Potential impact of subsonic and supersonic aircraft exhaust on water vapor in the lower stratosphere assessed via a trajectory model

We employ a trajectory model to assess the impact on the stratosphere of water vapor present in the exhaust of subsonic and a proposed fleet of supersonic aircraft. Air parcels into which water vapor from aircraft exhaust has been injected are run through a 6-year simulation in the trajectory model using meteorological data from the UKMO analyses with emissions dictated by the standard 2015 emissions scenario. For the subsonic aircraft, our results suggest maximum enhancements of ~150 ppbv just above the Northern Hemisphere tropopause and of much less than 50 ppbv in most other regions. Inserting the perturbed water vapor profiles into a radiative transfer model, but not considering the impact of additional cirrus formation resulting from emissions by subsonic aircraft, we find that the impact of subsonic water vapor emissions on the radiative balance is negligible. For the supersonic case, our results show maximum enhancements of ~1.5 ppmv in the tropical stratosphere near 20 km. Much of the remaining stratosphere between 12 and 25 km sees enhancements of greater than 0.1 ppmv, although enhancements above 35 km are generally less than 50 ppbv, in contrast to previous 2-D and 3-D model studies. Radiative calculations based upon these projected water vapor perturbations indicate they may cause a nonnegligible impact on tropical temperature profiles. Since our trajectory model includes no chemistry and our radiative calculations use the most extreme water vapor perturbations, our results should be viewed as upper limits on the potential impacts

Potential impact of subsonic and supersonic aircraft exhaust on water vapor in the lower stratosphere assessed via a trajectory model

We employ a trajectory model to assess the impact on the stratosphere of water vapor present in the exhaust of subsonic and a proposed fleet of supersonic aircraft. Air parcels into which water vapor from aircraft exhaust has been injected are run through a 6-year simulation in the trajectory model using meteorological data from the UKMO analyses with emissions dictated by the standard 2015 emissions scenario. For the subsonic aircraft, our results suggest maximum enhancements of ~150 ppbv just above the Northern Hemisphere tropopause and of much less than 50 ppbv in most other regions. Inserting the perturbed water vapor profiles into a radiative transfer model, but not considering the impact of additional cirrus formation resulting from emissions by subsonic aircraft, we find that the impact of subsonic water vapor emissions on the radiative balance is negligible. For the supersonic case, our results show maximum enhancements of ~1.5 ppmv in the tropical stratosphere near 20 km. Much of the remaining stratosphere between 12 and 25 km sees enhancements of greater than 0.1 ppmv, although enhancements above 35 km are generally less than 50 ppbv, in contrast to previous 2-D and 3-D model studies. Radiative calculations based upon these projected water vapor perturbations indicate they may cause a nonnegligible impact on tropical temperature profiles. Since our trajectory model includes no chemistry and our radiative calculations use the most extreme water vapor perturbations, our results should be viewed as upper limits on the potential impacts

Nucleic Acid-Loaded Lipid Nanoparticle Interactions with Model Endosomal Membranes

[Image: see text] Lipid nanoparticles (LNPs) are important delivery systems for RNA-based therapeutics, yet the mechanism of their interaction with endosomal membranes remains unclear. Here, the interactions of nucleic acid-loaded LNPs that contain an ionizable lipid with models of the early and late endosomal membranes are studied, for the first time, using different reflectometry techniques. Novel insight is provided with respect to the subphase pH, the stage of the endosome, and the nature of the nucleic acid cargo. It is found that the insertion of lipids from the LNPs into the model membrane is greatest at pH 6.5 and 5.5, whereas at higher pH, lipid insertion is suppressed with evidence instead for the binding of intact LNPs, demonstrating the importance of the pH in the fusion of LNPs undergoing the endosomal pathway. Furthermore, and independently of the pH, the effect of the early- versus late-stage endosomal models is minimal, suggesting that the increased fluidity and anionic nature of the late endosome has little effect on the extent of LNP interaction. Last, there is greater nucleic acid delivery from LNPs containing mRNA than Poly(A), indicating that the extent of interaction can be tuned according to the nature of the nucleic acid cargo. Such new information on the relative impact of factors influencing nucleic acid delivery by LNP interactions with endosomal membranes is important in the design and tuning of vehicles with improved nucleic acid delivery capacities

Psychological States Underlying Excellent Performance in Sport: Toward an Integrated Model of Flow and Clutch States

This study investigated the psychological states underlying excellent performance in 26 athletes (Mage = 29 years, SD = 7.7) across a range of sports (team, net/wall, sprint, endurance, adventure) and standards (world class to recreational). Participants were primarily interviewed on average 4 days after excellent performances. The data were analyzed thematically. Distinct states of flow and clutch were reported, each of which occurred through separate contexts and processes, while athletes also transitioned between states during performance. These findings extend current knowledge of the psychology of excellent performance and are discussed in terms of implications for future research and applied practice

The localization and phosphorylation of p47 are important for Golgi disassembly–assembly during the cell cycle

In mammalian cells, the Golgi apparatus is disassembled at the onset of mitosis and reassembled at the end of mitosis. This disassembly–reassembly is generally believed to be essential for the equal partitioning of Golgi into two daughter cells. For Golgi disassembly, membrane fusion, which is mediated by NSF and p97, needs to be blocked. For the NSF pathway, the tethering of p115-GM130 is disrupted by the mitotic phosphorylation of GM130, resulting in the inhibition of NSF-mediated fusion. In contrast, the p97/p47 pathway does not require p115-GM130 tethering, and its mitotic inhibitory mechanism has been unclear. Now, we have found that p47, which mainly localizes to the nucleus during interphase, is phosphorylated on Serine-140 by Cdc2 at mitosis. The phosphorylated p47 does not bind to Golgi membranes. An in vitro assay shows that this phosphorylation is required for Golgi disassembly. Microinjection of p47(S140A), which is unable to be phosphorylated, allows the cell to keep Golgi stacks during mitosis and has no effect on the equal partitioning of Golgi into two daughter cells, suggesting that Golgi fragmentation-dispersion may not be obligatory for equal partitioning even in mammalian cells

An Experimental Investigation of the Adsorption of a Phosphonic Acid on the Anatase TiO2(101) Surface

A combination of synchrotron radiation photoelectron spectroscopy and near-edge X-ray absorption fine structure (NEXAFS) spectroscopy has been used to study the adsorption of phenylphosphonic acid (PPA) on anatase TiO2(101) single crystal at coverages of 0.15 monolayer (ML) and 0.85 ML. The photoelectron spectroscopy data suggest that at 0.15 ML coverage PPA adsorbs in a bidentate geometry following deprotonation of both phosphonate hydroxyl groups, leaving the P═O group unbound. At 0.85 ML there is a shift to a mixed bidentate/monodentate binding mode. The carbon K-edge NEXAFS spectra were recorded at two azimuths. Our calculations show that for PPA on anatase TiO2(101) the phenyl ring is oriented 65 ± 4° away from the surface plane with an azimuthal twist of 57 ± 11° away from the [101] azimuth

An Analysis of Tropical Transport: Influence of the Quasi-biennial Oscillation

An analysis of over 4 years of Upper Atmosphere Research Satellite (UARS) measurements of CH4, HF, O3, and zonal wind are used to study the influence of the quasi-biennial oscillation (QBO) on constituent transport in the tropics. At the equator, spectral analysis of the Halogen Occultation Experiment (HALOE) and Microwave Limb Sounder (MLS) observations reveals QBO signals in constituent and temperature fields at altitudes between 20 and 45 km. Between these altitudes, the location of the maximum QBO amplitude roughly corresponds with the location of the largest vertical gradient in the constituent field. Thus, at 40 km where CH4 and HF have strong vertical gradients, QBO signals are correspondingly large, while at lower altitudes where the vertical gradients are weak, so are the QBO variations. Similarly, ozone, which is largely under dynamical control below 30 km in the tropics, has a strong QBO signal in the region of sharp vertical gradients (∼28 km) below the ozone peak. Above 35 km, annual and semi-annual variations are also found to be important components of the variability of long-lived tracers. Therefore, above 30 km, the variability in CH4 and HF at the equator is represented by a combination of semiannual, annual, and QBO timescales. A one-dimensional vertical transport model is used to further investigate the influence of annual and QBO variations on tropical constituent fields. QBO-induced vertical motions are calculated from observed high resolution Doppler imager (HRDI) zonal winds at the equator, while the mean annually varying tropical ascent rate is obtained from the Goddard two-dimensional model. Model simulations of tropical CH4 confirm the importance of both the annual cycle and the QBO in describing the HALOE CH4 observations above 30 km. Estimates of the tropical ascent rate and the variation due to the annual cycle and QBO are also discussed

Comparing the dynamics of party leadership survival in Britain and Australia: Brown, Rudd and Gillard

This article examines the interaction between the respective party structures of the Australian Labor Party and the British Labour Party as a means of assessing the strategic options facing aspiring challengers for the party leadership. Noting the relative neglect within the scholarly literature on examining forced exits that occur; and attempted forced exits that do not occur, this article takes as its case study the successful forced exits of Kevin Rudd and Julia Gillard, and the failure to remove Gordon Brown. In doing so the article challenges the prevailing assumption that the likely success of leadership evictions are solely determined by the leadership procedures that parties adopt. Noting the significance of circumstances and party cultures, the article advances two scenarios through which eviction attempts can be understood: first, forced exits triggered through the activation of formal procedures (Rudd and Gillard); second, attempts to force an exit by informal pressures outside of the formal procedures which are overcome by the incumbent (Brown)

Race, Care Seeking, and Utilization for Chronic Back and Neck Pain: Population Perspectives

We analyzed a statewide survey of individuals with chronic back and neck pain to determine whether prevalence and care use varied by patient race or ethnicity. We conducted a telephone survey of a random sample of 5,357 North Carolina households in 2006. Adults with chronic (>3 months duration or >24 episodes of pain per year), impairing back or neck pain were identified and were asked to complete a survey about their health and care utilization. 837 respondents (620 white, 183 black, 34 Latino) reported chronic back or neck pain. Whites and blacks had similar rates of chronic back pain. Back pain prevalence was lower in Latinos (10.4% [9.3–11.6] vs 6.3% [3.8–8.8] ), likely due to their younger age; and the prevalence of chronic, disabling neck pain was lower in blacks (2.5% [1.9–3.1] vs 1.1% [0.04–1.9]). Blacks had higher pain scores in the previous 3 months (5.2 vs 5.9 p<0.05), and higher Roland disability scores (0–23 point scale): 14.2 vs 16.8, p<0.05. Care seeking was similar among races (83% white, 85% black, 72% Latino). Use of opioids was also similar between races, at 49% for whites, 52% for blacks, and trended lower at 35% for Latinos. We found few racial/ethnic differences in care-seeking, treatment use, and use of narcotics for the treatment of chronic back and neck pain