Life-Cycle Building Carbon Emission Management Platform based on Building Information Modeling Technology

Buildings produce 40% of annual carbon emissions among various sectors in modern society. One of the most challenging problems of carbon management is how to monitor and calculate a building’s life-cycle energy consumption and carbon emission data during both construction and operation stages. The Building Information Modeling (BIM) technology provides a promising method to obtain and simulate buildings as-is status at different stages in the life cycle. This paper develops a framework for building a carbon emission management platform using the carbon emission factor method and BIM technology, which can derive corresponding carbon emission and measure carbon footprint with building geographic information to achieve precise positioning of carbon emission objects. The platform can achieve multi-role collaboration, equipment visualization, real-time carbon emission monitoring, and data analysis. The platform is applied to an existing building in Hohai University to assess the total carbon footprint of the building in its life cycle. This platform can greatly improve the calculation accuracy of the carbon footprint of buildings, improve data transparency, provide valuable information for building facility management personnel, and help achieve the goal of carbon neutrality

Delineation of early brain development from fetuses to infants with diffusion MRI and beyond

International audienceDynamic macrostructural and microstructural changes take place from the mid-fetal stage to 2 years after birth. Delineating structural changes of the brain during early development provides new insights into the complicated processes of both typical development and the pathological mechanisms underlying various psychiatric and neurological disorders including autism, attention deficit hyperactivity disorder and schizophrenia. Decades of histological studies have identified strong spatial and functional maturation gradients in human gray and white matter. The recent improvements in magnetic resonance imaging (MRI) techniques, especially diffusion MRI (dMRI), relaxometry imaging, and magnetization transfer imaging (MTI) have provided unprecedented opportunities to non-invasively quantify and map the early developmental changes at whole brain and regional levels. Here, we review the recent advances in understanding early brain structural development during the second half of gestation and the first two postnatal years using modern MR techniques. Specifically, we review studies that delineate the emergence and microstructural maturation of white matter tracts, as well as dynamic mapping of inhomogeneous cortical microstructural organization unique to fetuses and infants. These imaging studies converge into maturational curves of MRI measurements that are distinctive across different white matter tracts and cortical regions. Furthermore, contemporary models offering biophysical interpretations of the dMRI-derived measurements are illustrated to infer the underlying microstructural changes. Collectively, this review summarizes findings that contribute to charting spatiotemporally heterogeneous gray and white matter structural development, offering MRI-based biomarkers of typical brain development and setting the stage for understanding aberrant brain development in neurodevelopmental disorders

Advancements in electrochemical biosensing for respiratory virus detection: A review

International audienc

Impact of Insoluble Dietary Fiber and CaCl₂ on Structural Properties of Soybean Protein Isolate–Wheat Gluten Composite Gel

The effect and mechanism of soybean insoluble dietary fiber (SIDF) (0~4%) and CaCl2 (0~0.005 M) on the properties of soybean protein isolate (SPI)–wheat gluten (WG) composite gel were studied. It was revealed that the addition of insoluble dietary fiber (1~2%) increased the strength and water-holding capacity (WHC) of the composite gel (p 2 can increase the critical content of gel texture destruction caused by SIDF, and the gel strength attained its peak at 3% SIDF, indicating that appropriate CaCl2 improved gel structure weakening caused by excessive SIDF. This study provides insights in enhancing the production of multi-component composite gel systems

Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme

microRNAs (miRNAs) have been a class of promising disease diagnostic biomarkers and therapeutic targets for their important biological functions. However, because of the high homology, interference from precursors (pri-miRNA, pre-miRNA), as well as limitations in the current assay technologies, it poses high demand and challenge for a specific, efficient, and economic miRNA assay method. Here, we propose a new miRNA detection method based on a label-free probe and a small organic dye with sequence dependence, realizing the sequence-specific and colorimetric detection of target miRNA. What is pleasantly surprising, only one enzyme is enough to propel the whole miRNA assay process, greatly simplifying the reaction component and detection process. Together with PCR amplification for the high enough sensitivity a nd three checks for specificity control, a detection limit of 5 fM was obtained and even one mutation could be discriminated visually. Overall, the new method makes much progress in convenience and economy of PCR-based miRNA assay method so that miRNA assay is going to be more friendly and affordable

Transition Metal-Mediated CO and CC Bond-Forming Reactions: A Regioselective Strategy for the Synthesis of Imidazo[1,2‑<i>a</i>]pyridines and Imidazo[1,2‑<i>a</i>]pyrazines

A novel and convenient transformation for the regiospecific synthesis of functionalized imidazo­[1,2-<i>a</i>]­pyridine aldehydes/ketones and 3-vinyl imidazo­[1,2-<i>a</i>]­pyridines has been developed via copper­(I)- and palladium­(II)-catalyzed cyclization. The one-pot reaction proceeds smoothly with commercially available catalysts and affords the products in moderate to good yields. It represents an efficient approach for the formation of C–N, CO, and CC bonds under mild conditions

Trans-acting aptazyme for conditional gene knockdown in eukaryotic cells

Trans-acting hammerhead ribozyme inherits the advantages of being the smallest and best-characterized RNA-cleaving ribozyme, offering high modularity and the ability to cleave any desired sequence without the aid of any protein, as long as the target sequence contains a cleavage site. However, achieving precise control over the trans-acting hammerhead ribozyme would enable safer and more accurate regulation of gene expression. Herein, we described an intracellular selection of hammerhead aptazyme that contains a theophylline aptamer on stem II based on toxin protein IbsC. Based on the intracellular selection, we obtained three new cis-acting hammerhead aptazymes. Moreover, the corresponding trans-acting aptazymes could be efficiently induced by theophylline to knock down different targeted genes in eukaryotic cells. Notably, the best one, T195, exhibited a ligand-dependent and dose-dependent response to theophylline, and the cleavage efficiency could be enhanced by incorporating multiplex aptazymes

Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme

microRNAs (miRNAs) have been a class of promising disease diagnostic biomarkers and therapeutic targets for their important biological functions. However, because of the high homology, interference from precursors (pri-miRNA, pre-miRNA), as well as limitations in the current assay technologies, it poses high demand and challenge for a specific, efficient, and economic miRNA assay method. Here, we propose a new miRNA detection method based on a label-free probe and a small organic dye with sequence dependence, realizing the sequence-specific and colorimetric detection of target miRNA. What is pleasantly surprising, only one enzyme is enough to propel the whole miRNA assay process, greatly simplifying the reaction component and detection process. Together with PCR amplification for the high enough sensitivity and three checks for specificity control, a detection limit of 5 fM was obtained and even one mutation could be discriminated visually. Overall, the new method makes much progress in convenience and economy of PCR-based miRNA assay method so that miRNA assay is going to be more friendly and affordable