A reporter for amyloid precursor protein γ-secretase activity in Drosophila

A key event in the pathogenesis of Alzheimer's disease (AD) is the deposition of senile plaques consisting largely of a peptide known as β-amyloid (Aβ) that is derived from the amyloid precursor protein (APP). A proteolytic activity called γ-secretase cleaves APP in the transmembrane domain and is required for Aβ generation. Aberrant γ-secretase cleavage of APP underlies the majority of early onset, familial AD. γ-Secretase resides in a large multi-protein complex, of which Presenilin, Nicastrin, APH-1 and PEN-2 are four essential components. Thus, identifying components and pathways by which the γ-secretase activity is regulated is crucial to understanding the mechanisms underlying AD pathogenesis, and may provide new diagnostic tools and therapeutic targets. Here we describe the generation of Drosophila that act as living reporters of γ-secretase activity in the fly eye. In these reporter flies the size of the eye correlates with the level of endogenous γ-secretase activity, and is very sensitive to the levels of three genes required for APP γ-secretase activity, presenilin, nicastrin and aph-1. Thus, these flies provide a sensitized system with which to identify other components of the γ-secretase complex and regulators of its activity. We have used these flies to carry out a screen for mutations that suppress γ-secretase activity and have identified a small chromosomal region that contains a gene or genes whose products may promote γ-secretase activity

DocGenealogy: uma árvore genealógica de doutorados

Poster mostrado no EPCG sobre a árvore genealógica existente entre orientados e orientadores, extraído à partir da co-relação das infoboxes da Wikipedia.N/

Visualização de informação

O relatório está dividido em duas partes. Na primeira parte, é abordado o problema da visualização exactamente no que diz respeito à subtil correlação existente entre as técnicas (e respectivas metáforas), o utilizador e os dados. Na segunda parte, são analisadas algumas aplicações, projectos, ferramentas e sistemas de Visualização de Informação. Para categorizalos, serão considerados sete tipos de dados básicos subjacentes a eles: unidimensional, bidimensional, tridimensional, multi-dimensional, temporal, hierárquico, rede e workspace.O tema deste relatório é a visualização da informação. Esta é uma área actualmente muito activa e vital no ensino, na pesquisa e no desenvolvimento tecnológico. A ideia básica é utilizar imagens geradas pelo computador como meio para se obter uma maior compreensão e apreensão da informação que está presente nos dados (geometria) e suas relações (topologia). É um conceito simples, porém poderoso que tem criado imenso impacto em diversas áreas da engenharia e ciência.The theme of this report is information visualization. Nowadays, this is a very active and vital area of research, teaching and development. The basic idea of using computer generated pictures to gain information and understanding from data and relationships is the key concept behind it. This is an extremely simple, but very important concept which is having a powerful impact on methodology of engineering and science. This report is consisted of two parts. The first one, is an overview of the subtle correlation between the visual techniques, the user perception and the data. In the second part, several computer applications, tools, projects and information visualization systems are analyzed. In order to categorize them, seven basic types of data are considered: onedimensional, two- dimensional, three-dimensional, multidimensional, temporal, hierarchic, network and workspace

As instituições e as leis para a infância no Brasil Império: circulação de ideias sobre o menorismo

O artigo realiza uma pesquisa documental em leis, normas, decretos e regulamentos que constituem fontes primárias, datadas cronologicamente no século XIX, com vistas a responder à seguinte problematização: em que momento o termo menor passou a caracterizar um etiquetamento para meninos e meninas que viviam no Brasil império e a constituir uma base para as práticas tutelares que ganharam ênfase no século XX? O estudo objetiva demonstrar que o Código de Menores de 1927 foi promulgado como resultado do menorismo e parte de um movimento transnacional que nasceu nos Estados Unidos da América e circulou na Europa e na América Latina. Diferentemente do que se pressupunha, a investigação para a elaboração do texto concluiu que o termo menor – no sentido menorista – já era utilizado, no Brasil, escassamente em normativas a partir de 1850, ou seja, quase 80 anos antes do que as fontes e as pesquisas realizadas apontavam, sendo o Código de Menores o efeito do menorismo e não o seu criador. Palavras-chave: Infância. Menorismo. Código de Menores. Práticas tutelares

Data Science, Machine learning and big data in Digital Journalism: A survey of state-of-the-art, challenges and opportunities

Digital journalism has faced a dramatic change and media companies are challenged to use data science algo-rithms to be more competitive in a Big Data era. While this is a relatively new area of study in the media landscape, the use of machine learning and artificial intelligence has increased substantially over the last few years. In particular, the adoption of data science models for personalization and recommendation has attracted the attention of several media publishers. Following this trend, this paper presents a research literature analysis on the role of Data Science (DS) in Digital Journalism (DJ). Specifically, the aim is to present a critical literature review, synthetizing the main application areas of DS in DJ, highlighting research gaps, challenges, and op-portunities for future studies. Through a systematic literature review integrating bibliometric search, text min-ing, and qualitative discussion, the relevant literature was identified and extensively analyzed. The review reveals an increasing use of DS methods in DJ, with almost 47% of the research being published in the last three years. An hierarchical clustering highlighted six main research domains focused on text mining, event extraction, online comment analysis, recommendation systems, automated journalism, and exploratory data analysis along with some machine learning approaches. Future research directions comprise developing models to improve personalization and engagement features, exploring recommendation algorithms, testing new automated jour-nalism solutions, and improving paywall mechanisms.Acknowledgements This work was supported by the FCT-Funda?a ? o para a Ciência e Tecnologia, under the Projects: UIDB/04466/2020, UIDP/04466/2020, and UIDB/00319/2020

Parents' experience of children with acquired brain injury undergoing neurorehabilitation:a systematic review

BACKGROUND: This systematic review aims to synthesise the qualitative evidence exploring parents' experiences of children with acquired brain injury (ABI) undergoing neurorehabilitation during the first year post-injury.METHODS: A systematic review of qualitative research was conducted using thematic synthesis with Thomas and Harden's approach. The population, exposure and outcome model was used for the search strategy. The electronic databases Ovid Embase, Ovid MEDLINE, CINAHL, Scopus and PsycINFO were searched from 2009 to 2023. The review included qualitative and mixed-method studies published in English only. Grey literature was excluded. There were no geographical restrictions. Reporting within the review followed the Enhancing Transparency in Reporting the Synthesis of Qualitative Research guideline. The studies' quality was appraised using the Critical Appraisal Skills Programme tool.RESULTS: Three studies met the inclusion criteria and were included in the synthesis, representing the experiences of 30 parents. The quality assessment showed that the three included studies met most quality indicators. Following thematic synthesis, four analytical themes were identified: school unpreparedness, parents as advocates and navigators, parents as monitors, and parents recognising the impact of ABI on their child. The reviewers proposed a group of recommendations for services reviewing their parental support.CONCLUSION: This review highlights some challenges parents of children diagnosed with ABI experience during their child's neurorehabilitation journey. This review has suggested potential improvements that could be made in paediatric neurorehabilitation services when reviewing their parental support and care pathways. These will ultimately influence parents' and children's experience of paediatric neurorehabilitation services.</p

Negotiating the Sacred II

Blasphemy and other forms of blatant disrespect to religious beliefs have the capacity to create significant civil and even international unrest. Consequently, the sacrosanctity of religious dogmas and beliefs, stringent laws of repression and codes of moral and ethical propriety have compelled artists to live and create with occupational hazards like uncertain audience response, self-censorship and accusations of deliberate misinterpretation of cultural production looming over their heads. Yet, in recent years, issues surrounding the rights of minority cultures to recognition and respect have raised new questions about the contemporariness of the construct of blasphemy and sacrilege. Controversies over the aesthetic representation of the sacred, the exhibition of the sacred as art, and the public display of sacrilegious or blasphemous works have given rise to heated debates and have invited us to reflect on binaries like artistic and religious sensibilities, tolerance and philistinism, the sacred and the profane, deification and vilification. Endeavouring to move beyond ‘simplistic’ points about the rights to freedom of expression and sacrosanctity, this collection explores how differences between conceptions of the sacred can be negotiated. It recognises that blasphemy may be justified as a form of political criticism, as well as a sincere expression of spirituality. But it also recognises that within a pluralistic society, blasphemy in the arts can do an enormous amount of harm, as it may also impair relations within and between societies. This collection evolved out a two-day conference called ‘Negotiating the Sacred: Blasphemy and Sacrilege in the Arts’ held at the Centre for Cross Cultural Research at The Australian National University in November 2005. This is the second volume in a series of five conferences and edited collections on the theme ‘Negotiating the Sacred’. The first conference, ‘Negotiating the Sacred: Blasphemy and Sacrilege in a Multicultural Society’ was held at The Australian National University’s Centre for Cross-Cultural Research in 2004, and published as an edited collection by ANU E Press in 2006. Other conferences in the series have included Religion, Medicine and the Body (ANU, 2006), Tolerance, Education and the Curriculum (ANU, 2007), and Governing the Family (Monash University, 2008). Together, the series represents a major contribution to ongoing debates on the political demands arising from religious pluralism in multicultural societies

Dual-pool, three-phase kinetic model of anaerobic digestion in batch mode

Research ArticleThe ability of anaerobic digestion to create value from waste gives it an important role in reducing greenhouse gas emissions and in the transition to a circular economy. For a better understanding of the digestion process and in order to reduce the number of time-consuming batch tests, an analytical model was developed to describe the kinetics of biogas production. Assuming that the organic fraction of the substrate has different degradation rates, the whole process was modelled as two groups of 1storder reactions. The model was tested with published data and showed an excellent performance in reproducing the experimental information. Moreover, its kinetic constants provided a useful insight into the internal processes of anaerobic digestion and the substrate characteristics. Given its accuracy in fitting the data, the model can be used as an auxiliary tool to determine the biogas potential, presenting itself as the most complete empirical model currently availableinfo:eu-repo/semantics/publishedVersio

Avoiding disclosure of individually identifiable health information: a literature review

Achieving data and information dissemination without arming anyone is a central task of any entity in charge of collecting data. In this article, the authors examine the literature on data and statistical confidentiality. Rather than comparing the theoretical properties of specific methods, they emphasize the main themes that emerge from the ongoing discussion among scientists regarding how best to achieve the appropriate balance between data protection, data utility, and data dissemination. They cover the literature on de-identification and reidentification methods with emphasis on health care data. The authors also discuss the benefits and limitations for the most common access methods. Although there is abundant theoretical and empirical research, their review reveals lack of consensus on fundamental questions for empirical practice: How to assess disclosure risk, how to choose among disclosure methods, how to assess reidentification risk, and how to measure utility loss.public use files, disclosure avoidance, reidentification, de-identification, data utility