412 research outputs found
Fabrication and Mechanical Evaluation of Anatomically-Inspired Quasilaminate Hydrogel Structures with Layer-Specific Formulations
A major tissue engineering challenge is the creation
of multilaminate scaffolds with layer-specific mechanical
properties representative of native tissues, such as heart valve
leaflets, blood vessels, and cartilage. For this purpose,
poly(ethylene glycol) diacrylate (PEGDA) hydrogels are
attractive materials due to their tunable mechanical and
biological properties. This study explored the fabrication of
trilayer hydrogel quasilaminates. A novel sandwich method
was devised to create quasilaminates with layers of varying
stiffnesses. The trilayer structure was comprised of two
‘‘stiff’’ outer layers and one ‘‘soft’’ inner layer. Tensile testing
of bilayer quasilaminates demonstrated that these scaffolds
do not fail at the interface. Flexural testing showed that the
bending modulus of acellular quasilaminates fell between the
bending moduli of the ‘‘stiff’’ and ‘‘soft’’ hydrogel layers.
The bending modulus and swelling of trilayer scaffolds with
the same formulations were not significantly different than
single layer gels of the same formulation. The encapsulation
of cells and the addition of phenol red within the hydrogel
layers decreased bending modulus of the trilayer scaffolds.
The data presented demonstrates that this fabrication method
can make quasilaminates with robust interfaces, integrating
layers of different mechanical properties and biofunctionalization,
and thus forming the foundation for a multilaminate
scaffold that more accurately represents native tissue
Full quantum reconstruction of vortex states
We propose a complete tomographic reconstruction of any vortex state carrying
orbital angular momentum. The scheme determines the angular probability
distribution of the state at different times under free evolution. To represent
the quantum state we introduce a bona fide Wigner function defined on the
discrete cylinder, which is the natural phase space for the pair angle-angular
momentum. The feasibility of the proposal is addressed.Comment: Final published versio
Recent advances in exciton based quantum information processing in quantum dot nanostructures
Recent experimental developments in the field of semiconductor quantum dot
spectroscopy will be discussed. First we report about single quantum dot
exciton two-level systems and their coherent properties in terms of single
qubit manipulations. In the second part we report on coherent quantum coupling
in a prototype "two-qubit" system consisting of a vertically stacked pair of
quantum dots. The interaction can be tuned in such quantum dot molecule devices
using an applied voltage as external parameter.Comment: 37 pages, 15 figures, submitted to New Journal of Physics, focus
issue on Solid State Quantum Information, added reference
Spitzer/IRAC view of Sh 2-284: Searching for evidence of triggered star formation in an isolated region in the outer Milky Way
Using Spitzer/IRAC observations of a region to be observed by the CoRoT
satellite, we have unraveled a new complex star-forming region at low
metallicity in the outer Galaxy. We perform a study of S284 in order to outline
the chain of events in this star-forming region. We used four-band Spitzer/IRAC
photometry as well as Halpha imaging obtained with INT/WFC. Combining these
data with the optical photometry obtained in the frame of CoRoTs preparation
and the 2MASS catalog we analysed the properties and distribution of young
stellar objects (YSOs) associated with point-like sources. We also studied the
SEDs of regions of extended emission, complementing our dataset with IRAS and
MSX data. We find that S284 is unique in several ways: it is very isolated at
the end of a spiral arm and both the diffuse dust and ionized emission are
remarkably symmetric. We have partially resolved the central clusters of the
three bubbles present in this region. Despite the different scales present in
its multiple-bubble morphology, our study points to a very narrow spread of
ages among the powering high-mass clusters. In contrast, the particular
sawtooth structure of the extended emission at the rim of each ionized bubble
harbours either small lower-mass clusters with a younger stellar population or
individual young reddened protostars. In particular, triggered star formation
is considered to be at work in these regions.Comment: Accepted by A&A. 13 pages, 10 figures, 2 Table
Faraday rotation spectra of bismuth-substituted ferrite garnet films with in-plane magnetization
Single crystalline films of bismuth-substituted ferrite garnets have been
synthesized by the liquid phase epitaxy method where GGG substrates are dipped
into the flux. The growth parameters are controlled to obtain films with
in-plane magnetization and virtually no domain activity, which makes them
excellently suited for magnetooptic imaging. The Faraday rotation spectra were
measured across the visible range of wavelengths. To interprete the spectra we
present a simple model based on the existence of two optical transitions of
diamagnetic character, one tetrahedral and one octahedral. We find excellent
agreement between the model and our experimental results for photon energies
between 1.77 and 2.53 eV, corresponding to wavelengths between 700 and 490 nm.
It is shown that the Faraday rotation changes significantly with the amount of
substituted gallium and bismuth. Furthermore, the experimental results suggest
that the magnetooptic response changes linearly with the bismuth substitution.Comment: 15 pages, 6 figures, published in Phys. Rev.
Elite Suppressor–Derived HIV-1 Envelope Glycoproteins Exhibit Reduced Entry Efficiency and Kinetics
Elite suppressors (ES) are a rare subset of HIV-1–infected individuals who are able to maintain HIV-1 viral loads below the limit of detection by ultra-sensitive clinical assays in the absence of antiretroviral therapy. Mechanism(s) responsible for this elite control are poorly understood but likely involve both host and viral factors. This study assesses ES plasma-derived envelope glycoprotein (env) fitness as a function of entry efficiency as a possible contributor to viral suppression. Fitness of virus entry was first evaluated using a novel inducible cell line with controlled surface expression levels of CD4 (receptor) and CCR5 (co-receptor). In the context of physiologic CCR5 and CD4 surface densities, ES envs exhibited significantly decreased entry efficiency relative to chronically infected viremic progressors. ES envs also demonstrated slow entry kinetics indicating the presence of virus with reduced entry fitness. Overall, ES env clones were less efficient at mediating entry than chronic progressor envs. Interestingly, acute infection envs exhibited an intermediate phenotypic pattern not distinctly different from ES or chronic progressor envs. These results imply that lower env fitness may be established early and may directly contribute to viral suppression in ES individuals
Identifying Canadian Freshwater Fishes through DNA Barcodes
BACKGROUND: DNA barcoding aims to provide an efficient method for species-level identifications using an array of species specific molecular tags derived from the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene. The efficiency of the method hinges on the degree of sequence divergence among species and species-level identifications are relatively straightforward when the average genetic distance among individuals within a species does not exceed the average genetic distance between sister species. Fishes constitute a highly diverse group of vertebrates that exhibit deep phenotypic changes during development. In this context, the identification of fish species is challenging and DNA barcoding provide new perspectives in ecology and systematics of fishes. Here we examined the degree to which DNA barcoding discriminate freshwater fish species from the well-known Canadian fauna, which currently encompasses nearly 200 species, some which are of high economic value like salmons and sturgeons. METHODOLOGY/PRINCIPAL FINDINGS: We bi-directionally sequenced the standard 652 bp "barcode" region of COI for 1360 individuals belonging to 190 of the 203 Canadian freshwater fish species (95%). Most species were represented by multiple individuals (7.6 on average), the majority of which were retained as voucher specimens. The average genetic distance was 27 fold higher between species than within species, as K2P distance estimates averaged 8.3% among congeners and only 0.3% among concpecifics. However, shared polymorphism between sister-species was detected in 15 species (8% of the cases). The distribution of K2P distance between individuals and species overlapped and identifications were only possible to species group using DNA barcodes in these cases. Conversely, deep hidden genetic divergence was revealed within two species, suggesting the presence of cryptic species. CONCLUSIONS/SIGNIFICANCE: The present study evidenced that freshwater fish species can be efficiently identified through the use of DNA barcoding, especially the species complex of small-sized species, and that the present COI library can be used for subsequent applications in ecology and systematics
An Analysis of the Abstracts Presented at the Annual Meetings of the Society for Neuroscience from 2001 to 2006
Annual meeting abstracts published by scientific societies often contain rich arrays of information that can be computationally mined and distilled to elucidate the state and dynamics of the subject field. We extracted and processed abstract data from the Society for Neuroscience (SFN) annual meeting abstracts during the period 2001–2006 in order to gain an objective view of contemporary neuroscience. An important first step in the process was the application of data cleaning and disambiguation methods to construct a unified database, since the data were too noisy to be of full utility in the raw form initially available. Using natural language processing, text mining, and other data analysis techniques, we then examined the demographics and structure of the scientific collaboration network, the dynamics of the field over time, major research trends, and the structure of the sources of research funding. Some interesting findings include a high geographical concentration of neuroscience research in the north eastern United States, a surprisingly large transient population (66% of the authors appear in only one out of the six studied years), the central role played by the study of neurodegenerative disorders in the neuroscience community, and an apparent growth of behavioral/systems neuroscience with a corresponding shrinkage of cellular/molecular neuroscience over the six year period. The results from this work will prove useful for scientists, policy makers, and funding agencies seeking to gain a complete and unbiased picture of the community structure and body of knowledge encapsulated by a specific scientific domain
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Mixed emotions: The contribution of alexithymia to the emotional symptoms of autism
It is widely accepted that autism is associated with disordered emotion processing, and in particular, with deficits of emotional reciprocity such as impaired emotion recognition and reduced empathy. However, a close examination of the literature reveals wide heterogeneity within the autistic population with respect to emotional competence. Here we argue that, where observed, emotional impairments are due to alexithymia - a condition that frequently co-occurs with autism - rather than a feature of autism per se. Alexithymia is a condition characterized by a reduced ability to identify and describe one’s own emotion, but which results in reduced empathy and an impaired ability to recognize the emotions of others. We briefly review studies of emotion processing in alexithymia, and in autism, before describing a recent series of studies directly testing this ‘alexithymia hypothesis’. If found to be correct, the alexithymia hypothesis has wide-reaching implications for the study of autism, and how we might best support sub-groups of autistic individuals with, and without, accompanying alexithymia. Finally, we note the presence of elevated rates of alexithymia, and inconsistent reports of emotional impairments, in eating disorders, schizophrenia, substance abuse, Parkinson’s Disease, Multiple Sclerosis, and anxiety disorders. We speculate that examining the contribution of alexithymia to the emotional symptoms of these disorders may bear fruit
in the same way that it is starting to do in autism
The Brain Atlas Concordance Problem: Quantitative Comparison of Anatomical Parcellations
Many neuroscientific reports reference discrete macro-anatomical regions of the brain which were delineated according to a brain atlas or parcellation protocol. Currently, however, no widely accepted standards exist for partitioning the cortex and subcortical structures, or for assigning labels to the resulting regions, and many procedures are being actively used. Previous attempts to reconcile neuroanatomical nomenclatures have been largely qualitative, focusing on the development of thesauri or simple semantic mappings between terms. Here we take a fundamentally different approach, discounting the names of regions and instead comparing their definitions as spatial entities in an effort to provide more precise quantitative mappings between anatomical entities as defined by different atlases. We develop an analytical framework for studying this brain atlas concordance problem, and apply these methods in a comparison of eight diverse labeling methods used by the neuroimaging community. These analyses result in conditional probabilities that enable mapping between regions across atlases, which also form the input to graph-based methods for extracting higher-order relationships between sets of regions and to procedures for assessing the global similarity between different parcellations of the same brain. At a global scale, the overall results demonstrate a considerable lack of concordance between available parcellation schemes, falling within chance levels for some atlas pairs. At a finer level, this study reveals spatial relationships between sets of defined regions that are not obviously apparent; these are of high potential interest to researchers faced with the challenge of comparing results that were based on these different anatomical models, particularly when coordinate-based data are not available. The complexity of the spatial overlap patterns revealed points to problems for attempts to reconcile anatomical parcellations and nomenclatures using strictly qualitative and/or categorical methods. Detailed results from this study are made available via an interactive web site at http://obart.info
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