The Effect of PGF2α Injection on Post-Thaw Motility in Sperm of Nubian Goats

This study aims to determine the effect of PGF2α injection on the post-thaw motility (PTM) in sperm of Nubian goats. Three male Nubian goats (3-4 years) with good reproductive ability were used. This study used a 3 x 3 Latin square design. The experimental animals received a physiological NaCl injection as a control (P1); 37.5 mg of PGF2α (P2), and 75 mg of PGF2α (P3). Semen was collected using an artificial vagina with one-week storage intervals between treatments. The collected semen was then diluted and frozen using a simple freezing method. Observation of semen quality before freezing included macroscopic and microscopic examinations. Macroscopic examination consisted of volume, pH, color, odor, and consistency, while microscopic examination consisted of motility, concentration, viability, and abnormality. PTM examination was done by mixing a drop of sperm suspension and one drop of physiological NaCl on an object glass and covered prior to observation under microscope.. The results were analyzed using a Latin square pattern variant analysis, followed by Duncan's test. The PTM values of sperm (%) of Nubian goats in P1, P2, and P3 respectively are 28.71±10.24, 50.03±13.70, and 54.84±12.04 (P<0.05). Injection of PGF2α to Nubian goats by injection increased the PTM.

DETECTION OF SERUM MACRO MINERALS CONCENTRATION IN REPEAT BREEDING ACEH CATTLE

The purpose of this study was to determine the macro minerals concentration in serum of repeat breeding (RB) Aceh cattle. In this study 16 Aceh cattle were examined; they consisted of 7 fertile Aceh cattle (P1) and 9 Aceh cattle with RB (P2), all of which were 3-8 years old with abody condition score (BCS) of 3-4. Serum collection was carried out for examining the level of serum minerals, including magnesium, phosphorus, sodium, potassium, chloride, and calcium. Data was analysed using T-test. The levels of magnesium, phosphorus, sodium,potassium, chloride, and calcium in P1 vs P2 were 2.18±0.60 vs. 2.20±0.34 mg/dL; 6.18±1.34 vs. 6.48±0.74 mg/dL; 142.71±5.09 vs.142.44±2.29 mmol/L; 4.81±0.76 vs. 4.76±0.51 mmol/L; 104.57±4.35 vs. 107.67±7.36 mmol/L; and 9.07±0.45 vs. 9.90 ± 0.60 mg/dL (P0.05), respectively. It was concluded that the concentration of serum macro minerals do not affect the incidence of RB in Aceh cattle

The Effect of Red Betel Leaf (Piper crocatum) and Moringa Leaf Extracts on Endometritis Levels in Aceh Cows

This study aims to determine the ability of red betel leaf (Piper crocatum) or Moringa leaf extracts to reduce the endometritis level in Aceh cattle. In this study, six Aceh cows aged 3-5 years, weighing 150-250 kg from the Experimental Animal Technical Implementation Unit of Faculty of Veterinary Medicine of Syiah Kuala University were used. The cows were divided into two treatment groups, namely cows with endometritis that were given red betel leaf extract (T1) and cows with endometritis that were given Moringa leaf extract (T2). Examination of the endometritis levels was carried out before and after treatment using the White Side Test (WST) method. The collection of estrus cervical mucus was needed for the WST examination, and heat induction was performed with prostaglandin F2 alpha (PGF2α) at a dose of 25 μg. Collection of cervical mucus was performed 8-12 hours after the initiation of heat. All cows with endometritis were given intrauterine extracts of red betel leaves or Moringa leaves at a concentration of 20% every 24 hours for a week at a solution volume of 20 ml. The data were then analyzed using a paired t test. The mean endometritis levels before and after treatment on K1 vs. K2 were 3.0 and 1.7 vs. 2.7 and 2.7, respectively (P<0.05). It was concluded that red betel leaf extract at a concentration of 20% was more effective in reducing the endometritis level of Aceh cows than Moringa leaf extract

Pengaruh Pemberian PGF2α terhadap Peningkatan Kualitas Spermatozoa Kambing Boerka

Usaha untuk meningkatkan produktivitas ternak kambing Boerka di antaranya dengan melakukan inseminasi buatan (IB). Untuk meningkatkan kualitas spermatozoa yang akan digunakan untuk IB maka diberikan hormon prostaglandin F2 alfa (PGF2α). Penelitian ini bertujuan mengetahui pengaruh pemberian PGF2α terhadap peningkatan motilitas spermatozoa kambing Boerka. Dalam penelitian digunakan 3 ekor kambing Boerka yang berumur ±2-3 tahun. Pelaksanaan perlakuan dirancang menggunakan pola latin square 3 x 3 sehingga hewan akan menerima suntikan P1 (1,5 ml NaCl fisiologis), P2 (37,5 μg PGF2α), dan P3 (75 μg PGF2α) dengan interval waktu perlakuan adalah 30 menit sebelum koleksi semen. Sampel semen dikoleksi dengan menggunakan vagina buatan dan diamati warna, konsistensi, volume, konsentrasi, motilitas, viabilitas, dan motilitas spermatozoa. Motilitas spermatozoa diamati setelah 4 jam di dalam refrigerator. Data warna dan konsistensi semen dilaporkan secara deskriptif, sedangkan volume, motilitas semen segar, dan motilitas spermatozoa setelah 4 jam di dalam refrigerator dianalisis dengan analisis varian pola bujur sangkar latin (RSBL) yang dilanjutkan dengan uji Duncan. Hasil pengamatan menunjukkan bahwa warna dan konsistensi semen yang dikoleksi pada semua kelompok perlakuan adalah krem dengan konsistensi kental. Rataan (±SD) volume semen; konsentrasi spermatozoa (106/ml); motilitas semen segar (%); dan motilitas semen setelah penyimpanan pada P1 vs P2 vs P3 masing-masing adalah 0,90±0,4 vs 0,70±0,3 vs 0,90±0,3 ml (P&gt;0,05); 2303,33±327,15 vs 2336,67±332,91 vs 2576,67±261,02 (P&gt;0,05); 84,00±5,1 vs 73,33±11,54 vs 80,00±0,0% (P&gt;0,05); 63,67±4,5 vs 53,33±4,7 vs 66,67±2,2% (P&lt;0,05). Disimpulkan bahwa pemberian 75 μg PGF2α dapat meningkatkan motilitas spermatozoa kambing Boerka setelah penyimpanan dalam regrigerator selama 4 jam

Pemodelan Matematik untuk Menentukan Faktor-faktor Penyebab Repeat Breeding pada Sapi Aceh

Penelitian ini bertujuan membuat pemodelan untuk diagnosis repeat breeding (RB) pada sapi aceh berdasarkan intensitas estrus, profil hormonal, profil biokimia darah, dan jumlah infeksi bakteri pada saluran uterus. Hewan yang digunakan pada penelitian ini adalah 16 ekor sapi aceh yang terdiri atas 7 ekor sapi aceh fertil dan 9 ekor sapi aceh RB, yang berumur 3-8 tahun dengan skor kondisi tubuh (BCS) 3-4. Seluruh sapi aceh fertil dan RB dilakukan sinkronisasi estrus menggunakan hormon PGF2α dengan pola penyuntikan ganda dengan interval 11 hari. Setelah penyuntikan PGF2α, intensitas estrus diamati 3 kali sehari yakni pada pukul 08.00, 12.00, dan 16.00 WIB, masing-masing pengamatan selama 20 menit. Koleksi serum dilakukan pada pagi hari (jam 07.00-09.00 WIB). Koleksi serum dilakukan untuk pemeriksaan kadar hormon estradiol dan progesteron menggunakan teknik enzyme linked immunosorbent assay (ELISA). Selain itu, sampel darah juga digunakan untuk pemeriksaan profil biokimia darah. Koleksi sampel bakteri dilakukan dengan metode swab uterus. Hasil pemodelan diagnosis RB pada sapi aceh diperoleh model matematis regresi linear sebagai berikut : Y= a + bX1 + bX2 .............+ bX11S/C = -5.28 + 1,27X1 - 0,69X2 - 0,99X3 - 0,23X4 + 2,28X5 – 0,53X6 + 0,71X7 - 0,29X8 + 0,09X9 + 3,04X10 Berdasarkan hasil dari pemodelan diagnosis RB pada sapi aceh menunjukkan bahwa penyebab utama RB pada sapi aceh adalah infeksi bakteri pada uterus yang kemungkinan mengakibatkan sapi tersebut mengalami stres yang ditandai dengan tingginya kadar glukosa dalam darah. Selain itu, RB pada sapi aceh juga dipengaruhi oleh ketidakseimbangan nutrisi dan hormonal yang mengakibatkan intensitas estrus menjadi rendah

Accuracy of early pregnancy diagnosis using interferon-tau (IFN-Ï„) in Aceh cows

Interferon-tau (IFN-τ) is a pregnancy signal produced by embryonic trophoblast cells at the time of implantation in a mammal's endometrial wall, which is useful for conveying a message that the mammal can accept the presence of the foreign object (embryo). This study aims to determine the accuracy of early pregnancy diagnosis in Aceh cows by measuring the concentration of IFN-τ. This study involved eight cows aged 3-5 years, weighing 150-250 kg, clinically healthy, and having normal reproduction (have had calves and at least two regular cycles). All cows were estrous-synchronized twice using PGF2ɑ at a dose of 5 ml with 11 days intervals before artificial insemination (AI). Serum collection was performed on days 14, 15, 16, 17, 18 after AI. Based on ultrasound examination, out of eight cows, four (50%) cows were diagnosed as pregnant, and four (50%) cows were diagnosed as not pregnant. Mean (± SD) IFN-τ of pregnant vs. non-pregnant cows on day 14, 15, 16, 17, and 18 were 14.96±8.65 pg/ml vs. 6.14±5.54 pg/mL; 16.74±5.28 pg/mL vs. 4.44±3.51 pg/mL; 14.33±5.9 pg/mL vs. 5.78±5.20 pg/mL; 13.87±5.42 pg/mL vs. 4.38±3.76 pg/mL; and 13.93±6, 16 pg/mL vs. 3.24±2.63 pg/mL, respectively. The lowest IFN-τ concentration in cows that were successfully pregnant was 7.88±1.84 pg/mL. The best timing of IFN-τ for pregnancy diagnosis was on day 15 after AI, with an accuracy of 87.5%, specificity of 100%, and sensitivity of 80%

Peningkatan Motilitas Spermatozoa Kambing Nubian Setelah Pemberian PGF2α dalam Pengencer Andromed

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian PGF2α dalam pengencer semen komersial (Andromed) terhadap peningkatan motilitas spermatozoa kambing Nubian. Penelitian ini menggunakan sampel semen yang dikoleksi dari 3 ekor kambing Nubian berumur 2-3 tahun menggunakan vagina buatan dan dievaluasi kualitasnya secara makroskopis dan mikroskopis. Setelah dievaluasi, sampel semen ditambahkan pengencer Andromed lalu dibagi atas 3 kelompok perlakuan, yaitu: P1; P2; dan P3 yang masing-masing ditambahkan NaCl fisiologis; 37,5 µg PGF2α; dan 75 µg PGF2α. Seluruh sampel disimpan dalam refrigerator selama 4 jam dan dilakukan pemeriksaan motilitas spermatozoa. Data yang diperoleh dianalisis dengan sidik ragam pola satu arah (ANOVA) dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan, bahwa motilitas spermatozoa (%) kambing Nubian pada P1; P2; dan P3 masing-masing adalah 26,33±5,5; 62,0±3,5; dan61,8±10,13 (P0,05). Disimpulkan bahwa penambahan PGF2α pada pengencer Andromed dapat meningkatkan motilitas spermatozoa kambing Nubian.  (The improvement of sperm motility in Nubian goat after PGF2α administration in andromed semen diluents) ABSTRACT. The study aims to determine the administration effect of PGF2α in a commercial semen diluents (Andromed) on improvement of Nubian goat sperm motility. This study used semen samples that collected from three Nubian goats aged 2-3 using artificial vagina and their quality evaluated macroscopically and microscopically. After evaluated, semen samples were added with Andromed diluents then divided into three groups (P1, P2, and P3) where each group was then added with 0,9% physiologic NaCl, 37.5 µg PGF2α, and 75 µg PGF2α, respectively and stored in a refrigerator for 4 hours and subsequently spermatozoa motility was examined. The data obtained were analyzed by one-way analysis of variance (ANOVA) and followed by Duncan test. The results showed that the spermatozoa motility (%) of Nubian goats at P1, P2, and P3 were 26.33±5.5, 62.0±3.5, and 61.8±10.13, respectively. Based on the statistical tests showed that the administration of PGF2α at P2 and P3 had a significant effect (P0,05) on the motility of spermatozoa of Nubian goats, but the motility decreased in P1. The conclusion of this study is the addition of PGF2α to Andromed diluents can increase the motility of spermatozoa of Nubian goats