A comparison of lead-based and lead-free bullets for shooting sambar deer (Cervus unicolor) in Australia

Context. In response to the toxic health threats posed by lead (Pb), there is currently a focus on transitioning to lead-free bullets for shooting wild animals. Aim. We aimed to quantify the killing efficiency and animal welfare outcomes of lead-based and lead-free (copper-based) bullets for ground-based shooting of sambar deer (Cervus unicolor) in Victoria, south-eastern Australia. Methods. We used shooter-collected data from recreational diurnal hunting and professional nocturnal culling during 2020–2021. Shooters recorded rifle calibre, cartridge type, bullet mass, bullet type, shooting outcomes (miss, wound or kill), shooting distance, flight distance (the distance between where the animal was shot and where it died) as an assumed positive correlate of time to incapacitation, anatomical zones struck by bullets, and frequency of bullet exit wounds. We used flight distance as our response variable, assuming that it is positively correlated with time to incapacitation. To examine the role of several predictor variables (including bullet type) potentially influencing flight distance, the dataset was reduced to those deer killed with a single thoracic shot. Key results. Our data captured shooting events involving 276 deer, with 124 deer shot at with lead-based bullets and 152 with copper-based bullets. Most (87%) of the deer were killed with a single shot. The frequency of non-fatal wounding was <4% for both bullet types and there was no distinct difference in the probability of a single shot kill for deer shot with either bullet type. For those deer killed with a single thoracic shot (n = 198), there was no evidence that bullet energy or shooting distance influenced flight distance. After accounting for differences in terminal kinetic energy, the mean flight distance of deer shot with lead-free bullets (35 m) was 56% greater than that of deer shot with lead-based bullets (22 m). Conclusions. Lead-based and lead-free bullets produced similar animal welfare outcomes for shooting sambar deer. Implications. A transition to lead-free ammunition for shooting sambar deer would have minimal impact on efficiency or animal welfare outcomes. animal welfare, culling, human dimensions, invasive species, population control, recreational hunting, toxicology, wildlife management.publishedVersio

A comparison of lead-based and lead-free bullets for shooting sambar deer (Cervus unicolor) in Australia

Context: In response to the toxic health threats posed by lead (Pb), there is currently a focus on transitioning to lead-free bullets for shooting wild animals. Aim: We aimed to quantify the killing efficiency and animal welfare outcomes of lead-based and lead-free (copper-based) bullets for ground-based shooting of sambar deer (Cervus unicolor) in Victoria, south-eastern Australia. Methods: We used shooter-collected data from recreational diurnal hunting and professional nocturnal culling during 2020–2021. Shooters recorded rifle calibre, cartridge type, bullet mass, bullet type, shooting outcomes (miss, wound or kill), shooting distance, flight distance (the distance between where the animal was shot and where it died) as an assumed positive correlate of time to incapacitation, anatomical zones struck by bullets, and frequency of bullet exit wounds. We used flight distance as our response variable, assuming that it is positively correlated with time to incapacitation. To examine the role of several predictor variables (including bullet type) potentially influencing flight distance, the dataset was reduced to those deer killed with a single thoracic shot. Key results: Our data captured shooting events involving 276 deer, with 124 deer shot at with lead-based bullets and 152 with copper-based bullets. Most (87%) of the deer were killed with a single shot. The frequency of non-fatal wounding was <4% for both bullet types and there was no distinct difference in the probability of a single shot kill for deer shot with either bullet type. For those deer killed with a single thoracic shot (n = 198), there was no evidence that bullet energy or shooting distance influenced flight distance. After accounting for differences in terminal kinetic energy, the mean flight distance of deer shot with lead-free bullets (35 m) was 56% greater than that of deer shot with lead-based bullets (22 m). Conclusions: Lead-based and lead-free bullets produced similar animal welfare outcomes for shooting sambar deer. Implications: A transition to lead-free ammunition for shooting sambar deer would have minimal impact on efficiency or animal welfare outcomes

Evidence that breast cancer risk at the 2q35 locus is mediated through IGFBP5 regulation.

GWAS have identified a breast cancer susceptibility locus on 2q35. Here we report the fine mapping of this locus using data from 101,943 subjects from 50 case-control studies. We genotype 276 SNPs using the 'iCOGS' genotyping array and impute genotypes for a further 1,284 using 1000 Genomes Project data. All but two, strongly correlated SNPs (rs4442975 G/T and rs6721996 G/A) are excluded as candidate causal variants at odds against >100:1. The best functional candidate, rs4442975, is associated with oestrogen receptor positive (ER+) disease with an odds ratio (OR) in Europeans of 0.85 (95% confidence interval=0.84-0.87; P=1.7 × 10(-43)) per t-allele. This SNP flanks a transcriptional enhancer that physically interacts with the promoter of IGFBP5 (encoding insulin-like growth factor-binding protein 5) and displays allele-specific gene expression, FOXA1 binding and chromatin looping. Evidence suggests that the g-allele confers increased breast cancer susceptibility through relative downregulation of IGFBP5, a gene with known roles in breast cell biology

Publisher Correction: Evidence that breast cancer risk at the 2q35 locus is mediated through IGFBP5 regulation.

This corrects the article DOI: 10.1038/ncomms5999

Nutritional requirements of pregnant and lactating pregnant and lactating fallow deer (Dama dama)

This thesis describes a number of experiments undertaken to assess the nutritional requirements of pregnant and lactating fallow does with the aim of enhancing production and Quality Assurance in the Australian Deer Industry.Areas of study include determination of metabolisable energy intake of farmed fallow deer does of two genotypes throughout pregnancy and lactation, metabolisable energy intake of fallow deer fawns from 12 to 20 weeks of age and the effects of restricted maternal nutrition on foetal and placental development at different stages of gestation.In conjunction with nutritional adequacy, a body condition scoring system based on ante-mortem and post-mortem descriptors was developed for fallow deer. The results of this research make possible the precise strategic feeding of fallow deer breeding stock, which should lead to more consistent reproductive performance and higher quality slaughter animals. Furthermore, use of strategic feeding in conjunction with BCS systems will lead to better resource management and profitability, as farmers consistently produce animals to specificatio

The effect of pelvic suspension on the biochemical and sensory quality of venison from red deer (Cervus elaphus) and fallow deer (Dama dama)

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Rate of blood loss and timing of exsanguination on prevalence of ecchymosis in fallow deer (Dama dama)

Blood loss associated with four combinations of stunning and exsanguination methods was determined as part of studying prevalence of ecchymosis in slaughtered fallow deer (Dama dama). A fifth treatment simulated incomplete severance of the extended neck after captive bolt stunning. Predicted mean weights of blood collected from the five slaughter treatments, including electrical stunning followed by the thoracic stick method of exsanguination, captive bolt stunning followed by the thoracic stick, captive bolt stunning followed by the gash cut method of exsanguination, electrical stunning followed by the gash cut, and captive bolt stunning followed by incomplete severance of the extended neck, were 1458.7, 1072.7, 684.5, 463.7, and 228.5 g, respectively. The overall effect of exsanguination method was highly significant (p < 0.001) with the thoracic stick resulting in the greatest weight of blood collection in the 10 s period immediately after initiation. The overall effect of stunning method on blood loss was not significant, although there was a significant (p < 0.05) stunning method by exsanguination method interaction. Ecchymosis of varying severity occurred in some carcases from all treatment groups. Using the total loin and round ecchymosis scores, when little or no ecchymosis (grades 0 or 1) was compared with some ecchymosis (Pgrade 2) by logistic regression, castrated bucks were 9.8 times more likely (p = 0.002) and does 4.2 times more likely (p = 0.06) to have some ecchymosis than entire bucks. The results indicate that ecchymosis can be reduced in fallow deer carcases by thoracic stick exsanguination incorporated less than 10 s after stunning

Consumer evaluation of venison sensory quality : effects of sex, body condition score and carcase suspension method

Intact fallow bucks (n=20) and non-pregnant fallow does (n=24) (Dama dama), in the body condition score (BCS) of 2, 3 and 4 (lean, prime and fat) and rising 2 year old red deer stags (n=26) (Cervus elaphus) of the same BCS range (2-4) were used in this study to determine the impact of sex, BCS and method of carcase suspension on consumer perception of venison quality. Consumers were asked to evaluate cooked meat samples (M. gluteus medius) on an unstructured line scale for colour, flavour, tenderness, juiciness and overall liking. Meat from both fallow deer and red deer was preferred by consumers when carcases had hung by the pelvic suspension (PS) method compared with the Achilles tendon (AT) method of hanging (p<0.001). Consumers also noted a difference in colour between sexes in fallow deer venison, with venison from 36 month-old does being darker (p=0.015), and preferred venison from does over 18-24 month-old bucks. There was a significant difference in the consumer scores for tenderness in red deer stags of BCSs 2 and 4 (p=0.05) with panellists determining BCS 4 animals to be more tender; however no tenderness differences were observed for fallow deer does compared with bucks

A simple method of attaching GPS tracking devices to free-ranging Lace Monitors (Varanus varius)

Effective and reliable methods of attachment for very high frequency (VHF) transmitters to animals are essential for radio-telemetric studies. However, attachment difficulties escalate when longer term studies requiring larger global positioning system (GPS) loggers and additional battery life are required, resulting in the use of larger, more cumbersome units. Lace Monitors (Varanus various) of Australia inhabit rugged terrain in the wild, and accurate portrayal of home range and temporal patterns of movement requires a remote data collection process. We developed a robust system of attaching GPS loggers to Lace Monitors, while allowing them to attain a full range of movement, level of activity, and foraging methods. We designed a denim pouch to house a large GPS logger with radio-transmitter, and this unit was then glued to the back of adult Lace Monitors. Fifteen males fitted with denim pouches containing GPS loggers were successfully tracked for 6–65 weeks to study home range and habitat utilization. None of the denim pouches were prematurely dislodged from the animals during the study and pouch design did not appear to affect the natural behavior of the animals. This paper provides a detailed description of the pouch design and the efficacy of its attachment