180 research outputs found
Sustainable management of scab control through the integration of apple resistant cultivars in a low-fungicide input system
Evaluation of the sustainability of disease control strategies through experimental field studies is poorly documented. Plant genetic resistance to pathogens offers an interesting alternative to the use of pesticides, but pathogen populations are able to adapt, thus frequently resulting in the breakdown of the resistance. Partial resistance is considered to provide more durable resistance than major genes. However, partial resistance does not confer complete protection and its efficiency can also decrease. Developing appropriate strategies which integrate resistant cultivars into crop systems is therefore needed to increase the efficiency and durability of the resistance, whatever the kind of resistance. The aim of this study was to evaluate the relevance of the association of control methods in terms of increasing the efficiency and durability of two kinds of resistances: (i) partial resistance in the apple cultivar Reine des Reinettes and (ii) major resistance (Rvi6) in the apple cultivar Ariane, when planted in a region where the climatic conditions are very favourable to the disease. It was found that the removal of leaf litter in autumn together with spraying of fungicides in the case of moderate or high risks of scab infection resulted in a sustainable control of scab on Reine des Reinettes over a five-year period and delayed the breakdown of the major resistance Rvi6 of Ariane by virulent isolates
Sustainable control of apple scab obtained by the association of cultivar resistance, sanitation and soft chemical control
Treatment solutions to cure Xylella fastidiosa diseased plants
This opinion addresses the question of the efficacy of current treatment solutions to cure Xylella fastidiosa diseased plants, and discusses the experimental treatments under evaluation by two research groups in Apulian olive orchards infected by strain CoDiRO (Complesso del Disseccamento Rapido dell'Olivo). The increasing problems from newly emerging vascular bacterial diseases and the limited success to cure plants from such infections have stimulated numerous studies on treatments with chemical and biological compounds. Under field conditions, various formulations of copper and zinc as spray or root drench are currently used while further options, for example the application of bioactive substances, are at an experimental stage. In Apulia, preliminary results from intensive treatments with such formulations, in combination with the use of good crop management practices, reported more vigorous new growth of diseased trees. However, results provided so far confirmed the continued presence of X. fastidiosa after the treatments under evaluation. This is in agreement with current knowledge that there are no means to cure plants from this bacterial disease, in the sense of eliminating the pathogen from plant tissues. The reported positive response of the treated olive trees is most probably due to the effect of micronutrients and other bioactive compounds that, together with soil cultivation and agronomical practices, improve the vigour of the plants and their resilience to stress caused by bacterial infections. Notwithstanding the preliminary status of these findings, the Panel acknowledged the potentially positive effects of such treatments in prolonging the productive phase of olive trees and their putative relevance for the management of olive orchards, particularly in the containment area where eradication of the pathogen is considered no longer possible. The Panel also concluded that long-term studies are needed to confirm that the reported positive effects on crop performance can be sustained over many years
Pest categorisation of Pseudocercospora pini-densiflorae
Following a request from the European Commission, the EFSA Plant Health (PLH) Panel performed a
pest categorisation of Pseudocercospora pini-densiflorae, a well-defined and distinguishable fungal
species of the family Mycosphaerellaceae. The regulated harmful organism is the anamorph
Cercoseptoria pini-densiflorae (synonym Cercospora pini-densiflorae) with the corresponding teleomorph
Mycosphaerella gibsonii. P. pini-densiflorae causes a needle blight of Pinus spp. also known as
Cercospora blight of pines or Cercospora needle blight. P. pini-densiflorae is reported from sub-Saharan
Africa, Central and South America, Asia and Oceania, but not from the EU. The pathogen is regulated in
Council Directive 2000/29/EC (Annex IIAI) as a quarantine organism whose introduction into the EU is
banned on plants (other than fruit and seeds) and wood of Pinus. The pest could enter the EU via plants
for planting and other means (uncleaned seed, cut branches of pine trees, isolated bark, growing media
accompanying plants, and mycorrhizal soil inocula). Hosts are widespread in the EU and favourable
climatic conditions are present in Mediterranean countries. Pinus halepensis, Pinus nigra, Pinus pinea,
Pinus pinaster and Pinus sylvestris are reported to be highly susceptible to the pathogen. The pest would
be able to spread following establishment after introduction in the EU mainly on infected plants for
planting. The pest introduction could have impacts in nurseries and young plantations. Cleaning seeds
from needles and removing infected seedlings and pine litter from affected nurseries can reduce the risk
of establishment in nurseries and of spread from nurseries to forests, especially given the limited scale of
splash dispersal. The main knowledge gaps concern (i) the role of means of entry/spread other than
plants for planting and (ii) the potential consequences in mature tree plantations and forests. The criteria
assessed by the Panel for consideration as potential quarantine pest are met. For regulated nonquarantine
pests, the criterion on the pest presence in the EU is not met
Pest categorisation of Ips cembrae
The Panel on Plant Health performed a pest categorisation of the large larch bark beetle, Ips cembrae
(Heer) (Coleoptera: Curculionidae, Scolytinae), for the EU. I. cembrae is a well-defined and
distinguishable species, native to Europe and recognised mainly as a pest of larch (Larix spp.) and
occasionally of pine (Pinus spp.) and spruce (Picea spp.). It is distributed in 16 Member States of the
EU and listed in Annex IIB of Council Directive 2000/29/EC. Protected zones are in place in Greece,
Ireland and the United Kingdom (Northern Ireland and Isle of Man). Wood, wood products, bark and
wood packaging material are considered as pathways for this pest, which is also able to disperse by
flight. The insects normally establish on fallen or weakened trees but, when their populations are high,
can also mass-attack healthy trees. The males produce aggregation pheromones that attract
conspecifics of both sexes. The insects also inoculate pathogenic fungi to their hosts. There are one to
two generations per year. Before establishing their broods, the young adults need to proceed to
maturation feeding either within the bark of the tree where they developed or in 2–18 years old twigs.
I. cembrae has been expanding its geographical range in Europe during the second half of the 20th
century. Sanitary thinning or clear felling is the major control methods. Quarantine measures are
implemented to prevent entry in the protected zones. All criteria for consideration as potential
protected zone quarantine pest are met. The criteria for considering I. cembrae as a potential
regulated non-quarantine pest are not met since plants for planting are not viewed as a major
pathway
Susceptibility of Phoenix roebelenii to Xylella fastidiosa
Following a request from the European Commission, the EFSA Plant Health Panel analysed a dossier submitted by Costa Rica Authorities to reach a conclusion on the host status of Phoenix roebelenii for Xylella fastidiosa. The Panel wishes first to stress the difficulties faced in providing compelling evidence for the non-susceptibility status of any particular plant species. The Panel acknowledges that the listing of P. roebelenii as a host of X. fastidiosa rests on a single report from California. Because isolation of X. fastidiosa from some hosts can be difficult, the Panel considers that the failure to isolate X. fastidiosa from P. roebelenii cannot be used to totally discard the detection of X. fastidiosa by ELISA and PCR. The Panel concludes that the detection of X. fastidiosa by two independent techniques provides sufficient evidence, although not totally conclusive, for the listing of P. roebelenii as a X. fastidiosa host plant. Concerning the survey data provided in the Costa Rican dossier, the Panel wishes to stress that such surveys cannot demonstrate the non-host status but can only provide a probability bound, upper estimate of the proportion of infected plants in the field. In the present case, and assuming all survey parameters to be optimal, the 95% confidence incidence threshold obtained is 0.2%, leaving the possibility that close to 25,000 P. roebelenii plants could be infected but undetected in the country. Accepting a scenario of local, non-systemic infection of P. roebelenii by X. fastidiosa would further increase uncertainties. In addition, the absence of data on the vector infection pressure further affects the ability to derive meaningful information on the P. roebelenii host status from the survey data. Appropriately conducted mechanical and/or vector-mediated inoculation experiments are critical to reach a more solid conclusion on the X. fastidiosa host status of P. roebelenii
Pest risk assessment of Radopholus similis for the EU territory
The Panel on Plant Health performed a pest risk assessment on Radopholus similis, the burrowing
nematode for the EU. The quantitative assessment focused on entry, establishment, spread and impact
on tropical and subtropical ornamental host plants, the main pathways for entry of R. similis into the
EU. Infested consignments are expected to enter the risk assessment area on ornamentals under all
scenarios. For citrus, which is a closed pathway for entry, outdoor establishment was assessed.
Establishment may only take place after successful transfer from ornamental plants to citrus
production systems. This event is called ‘shift’ in this assessment, to indicate that this is an unusual
transfer. It has been estimated that establishment of this nematode in the open field in the EU citrus
production areas under current temperatures is possible in most parts of the citrus production area in
the EU. Temperature conditions will prevent the nematode from establishing only in the northernmost
citrus areas and at higher altitudes in the south. Host plants for planting originating from infested
places of production (greenhouses) within the risk assessment area are considered the main pathway
for spread within the risk assessment area. Under current climatic conditions, the population of
R. similis is not expected to reach damaging population levels in the open field. In case of increased
temperatures due to global warming, the nematode population may reach damaging levels in very few
places outdoors. Currently, main impact is considered for ornamental greenhouse production in the risk
assessment area. Impact will be either caused by direct plant growth reductions or loss due to
phytosanitary measures applied on regulated plants. Despite the fact that R. similis is globally
considered as one of the most destructive plant parasitic nematodes, the impact in the risk assessment
area is considered low
Citrus junos as a host of citrus bacterial canker
Following a request from the European Commission, the European Food Safety Authority (EFSA) Plant Health (PLH) Panel analysed a dossier submitted by the Japanese authorities in order to clarify the host status of Citrus junos with regard to Xanthomonas citri pv. citri and Xanthomonas citri pv. aurantifolii, causal agents of citrus bacterial canker, and to indicate whether C. junos fruit could represent a pathway for the introduction of citrus bacterial canker into the European Union. In a previous opinion in the year 2014, the EFSA PLH Panel concluded that commercial fresh citrus fruit is generally pathway and that no commercially important Citrus species or variety can be considered as immune to citrus bacterial canker. In the current assessment, the EFSA PLH Panel analysed the two scientific papers provided by the Japanese authorities, as well as 16 additional papers identified through a systematic literature review. The PLH Panel considered that the conclusions of its previous opinion remain valid and that convergent lines of evidence provide sufficient demonstration that C. junos is a host of X. citri pv. citri and X. citri pv. aurantifolii. Therefore, there is no reason to consider the C. junos fruit differently from other citrus species. Consequently, the assessment of the general citrus fruit pathway from the 2014 opinion still applies. Uncertainties on these conclusions are a result of the scarce scientific evidence published on this subject in addition to the methodological and reporting limitations of the published papers
Dynamics of Dynamics within a Single Data Acquisition Session: Variation in Neocortical Alpha Oscillations in Human MEG
Background
Behavioral paradigms applied during human recordings in electro- and magneto- encephalography (EEG and MEG) typically require 1–2 hours of data collection. Over this time scale, the natural fluctuations in brain state or rapid learning effects could impact measured signals, but are seldom analyzed.
Methods and Findings
We investigated within-session dynamics of neocortical alpha (7–14 Hz) rhythms and their allocation with cued-attention using MEG recorded from primary somatosensory neocortex (SI) in humans. We found that there were significant and systematic changes across a single ~1 hour recording session in several dimensions, including increased alpha power, increased differentiation in attention-induced alpha allocation, increased distinction in immediate time-locked post-cue evoked responses in SI to different visual cues, and enhanced power in the immediate cue-locked alpha band frequency response. Further, comparison of two commonly used baseline methods showed that conclusions on the evolution of alpha dynamics across a session were dependent on the normalization method used.
Conclusions
These findings are important not only as they relate to studies of oscillations in SI, they also provide a robust example of the type of dynamic changes in brain measures within a single session that are overlooked in most human brain imaging/recording studies.National Institutes of Health (U.S.) (P41RR14075)National Institutes of Health (U.S.) (K25MH072941)National Institutes of Health (U.S.) (K01AT003459)National Institutes of Health (U.S.) (1RO1-NS045130-01)National Institutes of Health (U.S.) (T32GM007484)National Science Foundation (U.S.) (0316933)Osher Lifelong Learning Institute
Musculoskeletal symptoms of the upper extremities and the neck: A cross-sectional study on prevalence and symptom-predicting factors at visual display terminal (VDT) workstations
<p>Abstract</p> <p>Background</p> <p>The aim of this study was to determine the prevalence and the predictors of musculoskeletal symptoms in the upper extremities and neck at visual display terminal (VDT) workstations.</p> <p>Methods</p> <p>In a cross-sectional study 1,065 employees working at VDT > 1 h/d completed a standardised questionnaire. Workstation conditions were documented in a standardised checklist, and a subgroup of 82 employees underwent a physical examination.</p> <p>Results</p> <p>Using the Nordic Questionnaire, the 12-month prevalence of symptoms of the neck, shoulder region, hand/wrist, or elbow/lower arm was 55%, 38%, 21%, and 15% respectively. The duration of VDT work had a significant impact on the frequency of neck symptoms in employees performing such work > 6 h/d.</p> <p>Conclusion</p> <p>With regard to musculoskeletal symptoms of the upper extremities, preventive measures at VDT workstations should be focused on neck and shoulder symptoms (e.g. ergonomic measures, breaks to avoid sitting over long periods).</p
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