCAM) immunohistochemistry (IHC) is not included in the colorectal cancer (CRC) screening algorithm to detect Lynch syndrome (LS) patients. The aim of the present study was to demonstrate that EPCAM IHC is a useful tool to guide the LS germ-line analysis when a loss of MSH2 expression was present. We retrospectively studied MSH2 and EPCAM IHC in a large series of 190 lesions composed of malignant neoplasms (102), precursor lesions of gastrointestinal (71) and extra-gastrointestinal origin (9), and benign neoplasms (8) from different organs of 71 patients suspicious of being LS due to MSH2 alterations. LS was confirmed in 68 patients, 53 with MSH2 mutations and 15 with EPCAM 3'-end deletions. Tissue microarrays were constructed with human normal tissues and their malignant counterparts to assist in the evaluation of EPCAM staining. Among 154 MSH2-negative lesions, 17 were EPCAM-negative, including 10 CRC and 7 colorectal polyps, and 5 of them showed only isolated negative glands. All lesions showing a lack of EPCAM expression belonged to patients with EPCAM 3'-end deletions. EPCAM IHC is a useful screening tool, with 100% specificity to identify LS patients due to EPCAM 3'-end deletions in MSH2-negative CRC and MSH2-negative colorectal polyps
Mammographic density and breast cancer in women from high risk families
Introduction: Mammographic density (MD) is one of the strongest determinants of sporadic breast cancer (BC). In this study, we compared MD in BRCA1/2 mutation carriers and non-carriers from BRCA1/2 mutation-positive families and investigated the association between MD and BC among BRCA1/2 mutation carriers per type of mutation and tumor subtype. Methods: The study was carried out in 1039 female members of BRCA1 and BRCA2 mutation-positive families followed at 16 Spanish Genetic Counseling Units. Participants' density was scored retrospectively from available mammograms by a single blinded radiologist using a 5-category scale (75 %). In BC cases, we selected mammograms taken prior to diagnosis or from the contralateral breast, whereas, in non-cases, the last screening mammogram was evaluated. MD distribution in carriers and non-carriers was compared using ordinal logistic models, and the association between MD and BC in BRCA1/2 mutation carriers was studied using logistic regression. Huber-White robust estimators of variance were used to take into account correlations between family members. A similar multinomial model was used to explore this association by BC subtype. Results: We identified and scored mammograms from 341 BRCA1, 350 BRCA2 mutation carriers and 229 non-carriers. Compared to non-carriers, MD was significantly lower among BRCA2 mutation carriers (odds ratio (OR) =0.71; P-value=0.04), but not among BRCA1 carriers (OR=0.84; P-value=0.33). MD was associated with subsequent development BC (OR per category of MD=1.45; 95 % confidence interval=1.18-1.78, P-value<0.001), with no significant differences between BRCA1 and BRCA2 mutation carriers (P-value=0.48). Finally, no statistically significant differences were observed in the association of MD with specific BC subtypes. Conclusions: Our study, the largest to date on this issue, confirms that MD is an independent risk factor for all BC subtypes in either BRCA1 and BRCA2 mutation carriers, and should be considered a phenotype risk marker in this context
Phénologie, méchanismes et changement future du cycle saisonnier phytoplanctonique.
DOI: 10.13140/RG.2.1.1922.0003Primary production (PP) in the Southern Ocean (SO) plays a crucial role on atmospheric carbon uptake. PP in this ocean is highly iron-limited and presents a marked seasonal cycle. Such a seasonal cycle has a strong productive phase in late winter, called bloom, which distribution and intensity is highly variable. My PhD focus on two specific aspects of the PP in the SO: first, the mechanisms that drive such a bloom and its dynamics and, second, the elements able to control the bloom intensity at present and in the future. The first aspect (bloom phenology and mechanisms) was addressed by setting up a mechanistic approach based on a novel model configuration: a complex biogeochemical model (PISCES) forced by a 1D idealised physical framework. This methodology allowed me to conciliate the different bloom formation theories and to identify the SO bloom specificities. Moreover, I proposed how to use different bloom detection criteria to properly identify bloom from observations. Such criteria were then tested in a complementary observation-based approach (with satellite and in-situ data) to characterise different bloom phenologies and its spatial distribution in the SO. The second aspect (bloom intensity and future change) was also addressed by a twofold approach. First, using the 1D model, I studied how seasonal variability of vertical mixing combine light and Fe limitation to drive PP. Secondly, I used such an analysis to interpret PP trends observed in 8 coupled model climatic projections (CMIP5 models). My PhD thesis results allow for a better understanding of the physical and biological processes controlling phytoplankton growth. My conclusions also suggest how an alteration of these processes by Climate Change may influence PP in the whole SO, a key region for future climate evolution.La production primaire (PP) dans l’Océan Austral joue un rôle crucial dans la capacité des océans à absorber le carbon atmosphérique. Elle est caract éris ée par une forte limitation en Fer et par un cycle saisonnier très marqué, présentant un bloom planctonique en fin d’hiver, plus ou moins intense selon les régions. Ma thèse est centrée sur la compréhension des mécanismes qui contrôlent ce bloom et sa variabilité, ainsi que sur les éléments, présents et futurs, qui contrôlent son intensité. J’ai abordé le premier aspect (phénologie et mécanismes) en mettant en place une approche mécaniste basée sur une nouvelle configuration du modèle biogéochimique PISCES forcé par un environnement physique 1D idéalisé. Cette méthodologie m’a permis de réconcilier les différentes théories sur la formation des blooms aux hautes-latitudes, d’identifier les spécificités du bloom de l’Océan Austral et de proposer des critères adaptés à sa détection dans les observations. En outre, les résultats de cette étude de modélisation ont été confrontés à ceux issues d’une deuxième approche, basée sur des observations satellitaires, ce qui a permis la localisation géographique des différentes phénologies de bloom que j’ai identifiées dans l’Océan Austral. Pour répondre au deuxième aspect (altération et changements futurs), j’ai également suivi une double approche. J’ai d’abord examiné comment les limitations par la lumière et par le fer se combinent, via la variabilité du cycle saisonnier du mélange vertical, et pilotent ainsi la production primaire dans l’Océan Austral actuel à l’aide de la configuration idéalisée présentée plus haut. Dans un deuxième temps, cette analyse a permis d’aider à l’interprétation des variations de PP observées dans les projections climatiques issues de 8 modèles couplés (CMIP5). L’ensemble de mes résultats permet de mieux comprendre les processus physiques et biologiques qui contrôlent la croissance du phytoplancton dans l’Océan Austral et d’appréhender comment la modification de ces processus peut entraîner des altérations de la PP dans une région clé pour l’évolution future du climat
Phénologie, mécanismes et changement futur du cycle saisonnier phytoplanktonique dans l'Océan Austral
La production primaire (PP) dans l'Océan Austral joue un rôle crucial dans la capacité des océans à absorber le carbon atmosphérique. Elle est caractérisée par une forte limitation en Fer et par un cycle saisonnier très marqué, présentant un bloom planctonique en fin d'hiver, plus ou moins intense selon les régions. Ma thèse est centrée sur la compréhension des mécanismes qui contrôlent ce bloom et sa variabilité, ainsi que sur les éléments, présents et futurs, qui contrôlent son intensité. J'ai abordé le premier aspect (phénologie et mécanismes) en mettant en place une approche mécaniste basée sur une nouvelle configuration du modèle biogéochimique PISCES forcé par un environnement physique 1D idéalisé. Cette méthodologie m'a permis de réconcilier les différentes théories sur la formation des blooms aux hautes-latitudes, d'identifier les spécificités du bloom de l'Océan Austral et de proposer des critères adaptés à sa détection dans les observations. En outre, les résultats de cette étude de modélisation ont été confrontés à ceux issues d'une deuxième approche, basée sur des observations satellitaires, ce qui a permis la localisation géographique des différentes phénologies de bloom que j'ai identifiées dans l'Océan Austral. Pour répondre au deuxième aspect (altération et changements futurs), j'ai également suivi une double approche. J'ai d'abord examiné comment les limitations par la lumière et par le fer se combinent, via la variabilité du cycle saisonnier du mélange vertical, et pilotent ainsi la production primaire dans l'Océan Austral actuel à l'aide de la configuration idéalisée présentée plus haut. Dans un deuxième temps, cette analyse a permis d'aider à l’interprétation des variations de PP observées dans les projections climatiques issues de 8 modèles couplés (CMIP5). L'ensemble de mes résultats permet de mieux comprendre les processus physiques et biologiques qui contrôlent la croissance du phytoplancton dans l'Océan Austral et d'appréhender comment la modification de ces processus peut entraîner des altérations de la PP dans une région clé pour l'évolution future du climat.Primary production (PP) in the Southern Ocean (SO) plays a crucial role on atmospheric carbon uptake. PP in this ocean is highly iron-limited and presents a marked seasonal cycle. Such a seasonal cycle has a strong productive phase in late winter, called bloom, which distribution and intensity is highly variable. My PhD focus on two specific aspects of the PP in the SO: first, the mechanisms that drive such a bloom and its dynamics and, second, the elements able to control the bloom intensity at present and in the future. The first aspect (bloom phenology and mechanisms) was addressed by setting up a mechanistic approach based on a novel model configuration: a complex biogeochemical model (PISCES) forced by a 1D idealised physical framework. This methodology allowed me to conciliate the different bloom formation theories and to identify the SO bloom specificities. Moreover, I proposed how to use different bloom detection criteria to properly identify bloom from observations. Such criteria were then tested in a complementary observation-based approach (with satellite and in-situ data) to characterise different bloom phenologies and its spatial distribution in the SO. The second aspect (bloom intensity and future change) was also addressed by a twofold approach. First, using the 1D model, I studied how seasonal variability of vertical mixing combine light and Fe limitation to drive PP. Secondly, I used such an analysis to interpret PP trends observed in 8 coupled model climatic projections (CMIP5 models). My PhD thesis results allow for a better understanding of the physical and biological processes controlling phytoplankton growth. My conclusions also suggest how an alteration of these processes by Climate Change may influence PP in the whole SO, a key region for future climate evolution
Un territori ordenat, equilibrat i amb les dotacions necessàries: pla director de coordinació del Delta de l'Ebre
L'objectiu del Pla director del delta de l'Ebre és establir una ordenació global del territori que potencïi les explotacions agrícoles, ramaderes i aqüícoles, i que creï els serveis necessaris per a una explotació turística i una implantació industrial, tot garantint el respecte i la protecció d'un patrimoni natural extremadament valuós. El document del Pla director del delta de l'Ebre recull les preexistències actuals, tant físiques com normatives, estableix un model comú d'ocupació del territori, preveu els centres d'activitats productives necessaris i la seva ubicació per una explotació respectuosa dels recursos naturals, i fixa les prioritats bàsiques pel que fa a les infraestructures