Disease and pest management in apple: Farmers' perception and adoption in J&K state

Diseases and pests are one of the limiting factors for low productivity of the fruit crops in Kashmir valley, India. A study on management of resources with respect to disease and pest management of apple and extent of adoption of recommended plant protection technology was undertaken for increasing apple production in Kashmir valley of J and K State. District Baramulla was selected purposively on the basis of maximum area and production under apple crop. A sample size of 200 apple growers 50 each from 4 villages were selected randomly. The study revealed that the perception index regarding attributes of technology recommended in two diseases viz. San Jose Scale and Apple Scab was 68.88% and 80.76% in respect of profitability (83.97%), simplicity-complexity each 63.57% and 54.27 % for practicability attributes of technology. The data further showed that the farmers adoption level under Chemical control was high at silver tip to green tip stage (80%) and fruit let pea size stage (78%) and medium adoption was observed at pink bloom (bud) stage (74%), petal fall stage (74%) walnut size apple stage (70%) on Apple Scab similarly, the extent of adoption was low (45%) for mechanical and no chemical control measures under clean cultivation. In case of San Jose Scale the farmers adoption level regarding name of chemical, its dose, quantity of water required per acre for preparing spray solution and time of spray at late dormant spray, (feb, March) was high (80%). The findings will help to improve the level of farmers’ knowledge to increase apple production in Kashmir valley

Genetic characterization of Barbari goats using microsatellite markers

Genetic variation in Barbari goats, a highly prolific breed distributed widely in the northern part of India, known for better milk and meat quality, was studied as a part of genetic characterization and conservation. The genomic DNA from 50 unrelated Barbari goats were amplified via PCR with a panel of 21 microsatellite markers, and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining. The number of alleles ranged from 4 to 11, with allele sizes ranging from 88 to 220 bp. The distribution of allele frequencies was between 0.0104 and 0.5208. Polymorphism information content varied from 0.5563 to 0.8348. The population was not in Hardy-Weinberg equilibrium for all except two microsatellite loci (ILSTS044 and ILSTS060). The observed heterozygosity ranged from 0.8478 to 1.0000 while the expected heterozygosity ranged from 0.6208 to 0.8509. Based on the results of the present study, there is a good scope for exploiting the genetic variability in the Barbari goats for further improvement of performance

Detection of mecA gene of methicillin resistant Staphylococcus aureus by PCR assay from raw milk

The occurrence of Staphylococcus aureus and MRSA in foods of animal origin, pose a serious threat to the well- being of humans due to innumerable clinical implications. There is a potential risk of transmission of S. aureus and methicillin-resistant Staphylococcus aureus transmission to humans through raw milk if consumed without maintaining adequate hygienic standards. This study was conducted to assess the prevalence of S. aureus and MRSA from raw milk samples vis-à-vis their phenotypic and genotypic characterization for antimicrobial resistance pattern and presence of mecA gene in raw milk samples of cattle, buffalo and goat in the Jammu city of Jammu and Kashmir. Samples (60) were subjected to California mastitis test to check for their mastitic status. The organisms were cultured and identified on the basis of their cultural, morphological, staining and various biochemical characteristics. The amplification of the mecA gene generated a product with a band size of 533bp upon agarose gel electrophoresis. The S. aureus prevalence was 60, 52 and 60% in raw milk of cattle, buffalo and goat, respectively. Out of 34 S. aureus isolates, 44.1% were MRSA positive

Physics Potential of the ICAL detector at the India-based Neutrino Observatory (INO)

The upcoming 50 kt magnetized iron calorimeter (ICAL) detector at the India-based Neutrino Observatory (INO) is designed to study the atmospheric neutrinos and antineutrinos separately over a wide range of energies and path lengths. The primary focus of this experiment is to explore the Earth matter effects by observing the energy and zenith angle dependence of the atmospheric neutrinos in the multi-GeV range. This study will be crucial to address some of the outstanding issues in neutrino oscillation physics, including the fundamental issue of neutrino mass hierarchy. In this document, we present the physics potential of the detector as obtained from realistic detector simulations. We describe the simulation framework, the neutrino interactions in the detector, and the expected response of the detector to particles traversing it. The ICAL detector can determine the energy and direction of the muons to a high precision, and in addition, its sensitivity to multi-GeV hadrons increases its physics reach substantially. Its charge identification capability, and hence its ability to distinguish neutrinos from antineutrinos, makes it an efficient detector for determining the neutrino mass hierarchy. In this report, we outline the analyses carried out for the determination of neutrino mass hierarchy and precision measurements of atmospheric neutrino mixing parameters at ICAL, and give the expected physics reach of the detector with 10 years of runtime. We also explore the potential of ICAL for probing new physics scenarios like CPT violation and the presence of magnetic monopoles.Comment: 139 pages, Physics White Paper of the ICAL (INO) Collaboration, Contents identical with the version published in Pramana - J. Physic

Handling Conflicts in Depth-First Search for LTL Tableau to Debug Compliance Based Languages

Providing adequate tools to tackle the problem of inconsistent compliance rules is a critical research topic. This problem is of paramount importance to achieve automatic support for early declarative design and to support evolution of rules in contract-based or service-based systems. In this paper we investigate the problem of extracting temporal unsatisfiable cores in order to detect the inconsistent part of a specification. We extend conflict-driven SAT-solver to provide a new conflict-driven depth-first-search solver for temporal logic. We use this solver to compute LTL unsatisfiable cores without re-exploring the history of the solver.Comment: In Proceedings FLACOS 2011, arXiv:1109.239

Consumption of a soy drink has no effect on cognitive function but may alleviate vasomotor symptoms in post-menopausal women; a randomised trial

Purpose: Cognitive decline is commonly reported during the menopausal transition, with memory and attention being particularly affected. The aim of this study was to investigate the effects of a commercially available soy drink on cognitive function and menopausal symptoms in post-menopausal women. Methods: 101 post-menopausal women, aged 44–63 years, were randomly assigned to consume a volume of soy drink providing a low (10 mg/day; control group), medium (35 mg/day), or high (60 mg/day) dose of isoflavones for 12 weeks. Cognitive function (spatial working memory, spatial span, pattern recognition memory, 5-choice reaction time, and match to sample visual search) was assessed using CANTAB pre- and post-the 12 week intervention. Menopausal symptoms were assessed using Greene’s Climacteric Scale. Results: No significant differences were observed between the groups for any of the cognitive function outcomes measured. Soy drink consumption had no effect on menopausal symptoms overall; however, when women were stratified according to the severity of vasomotor symptoms (VMS) at baseline, women with more severe symptoms at baseline in the medium group had a significant reduction (P = 0.001) in VMS post-intervention (mean change from baseline score: − 2.15 ± 1.73) in comparison to those with less severe VMS (mean change from baseline score: 0.06 ± 1.21). Conclusions: Soy drink consumption had no effect on cognitive function in post-menopausal women. Consumption of ~ 350 ml/day (35 mg IFs) for 12 weeks significantly reduced VMS in those with more severe symptoms at baseline. This finding is clinically relevant as soy drinks may provide an alternative, natural, treatment for alleviating VMS, highly prevalent among western women

Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing

[EN] Mutation rates vary by orders of magnitude across biological systems, being higher for simpler genomes. The simplest known genomes correspond to viroids, subviral plant replicons constituted by circular non-coding RNAs of few hundred bases. Previous work has revealed an extremely high mutation rate for chrysanthemum chlorotic mottle viroid, a chloroplastreplicating viroid. However, whether this is a general feature of viroids remains unclear. Here, we have used high-fidelity ultra-deep sequencing to determine the mutation rate in a common host (eggplant) of two viroids, each representative of one family: the chloroplastic eggplant latent viroid (ELVd, Avsunviroidae) and the nuclear potato spindle tuber viroid (PSTVd, Pospiviroidae). This revealed higher mutation frequencies in ELVd than in PSTVd, as well as marked differences in the types of mutations produced. Rates of spontaneous mutation, quantified in vivo using the lethal mutation method, ranged from 1/1000 to 1/800 for ELVd and from 1/7000 to 1/3800 for PSTVd depending on sequencing run. These results suggest that extremely high mutability is a common feature of chloroplastic viroids, whereas the mutation rates of PSTVd and potentially other nuclear viroids appear significantly lower and closer to those of some RNA viruses.This work was supported by the European Research Council (erc.europa.eu; ERC-2011-StG-281191-VIRMUT to RS), the Spanish Ministerio de Economia y Competitividad (www.mineco.gob.es; BFU2013-41329 grant to RS, BFU2014-56812-P grant to RF, and a predoctoral fellowship to ALC), and the Spanish Junta de Comunidades de Castilla-La Mancha (www.castillalamancha.es;postdoctoral fellowship to CB). 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