The Effect of Distal Core Flattening and Heat Treatment on 304 Stainless Steel Guide Wires

The mechanical response of 304 stainless steel guide wires due to different temper conditions and amounts of flattening is to be explored in this project. For this specific project, there is no public literature on the mechanical properties of guide wires with the above conditions through tensile testing or Turns to Failure Testing. To address this, the project with Abbott Vascular will measure the mechanical properties of guide wires using the aforementioned factors. Due to a lack of previous literature on this research topic, there are no quantitative goals for the project, however, any new research compiled in this area will be beneficial. As a result of the COVID-19 pandemic, current limitations to lab access prevent new data from being collected on guide wires, so this report will mainly focus on existing data from wire manufacturers. Available tensile properties of 304 stainless steel at varying cold work levels will be taken into account to better understand guide wire behavior and allow us to suggest the next steps in order to progress this project in the future

Divergent responses to peptidoglycans derived from different E. coli serotypes influence inflammatory outcome in trout, Oncorhynchus mykiss, macrophages

Background: Pathogen-associated molecular patterns (PAMPs) are structural components of pathogens such as lipopolysaccharide (LPS) and peptidoglycan (PGN) from bacterial cell walls. PAMP-recognition by the host results in an induction of defence-related genes and often the generation of an inflammatory response. We evaluated both the transcriptomic and inflammatory response in trout (O. mykiss) macrophages in primary cell culture stimulated with DAP-PGN (DAP; meso-diaminopimelic acid, PGN; peptidoglycan) from two strains of Escherichia coli (PGN-K12 and PGN-O111:B4) over time. Results: Transcript profiling was assessed using function-targeted cDNA microarray hybridisation (n = 36) and results show differential responses to both PGNs that are both time and treatment dependent. Wild type E. coli (K12) generated an increase in transcript number/diversity over time whereas PGN-O111:B4 stimulation resulted in a more specific and intense response. In line with this, Gene Ontology analysis (GO) highlights a specific transcriptomic remodelling for PGN-O111:B4 whereas results obtained for PGN-K12 show a high similarity to a generalised inflammatory priming response where multiple functional classes are related to ribosome biogenesis or cellular metabolism. Prostaglandin release was induced by both PGNs and macrophages were significantly more sensitive to PGN-O111:B4 as suggested from microarray data. Conclusion: Responses at the level of the transcriptome and the inflammatory outcome (prostaglandin synthesis) highlight the different sensitivity of the macrophage to slight differences (serotype) in peptidoglycan structure. Such divergent responses are likely to involve differential receptor sensitivity to ligands or indeed different receptor types. Such changes in biological response will likely reflect upon pathogenicity of certain serotypes and the development of disease

Influence of environmental factors on surface activity of \u3cem\u3eParuroctonus marksi\u3c/em\u3e (Scorpiones: Vaejovidae) in the Mojave Desert

Many environmental factors may influence the activity pattern of scorpions in arid ecosystems. We investigated the seasonal variations of Paruroctonus marksi scorpion activity in the Mojave Desert (California, USA). Black light surveys were carried out within two 100 ×100 m plots from August 2017 to September 2018. The majority (85%) of scorpions were found in open areas compared with being in vegetation. The presence of P. marksi was positively correlated with both water vapor pressure and soil temperature, while moon illumination had no significant effect on P. marksi foraging behavior. Scorpions might be using humidity as an indicator of prey abundance and since they are not visual hunters, and moonlight does not affect their surface activity possibly due to lack of visual hunters in the study site

Chloroplast phylogenomic analyses reveal the deepest-branching lineage of the Chlorophyta, Palmophyllophyceae class. nov.

The green plants (Viridiplantae) are an ancient group of eukaryotes comprising two main clades: the Chlorophyta, which includes a wide diversity of green algae, and the Streptophyta, which consists of freshwater green algae and the land plants. The early-diverging lineages of the Viridiplantae comprise unicellular algae, and multicellularity has evolved independently in the two clades. Recent molecular data have revealed an unrecognized early-diverging lineage of green plants, the Palmophyllales, with a unique form of multicellularity, and typically found in deep water. The phylogenetic position of this enigmatic group, however, remained uncertain. Here we elucidate the evolutionary affinity of the Palmophyllales using chloroplast genomic, and nuclear rDNA data. Phylogenetic analyses firmly place the palmophyllalean Verdigellas peltata along with species of Prasinococcales (prasinophyte clade VI) in the deepest-branching clade of the Chlorophyta. The small, compact and intronless chloroplast genome (cpDNA) of V. peltata shows striking similarities in gene content and organization with the cpDNAs of Prasinococcales and the streptophyte Mesostigma viride, indicating that cpDNA architecture has been extremely well conserved in these deep-branching lineages of green plants. The phylogenetic distinctness of the Palmophyllales-Prasinococcales clade, characterized by unique ultrastructural features, warrants recognition of a new class of green plants, Palmophyllophyceae class. nov

Configuración, puesta en marcha y aplicaciones de NethServer en GNU/Linux

Con este trabajo se da solución a una problemática de nivel corporativo el cual se resolverá de forma práctica mediante el sistema operativo GNU/Linux nethserver poniendo en marcha desde cero y a su vez configurar el sistema de seguridad y de infraestructura de red, también se demostrará todo lo aprendido durante el diplomado todo el conocimiento adquirido en cuanto a implementación, configuración y desarrollo del sistema operativo Linux y demostrar su gran utilidadWith this work, a solution is given to a corporate level problem which will be solved in a practical way through the GNU / Linux nethserver operating system, starting from scratch and in turn configuring the security system and network infrastructure, everything will also be demonstrated. what was learned during the diploma all the knowledge acquired in terms of implementation, configuration and development of the Linux operating system and demonstrate its great utilit

Mitogen-Activated Protein Kinase Phosphatase-1 Is Overexpressed in Non-Small Cell Lung Cancer and Is an Independent Predictor of Outcome in Patients

An increase in the activity of the mitogen-activated protein kinases (MAPKs) has been correlated with a more malignant phenotype in several tumor models in vitro and in vivo. A key regulatory mechanism of the MAPKs [extracellular signal-regulated kinase (ERK); c-jun NH(2)-terminal kinase (JNK); and p38] is the dual specificity phosphatase CL100, also called MAPK phosphatase-1 (MKP-1). This study was designed to examine the involvement of CL100/MKP-1 and stress-related MAPKs in lung cancer. EXPERIMENTAL DESIGN: We assessed the expression of CL100/MKP-1 and the activation of the MAPKs in a panel of 18 human cell lines [1 primary normal bronchial epithelium, 8 non-small cell lung cancer (NSCLC), 7 small cell lung cancer (SCLC), and 2 carcinoids] and in 108 NSCLC surgical specimens. RESULTS: In the cell lines, CL100/MKP-1 expression was substantially higher in NSCLC than in SCLC. P-ERK, P-JNK, and P-p38 were activated in SCLC and NSCLC, but the degree of their activation was variable. Immunohistochemistry in NSCLC resection specimens showed high levels of CL100/MKP-1 and activation of the three MAPK compared with normal lung. In univariate analysis, no relationship was found among CL100/MKP-1 expression and P-ERK, P-JNK, or P-p38. Interestingly, high CL100/MKP-1 expression levels independently predicted improved survival in multivariate analysis. JNK activation associated with T(1-2) and early stage, whereas ERK activation correlated with late stages and higher T and N. Neither JNK nor ERK activation were independent prognostic factors when studied for patient survival. CONCLUSIONS: Our data indicate the relevance of MAPKs and CL100/MKP-1 in lung cancer and point at CL100/MKP-1 as a potential positive prognostic factor in NSCLC. Finally, our study supports the search of new molecular targets for lung cancer therapy within the MAPK signaling pathway

Superconducting spiral bandpass filter designed by a pseudo-Fourier technique

An iterative technique to adjust ripple in a bandpass filter has been developed by curve-fitting numerical data. It gives an approximate relation of ripple against filter coupling coefficients and resonator resonant frequencies instead of finding a Jacobian matrix for each individual filter, in exchange for making more iterations. Numerical examples show reasonable convergence for Chebyshev filters up to the 16th-order, coupled quadruplets, coupled triplets and folded cross-coupled filters. A bandpass filter for a radio telescope in Yebes, Spain is designed and measured, a tenth-order superconducting spiral filter with 7% bandwidth, 2295 MHz centre frequency, <0.1 dB ripple and overall substrate size 30 mm × 8 mm. The 11 iterative simulations totalled 8.5 h of computer processing time