83 research outputs found
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Syllabus: Conservation of Nature and Culture
This course explores the history of various efforts to conserve nature and culture. Students will learn about the history of environmental conservation, but also to think broadly about what the idea of conservation means in archaeology, historic preservation, and the arts, especially in a time of globalization and climate change. Its fundamental premise is that nature and culture are interconnected; nature cannot truly be conserved without also conserving the culture that has shaped it, and culture cannot truly be conserved without also conserving the natural world on which it rests
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History & Sustainability
Americans debate whether their ever-rising consumption of natural resources and standard of living can continue indefinitely into the future. This is not a new question; since the mid-1800s, movements for the conservation of nature have challenged the primacy of unbridled development and met fierce opposition from those charging that these movements threaten the American dream of individual economic opportunity. Through exploring the history of these ideas, students will gain a better understanding of the meaning of sustainability in contemporary America, especially in response to the forces of global capitalism and the challenges of a changing climate. This course grows out of an earlier course called the Conservation of Nature and Culture. Like that course, its fundamental premise is that nature and culture are interconnected, and that movements for the sustainable use of nat
Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus
Single-stranded DNA-binding protein(SSB)is a well characterized ubiquitous and essential bacterial
protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated areagentless SSB biosensor that can be used as a helicase probe in B. subtilis and closely related gram positive bacteria. We have demonstrated the utility of the probe in a DNA unwinding reaction using a helicase from Bacillus and for the first time,characterized the B. subtilis SSB's DNA binding mode switching and stoichiometry.The importance of SSB in DNA metabolism is not limited to simply binding and protecting ssDNA during DNA replication, as previously thought. It interacts with an array of partner proteins to coordinate many different aspects of DNA metabolism. In most cases its interactions with partner proteins is species-specific and for this reason, knowing how to produce and use cognate reagentless SSB biosensors indifferent bacteria is critical.Here we explain how to produce a B. subtilis SSB probe that exhibits 9-fold fluorescence increase upon binding to single stranded DNA and can be used in all related grampositive firmicutes which employ drastically different DNA replication
and repair systems than the widely studied Escherichiacoli. The materials to produce the B. subtilis SSB probe a recommercially available, so the methodology described here is widely available unlike previously published methods for the E. coli SSB
Grupo ComunitĂĄrio de Porto Alegre - Leigos para o Desenvolvimento
Comunicação apresentada no 3Âș Encontro Conhecimento e Cooperação, INA, Lisboa, 17 de setembro de 201
Beyond the Butter Churn: History, Food, and Social Activism
The current enthusiasm for ââlocal foodââ offers public historians an opportunity to strengthen civic dialogues about place, land and energy use, labor, economy, health, and governance. Join the discussion about how public historians can move beyond conventional exhibits and living history to reshape both scaled-down food systems and civically engaged museums and historic sites.
Moderator: David Glassberg, Department of History, University of Massachusetts Amherst
Presenter: Cathy Stanton, Senior Lecturer, Tufts Universit
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People and Places on the Outer Cape: A Landscape Character Study [2005 EDRA/Places Award -- Research]
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Characterization of Endothelin Receptor Subtypes on Airway Smooth Muscle Cells
Endothelin-1 (ET-1) has constrictor and mitogenic effects on airway smooth muscle strips and cultured cells, respectively. This study addresses the type of the ET receptor subtype(s) present on ovine airway smooth muscle cells and the possibility of autocrine effects. The expression of the preproendothelin-1 gene was demonstrated by Northern analysis, and the medium obtained from these cells contained immunoreactive-ET-1. Competitive binding experiments between [125I]ET-1 and ET-1, ET-3, and two ET-receptor subtype selective-ligands, BQ-123 (ETA and sarafotoxin S6c (ETB), yielded IC50 values of 1.1 ± 0.1, 227 ± 13, 12 ± 1, and 194 ± 21 nM, respectively. ET-3 also gave a limited number of higher affinity sites. In the presence of BQ-123 (1 Ό;M), the binding of [1251]ET-1 was decreased by 80-85%, and the IC50 values with ET-1, ET-3, and S6c were 2.0 ± 0.4, 3.6 ± 0.6, and 1.1 ± 0.9 nM, respectively. In similar experiments with 0.1 ΌM sarafotoxin S6c, the respective IC50 values for ET-1 and ET-3 were 2.4 ± 0.4 and 300 ± 20 nM. These results demonstrate that about 85 ± 5% of ET-1 binding to airway smooth muscle cells is to ETA receptors and that these cells produce ET-1 in vitro
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A transformed murine Leydig cell line expresses the ETA receptor subtype
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