Design and Fabrication of Customized Tracheal Stents by Additive Manufacturing

Abstract Additive Manufacturing (AM) is already becoming part of our life from a technological, economic and social point of view. Nowadays, it is applied in several manufacturing sectors. In particular, AM shows huge opportunities in the medical field and for healthcare applications. Due to its capability to produce complex geometries directly working on medical 3D images and thanks to the possibility to 3D-print biocompatible materials, AM is a key technology for the fabrication both of external and internal medical devices. In particular, the use of AM for medical applications is typically articulated in three steps: 3D-scanning of the patient anatomy, segmentation the medical scan and elaboration through CAD software for the preparation of a STL file suitable for the AM process. One of the main research topic in this field is the definition and optimization of procedures that, taking precise data from an individual patient, could be applied to the design and fabrication of customized components for medical applications. Therefore, this paper presents a project aimed at the fabrication of customized tracheal stents starting from the actual patient anatomy. In particular, it follows an approach based on molds FDM fabrication followed by biocompatible silicone casting. Molds were designed to obtain a tracheal stent based the patient anatomical tracheal lumen and were fabricated using FDM technology. Moreover, since the surface roughness is one of the most critical aspects related to the FDM, the produced molds were finished with a chemical surface post-treatment based on the use of acetone vapours. Overall, the whole developed procedure results in an effective custom-made medical devices realization

TOXICITY OF PREDNISOLONE, DEXAMETHASONE AND THEIR PHOTOCHEMICAL DERIVATIVES ON AQUATIC ORGANISMS

Light exposure of aqueous suspensions of prednisolone and dexamethasone causes their partial phototransformation. The photoproducts, isolated by chromatographic techniques, have been identified by spectroscopic means. Prednisolone, dexamethasone and their photoproducts have been tested to evaluate their acute and chronic toxic effects on some freshwater chain organisms. The rotifer Brachionus calyciflorus and the crustaceans Thamnocephalus platyurus and Daphnia magna were chosen to perform acute toxicity tests, while the alga Pseudokircheneriella subcapitata (formerly known as Selenastrum capricornutum) and the crustacean Ceriodaphnia dubia to perform chronic tests. The photochemical derivatives are more toxic than the parent compounds. Generally low acute toxicity was found. Chronic exposure to this class of pharmaceuticals caused inhibition of growth population on the freshwater crustacean C. dubia while the alga P. subcapitata seems to be less affected by the presence of these drugs

Antibiotic resistance in stream: monitoring, modeling and effluent control by photocatalytic disinfection

2013-2014Since the 1940s, the ever-increasing use of antibiotics for human, veterinary and agricultural purposes, contributes to their continuous release into the environment due to incomplete metabolism or due to disposal of unused antibiotics. The concern for the release of antibiotics into the environment isrelated to the development of antibiotic resistance genes (ARGs) and bacteria (ARB), which reduce the therapeutic potential against human and animal pathogens. Urban wastewater treatment plant (UWWTP) effluents, hospital discharges, livestock farms represent today the major contamination sources of surface water from antibiotics and ARB. The consequence is that antibiotics, exerting selective pressure, may facilitate the selection of ARB or the acquisition of resistance genes by horizontal transfer. The aim of this work was to investigate the spread of ARB in the environment, particularly in water system, as well as to minimize the related risk through the investigation of effective wastewater disinfection methods. Accordingly, experimental activity was addressed to (i) the monitoring of ARB in river, (ii) modelling ARB fate in river and (iii) minimize ARB release in river through effective wastewater disinfection. [edited by author]XIII n.s

Adult-type rhabdomyoma of the larynx: clinicopathologic study of an uncommon tumor in a rare location

Rhabdomyoma is an uncommon benign mesenchymal tumor with skeletal muscle differentiation that may occur either in the heart or in extra-cardiac sites. Even though the head and neck region is the most common area of extra-cardiac rhabdomyoma, the larynx is rarely involved. We present the case of an 85-year-old woman who reported a 10-day history of breathing difficulties, dysphagia and dysphonia. A computed tomography scan of the head and neck showed a contrast-enhanced, solid hypopharyngeal-laryngeal neoplasm with well-defined margins causing subtotal obliteration of the right pyriform sinus and a reduction in air lumen of the laryngeal vestibule. The patient underwent complete endoscopic removal of the lesion; histologic examination revealed an adult- type rhabdomyoma based on the histologic features and the immunoreactivity of the neoplastic cells for desmin, myoglobin and muscle-specific actin but not for cytokeratin, S-100, CD68R, chromogranin-A and synaptophysin. Since clinical and imaging features are not specific for rhabdomyoma, histologic examination and immunohistochemical analyses play a central role in the differential diagnosis of the adult-type rhabdomyoma from other laryngeal neoplasms. A correct diagnosis is mandatory to avoid inappropriate treatment

Chimneys in wooden roofs: a 3D steady numerical model for the prediction of the temperatures

Chimneys convey exhaust gas produced in domestic heat appliances to the external environment and to do this they have to pass through floors and roofs: if these elements are made up of flammable materials, the fire hazard may occur. This article presents the verification of a 3D numerical model set for the determination of the stationary temperatures reached on the flammable materials in the vicinity of chimneys. The verification has been done comparing the temperatures measured in experimental tests with those estimated by means of the numerical model. Results show that the 3D numerical model is a valid tool for the analysis of heat transfer between chimney and roof. Since in the experimental tests it is not always possible to reach stationary temperatures, a lumped element model to estimate them is also proposed and it could support the chimney certification

Development and validation of a microbiological assay for determination of chlorhexidine digluconate in aqueous solution

Chlorhexidine (CHX) is a broad-spectrum antiseptic that is used in many topical pharmaceutical formulations. Because there is no official microbiological assay reported in the literature that is used to quantify CHX, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method for the dosage of chlorhexidine digluconate (CHX-D) in an aqueous solution. The assay is based on the inhibitory effect of CHX-D upon the strain of Staphylococcus aureus ATCC 25923, which is used as the test microorganism. The design 3x3 parallel-line model was used. The results were treated statistically by analysis of variance (ANOVA), and they were excellent in terms of linearity (r = 0.9999), presenting a significant regression between the zone diameter of growth inhibition and the logarithm of the concentration within the range of 0.5 to 4.5%. The results obtained were precise, having relative standard deviations (RSD) for intra-day and inter-day precision of 2.03% and 2.94%, respectively. The accuracy was 99.03%. The method proved to be very useful and appropriate for the microbiological dosage of CHX-D in pharmaceutical formulations; it might also be used for routine drug analysis during quality control in pharmaceutical industries.Clorexidina (CHX) é um antisséptico com amplo espectro de ação utilizada em muitos tipos de preparações farmacêuticas para uso tópico. Uma vez que não há na literatura ensaio microbiológico oficial para quantificar a clorexidina, este trabalho objetivou o desenvolvimento e validação de um ensaio microbiológico simples, sensível, exato e reprodutível, por difusão em ágar, para doseamento de digliconato de clorexidina (CHX-D) em solução aquosa. O ensaio é baseado no efeito da inibição de Staphylococcus aureus ATCC 25923, utilizado como microorganismo teste, pela CHX-D. Utilizou-se o delineamento 3x3. Os resultados foram verificados estatisticamente pela análise de variância (ANOVA) e apresentaram excelente linearidade (r = 0,9999), demonstrando que o método segue o modelo linear com regressão significativa entre o diâmetro da zona de inibição e o lagaritmo da concentração no intervalo de 0,5 a 4,5%. Os resultados obtidos foram precisos apresentando desvio padrão relativo (DPR) para precisão intra-dia de 2,03% e DPR para precisão inter-dias de 2,94%. A exatidão foi 99,03%. O método provou ser muito útil e apropriado para doseamento microbiológico da CHX-D em formas farmacêuticas e pode ser empregado para análise desta substância no controle de qualidade em indústrias farmacêuticas

Polyetherimide(PEI)/SiO2 organic/inorganic composite: sol-gel synthesis, structural characterization, surface interactions

Polyetherimide (PEI), an amorphous thermoplastic material is a promising candidate for wide applications due to its high heat stability and its biocompatibility in human tissue. In the present paper, PEI (4 wt%) was added to SiO2 inorganic matrix in order to obtain a novel composite biomaterial through sol-gel route. Structural characterization of the biomaterial was provided by Fourier transform infrared spectroscopy (FTIR) that confirmed the presence of both organic and inorganic components in the structure. A theoretical study based on Molecular Mechanics and Molecular Dynamics methods will be useful in order to better understand the intermolecular interaction at the organic/inorganic interface compared with the discussed structural characterization. Concerning the compatibility in the biological system, a study of antibacterial properties was carried out. The effect of PEI/SiO2 composite on gram-negative bacterium Escherichia coli, was analyzed with a marked antimicrobial activity