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    Association of Growth Hormone Gen with KUB Chicken Productivity

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    This study aims to investigate the diversity of quantitative characteristics and GH genes, along with the association between GH genes and quantitative characteristics. The research material consisted of 96 KUB chickens aged DOC-2 months and corresponding blood samples. The t-test was utilized to determine differences in body weight, weight gain, and body measurements between male and female KUB chickens, and assess the diversity of the GH gene. T2-Hotelling analysis was employed to compare body measurements between male and female KUB chickens, while principal component analysis was used to identify size and shape characteristics. Male KUB chickens' average body weight, weight gain and body size were significantly (p<0.05) higher than females. The analysis of the growth hormone gene exhibited three genotypes: +/+ (0.51), +/- (0.35), and -/- (0.14). Additionally, two alleles were identified: (+) accounting for 0.68% and (-) accounting for 0.32%. The growth hormone gene MspI of KUB chickens demonstrated polymorphism, with X2 count (2.93) < X2 table 0.05 (3.84). The heterozygosity value in the KUB chicken population was 0.43, and the obtained PIC value was 0.38. The genotype +/+ of the growth hormone gene in KUB chickens was significantly higher (p<0.05) than genotypes +/- and -/-.In conclusion, male KUB chickens exhibited higher body weight, weight gain, and body measurements compared to female KUB chickens. The chest circumference served as the identifier for body size in both male and female KUB chickens, while the length of the upper body and tibia length distinguished the shape characteristics of male and female KUB chickens, respectively. KUB chickens' growth hormone gene MspI was associated with body weight, weight gain, and body measurements, with the (+/+) genotype being the most favorabl

    Embryo Development and Chick Performance of Local Chicken Following In-Ovo Injection of L-Arginine Into Local Chicken Eggs

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    This study aims to determine the effect of in-ovo injection of L-arginine into local chicken eggs on embryo development and chick performance of local chicken. A total of 160 eggs were incubated using semi-automatic incubator with temperature of 37-38C and relative humidity of 55-65%. The injected L-arginine solution has a concentration of 0.5% (m/v). In treatment was divided into 4 groups, the first treatment was without injection (control), the second treatment was the injection of 0.2 mL L-arginine solution 0.5% (m/v)/egg, the third treatment was the injection of 0.4 mL L-arginine solution. 0.5% (m/v)/egg, and fourth treatment was the injection of L-arginine solution 0.6 mL 0.5%(m/v)/egg. The results showed that embryo mortality and hatchability were lower with L-arginine injection treatment than without injection. All treatments showed no effect on extraembryonic fluid absorption, but there was an increase in embryo weight in the injection treatment 0.2, 0.4, 0.6 mL L-Arginine 0.5% /eggs 2,355 g, 2,577 g, 2,705, respectively. In conclusion, an in-ovo injection of L-arginine in local chicken eggs has a good effect (the beneficial effect) on embryo death and embryo performance. Injection of 0.4 ml L-Arginine 0.5% /eggs improved hatchability and Newly Hatched Chick Weight (NHCW)

    Physical Quality of Complete Pellets Feed Containing Peanut Waste (Arachis hypogaea) and Its Effects on Rabbit Growth Performance

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    This study aimed to evaluate the physical quality of complete pellets with the addition of peanut waste and the effect of its administration on the performance of rabbits. A total of 20 local male rabbits aged 12 weeks with an average body weight of 1335.60 ± 280.53 g are divided into five groups based on body weight, and then used in this study which is performed in randomized block design (RBD). The rabbits are fed on the commercial pellets as a control diet (P0) and feed pellets containing peanut waste at the level of 5% (P1), peanut waste 10% (P2), and peanut waste 15% (P3). The results show that the physical quality of the pellets was not significantly different (p>0.05) in all treatments. Feeding complete pellets with the addition of peanut waste rabbits performances show no significant difference in performance (p>0.05) in all treatments. In conclusion, complete pellets with the addition of peanut was can resemble the physical quality of commercial pellets (control), maintain performance

    Utilization of Different Concentration Sugarcane Molasses to the Quality of Goat Feces-Chicken Excreta-Coconut Husk Organic Liquid Fertilizer

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    Indonesia is an agricultural country that certainly has the potential for agricultural waste and by-products. Some of those in Indonesia are livestock manure, coconut husk, and sugarcane molasses. Utilization of this waste is limited, which is causing various environmental problems such as environmental pollution. The study is aimed to determine the quality of organic liquid fertilizer made by raw material from goat feces-chicken excreta-coconut husk with various sugarcane molasses concentrations (P0= 0%; P1= 5%; P3= 15%; P4= 20%; P5= 25%). The fermentation was carried out anaerobically for 21 days. The parameters of temperature, pH, organoleptic (color and odor), levels of C-organic, nitrogen (N) total, phosphorus (P) total, potassium (K) total, and biological tests were observed. Biological tests were conducted in the growth of mung bean (Vigna radiata) and spinach (Amaranthus tricolor). Data were analyzed using one-way ANOVA. Variations in the addition of molasses sugarcane did not affect the liquid fertilizer's temperature and pH during fermentation. However, the treatments had positive effect on organoleptic test, C-organic, N total, P total, K total, and biological tests. The addition of 15% sugarcane molasses (P3) showed a dominating performance toward most of the parameters tested. The P3 treatment produced liquid fertilizer with a pleasant smell, and the contents of C-organic, total N, total P, and total K were 2.12%, 0.25%, 0.13%, and 0.13%,  respectively. and produce spinach as a biological test with plant height, number of leaves, leaf width, stem diameter, and root length were 21.82 cm, 6.66 sheets, 3.59 cm, 4.09 mm, and 14.67 cm, respectively

    Innovative Barn Cattle for Microclimate Management through the Misting System

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    Cattle is meat-producing livestock of the large ruminant. The environmental conditions must remain in the thermoneutral zone because this affects livestock productivity. An environmental modification related to microclimate management is required through a misting system using a water sprinkler. The study was conducted in one of the barns of the Sekolah Peternakan Rakyat (SPR) Maju Bersama livestock group in Drokilo Village, Kedungadem District, Bojonegoro Regency, East Java. The purpose of this study was to analyze the microclimate management of the barn through the misting system in order to improve the physiological response of cattle. The variables observed were the air temperature, air humidity, Temperature Humidity Index (THI), respiratory frequency, heart rate, rectal temperature, and body surface temperature. Body surface temperatures were measured on the face, back, and rump. The analytical method used in this was unpaired T-test five replications. The results showed that the average microclimate data for barn cattle were: air temperature 27.40±0.59°C, air humidity 83.92±1.55%, and Temperature Humidity Index (THI) 79.59±1.06. The results showed that the average physiological response data for cattle were: heart rate 47.02±2.29 beats per minute, respiration rate 16.94±1.26 beats per minute, and rectal temperature 38.65±0.27°C. The results showed that the average body surface temperature data for the face was 37.03±0.38°C, the back was 36.33±0.59°C, and the rump was 36.28±0.68°C. The results showed that the treatment before and after the morning measurement water misting was significantly different (p<0.05) in all the observed variables. Water misting has the effect on decreasing the air temperature, humidity, Temperature Humidity Index (THI), respiratory rate, heart rate, body temperature, and rectal temperature

    Evaluation of the Physical Quality of Supplement Wafer and Application of Supplement on Layer Performance

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    This study aimed to evaluate the physical quality of the wafer supplement with different binders and evaluate the performance of laying hens fed the supplement wafer in drinking water. The rearing stage used 64 Isa Brown chickens raised for 12 weeks. Stage 1 of the experiment used a 3x4 Completely randomized design (T1: Pollard, T2: Cassava Flour, T3: Tapioca Flour Waste). Phase two of the study used a completely randomized design with two treatments x 4 replicates x 8 chickens (P0: without supplement wafers, P1: supplement wafers). The variables observed were the wafer's physical quality and Isa Brown's performance. Different adhesives in the manufacture of wafers produced no significant difference in water content (p>0.05) but significant differences (p0.05) feed consumption, drinking water consumption, Feed Conversion Ratio (FCR), egg weight, and daily egg production but did affect (p<0.05) chicken mortality. The conclusion is that wafers with the best physical quality use cassava flour binder, and giving wafer supplements in drinking water can reduce mortality

    Sustainability Indicators of Kalang Buffalo Production Systems in Swamp Ecosystems in South Kalimantan

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    This study aims to identify problems, identify and analyze stakeholders and determine indicators (qualitative and quantitative) for the sustainability of kalang buffalo production systems in swamp ecosystems in South Kalimantan. The kalang buffalo is a type of mud buffalo that is reared using the kalang system. In the local language, kalang is an enclosure built in the middle of swamp waters. This study is expected to be a source of information for academics, farmers, communities and policymakers in South Kalimantan Province regarding the strategic function of Kalang buffalo for the people of South Kalimantan. Data collection was done by describing the problem and determining relevant stakeholders. The literature study was conducted with expert discussion. FGDs were also conducted to explore important issues, followed by SWOT (Strengths, Weaknesses, Opportunities, and Threats) analysis and to determine relevant issues. Situation analysis and secondary data showed that the Kalang buffalo population is declining. Stakeholder identification identified the central and local governments as primary stakeholders; secondary stakeholders were scientists/academics, butchers, sellers, and consumers. The primary stakeholders selected were buffalo farmers, central and local governments, while secondary stakeholders consisted of scientists/academics, butchers, farmer sellers, and consumers. The selected indicators on the EES issue are economic indicators such as gross margin (GM), body weight, weaning weight, and reproduction (calf crop (%)); environmental indicators such as total grazing land use and waste disposal; and social indicators such total of buffalo, feeding procedures, mating system, cages, record keeping, time allocation for maintenance, social status and land use conflicts

    Reproductive Success Rate and Blood Urea Nitrogen Status of Garut Ewes Fed Sorghum-Indigofera in Majalengka District - West Java

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    The body's Blood Urea Nitrogen (BUN) concentration could be used as diagnostic information for interpreting animal nutritional status, improving nutritional management, and preventing metabolic disorders in ewes during the reproductive phase. This study aimed to evaluate the reproductive success rate and changes in the BUN status of Garut ewes fed sorghum-Indigofera in Majalengka District – West Java, Indonesia. Twenty-eight multiparous Garut ewes with an average body weight of 30 kg, aged 2-3 years or parity from 3rd to 4th, and non-pregnant were separated into two feed treatment groups; divided field grass (FG) and sorghum-Indigofera (SI) mixed feed. This study was designed using a Complete Randomized Design (CRD). After one month of feed acclimatization, all ewes were served an estrus synchronization protocol and mated naturally. A pregnancy test was carried out on the 28th day after mating using ultrasonography (USG). Seven blood samples were taken (1 time in the pre-mating phase and three times each in the pregnancy and lactation phases), and the BUN parameter was analyzed. The results showed that SI feed increased the number of ewes pregnant and giving birth compared to FG (10 vs. 9 and 9 vs. 6, respectively). Meanwhile, the average value of the BUN content of ewes in both FG and SI treatments during the premating, pregnancy, and lactation phases did not show any differences (p>0.05). The concentration of BUN is still in normal condition even though it has decreased from pre-mating until the lactation phase. It can be concluded that Sorghum-Indigofera mixed feed could increase reproductive success. However, the average value of BUN in the two types of feed treatment experienced the same status change in all reproductive phases. Both can overcome the energy deficit problem if they have sufficient feed

    Appropriate Primer Selection Improves Molecular Bird Sexing Accuracy

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    Birds sexing utilize the Polymerase Chain Reaction (PCR) technique is increasingly being used by researchers and breeders. The PCR technique has high sensitivity, but its success is influenced by the specificity of the DNA template with the oligo primer used. This study aimed to evaluate 5 types of PCR primers P2/P8, 2550F/2718R, CHD1F/CHD1R, 1237L/1272H, and CHD1LF/CHD1LR to determine the sex of Phasianidae, Anatidae, Muscicapidae, and Psittacidae families. This research was conducted by tested primers mentioned above to amplify the target gene chromodomain helicase DNA binding 1 (CHD1) on DNA samples of each pair of males and females from four bird families, respectively. The results indicated that CHD1LF/CHD1LR PCR primer gave the best results and was recommended to determine the sex of four families tested. Some of other primers tested in this study failed to amplify targeted gene correctly, it is important to use appropriate primer to increase bird sexing accuracy

    Analysis of Motility Characteristic of Pesisir Bulls Sexed Semen with Different Pre-Freezing Method Based on Computer Assisted Sperm Analyzer (CASA)

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    This study aimed to find  the best combination between pre-freezing method and layer of post sexing on semen quality of Pesisir Bulls. This study used 2 Pesisir Bulls. The semen was evaluated using Computer Assisted Sperm Analyzer (CASA). This study used factorial randomized block design with 2 factors. Factor A was sexed semen layer of BSA column with 2 level, whereas Factor B was pre-freezing method with 3 level. Analysis of Variance (ANOVA) were used in this study for data Analysis. According to the analysis of variance, there is no interaction of the combination of two factors on each paramters. Pre-freezing method showed highly statistically significant effect (P0,05) on every factor. According to the study, it can be concluded that the best treatment was treatment modified procedure layer by space the straw 16 cm every layers and The quality of post sexing X- and Y- spermatozoa motility effect on motility characteristic such as DCL, DAP, DSL, VCL, VAP, VSL, STR, WOB, ALH and BCF


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