Reading mixtures of uniform sequence-defined macromolecules to increase data storage capacity

In recent years, the field of molecular data storage has emerged from a niche to a vibrant research topic. Herein, we describe a simultaneous and automated read-out of data stored in mixtures of sequence-defined oligomers. Therefore, twelve different sequence-defined tetramers and three hexamers with different mass markers and side chains are successfully synthesised via iterative Passerini three-component reactions and subsequent deprotection steps. By programming a straightforward python script for ESI-MS/MS analysis, it is possible to automatically sequence and thus read-out the information stored in these oligomers within one second. Most importantly, we demonstrate that the use of mass-markers as starting compounds eases MS/MS data interpretation and furthermore allows the unambiguous reading of sequences of mixtures of sequence-defined oligomers. Thus, high data storage capacity considering the field of synthetic macromolecules (up to 64.5 bit in our examples) can be obtained without the need of synthesizing long sequences, but by mixing and simultaneously analysing shorter sequence-defined oligomers

Effects of Intravenous Aspirin on Prostaglandin Synthesis and Kidney Function in Intensive Care Patients

The effects of intravenous acetylsalicylic acid (1.0 g bolus) on renal function and prostaglandin synthesis were evaluated in a prospective, controlled study in eight patients in an intensive care unit. Four of these patients had congestive heart failure. Administration of acetylsalicylic acid caused significant antidiuresis (−56%), antinatriuresis (−82%), renin suppression (−26%) and decreased GFR (−41%). All of these changes were completely reversible within 1-2 hours and tended to be more pronounced in the patients with congestive heart failure. Urinary excretion of prostaglandin E was depressed profoundly (−93%) and did not return to more than 45% of control 6 h after the administration of acetylsalicylic acid. We conclude that intravenous acetylsalicylic acid affects kidney function in a manner similar to other prostaglandin synthesis inhibitors. Its effects are, however, short-lived. The inhibition of urinary PGE2 excretion outlasts GFR depression, antidiuresis, antinatriuresis and renin suppression by several hour

Dual sequence definition increases the data storage capacity of sequence-defined macromolecules

Sequence-defined macromolecules offer applications in the field of data storage. Challenges include synthesising precise and pure sequences, reading stored information and increasing data storage capacity. Herein, the synthesis of dual sequence-defined oligomers and their application for data storage is demonstrated. While applying the well-established Passerini three-component reaction, the degree of definition of the prepared monodisperse macromolecules is improved compared to previous reports by utilising nine specifically designed isocyanide monomers to introduce backbone definition. The monomers are combined with various aldehyde components to synthesise dual-sequence defined oligomers. Thus, the side chains and the backbones of these macromolecules can be varied independently, exhibiting increased molecular diversity and hence data storage capacity per repeat unit. In case of a dual sequence-defined pentamer, 33 bits are achieved in a single molecule. The oligomers are obtained in multigram scale and excellent purity. Sequential read-out by tandem ESI-MS/MS verifies the high data storage capacity of the prepared oligomers per repeat unit in comparison to other sequence defined macromolecules

Many-body effects in nuclear structure

We calculate, for the first time, the state-dependent pairing gap of a finite nucleus (120Sn) diagonalizing the bare nucleon-nucleon potential (Argonne v14) in a Hartree-Fock basis (with effective k-mass m_k eqult to 0.7 m), within the framework of the BCS approximation including scattering states up to 800 MeV above the Fermi energy to achieve convergence. The resulting gap accounts for about half of the experimental gap. We find that a consistent description of the low-energy nuclear spectrum requires, aside from the bare nucleon-nucleon interaction, not only the dressing of single-particle motion through the coupling to the nuclear surface, to give the right density of levels close to the Fermi energy (and thus an effective mass m* approximately equal to m), but also the renormalization of collective vibrational modes through vertex and self-energy processes, processes which are also found to play an essential role in the pairing channel, leading to a long range, state dependent component of the pairing interaction. The combined effect of the bare nucleon-nucleon potential and of the induced pairing interaction arising from the exchange of low-lying surface vibrations between nucleons moving in time reversal states close to the Fermi energy accounts for the experimental gap.Comment: 5 pages, 4 figures; author list correcte

p-Type semiconducting properties in lithium-doped MgO single crystals

The phenomenally large enhancement in conductivity observed when Li-doped MgO crystals are oxidized at elevated temperatures was investigated by dc and ac electrical measurements in the temperature interval 250-673 K. The concentration of ([Li]^{0}) centers (Li^{+} ions each with a trapped hole) resulting from oxidation was monitored by optical absorption measurements. Both dc and ac experiments provide consistent values for the bulk resistance. The electricalconductivity of oxidized MgO:Li crystals increases linearly with the concentration of ([Li]^{0}) centers. The conductivity is thermally activated with an activation energy of (0.70 +/- 0.01) eV, which is independent of the ([Li]^{0}) content. The \textit{standard semiconducting} mechanism satisfactorily explains these results. Free holes are the main contribution to band conduction as they are trapped at or released from the ([Li]^{0})-acceptor centers. In as-grown MgO:Li crystals, electrical current increases dramatically with time due to the formation of ([Li]^{0}) centers. The activation energy values between 1.3 and 0.7 eV are likely a combination of the activation energy for the creation of ([Li]^{0}) centers and the activation energy of ionization of these centers. Destruction of ([Li]^{0}) centers can be induced in oxidized crystals by application of an electric field due to Joule heating up to temperatures at which ([Li]^{0}) centers are not stable.Comment: LaTeX, 20 pages, 9 Encapsulated Postscript Format Figures, use the version 4.0 of REVTEX 4 macro packag

Flow equations for QED in the light front dynamics

The method of flow equations is applied to QED on the light front. Requiring that the partical number conserving terms in the Hamiltonian are considered to be diagonal and the other terms off-diagonal an effective Hamiltonian is obtained which reduces the positronium problem to a two-particle problem, since the particle number violating contributions are eliminated. No infrared divergencies appear. The ultraviolet renormalization can be performed simultaneously.Comment: 15 pages, Latex, 3 pictures, Submitted to Phys.Rev.

Chern - Simons Gauge Field Theory of Two - Dimensional Ferromagnets

A Chern-Simons gauged Nonlinear Schr\"odinger Equation is derived from the continuous Heisenberg model in 2+1 dimensions. The corresponding planar magnets can be analyzed whithin the anyon theory. Thus, we show that static magnetic vortices correspond to the self-dual Chern - Simons solitons and are described by the Liouville equation. The related magnetic topological charge is associated with the electric charge of anyons. Furthermore, vortex - antivortex configurations are described by the sinh-Gordon equation and its conformally invariant extension. Physical consequences of these results are discussed.Comment: 15 pages, Plain TeX, Lecce, June 199

Poincare Semigroup Symmetry as an Emergent Property of Unstable Systems

The notion that elementary systems correspond to irreducible representations of the Poincare group is the starting point for this paper, which then goes on to discuss how a semigroup for the time evolution of unstable states and resonances could emerge from the underlying Poincare symmetry. Important tools in this analysis are the Clebsch-Gordan coefficients for the Poincare group.Comment: 17 pages, 1 figur

The Critical Behaviour of Potts models with symmetry breaking fields

The $Q$-state Potts model in two dimensions in the presence of external magnetic fields is studied. For general $Q\geq3$ special choices of these magnetic fields produce effective models with smaller $Z(Q')$ symmetry $(Q'< Q)$. The phase diagram of these models and their critical behaviour are explored by conventional finite-size scaling and conformal invariance. The possibility of multicritical behavior, for finite values of the symmetry breaking fields, in the cases where $Q>4$ is also analysed. Our results indicate that for effective models with $Z(Q')$ symmetry $(Q'\leq4)$ the multicritical point occurs at zero field. This last result is also corroborated by Monte Carlo simulations.Comment: 15 pages (standart LaTex), 2 figure (PostScript) available by request to [email protected]