3,778 research outputs found
Coral Reefs, Fisheries, and Food Security: Integrated Approaches to Addressing Multiple Challenges in the Coral Triangle
The Coral Triangle is the most biologically and economically valuable marine ecosystem on the planet. Covering just three percent of the globe, the region represents more than half of the world's reefs and boasts 76 percent of its known coral species. Sustaining more than 130 million people who rely directly on the marine ecosystems for their livelihoods and food, the marine habitats of the Coral Triangle contribute billions of dollars each year toward the economies of the region.Although the environmental imperative for preserving this area of incredible value and biodiversity is obvious, the growing pressures and threats from widespread poverty, rapid development, and global demands continue to place enormous strain on the natural marine resources of the Coral Triangle
The lipocalin protein family: A role in cell regulation
AbstractThe lipocalins, a large, diverse, but relatively poorly understood family of small extracellular proteins, are characterized by the ability to bind small hydrophobic molecules, such as retinol, and by their binding to specific cell surface receptors. These general properties suggest such proteins as appropriate transporters transferring biologically hazardous molecules in a safe and controlled manner between cells. Moreover, many lipocalins have been implicated in the regulation of cell homeostasis: apolipoprotein D, quiescience specific protein, purpurin, α-1-microglobulin, and NGAL. This combination of direct and indirect evidence suggests that the lipocalin protein family may be involved, in a quite general way, in the mediation of cell regulation and that many presently functionless family members might act in this way
Loss of hypothermic and anti-pyretic action of paracetamol in cyclooxygenase-1 knockout mice is indicative of inhibition of cyclooxygenase-1 variant enzymes
Paracetamol (acetaminophen), is a centrally-acting antipyretic analgesic drug, which can also lower body temperature. Despite a century of clinical use, its mechanism of pharmacological action has not been completely elucidated. Previously, we demonstrated significant attenuation in the paracetamol induced hypothermia in parallel with its inhibitory action on the synthesis of brain prostaglandin Eâ‚‚ (PGEâ‚‚) in cyclooxygenase-1 (COX-1) knockout mice in comparison to wild-type mice. The above reported pharmacological actions by paracetamol were completely retained in COX-2 knockout mice. We thus concluded that the mechanism of hypothermic action of paracetamol is dependent on inhibition of a COX-1 gene-derived enzyme. In the current investigation, we provide further support for this notion by demonstrating that the paracetamol-induced hypothermia is not mediated through inhibition of COX-1 as neither the COX-1 selective inhibitor, SC560, nor the COX-1/COX-2 dual inhibitor, indomethacin, induced hypothermia at pharmacologically active doses in mice. In addition, using a COX-2-dependent and PGEâ‚‚-mediated model of endotoxin-induced fever, paracetamol induced anti-pyretic and hypothermic actions in COX-1 wild-type mice. These effects were fully or partially attenuated in COX-1 knockout mice after prophylactic or therapeutic administration, respectively. Therapeutically-administered paracetamol also reduced hypothalamic PGEâ‚‚ biosynthesis in febrile COX-1 wild-type mice, but not in febrile COX-1 knockout mice. In conclusion, we provide further evidence which suggests that the hypothermic and now anti-pyretic actions of paracetamol are mediated through inhibition of a COX-1 variant enzyme
On the utility of alternative amino acid scripts
In this work we propose the hypothesis that replacing the current system of representing the chemical entities known as amino acids using Latin letters with one of several possible alternative symbolic representations will bring significant benefits to the human construction, modification, and analysis of multiple protein sequence alignments. We propose ways in which this might be done without prescribing the choice of actual scripts used. Specifically we propose and explore three ways to encode amino acid texts using novel symbolic alphabets free from precedents. Primary orthographic encoding is the direct substitution of a new alphabet for the standard, Latin-based amino acid code. Secondary encoding imposes static residue groupings onto the orthography of the alphabet by manipulating the shape and/or orientation of amino acid symbols. Tertiary encoding renders each residue as a composite symbol; each such symbol thus representing several alternative amino acid groupings simultaneously. We also propose that the use of a new group-focussed alphabet will free the colouring of amino acid residues often used as a tool to facilitate the representation or construction of multiple alignments for other purposes, possibly to indicate dynamic properties of an alignment such as position-wise residue conservation
Collisions of electrons with interstellar grains
We have computed cross-sections for elastic and inelastic scattering of electrons on small grains at low-collision energies. Significant differences are again found between the results obtained in the presence and the absence of a ‘permanent’ grain dipole moment. In addition to spherical grains, scattering on ellipsoidal grains is investigated. We conclude that the rate of electron attachment to interstellar grains may be substantially lower in regions of molecular clouds from which the radiation field is excluded, and where the grains are less likely to possess a dipole moment
The immune system as drug target
The immune system is perhaps the largest yet most diffuse and distributed somatic system in vertebrates. It plays vital roles in fighting infection and in the homeostatic control of chronic disease. As such, the immune system in both pathological and healthy states is a prime target for therapeutic interventions by drugs-both small-molecule and biologic. Comprising both the innate and adaptive immune systems, human immunity is awash with potential unexploited molecular targets. Key examples include the pattern recognition receptors of the innate immune system and the major histocompatibility complex of the adaptive immune system. Moreover, the immune system is also the source of many current and, hopefully, future drugs, of which the prime example is the monoclonal antibody, the most exciting and profitable type of present-day drug moiety. This brief review explores the identity and synergies of the hierarchy of drug targets represented by the human immune system, with particular emphasis on the emerging paradigm of systems pharmacology
Kinetics of the reaction of nitric oxide with hydrogen
Mixtures of NO and H2 diluted in argon or krypton were heated by incident shock waves, and the infrared emission from the fundamental vibration-rotation band of NO at 5.3 microns was used to monitor the time-varying NO concentration. The reaction kinetics were studied in the temperature range 2400-4500 K using a shock-tube technique. The decomposition of nitric oxide behind the shock was found to be modeled well by a fifteen-reaction system. A principle result of the study was the determination of the rate constant for the reaction H + NO yields N + OH, which may be the rate-limiting step for NO removal in some combustion systems. Experimental values of k sub 1 were obtained for each test through comparisons of measured and numerically predicted NO profiles
Shocks in dense clouds. IV. Effects of grain-grain processing on molecular line emission
Grain-grain processing has been shown to be an indispensable ingredient of
shock modelling in high density environments. For densities higher than
\sim10^5 cm-3, shattering becomes a self-enhanced process that imposes severe
chemical and dynamical consequences on the shock characteristics. Shattering is
accompanied by the vaporization of grains, which can directly release SiO to
the gas phase. Given that SiO rotational line radiation is used as a major
tracer of shocks in dense clouds, it is crucial to understand the influence of
vaporization on SiO line emission. We have developed a recipe for implementing
the effects of shattering and vaporization into a 2-fluid shock model,
resulting in a reduction of computation time by a factor \sim100 compared to a
multi-fluid modelling approach. This implementation was combined with an
LVG-based modelling of molecular line radiation transport. Using this model we
calculated grids of shock models to explore the consequences of different
dust-processing scenarios. Grain-grain processing is shown to have a strong
influence on C-type shocks for a broad range of magnetic fields: they become
hotter and thinner. The reduction in column density of shocked gas lowers the
intensity of molecular lines, at the same time as higher peak temperatures
increase the intensity of highly excited transitions compared to shocks without
grain-grain processing. For OH the net effect is an increase in line
intensities, while for CO and H2O it is the contrary. The intensity of H2
emission is decreased in low transitions and increased for highly excited
lines. For all molecules, the highly excited lines become sensitive to the
value of the magnetic field. Although vaporization increases the intensity of
SiO rotational lines, this effect is weakened by the reduced shock width. The
release of SiO early in the hot shock changes the excitation characteristics of
SiO radiation.Comment: Published in Astronomy and Astrophysics (2013). 26 pages, 16 figures,
14 table
SiO line emission from C-type shock waves : interstellar jets and outflows
We study the production of SiO in the gas phase of molecular outflows,
through the sputtering of Si--bearing material in refractory grain cores, which
are taken to be olivine; we calculate also the rotational line spectrum of the
SiO. The sputtering is driven by neutral particle impact on charged grains, in
steady--state C-type shock waves, at the speed of ambipolar diffusion. The
emission of the SiO molecule is calculated by means of an LVG code. A grid of
models has been generated. We compare our results with those of an earlier
study (Schilke et al. 1997). Improvements in the treatment of the coupling
between the charged grains and the neutral fluid lead to narrower shock waves
and lower fractions of Si being released into the gas phase. More realistic
assumptions concerning the initial fractional abundance of O2 lead to SiO
formation being delayed, so that it occurs in the cool, dense postshock flow.
Good agreement is obtained with recent observations of SiO line intensities in
the L1157 and L1448 molecular outflows. The inferred temperature, opacity, and
SiO column density in the emission region differ significantly from those
estimated by means of LVG `slab' models. The fractional abundance of SiO is
deduced. Observed line profiles are wider than predicted and imply multiple,
unresolved shock regions within the beam.Comment: 1 tex doc, 19 figure
Discriminating antigen and non-antigen using proteome dissimilarity: bacterial antigens
It has been postulated that immunogenicity results from the overall dissimilarity of pathogenic proteins versus the host proteome. We have
sought to use this concept to discriminate between antigens and non-antigens of bacterial origin. Sets of 100 known antigenic and nonantigenic
peptide sequences from bacteria were compared to human and mouse proteomes. Both antigenic and non-antigenic sequences
lacked human or mouse homologues. Observed distributions were compared using the non-parametric Mann-Whitney test. The statistical
null hypothesis was accepted, indicating that antigen and non-antigens did not differ significantly. Likewise, we were unable to determine a
threshold able to separate meaningfully antigen from non-antigen. Thus, antigens cannot be predicted from pathogen genomes based solely
on their dissimilarity to the human genome
- …