Histological changes in the cerebelli of adult wistar rats exposed to cigarette smoke

The different constituents of tobacco smoke have been linked to different diseased conditions. In this work, the histological effects of cigarette smoke on the cerebellum of adult male Wistar rats were studied. Sixteen Wistar rats with mean weight of 153.24 ± 4.12 g were grouped equally into four. The Control Group A was exposed to fresh air, while Groups B, C and D animals were each exposed to smoke from one, two and three sticks of cigarette respectively. Each stick of cigarette was completely consumed within an average duration of 11 minutes. Improvised smoking chambers were constructed and used for the exposure daily, while treatment lasted for 28 days. The animals were thereafter sacrificed by cervical dislocation, the cranium was exposed and the brain gently removed and weighed; the cerebellum was excised, weighed, and fixed in formol calcium, and subsequently processed for histological observation using the Haematoxylin and Eosin staining principle. Loss of weight and reduction in weight gain were noticed in the treatment groups, with corresponding reduction in cerebellar weights, in a dose-dependent pattern. Histology also revealed loss of white matter, reduction in thickness of cell layers and their cellular components. Increasing dosage of cigarette smoke could predispose to progressive compromise in the structural integrity and composition of the cerebellum, and this might result in cerebellar dysfunction.Keywords: Cerebellar histology, cerebellar dysfunction, cigarette smoke, rats

Constraints to exclusive breastfeeding practice among breastfeeding mothers in Southwest Nigeria: implications for scaling up

<p>Abstract</p> <p>Background</p> <p>The practice of exclusive breastfeeding is still low despite the associated benefits. Improving the uptake and appropriating the benefits will require an understanding of breastfeeding as an embodied experience within a social context. This study investigates breastfeeding practices and experiences of nursing mothers and the roles of grandmothers, as well as the work-related constraints affecting nurses in providing quality support for breastfeeding mothers in Southwest Nigeria.</p> <p>Methods</p> <p>Using a concurrent mixed method approach, a structured questionnaire was administered to 200 breastfeeding mothers. In-depth interviews were also held with breastfeeding mothers (11), nurses (10) and a focus group discussion session with grandmothers.</p> <p>Results</p> <p>Breastfeeding was perceived as essential to baby's health. It strengthens the physical and spiritual bond between mothers and their children. Exclusive breastfeeding was considered essential but demanding. Only a small proportion (19%) of the nursing mothers practiced exclusive breastfeeding. The survey showed the major constraints to exclusive breastfeeding to be: the perception that babies continued to be hungry after breastfeeding (29%); maternal health problems (26%); fear of babies becoming addicted to breast milk (26%); pressure from mother-in-law (25%); pains in the breast (25%); and the need to return to work (24%). In addition, the qualitative findings showed that significant others played dual roles with consequences on breastfeeding practices. The desire to practice exclusive breastfeeding was often compromised shortly after child delivery. Poor feeding, inadequate support from husband and conflicting positions from the significant others were dominant constraints. The nurses decried the effects of their workload on providing quality supports for nursing mothers.</p> <p>Conclusion</p> <p>Breastfeeding mothers are faced with multiple challenges as they strive to practice exclusive breastfeeding. Thus, scaling up of exclusive breastfeeding among mothers requires concerted efforts at the macro, meso and micro levels of the Nigerian society.</p

Serum Biochemical Changes Associated With The Digestibility Of Raw And Heat Processed Cajanus cajan Seeds In Rats

The level of anti-nutritive food toxicants in exotic breed of Cajanus cajan L. (pigeon pea) was evaluated in this study using an animal model experiment in which animals were fed with raw and heat processed seeds for 35 days. The proximate composition revealed a high protein content (23.21%±0.08), low crude fat (2.01 ± 0.00), carbohydrate (60.87 ± 1.01), ash (4.27 ± 0.10), moisture (9.89 ± 0.12) and crude fibre (2.12 ± 0.31). Phytochemical screening of water and methanol extracts revealed the presence of toxic antinutritive constituents; alkaloids and saponins. Animal feeding studies rated samples roasted and blanched for optimum times best in terms of protein efficiency ratio (PER) although their values were significantly different (

Garlic Oil and Vitamin E Prevent the Adverse Effects of Lead Acetate and Ethanol Separately as well as in Combination in the Drinking Water of Rats

Daily feeding of drinking water containing lead acetate (160 mg/l) or 10% alcohol by volume or a combination of both to rats for a month produced certain deleterious effects through oxidative stress. Both heavy metal lead and alcohol are capable of doing such damages. The deleterious alterations observed were in the parameters of blood, serum and tissues, viz; Hb, Pb, proteins, lipids, lipid per oxidation, Vitamins C and E levels and enzyme activities of AST, ALT, and catalase. Simultaneous feeding of either of the two antioxidants garlic oil (GO) and vitamin E at equal doses of 100 mg/kg/day, to the rats counteracted the deleterious effects of the above two chemicals significantly. The maximum damage was brought about by feeding of drinking water containing both lead acetate and alcohol. The protective effects of GO and Vitamin E were not significantly different. The mechanism of actions of the Vitamin E and GO is probably due to their efficiency as detoxifying agents and antioxidants, to scavenging free radicals as well as an independent action of GO on the removal of lead salt as lead sulfide

Redox-fibrosis:impact of TGFβ1 on ROS generators, mediators and functional consequences

Abstract Fibrosis is one of the most prevalent features of age-related diseases like obesity, diabetes, non-alcoholic fatty liver disease, chronic kidney disease, or cardiomyopathy and affects millions of people in all countries. Although the understanding about the pathophysiology of fibrosis has improved a lot during the recent years, a number of mechanisms still remain unknown. Although TGF-β1 signaling, loss of metabolic homeostasis and chronic low-grade inflammation appear to play important roles in the pathogenesis of fibrosis, recent evidence indicates that oxidative stress and the antioxidant system may also be crucial for fibrosis development and persistence. These findings point to a concept of a redox-fibrosis where the cellular oxidant and antioxidant system could be potential therapeutic targets. The current review aims to summarize the existing links between TGF-β1 signaling, generation and action of reactive oxygen species, expression of antioxidative enzymes, and functional consequences including epigenetic redox-mediated responses during fibrosis